@article {https://doi.org/10.1002/pro.5082,
	title = {Periplasmic electron transfer network in Geobacter sulfurreducens revealed by biomolecular interaction studies},
	journal = {Protein Science},
	volume = {33},
	number = {7},
	year = {2024},
	note = {n/a},
	pages = {e5082},
	abstract = {<p>Abstract Multiheme cytochromes located in different compartments are crucial for extracellular electron transfer in the bacterium Geobacter sulfurreducens to drive important environmental processes and biotechnological applications. Recent studies have unveiled that for particular sets of electron terminal acceptors, discrete respiratory pathways selectively recruit specific cytochromes from both the inner and outer membranes. However, such specificity was not observed for the abundant periplasmic cytochromes, namely the triheme cytochrome family PpcA-E. In this work, the distinctive NMR spectroscopic signatures of these proteins in different redox states were explored to monitor pairwise interactions and electron transfer reactions between each pair of cytochromes. The results showed that the five proteins interact transiently and can exchange electrons between each other revealing intra-promiscuity within the members of this family. This discovery is discussed in the light of the establishment of an effective electron transfer network by this pool of cytochromes. This network is advantageous to the bacteria as it enables the maintenance of the functional working potential redox range within the cells.</p>
},
	keywords = {Electron transfer, Geobacter, Multiheme cytochromes, NMR, protein interactions},
	doi = {https://doi.org/10.1002/pro.5082},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.5082},
	author = {Ferreira, Marisa R. and Leonor Morgado and Salgueiro, , Carlos A.}
}
