@article {Grosso2015,
	title = {Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma},
	journal = {eLife},
	volume = {4},
	year = {2015},
	pages = {e09214},
	abstract = {<p>Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Genome Atlas (TCGA) reveals that transcription read-through beyond the termination site is a source of transcriptome diversity in cancer cells. Amongst the genes most frequently mutated in ccRCC, we identified SETD2 inactivation as a potent enhancer of transcription read-through. We further show that invasion of neighbouring genes and generation of RNA chimeras are functional outcomes of transcription read-through. We identified the BCL2 oncogene as one of such invaded genes and detected a novel chimera, the CTSC-RAB38, in 20\% of ccRCC samples. Collectively, our data highlight a novel link between transcription read-through and aberrant expression of oncogenes and chimeric transcripts that is prevalent in cancer.</p>
},
	issn = {2050-084X},
	doi = {10.7554/eLife.09214},
	url = {https://doi.org/10.7554/eLife.09214},
	author = {Ana Rita Grosso and Leite, Ana Paula and Carvalho, S{\'\i}lvia and Matos, Mafalda Ramos and Martins, Filipa Batalha and V{\'\i}tor, Alexandra Coitos and Desterro, Joana Mp and Carmo-fonseca, Maria and de Almeida, S{\'e}rgio Fernandes}
}
