@article {10.3389/fmicb.2022.909109,
	title = {Reduction Kinetic of Water Soluble Metal Salts by Geobacter sulfurreducens: Fe2+/Hemes Stabilize and Regulate Electron Flux Rates},
	journal = {Frontiers in Microbiology},
	volume = {13},
	year = {2022},
	note = {<p>n/a</p>
},
	abstract = {<p>Geobacter sulfurreducens is a widely applied microorganism for the reduction of toxic metal salts, as an electron source for bioelectrochemical devices, and as a reagent for the synthesis of nanoparticles. In order to understand the influence of metal salts, and of electron transporting, multiheme c-cytochromes on the electron flux during respiration of G. sulfurreducens, the reduction kinetic of Fe<sup>3+</sup>, Co<sup>3+</sup>, V<sup>5+</sup>, Cr<sup>6+</sup>, and Mn<sup>7+</sup> containing complexes were measured. Starting from the resting phase, each G. sulfurreducens cell produced an electron flux of 3.7 {\texttimes} 10<sup>5</sup> electrons per second during the respiration process. Reduction rates were within {\textpm} 30\% the same for the 6 different metal salts, and reaction kinetics were of zero order. Decrease of c-cytochrome concentrations by downregulation and mutation demonstrated that c-cytochromes stabilized respiration rates by variation of their redox states. Increasing Fe<sup>2+</sup>/heme levels increased electron flux rates, and induced respiration flexibility. The kinetic effects parallel electrochemical results of G. sulfurreducens biofilms on electrodes, and might help to optimize bioelectrochemical devices.</p>
},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2022.909109},
	url = {https://www.frontiersin.org/article/10.3389/fmicb.2022.909109},
	author = {Karamash, Maksym and Stumpe, Michael and Dengjel, J{\"o}rn and Salgueiro, , Carlos A. and Giese, Bernd and Fromm, Katharina M}
}
