@article {Gomes2010, title = {Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδT cells}, journal = {Haematologica}, volume = {95}, number = {8}, year = {2010}, pages = {1397{\textendash}1404}, abstract = {

BACKGROUND:
Vgamma9Vdelta2 T lymphocytes are regarded as promising mediators of cancer immunotherapy due to their capacity to eliminate multiple experimental tumors, particularly within those of hematopoietic origin. However, Vgamma9Vdelta2 T-cell based lymphoma clinical trials have suffered from the lack of biomarkers that can be used as prognostic of therapeutic success.
DESIGN AND METHODS:
We have conducted a comprehensive study of gene expression in acute lymphoblastic leukemias and non-Hodgkin{\textquoteright}s lymphomas, aimed at identifying markers of susceptibility versus resistance to Vgamma9Vdelta2 T cell-mediated cytotoxicity. We employed cDNA microarrays and quantitative real-time PCR to screen 20 leukemia and lymphoma cell lines, and 23 primary hematopoietic tumor samples. These data were analyzed using state-of-the-art bioinformatics, and gene expression patterns were correlated with susceptibility to Vgamma9Vdelta2 T cell mediated cytolysis in vitro.
RESULTS:
We identified a panel of 10 genes encoding cell surface proteins that were statistically differentially expressed between "gammadelta-susceptible" and "gammadelta-resistant" hematopoietic tumors. Within this panel, 3 genes (ULBP1, TFR2 and IFITM1) were associated with increased susceptibility to Vgamma9Vdelta2 T-cell cytotoxicity, whereas the other 7 (CLEC2D, NRP2, SELL, PKD2, KCNK12, ITGA6 and SLAMF1) were enriched in resistant tumors. Furthermore, some of these candidates displayed a striking variance of expression among primary follicular lymphomas and T-cell acute lymphoblastic leukemias.
CONCLUSIONS:
Our results suggest that hematopoietic tumors display a highly variable repertoire of surface proteins that can impact on Vgamma9Vdelta2 cell-mediated immunotargeting. The prognostic value of the proposed markers can now be evaluated in upcoming Vgamma9Vdelta2 T cell-based lymphoma/leukemia clinical trials.

}, keywords = {Biomarkers, Hematopoietic tumors, Lymphoma cell lines, V$\gamma$9v$δ$2 t-lymphocytes}, isbn = {1592-8721 (Electronic)$\backslash$r0390-6078 (Linking)}, issn = {03906078}, doi = {10.3324/haematol.2009.020602}, url = {https://doi.org/10.3324/haematol.2009.020602}, author = {Gomes, Anita Q and Correia, Daniel V. and Ana Rita Grosso and Lan{\c c}a, Telma and Ferreira, Cristina and Lacerda, Jo{\~a}o F. and Barata, Jo{\~a}o T. and da Silva, Maria Gomes and Silva-santos, Bruno} }