@article {REBOCHO201898, title = {Development of a ferrocenyl-based MIP in supercritical carbon dioxide: Towards an electrochemical sensor for bisphenol A}, journal = {The Journal of Supercritical Fluids}, volume = {135}, year = {2018}, note = {n/a}, pages = {98 - 104}, abstract = {n/a}, keywords = {BPA, Electrochemistry, Ferrocenylmethyl methacrylate, Green solvent, Molecular imprinting}, issn = {0896-8446}, doi = {https://doi.org/10.1016/j.supflu.2018.01.006}, url = {http://www.sciencedirect.com/science/article/pii/S0896844617303418}, author = {S{\'\i}lvia Rebocho and Cordas, Cristina M. and Raquel Viveiros and Casimiro, Teresa} } @article {MARCELO2018125, title = {Development of itaconic acid-based molecular imprinted polymers using supercritical fluid technology for pH-triggered drug delivery}, journal = {International Journal of Pharmaceutics}, volume = {542}, number = {1}, year = {2018}, note = {n/a}, pages = {125 - 131}, abstract = {n/a}, keywords = {Crosslinked polymer, Metronidazole, Molecular imprinting, pH-responsive, Supercritical carbon dioxide}, issn = {0378-5173}, doi = {https://doi.org/10.1016/j.ijpharm.2018.03.010}, url = {http://www.sciencedirect.com/science/article/pii/S037851731830156X}, author = {Gon{\c c}alo Marcelo and In{\^e}s C. Ferreira and Raquel Viveiros and Casimiro, Teresa} } @article {VIVEIROS20171025, title = {Development of a molecularly imprinted polymer for a pharmaceutical impurity in supercritical CO2: Rational design using computational approach}, journal = {Journal of Cleaner Production}, volume = {168}, year = {2017}, note = {n/a}, pages = {1025 - 1031}, abstract = {n/a}, keywords = {Affinity polymers, Computational design, Green chemistry, Molecular imprinting, Molecular modelling, Supercritical carbon dioxide}, issn = {0959-6526}, doi = {https://doi.org/10.1016/j.jclepro.2017.09.026}, url = {http://www.sciencedirect.com/science/article/pii/S0959652617320152}, author = {Viveiros, R. and K. Karim and S.A. Piletsky and Heggie, W. and T. Casimiro} } @article {VIVEIROS2017229, title = {Green approach on the development of lock-and-key polymers for API purification}, journal = {Chemical Engineering Journal}, volume = {308}, year = {2017}, note = {n/a}, pages = {229 - 239}, abstract = {n/a}, keywords = {Affinity purification, Genotoxin removal, Green chemistry, Molecular imprinted polymer, Supercritical carbon dioxide}, issn = {1385-8947}, doi = {https://doi.org/10.1016/j.cej.2016.09.040}, url = {http://www.sciencedirect.com/science/article/pii/S1385894716312761}, author = {Raquel Viveiros and Maria In{\^e}s Lopes and William Heggie and Casimiro, Teresa} } @article {VIVEIROS2017341, title = {Green strategy to produce large core{\textendash}shell affinity beads for gravity-driven API purification processes}, journal = {Journal of Industrial and Engineering Chemistry}, volume = {54}, year = {2017}, note = {n/a}, pages = {341 - 349}, abstract = {n/a}, keywords = {3-(Trimethoxysilyl) propyl methacrylate, Genotoxin removal, Plasma technology functionalization, Supercritical carbon dioxide, Surface imprinting technique}, issn = {1226-086X}, doi = {https://doi.org/10.1016/j.jiec.2017.06.012}, url = {http://www.sciencedirect.com/science/article/pii/S1226086X17302897}, author = {Raquel Viveiros and Francisco M. Dias and Maia, Luisa B. and William Heggie and Casimiro, Teresa} } @article {ESTEVES2016206, title = {Molecularly imprinted polymer strategies for removal of a genotoxic impurity, 4-dimethylaminopyridine, from an active pharmaceutical ingredient post-reaction stream}, journal = {Separation and Purification Technology}, volume = {163}, year = {2016}, note = {n/a}, pages = {206 - 214}, abstract = {n/a}, keywords = {4-Dimethylaminopyridine, Active pharmaceutical ingredient purification, Genotoxic impurity, Molecularly imprinted polymer, Mometasone furoate}, issn = {1383-5866}, doi = {https://doi.org/10.1016/j.seppur.2016.01.053}, url = {http://www.sciencedirect.com/science/article/pii/S138358661630048X}, author = {Teresa Esteves and Raquel Viveiros and Jo{\~a}o Bandarra and William Heggie and Casimiro, Teresa and Ferreira, Frederico Castelo} } @article {C4RA11666F, title = {Integrated desulfurization of diesel by combination of metal-free oxidation and product removal by molecularly imprinted polymers}, journal = {RSC Adv.}, volume = {4}, year = {2014}, note = {n/a}, pages = {54948-54952}, publisher = {The Royal Society of Chemistry}, abstract = {

