Resonance Raman spectra of three-iron centers in ferredoxins from Desulfovibrio gigas
- Citation:
- Resonance Raman spectra of three-iron centers in ferredoxins from Desulfovibrio gigas, Johnson, M. K., Hare J. W., Spiro T. G., Moura J. J., Xavier A. V., and Legall J. , J Biol Chem, Oct 10, Volume 256, Number 19, p.9806-8, (1981)
Abstract:
The resonance Raman spectra of ferredoxins (Fd) I and II from Desulfovibrio gigas are reported using 4579 A Ar+ laser excitation. The (3Fe-3S) center in Fd II has a characteristic resonance Raman spectrum, readily distinguishable from those of (2Fe-2S) or (4Fe-4S) clusters. Reduction of Fd II produces a marked alteration in the resonance Raman spectrum. Fd I is shown to contain both (3Fe-3S) and (4Fe-4S) Fd-type clusters. The results illustrate the potential of resonance Raman spectroscopy in Fe-S cluster identification, even in cases where more than one cluster type is present.
Notes:
0021-9258 (Print)0021-9258 (Linking)Journal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.
