Hydrogen evolution and consumption in AOT-isooctane reverse micelles by Desulfovibrio gigas hydrogenase
- Citation:
- Hydrogen evolution and consumption in AOT-isooctane reverse micelles by Desulfovibrio gigas hydrogenase, Andrade, S. L. A., and Moura J. J. G. , Enzyme and Microbial Technology, Sep 2, Volume 31, Number 4, p.398-402, (2002)
Abstract:
The enzyme hydrogenase isolated from the sulphate reducing anaerobic bacterium Desulfovibrio gigas was encapsulated in reverse micelles of AOT-water-isooctane. The enzyme ability to consume molecular hydrogen was studied as a function of the micelle size (given by W-o = [H2O]/[organic solvent]). A peak of catalytic activity was obtained for W-o = 18, a micelle size theoretically fitting the heterodimeric hydrogenase molecule. At this W-o value, the recorded catalytic activity was slightly higher than in a buffer system (K-cat = 169.43 s(-1) against the buffer value of 151 s(-1)). The optimal buffer used to encapsulate the enzyme was found to be imidazole 50 mM, pH 9.0, The molecular hydrogen production activity was also tested in this reverse micelle medium. (C) 2002 Elsevier Science Inc. All lights reserved.
Notes:
Times Cited: 3