The production of ultra-low-sulfur diesel is an important worldwide demand. In this work a novel integrated method for desulfurization of diesel is proposed based on the combination of Bronsted acid catalyzed oxidation and the selective removal of the oxidized products using a molecularly imprinted polymer (MIP) produced in supercritical carbon dioxide (scCO2). The biphasic oxidation reaction of dibenzothiophene sulfone (DBT){,} as model substrate{,} and H2O2 as oxidant{,} was optimized by testing different acid catalysts{,} and also different phase transfer catalysts (PTC){,} including two different ionic liquids (ILs) trihexyl(tetradecyl)phosphoniumchloride [P6{,}6{,}6{,}14]Cl and Aliquat[registered sign]. The products of the efficient oxidation of DBT{,} dibenzothiophene sulfoxide (DBTSO) and dibenzothiophene sulfone (DBTSO2){,} were then selectively removed from real diesel using the MIP.

}, doi = {10.1039/C4RA11666F}, url = {http://dx.doi.org/10.1039/C4RA11666F}, author = {Ferreira, Joana P. and Raquel Viveiros and Lourenco, Anita and Mara Soares da Silva and Rosatella, Andreia and Casimiro, Teresa and Afonso, Carlos A. M.} } @article {MARTINS2014226, title = {Isolation, analytical quantification and seasonal variation of labdanolic acid from the Portuguese-grown Cistus ladaniferus}, journal = {Industrial Crops and Products}, volume = {60}, year = {2014}, note = {n/a}, pages = {226 - 232}, abstract = {n/a}, keywords = {Benzylation reaction, Labdanolic acid, Molecular imprinted polymers, Non-imprinted polymers}, issn = {0926-6690}, doi = {https://doi.org/10.1016/j.indcrop.2014.06.012}, url = {http://www.sciencedirect.com/science/article/pii/S0926669014003458}, author = {Andr{\'e} N.C. Martins and Svilen P. Simeonov and Lu{\'\i}s M.T. Frija and Raquel Viveiros and Louren{\c c}o, Anita and Mara Soares da Silva and Casimiro, Teresa and Afonso, Carlos A. M.} } @article {Louren{\c c}o2014319, title = {Reborn water-soluble CdTe quantum dots}, journal = {Talanta}, volume = {125}, year = {2014}, note = {n/a}, pages = {319 - 321}, abstract = {

Abstract Recovery of fully aggregated water-soluble CdTe quantum dots was achieved by simple treatment with a strong base. A deprotonation-triggered disaggregation is postulated to be the main mechanism involved in the quantum dots {\textquotedblleft}reborn{\textquotedblright} process.

