<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Travelling Beneath the Gold Surface – Part I: study and characterization of laboratory reconstructions of Portuguese seventeenth and eighteenth centuries ground and bole layers</style></title><secondary-title><style face="normal" font="default" size="100%">e.c-nservation Journal</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><urls><related-urls><url><style face="normal" font="default" size="100%">https://sites.fct.unl.pt/sites/default/files/gilt-teller/files/e-consjournal2_sandu_et_al.pdf</style></url></related-urls></urls></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Complementary cross-section based protocol of investigation of polychrome samples of a 16th century Moravian Sculpture by optical, vibrational and mass spectrometric techniques</style></title><secondary-title><style face="normal" font="default" size="100%">Microchemical Journal</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><related-urls><url><style face="normal" font="default" size="100%">https://sites.fct.unl.pt/sites/default/files/gilt-teller/files/microchemical_journal_2013.pdf</style></url></related-urls></urls></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">An innovative, interdisciplinary, and multi-technique study of gilding and painting techniques in the decoration of the Main Altarpiece of Miranda do Douro Cathedral (XVII-XVIIIth centuries, Portugal), </style></title><secondary-title><style face="normal" font="default" size="100%">Microscopy Research and Technique</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><urls><related-urls><url><style face="normal" font="default" size="100%">https://sites.fct.unl.pt/sites/default/files/gilt-teller/files/mrt_md__online.pdf</style></url></related-urls></urls></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">I.C.A. Sandu, S. Schäfer, D. Magrini, S. Bracci &amp; C. A. Roque</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cross-section and staining-based techniques for investigating organic materials in painted and polychrome works of art: a review</style></title><secondary-title><style face="normal" font="default" size="100%">Microscopy and Microanalysis</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://journals.cambridge.org/abstract_S1431927612000554</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">18</style></volume><pages><style face="normal" font="default" size="100%">860-875</style></pages></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sandu I.C.A, Joosten I., Leal N.</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Akira Tanaka &amp; Botan Nakamura</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Optical Imaging Applications for the Study of Cultural Heritage Artifacts</style></title><secondary-title><style face="normal" font="default" size="100%">Optical imaging: Technology, Methods and Applications</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.novapublishers.com/catalog/product_info.php?products_id=33912</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Nova Science Publisher Inc.</style></publisher><pages><style face="normal" font="default" size="100%">65 -108</style></pages></record><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">S. Kuckova</style></author><author><style face="normal" font="default" size="100%">et al</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Protein identification and localization using mass spectrometry and staining tests in cross-sections of polychrome samples</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Cultural Heritage</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.em-consulte.com/article/707476/protein-identification-and-localization-using-mass</style></url></web-urls></urls><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The identification and localization of the proteinaceous binders are essential issues in studies of painting materials and techniques, for further proposing valid restoration and conservation treatments of the painted or polychrome works of art. The challenge for analytical chemists and conservation scientists is the availability of methods able to simultaneously identify and map the presence of the binders in the multilayered structure of a sample and the possibility to use a very low amount of sample from the studied art object (considering also the criteria of minimum sampling). These methods should be fast, reproducible in different artefacts and in case of mixture of protein-based binders with other non-proteinaceous constituents (oils, resins, waxes, gums etc.) and also economical (both in terms of materials and time consume). In this context, the present paper proposes an innovative protocol of investigation using two complementary techniques – Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI-TOF MS) and staining tests (one visible and one fluorescent stain) assisted by Optical Microscopy (OM) on cross-section of samples – for the simultaneous identification and mapping of protein – and oil-based binders in paint materials. The novelty is based on the use of MALDI-TOF MS on cross-sections of paints together with a fluorescent stain for protein identification and mapping (mainly used in the area of proteomics) complementing the use of a traditional visible stain for oil-based material identification. The protocol was successfully applied on several samples taken from a Czech medieval polychrome sculpture, entitled “The Mourning of Jesus Christ” (16th century) belonging to the Moravian Gallery (Brno).&lt;/p&gt;
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