Encapsulation of flavodoxin in reverse micelles
- Citation:
- Encapsulation of flavodoxin in reverse micelles, Andrade, S., Kamenskaya E. O., Levashov A. V., and Moura J. J. , Biochem Biophys Res Commun, May 29, Volume 234, Number 3, p.651-4, (1997)
Abstract:
The regulation of the properties of Desulfovibrio gigas flavodoxin in AOT/water/iso-octane micellar system was studied. UV-visible spectroscopic studies have shown that photoreduction of flavodoxin in the presence of EDTA leads to hydroquinone formation through the intermediate semiquinone. The [free FMN] - [bound to flavodoxin FMN] equilibrium (and hence, the amount of apoprotein) depends on redox state of FMN and on hydration degree which controls the micellar size. Thus, a new method of reversible cofactor removing under mild conditions (at low hydration degree of micelles) is suggested, accompained by isolation of apo-form of the protein.
Notes:
0006-291X (Print)0006-291X (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
