@article {10.1042/BCJ20240423,
	title = {Tweaking the redox properties of PpcA from Geobacter metallireducens with protein engineering},
	journal = {Biochemical Journal},
	volume = {481},
	year = {2024},
	note = {<p>n/a</p>
},
	month = {12},
	pages = {2017-2036},
	abstract = {<p>Geobacter{\textquoteright}s unique ability to perform extracellular electron transfer (EET) to electrodes in Microbial Fuel Cells (MFCs) has sparked the implementation of sustainable production of electrical energy. However, the electrochemical performance of Geobacter{\textquoteright}s biofilms in MFCs remains challenging to implement industrially. Multiple approaches are being investigated to enhance MFC technologies. Protein engineering of multihaem cytochromes, key components of Geobacter{\textquoteright}s EET pathways, can, conceivably, be pursued to improve the EET chain. The periplasmic cytochrome PpcA bridges ET from the inner to the outer membrane and its deletion impairs this crucial step. The functional characterisation of PpcA homologs from G. sulfurreducens (Gs) and G. metallireducens (Gm) revealed a significantly different redox behaviour even though they only differ by thirteen amino acids. In a previous study, we found that the single replacement of a tryptophan residue by methionine (W45M) in PpcAGm shifted the reduction potential value 33\% towards that of PpcAGs. In this work, we expanded our investigation to include other non-conserved residues by conducting five mutation rounds. We identified the most relevant residues controlling the redox properties of PpcAGm. With just four mutations (K19, G25, N26, W45) the reduction potential value of PpcAGm was shifted 71\% toward that of PpcAGs. Additionally, in the quadruple mutant, it was possible to replicate the haem oxidation order and the functional mechanisms of PpcAGs, which differ from those in PpcAGm. Overall, the mutants exhibit diverse redox and functional mechanisms that could be explored as a library for the future design of minimal, synthetic, ET chains in Geobacter.</p>
},
	issn = {0264-6021},
	doi = {10.1042/BCJ20240423},
	url = {https://doi.org/10.1042/BCJ20240423},
	author = {Portela, Pilar C. and Silva, , Marta A. and Almeida, Alexandre and Damas, Gon{\c c}alo F and Salgueiro, , Carlos A.}
}