}, keywords = {Bioimaging}, issn = {0039-9140}, doi = {http://dx.doi.org/10.1016/j.talanta.2014.03.022}, url = {http://www.sciencedirect.com/science/article/pii/S0039914014002033}, author = {Louren{\c c}o, Anita and Casimiro, Teresa and Vasco D.B. Bonif{\'a}cio} } @article {C4RA10179K, title = {Supercritical CO2-assisted synthesis of an ultrasensitive amphibious quantum dot-molecularly imprinted sensor}, journal = {RSC Adv.}, volume = {4}, year = {2014}, note = {n/a}, pages = {63338-63341}, publisher = {The Royal Society of Chemistry}, abstract = {

Molecularly imprinted polymers are simple and robust materials for the selective binding of analytes with affinities and selectivities similar to biological probes. A green supercritical CO2-assisted molecular imprinting protocol enabled the production of smart sensory particles{,} incorporating quantum dots{,} with molecular recognition to bisphenol A at very low concentrations (4 nM). The protocol uses amphibious vinyl-coated quantum dots and enables the design of sensors for a wide range of molecules through a simple{,} low cost and clean technology.

}, doi = {10.1039/C4RA10179K}, url = {http://dx.doi.org/10.1039/C4RA10179K}, author = {Lourenco, A. and Viveiros, R. and Mouro, A. and J. C. Lima and Bonifacio, V. D. B. and T. Casimiro} } @article {SoaresdaSilva201294, title = {Supercritical CO2-assisted preparation of a \{PMMA\} composite membrane for bisphenol A recognition in aqueous environment}, journal = {Chemical Engineering Science}, volume = {68}, number = {1}, year = {2012}, note = {n/a}, pages = {94 - 100}, abstract = {

This work reports a novel strategy to prepare affinity composite membranes using supercritical fluid technology. By blending molecularly imprinted polymeric particles with PMMA, a porous hybrid structure with affinity to the template molecule, bisphenol A, was prepared using a supercritical carbon dioxide (scCO2)-assisted method. Membranes were characterized in terms of morphology, mechanical performance and transport properties. The ability of the polymers and hybrid membranes to adsorb bisphenol A was tested in aqueous solutions and fitted to a linearized Langmuir equation, showing that adsorption takes place at homogeneous affinity binding sites within the imprinted surface. Filtration experiments showed that the imprinted hybrid membrane was able to adsorb higher amounts of template even in non-equilibrium dynamic binding conditions. The hybridization of the \{PMMA\} membrane herein reported conveys two important improvements over neat \{PMMA\} membrane: it introduced molecular affinity towards the template molecule and significantly increased the permeability of the porous structures, which are key parameters in processes that involve membranes. This technique could expand the applications of polymeric beads powders and enhance the efficiency of the membrane{\textquoteright}s transport properties. Our work presents a new method to confer affinity to a porous structure by immobilization of imprinted polymers, combining polymer synthesis and membrane formation using supercritical fluid technology.

}, keywords = {Adsorption}, issn = {0009-2509}, doi = {http://dx.doi.org/10.1016/j.ces.2011.09.015}, url = {http://www.sciencedirect.com/science/article/pii/S0009250911006488}, author = {Mara Soares da Silva and Raquel Viveiros and M{\'o}nica B. Coelho and Aguiar-Ricardo, Ana and Casimiro, Teresa} } @article {C2RA20426F, title = {Supercritical fluid technology as a new strategy for the development of semi-covalent molecularly imprinted materials}, journal = {RSC Adv.}, volume = {2}, year = {2012}, note = {n/a}, pages = {5075-5079}, publisher = {The Royal Society of Chemistry}, abstract = {

