<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Queda, Fausto</style></author><author><style face="normal" font="default" size="100%">Covas, Gonçalo</style></author><author><style face="normal" font="default" size="100%">Silva, Tomé</style></author><author><style face="normal" font="default" size="100%">Santos, Cátia Almeida</style></author><author><style face="normal" font="default" size="100%">Bronze, Maria R.</style></author><author><style face="normal" font="default" size="100%">Cañada, Francisco Javier</style></author><author><style face="normal" font="default" size="100%">Corvo, Marta C.</style></author><author><style face="normal" font="default" size="100%">Filipe, Sérgio R</style></author><author><style face="normal" font="default" size="100%">Marques, M. Manuel B.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A top-down chemo-enzymatic approach towards N-acetylglucosamine-N-acetylmuramic oligosaccharides: Chitosan as a reliable template</style></title><secondary-title><style face="normal" font="default" size="100%">Carbohydrate Polymers</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0144861719308008</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Elsevier</style></publisher><volume><style face="normal" font="default" size="100%">224</style></volume><pages><style face="normal" font="default" size="100%">115133</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;An unprecedented approach towards oligosaccharides containing N-acetylglucosamine-N-acetylmuramic (NAG-NAM) units was developed. These novel bacterial cell wall surrogates were obtained from chitosan via a top down approach involving both chemical and enzymatic reactions. The chemical modification of chitosan using a molecular clamp based strategy, allowed obtaining N-acetylglucosamine-N-acetylmuramic (NAG-NAM) containing oligomers. Intercalation of NAM residues was confirmed through the analysis of oligosaccharide fragments from enzymatic digestion and it was found that this route affords NAG-NAM containing oligosaccharides in 33% yield. These oligosaccharides mimic the carbohydrate basic skeleton of most bacterial cell surfaces. The oligosaccharides prepared are biologically relevant and will serve as a platform for further molecular recognition studies with different receptors and enzymes of both bacterial cell wall and innate immune system. This strategy combining both chemical modification and enzymatic digestion provides a novel and simple route for an easy access to bacterial cell wall fragments – biologically important targets.&lt;/p&gt;
</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;n/a&lt;/p&gt;
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