Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass Spectrometry
- Citation:
- Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass Spectrometry, Santos, H. M., Rial-Otero R., Fernandes L., Vale G., Rivas M. G., Moura I., and Capelo J. L. , Journal of Proteome Research, Sep, Volume 6, Number 9, p.3393-3399, (2007)
Abstract:
Three ultrasonic energy sources were studied to speed up the sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein reduction, alkylation, and enzymatic digestion steps were done in 15 min. Nine proteins, including zinc resistance-associated protein precursor from Desulfovibrio desulfuricans strain G20 and split-soret cytochrome c from D. desulfuricans ATCC27774 were successfully identified with the new protocol.
Notes:
Times Cited: 10