Molecularly imprinted polymeric particles with molecular recognition towards Bisphenol A (BPA) were synthesized for the first time using the semi-covalent imprinting approach in supercritical carbon dioxide (scCO2). The material{{\textquoteright}}s affinity to BPA was achieved by co-polymerizing ethylene glycol dimethacrylate (EGDMA) with a template-containing monomer{,} Bisphenol A dimethacrylate (BPADM) in scCO2. Bisphenol A is then cleaved from the polymeric matrix by hydrolysis with tetrabutylammonium hydroxide (n-Bu4OH) also in a supercritical environment{,} taking advantage of the high diffusivity of scCO2. The selectivity of the molecular imprinted polymer (MIP) was assessed by evaluating its capability to bind BPA in comparison with progesterone and [small alpha]-ethinylestradiol. In addition{,} the cross-linked particles were used to prepare a PMMA-based hybrid imprinted membrane by a scCO2-assisted phase inversion method. Results show that the incorporation of MIP particles was able to confer molecular affinity to BPA to the membrane and that at dynamic conditions of filtration{,} this imprinted porous structure was able to adsorb a higher amount of BPA than the corresponding non-imprinted hybrid membrane. Our work represents a valuable greener alternative to conventional methods{,} for the synthesis of affinity materials which are able to maintain molecular recognition properties in water.

}, doi = {10.1039/C2RA20426F}, url = {http://dx.doi.org/10.1039/C2RA20426F}, author = {Mara Soares da Silva and Raquel Viveiros and Aguiar-Ricardo, Ana and Bonifacio, Vasco D. B. and Casimiro, Teresa} } @article {daSilva201161, title = {Development of 2-(dimethylamino)ethyl methacrylate-based molecular recognition devices for controlled drug delivery using supercritical fluid technology}, journal = {International Journal of Pharmaceutics}, volume = {416}, number = {1}, year = {2011}, note = {n/a}, pages = {61 - 68}, abstract = {

This work reports the development of a novel potential body-friendly oral drug delivery system, which consists of a biocompatible molecularly imprinted polymer (MIP), with pH sensitive character and low cross-linking degree (20.2\&$\#$xa0;wt\%), synthesized and processed in supercritical carbon dioxide. The \{MIP\} is synthesized using 2-(dimethylamino)ethyl methacrylate (DMAEMA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, and ibuprofen as molecular recognition template. The imprinted matrix was able to show a higher affinity towards ibuprofen than its corresponding non-imprinted polymer (NIP) meaning that the molecular imprinting in scCO2 was efficient even using a low crosslinking degree. \{MIP\} showed a significant molecular recognition towards the template, presenting higher drug uptake ability in the supercritical impregnation step, loading 33.1\&$\#$xa0;wt\% of ibuprofen compared to only 10.2\&$\#$xa0;wt\% for the \{NIP\} polymer. In vitro drug release experiments, simulating an oral administration, showed different release profiles at pH 2.2 and pH 7.4. Zeta potential measurements were performed to both \{MIP\} and \{NIP\} showing that the imprinting process has a significant influence on the charge of the polymeric particles. Cytotoxicity assays performed with human colorectal carcinoma-derived Caco-2 cells demonstrated that the polymers are biocompatible and could be potentially used in drug delivery applications.

}, keywords = {\{DMAEMA\}}, issn = {0378-5173}, doi = {http://dx.doi.org/10.1016/j.ijpharm.2011.06.004}, url = {http://www.sciencedirect.com/science/article/pii/S0378517311005357}, author = {Mara Soares da Silva and Raquel Viveiros and Patr{\'\i}cia I. Morgado and Aguiar-Ricardo, Ana and Correia, Il{\'\i}dio J and Casimiro, Teresa} } @article {daSilva2011150, title = {Development of molecularly imprinted co-polymeric devices for controlled delivery of flufenamic acid using supercritical fluid technology}, journal = {The Journal of Supercritical Fluids}, volume = {58}, number = {1}, year = {2011}, note = {n/a}, pages = {150 - 157}, abstract = {

