Alejandro Samhan-Arias

Post-Doctoral Research Fellow, FCT-UNL
Email: alejandro.samhan(at)
Tel: + 351 21 294 83 00, ext. 10927
Webpage: webpage

Research Activities - Keywords
• Metalloenzymes, flavoproteins, structure-activity relationships
• Reactive oxygen and nitrogen species (ROS/RNS) biochemistry
    - formation/elimination and enzymes structure-activity relationships
• Mechanisms of ROS/RNS-mediated diseases
• Cytochrome b5 reductase (rat, pig and human), cytochrome b5, cytochrome c
• Plasma membrane NAD(P)H oxidases
• Reconstitution of electron transfer chains
• Molecular modeling (protein-protein interactions, protein-ligand interactions)
• Oxidative lipidomics

Teaching Interests
• Biochemistry and Molecular Biology
• Science divulgation

Recent Publications can be found at or here

Selected References
Correlation between the potency of flavonoids for cytochrome c reduction and inhibition of cardiolipin-induced peroxidase activity
Lagoa R, Samhan-Arias AK, Gutierrez-Merino C
Free Rad Biol Med (2017) (DOI: 10.1002/biof.1357)

Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite
Samhan-Arias AK, Gutierrez-Merino C
Free Rad Biol Med (2014) 73:174-189 (DOI: 10.1016/j.freeradbiomed.2014.04.033)

Lipidomics identifies cardiolipin oxidation as a mitochondrial target for redox therapy of brain injury
Ji J, Kline AE, Amoscato A, Samhan-Arias AK, Sparvero LJ, Tyurin VA, Tyurina YY, Fink B, Manole MD, Puccio AM, Okonkwo DO, Cheng JP, Alexander H, Clark RS, Kochanek PM, Wipf P, Kagan VE, Bayır H
Nature Neuroscience (2012) 15:1407-1413 (DOI: 10.1038/nn.3195)

Oxidized phospholipids as biomarkers of tissue and cell damage with a focus on cardiolipin
Samhan-Arias AK, Ji J, Demidova OM, Sparvero LJ, Feng W, Tyurin V, Tyurina YY, Epperly MW, Shvedova AA, Greenberger JS, Bayır H, Kagan VE, Amoscato AA
Biochim Biophys Acta (2012) 1818:2413-2423 (DOI: 10.1016/j.bbamem.2012.03.014)

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons
Samhan-Arias AK, Marques-da-Silva D, Yanamala N, Gutierrez-Merino C
J Proteomics (2012) 75:2934-2949 (DOI: 10.1016/j.jprot.2011.12.007)

A mitochondria-targeted inhibitor of cytochrome c peroxidase mitigates radiation-induced death
Atkinson J, Kapralov AA, Yanamala N, Tyurina YY, Amoscato AA, Pearce L, Peterson J, Huang Z, Jiang J, Samhan-Arias AK, Maeda A, Feng W, Wasserloos K, Belikova NA, Tyurin VA, Wang H, Fletcher J, Wang Y, Vlasova II, Klein-Seetharaman J, Stoyanovsky DA, Bayîr H, Pitt BR, Epperly MW, Greenberger JS, Kagan VE
Nat Commun (2011) 2:497 (DOI: 10.1038/ncomms1499)

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