@article {18837,
	title = {Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE},
	journal = {Nature Communications},
	volume = {15},
	year = {2024},
	note = {<p>n/a</p>
},
	month = {2024},
	pages = {2434},
	abstract = {<p>Extracellular electron transfer (EET) via microbial nanowires drives globally-important environmental processes and biotechnological applications for bioenergy, bioremediation, and bioelectronics. Due to highly-redundant and complex EET pathways, it is unclear how microbes wire electrons rapidly (\&gt;106 s-1) from the inner-membrane through outer-surface nanowires directly to an external environment despite a crowded periplasm and slow (\&lt;105 s-1) electron diffusion among periplasmic cytochromes. Here, we show that Geobacter sulfurreducens periplasmic cytochromes PpcABCDE inject electrons directly into OmcS nanowires by binding transiently with differing efficiencies, with the least-abundant cytochrome (PpcC) showing the highest efficiency. Remarkably, this defined nanowire-charging pathway is evolutionarily conserved in phylogenetically-diverse bacteria capable of EET. OmcS~heme reduction potentials are within 200 mV of each other, with a midpoint 82 mV-higher than reported previously. This~could explain efficient EET over micrometres at ultrafast (\&lt;200 fs) rates with negligible energy loss. Engineering this minimal nanowire-charging pathway may yield microbial~chassis with improved performance.</p>
},
	isbn = {2041-1723},
	url = {https://doi.org/10.1038/s41467-024-46192-0},
	author = {Portela, Pilar C. and Shipps, Catharine C. and Shen, Cong and Srikanth, Vishok and Salgueiro, , Carlos A. and Malvankar, Nikhil S.}
}
