@article {16156, title = {GLUT1 and GLUT3 involvement in anthocyanin gastric transport- Nanobased targeted approach}, journal = {Sci Rep}, volume = {9}, year = {2019}, note = {

Oliveira, HelderRoma-Rodrigues, Catarina
Santos, Ana
Veigas, Bruno
Bras, Natercia
Faria, Ana
Calhau, Conceicao
de Freitas, Victor
Baptista, Pedro V
Mateus, Nuno
Fernandes, Alexandra R
Fernandes, Iva
eng
Research Support, Non-U.S. Gov{\textquoteright}t
England
Sci Rep. 2019 Jan 28;9(1):789. doi: 10.1038/s41598-018-37283-2.

}, month = {2019}, pages = {789}, abstract = {

Anthocyanins may protect against a myriad of human diseases. However few studies have been conducted to evaluate their bioavailability so their absorption mechanism remains unclear. This study aimed to evaluate the role of two glucose transporters (GLUT1 and GLUT3) in anthocyanins absorption in the human gastric epithelial cells (MKN-28) by using gold nanoparticles to silence these transporters. Anthocyanins were purified from purple fleshed sweet potatoes and grape skin. Silencing of GLUT1 and/or GLUT3 mRNA was performed by adding AuNP@GLUT1 and/or AuNP@GLUT3 to MKN-28 cells. Downregulation of mRNA expression occurred concomitantly with the reduction in protein expression. Malvidin-3-O-glucoside (Mv3glc) transport was reduced in the presence of either AuNP@GLUT1 and AuNP@GLUT3, and when both transporters were blocked simultaneously. Peonidin-3-(6{\textquoteright}-hydroxybenzoyl)-sophoroside-5-glucoside (Pn3HBsoph5glc) and Peonidin-3-(6{\textquoteright}-hydroxybenzoyl-6{\textquoteright}{\textquoteright}-caffeoyl)-sophoroside-5-glucoside (Pn3HBCsoph5glc) were assayed to verify the effect of the sugar moiety esterification at glucose B in transporter binding. Both pigments were transported with a lower transport efficiency compared to Mv3glc, probably due to steric hindrance of the more complex structures. Interestingly, for Pn3HBCsoph5glc although the only free glucose is at C5 and the inhibitory effect of the nanoparticles was also observed, reinforcing the importance of glucose on the transport regardless of its position or substitution pattern. The results support the involvement of GLUT1 and GLUT3 in the gastric absorption of anthocyanins.

}, keywords = {Anthocyanins/chemistry/pharmacokinetics/*pharmacology, Biological Transport, Cell Line, Epithelial Cells/cytology/metabolism, Gastric Absorption, Gastric Mucosa/*cytology/metabolism, Glucose Transporter Type 1/antagonists \& inhibitors/*genetics, Glucose Transporter Type 3/antagonists \& inhibitors/*genetics, Glucosides/chemistry/pharmacokinetics, Gold/*pharmacology, Humans, Ipomoea batatas/chemistry, Metal Nanoparticles, Models, Biological, Vitis/chemistry}, isbn = {2045-2322 (Electronic)2045-2322 (Linking) }, url = {https://www.ncbi.nlm.nih.gov/pubmed/30692585}, author = {Oliveira, H. and Roma-Rodrigues, C. and Santos, A. and B. Veigas and Bras, N. and Faria, A. and Calhau, C. and de Freitas, V. and P.V. Baptista and Mateus, N. and Fernandes, A. R. and Fernandes, I.} }