<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Leal, Teresa Fontes</style></author><author><style face="normal" font="default" size="100%">Isabel de Sá-Nogueira</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">&lt;a href=&quot;http://onlinelibrary.wiley.com/doi/10.1016/j.femsle.2004.10.003/full&quot; target=&quot;_blank&quot;&gt;Purification, characterization and functional analysis of an endo-arabinanase (AbnA) from &lt;i&gt;Bacillus subtilis&lt;/i&gt;.&lt;/a&gt;</style></title><secondary-title><style face="normal" font="default" size="100%">FEMS Microbiology Letters</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><volume><style face="normal" font="default" size="100%">241</style></volume><pages><style face="normal" font="default" size="100%">41-48</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;i&gt;Bacillus subtilis&lt;/i&gt; synthesizes at least one arabinanase encoded by the &lt;i&gt;abnA&lt;/i&gt; gene that is able to degrade the polysaccharide arabinan. Here, we report the expression in &lt;i&gt;Escherichia coli&lt;/i&gt; of the full-length &lt;i&gt;abnA&lt;/i&gt; coding region with a His6-tag fused to the C-terminus. The recombinant protein was secreted to the periplasmic space and correctly processed by the &lt;i&gt;E. coli&lt;/i&gt; signal peptidase. The substrate specificity of purified AbnA, the physico-chemical properties and kinetic parameters were determined. Functional analysis studies revealed Glu 215 as a key residue for AbnA hydrolytic activity and indicated that in addition to AbnA &lt;i&gt;B. subtilis&lt;/i&gt; secretes other enzyme(s) able to degrade linear 1,5-α-L-arabinan.&lt;/p&gt;
</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;n/a&lt;/p&gt;
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