@article {11510,
	title = {Gold nanobeacons for tracking gene silencing in Zebrafish},
	journal = {Nanomaterials},
	year = {2017},
	note = {n/a},
	abstract = {<p>The use of gold nanoparticles for effective gene silencing has demonstrated its potential as a tool for gene expression experiments and for the treatment of several diseases. Here, we used a gold nanobeacon designed to specifically silence the enhanced green fluorescence protein (EGFP) mRNA in embryos of a fli-EGFP transgenic zebrafish line, while simultaneously allowing the tracking and localization of the silencing events via the beacon{\textquoteright}s emission. Fluorescence imaging measurements demonstrated a decrease of the EGFP emission with a concomitant increase in the fluorescence of the Au-nanobeacon. Furthermore, microinjection of the Au-nanobeacon led to a negligible difference in mortality and malformations in comparison to the free oligonucleotide, indicating that this system is a biocompatible platform for the administration of gene silencing moieties. Together, these data illustrate the potential of Au-nanobeacons as tools for in vivo zebrafish gene modulation with low toxicity which may be used towards any gene of interest.</p>
},
	url = {http://www.mdpi.com/2079-4991/7/1/10},
	author = {Cordeiro, Milton and Lara Carvalho and Joana Silva and Leonor Sa{\'u}de and Alexandra R. Fernandes and Pedro V. Baptista.}
}
