@article {13068,
	title = {Multifunctional microfluidic chip for optical nanoprobe based RNA detection {\textendash} application to Chronic Myeloid Leukemia},
	journal = {Scientific Reports},
	volume = {8},
	year = {2018},
	note = {<p>n/a</p>
},
	month = {2018},
	pages = {381},
	abstract = {<p>Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.</p>
},
	isbn = {2045-2322},
	url = {https://doi.org/10.1038/s41598-017-18725-9},
	author = {Pedro Urbano Alves and Raquel Vinhas and Alexandra R. Fernandes and Birol, Semra Zuhal and Trabzon, Levent and Iwona Bernacka-Wojcik and Rui Igreja and Paulo Lopes and Pedro Viana Baptista and Hugo {\'A}guas and Elvira Fortunato and Rodrigo Martins}
}
