Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins
- Citation:
- Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins, Rial-Otero, R., Carreira R. J., Cordeiro F. M., Moro A. J., Fernandes L., Moura I., and Capelo J. L. , Journal of Proteome Research, Feb, Volume 6, Number 2, p.909-912, (2007)
Abstract:
Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylylsulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the methodology. The new sample treatment is of easy implementation, saves time and money, and can be adapted to online procedures and robotic platforms.
Notes:
Times Cited: 20
