Simulation of the electrochemical behavior of multi-redox systems. Current potential studies on multiheme cytochromes
- Citation:
- Simulation of the electrochemical behavior of multi-redox systems. Current potential studies on multiheme cytochromes, Moreno, C., Campos A., Teixeira M., Legall J., Montenegro M. I., Moura I., Van Dijk C., and Moura J. G. , Eur J Biochem, Dec 5, Volume 202, Number 2, p.385-93, (1991)
Abstract:
The direct unmediated electrochemical response of the tetrahemic cytochrome c3 isolated from sulfate reducers Desulfovibrio baculatus (DSM 1743) and D. vulgaris (strain Hildenborough), was evaluated using different electrode systems [graphite (edge cut), gold, semiconductor (InO2) and mercury)] and different electrochemical methods (cyclic voltammetry and differential pulse voltammetry). A computer program was developed for the theoretical simulation of a complete cyclic voltammetry curve, based on the method proposed by Nicholson and Shain [Nicholson, R.S. & Shain, I. (1964) Anal. Chem. 36, 706-723], using the Gauss-Legendre method for calculation of the integral equations. The experimental data obtained for this multi-redox center protein was deconvoluted in to the four redox components using theoretically generated cyclic voltammetry curves and the four mid-point reduction potentials determined. The pH dependence of the four reduction potentials was evaluated using the deconvolution method described.
Notes:
0014-2956 (Print)0014-2956 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