This work reports the development of a novel class of affinity co-polymeric materials using supercritical fluid technology. Polymeric materials with molecular recognition to flufenamic acid, were first synthesized in supercritical carbon dioxide (scCO2) using the drug as template. Molecularly imprinted co-polymers of methacrylic acid (MAA) or N-isopropyl acrylamide (NIPAAm) crosslinked with ethylene glycol dimethacrylate (EGDMA) were synthesized using different crosslinking degrees and template:monomer ratios, at 65\&$\#$xa0;{\textdegree}C and 21\&$\#$xa0;MPa. High-pressure \{NMR\} experiments confirmed that the nature of the interactions between the drug and the functional monomers during the polymerization step are mainly hydrogen bonds. scCO2-assisted impregnation revealed that the imprinted matrices were able to uptake higher amounts of flufenamic acid. This effect was particularly evidenced in the more crosslinked matrices, with P(MAA{\textendash}EGDMA) imprinted copolymers binding up to 101.5\&$\#$xa0;mg drug/g polymer against only 50.5\&$\#$xa0;mg/g in the non-imprinted copolymer. In vitro drug delivery experiments showed that imprinted co-polymers release the drug in a more sustained way than the corresponding non-imprinted matrices. Overall it was shown that supercritical fluid technology is a viable approach for the development of self-assembly molecular recognition polymers with potential application in controlled drug delivery systems.

}, keywords = {Crosslinking}, issn = {0896-8446}, doi = {http://dx.doi.org/10.1016/j.supflu.2011.05.010}, url = {http://www.sciencedirect.com/science/article/pii/S0896844611001872}, author = {Mara Soares da Silva and Franklin L. Nobrega and Aguiar-Ricardo, Ana and Cabrita, Eurico J. and Casimiro, Teresa} } @article {SoaresdaSilva20101742, title = {Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as \{HPLC\} stationary phases}, journal = {Biosensors and Bioelectronics}, volume = {25}, number = {7}, year = {2010}, note = {n/a}, pages = {1742 - 1747}, abstract = {

Molecularly imprinted polymers (MIPs) of poly(ethylene glycol dimethacrylate) and poly(N-isopropylacrylamide-co-ethylene glycol dimethacrylate) were synthesized for the first time in supercritical carbon dioxide (scCO2), using Boc-l-tryptophan as template. Supercritical fluid technology provides a clean and one-step synthetic route for the preparation of affinity polymeric materials with sensing capability for specific molecules. The polymeric materials were tested as stationary \{HPLC\} phases for the enantiomeric separation of l- and d-tryptophan. \{HPLC\} results prove that the synthesized \{MIPs\} are able to recognize the template molecule towards its enantiomer which opens up potential applications in chromatographic chiral separation.

}, keywords = {\{HPLC\}}, issn = {0956-5663}, doi = {http://dx.doi.org/10.1016/j.bios.2009.12.023}, url = {http://www.sciencedirect.com/science/article/pii/S0956566309007015}, author = {Mara Soares da Silva and Eva R. V{\~a}o and Temtem, M{\'a}rcio and Lu{\'\i}s Mafra and Jorge Caldeira and Aguiar-Ricardo, Ana and Casimiro, Teresa} } @article {Duarte2006102, title = {Supercritical fluid polymerisation and impregnation of molecularly imprinted polymers for drug delivery}, journal = {The Journal of Supercritical Fluids}, volume = {39}, number = {1}, year = {2006}, note = {n/a}, pages = {102 - 106}, abstract = {

Herein the preparation of molecularly imprinted polymers (MIPs) using supercritical fluid technology is evaluated. Poly(diethylene glycol dimethacrylate), polyDEGDMA, was synthesised in supercritical carbon dioxide (scCO2) using a carboxylic acid end-capped perfluoropolyether oil as stabiliser. Polymerisations were carried out in the presence of different concentrations of two different template drug molecules, salicylic acid and acetylsalicylic acid. Results suggest that molecular imprinted polymers were successfully prepared by supercritical polymerisation and then impregnated with the template in order to prepare controlled release systems.

}, keywords = {Cross-linking}, issn = {0896-8446}, doi = {http://dx.doi.org/10.1016/j.supflu.2006.01.013}, url = {http://www.sciencedirect.com/science/article/pii/S0896844606000362}, author = {Ana Rita C. Duarte and Casimiro, Teresa and Aguiar-Ricardo, Ana and Ana Lu{\'\i}sa Simpl{\'\i}cio and Duarte, Catarina M. M.} }