@article {RN45,
	title = {Application of HR-MAS NMR in the solid-phase synthesis of a glycopeptide using Sieber amide resin},
	journal = {Magn Reson Chem},
	volume = {48},
	number = {4},
	year = {2010},
	note = {n/a},
	pages = {323-30},
	type = {Journal Article},
	abstract = {<p>The solid-phase synthesis (SPS) of a structurally complex glycopeptide, using Sieber amide resin, was monitored by high resolution magic angle spinning NMR, demonstrating the further application of this technique. A synthetic peptidoglycan derivative, a precursor of a biologically active PGN, known to be involved in the cellular recognition, was prepared by SPS. The synthesis involved the preparation of an N-alloc glucosamine moiety and the synthesis of a simple amino acid sequence L-Ala-D-Glu-L-Lys-D-Ala-D-Ala. Last step consisted the coupling, on solid-phase, of the protected muramyl unit to the peptide chain. Proton spectra with good suppression of the polystyrene signals in swollen resin samples were obtained in DMF-d(7) as a solvent and by using a nonselective 1D TOCSY/DIPSI-2 scheme, thus allowing to follow the SPS without losses of compound and cleavage from the resin. The assignment of the proton spectra of the resin-bound amino acid sequence and of the bound glycopeptide was achieved through the combination of MAS COSY, TOCSY and NOESY.</p>
},
	keywords = {Amides/*chemistry Glycopeptides/chemical synthesis/*chemistry Magnetic Resonance Spectroscopy/classification Molecular Structure Resins, Synthetic/*chemistry},
	issn = {1097-458X (Electronic) 0749-1581 (Linking)},
	doi = {10.1002/mrc.2583},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/20222070},
	author = {Carvalho, L. R. and Corvo, Mc and Enugala, R. and M.M. Marques and E. J. Cabrita}
}
