@article {16149, title = {Occurrence of non-toxic bioemulsifiers during polyhydroxyalkanoate production by Pseudomonas strains valorizing crude glycerol by-product}, journal = {Bioresour Technol}, volume = {281}, year = {2019}, note = {

Kourmentza, ConstantinaAraujo, Diana
Sevrin, Chantal
Roma-Rodriques, Catarina
Lia Ferreira, Joana
Freitas, Filomena
Dionisio, Madalena
Baptista, Pedro V
Fernandes, Alexandra R
Grandfils, Christian
Reis, Maria A M
eng
England
Bioresour Technol. 2019 Jun;281:31-40. doi: 10.1016/j.biortech.2019.02.066. Epub 2019 Feb 15.

}, month = {2019}, pages = {31-40}, abstract = {

While screening for polyhydroxyalkanoate (PHA) producing strains, using glycerol rich by-product as carbon source, it was observed that extracellular polymers were also secreted into the culture broth. The scope of this study was to characterize both intracellular and extracellular polymers, produced by Pseudomonas putida NRRL B-14875 and Pseudomonas chlororaphis DSM 50083, mostly focusing on those novel extracellular polymers. It was found that they fall into the class of bioemulsifiers (BE), as they showed excellent emulsion stability against different hydrocarbons/oils at various pH conditions, temperature and salinity concentrations. Cytotoxicity tests revealed that BE produced by P. chlororaphis inhibited the growth of highly pigmented human melanoma cells (MNT-1) by 50\% at concentrations between 150 and 200mug/mL, while no effect was observed on normal skin primary keratinocytes and melanocytes. This is the first study reporting mcl-PHA production by P. putida NRRL B-14785 and bioemulsifier production from both P. putida and P. chlororaphis strains.

}, keywords = {Bioemulsifiers, Cell Line, Tumor, Cell Proliferation/drug effects, Crude glycerol, cytotoxicity, Glycerol/*metabolism, Humans, Melanoma/pathology, Polyhydroxyalkanoates, Polyhydroxyalkanoates/*biosynthesis, Pseudomonas chlororaphis, Pseudomonas putida, Pseudomonas/*metabolism}, isbn = {1873-2976 (Electronic)0960-8524 (Linking) }, url = {https://www.ncbi.nlm.nih.gov/pubmed/30798087}, author = {C. Kourmentza and D. Araujo and Sevrin, C. and C. Roma-Rodriques and Lia Ferreira, J. and Freitas, F and Dionisio, M. and P.V. Baptista and Fernandes, A. R. and C. Grandfils and Reis, M. A. M.} }