@article {11005,
	title = {Sialic acid removal from dendritic cells improves antigen cross-presentation and boosts anti-tumor immune responses},
	journal = {Oncotarget },
	year = {2016},
	abstract = {<p>Dendritic cells (DCs) hold promise for anti-cancer immunotherapy. However, clinically, their efficiency is limited and novel strategies to improve DC-mediated anti-tumor responses are needed. Human DCs display high content of sialic acids, which inhibits their maturation and co-stimulation capacity. Here, we aimed to understand whether exogenous desialylation of DCs improves their anti-tumor immunity. Compared to fully sialylated DCs, desialylated human DCs loaded with tumor-antigens showed enhanced ability to induce autologous T cells to proliferate, to secrete Th1 cytokines, and to specifically induce tumor cell apoptosis. Desialylated DCs showed an increased expression of MHC-I and -II, co-stimulatory molecules and an augmented secretion of IL-12. Desialylated HLA-A*02:01 DCs pulsed with gp100 peptides displayed enhanced peptide presentation through MHC-I, resulting in higher activation ofgp100280{\textendash}288 specific CD8+ cytotoxic T cells. Desialylated murine DCs also exhibited increased MHC and co-stimulatory molecules and higher antigen cross-presentation via MHC-I. These DCs showed higher ability to activate antigen-specific CD4+ and CD8+ T cells, and to specifically induce tumor cell apoptosis. Collectively, our data demonstrates that desialylation improves DCs{\textquoteright} ability to elicit T cell-mediated anti-tumor activity, due to increased MHC-I expression and higher antigen presentation via MHC-I. Sialidase treatment of DCs may represent a technology to improve the efficacy of antigen loaded-DC-based vaccines for anti-cancer immunotherapy.</p>
},
	url = {http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget\&page=article\&op=view\&path[]=9419\&pubmed-linkout=1},
	author = {Mariana Silva and Z{\'e}lia Silva and Gra{\c c}a Marques and Tiago Ferro and M{\'a}rcia Gon{\c c}alves and Mauro Monteiro and Sandra J. van Vliet and Elodie Mohr and Andreia C. Lino and Alexandra R. Fernandes and Fl{\'a}via A. Lima and Yvette van Kooyk and Teresa Matos and Carlos E. Tadokoro and Paula A. Videira}
}
