@article {16163, title = {Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles}, journal = {Biomed Pharmacother}, volume = {101}, year = {2018}, note = {

Ribeiro, A P CAnbu, S
Alegria, E C B A
Fernandes, A R
Baptista, P V
Mendes, R
Matias, A S
Mendes, M
Guedes da Silva, M F C
Pombeiro, A J L
eng
France
Biomed Pharmacother. 2018 May;101:137-144. doi: 10.1016/j.biopha.2018.02.069. Epub 2018 Feb 23.

}, month = {2018}, pages = {137-144}, abstract = {

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5+/-0.1muM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0+/-0.1muM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

}, keywords = {Animals, BSA interaction, Cancer cell lines, Cattle, Cell Survival/*drug effects/physiology, cytotoxicity, DNA interaction, DNA-Binding Proteins/*metabolism, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical/methods, Green chemistry, Green Chemistry Technology/*methods, HCT116 Cells, Humans, Metal Nanoparticles/*toxicity, Plant Extracts/isolation \& purification/metabolism/*toxicity, Protein Binding/drug effects/physiology, Serum Albumin, Bovine/metabolism, Silver nanoparticles (AgNPs), Silver/metabolism/*toxicity, Tea/metabolism/toxicity}, isbn = {1950-6007 (Electronic)0753-3322 (Linking) }, url = {https://www.ncbi.nlm.nih.gov/pubmed/29482059}, author = {Ribeiro, A. P. C. and Anbu, S. and Alegria, E.C.B.A. and Fernandes, A. R. and P.V. Baptista and Mendes, R. and Matias, A.S. and Mendes, M. and Guedes da Silva, M.F.C. and Pombeiro, A. J. L.} }