<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Carvalho, L. R.</style></author><author><style face="normal" font="default" size="100%">Corvo, Mc</style></author><author><style face="normal" font="default" size="100%">Enugala, R.</style></author><author><style face="normal" font="default" size="100%">M.M. Marques</style></author><author><style face="normal" font="default" size="100%">E. J. Cabrita</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Application of HR-MAS NMR in the solid-phase synthesis of a glycopeptide using Sieber amide resin</style></title><secondary-title><style face="normal" font="default" size="100%">Magn Reson Chem</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Amides/*chemistry Glycopeptides/chemical synthesis/*chemistry Magnetic Resonance Spectroscopy/classification Molecular Structure Resins</style></keyword><keyword><style  face="normal" font="default" size="100%">Synthetic/*chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/20222070</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">4</style></number><volume><style face="normal" font="default" size="100%">48</style></volume><pages><style face="normal" font="default" size="100%">323-30</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The solid-phase synthesis (SPS) of a structurally complex glycopeptide, using Sieber amide resin, was monitored by high resolution magic angle spinning NMR, demonstrating the further application of this technique. A synthetic peptidoglycan derivative, a precursor of a biologically active PGN, known to be involved in the cellular recognition, was prepared by SPS. The synthesis involved the preparation of an N-alloc glucosamine moiety and the synthesis of a simple amino acid sequence L-Ala-D-Glu-L-Lys-D-Ala-D-Ala. Last step consisted the coupling, on solid-phase, of the protected muramyl unit to the peptide chain. Proton spectra with good suppression of the polystyrene signals in swollen resin samples were obtained in DMF-d(7) as a solvent and by using a nonselective 1D TOCSY/DIPSI-2 scheme, thus allowing to follow the SPS without losses of compound and cleavage from the resin. The assignment of the proton spectra of the resin-bound amino acid sequence and of the bound glycopeptide was achieved through the combination of MAS COSY, TOCSY and NOESY.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Journal Article</style></work-type><notes><style face="normal" font="default" size="100%">n/a</style></notes></record></records></xml>