@article {16898,
	title = {Fast Prototyping Microfluidics: Integrating Droplet Digital Lamp for Absolute Quantification of Cancer Biomarkers},
	journal = {Sensors},
	volume = {20},
	year = {2020},
	abstract = {<p>Microfluidic (MF) advancements have been leveraged toward the development of state-of-the-art platforms for molecular diagnostics, where isothermal amplification schemes allow for further simplification of DNA detection and quantification protocols. The MF integration with loop-mediated isothermal amplification (LAMP) is today the focus of a new generation of chip-based devices for molecular detection, aiming at fast and automated nucleic acid analysis. Here, we combined MF with droplet digital LAMP (ddLAMP) on an all-in-one device that allows for droplet generation, target amplification, and absolute quantification. This multilayer 3D chip was developed in less than 30 minutes by using a low-cost and extremely adaptable production process that exploits direct laser writing technology in {\textquotedblleft}Shrinky-dinks{\textquotedblright} polystyrene sheets. ddLAMP and target quantification were performed directly on-chip, showing a high correlation between target concentration and positive droplet score. We validated this integrated chip via the amplification of targets ranging from five to 500,000 copies/reaction. Furthermore, on-chip amplification was performed in a 10 {\textmu}L volume, attaining a limit of detection of five copies/{\textmu}L under 60 min. This technology was applied to quantify a cancer biomarker, c-MYC, but it can be further extended to any other disease biomarker.</p>
},
	url = {https://doi.org/10.3390/s20061624},
	author = {Beatriz Oliveira and Bruno Veigas and Alexandra R. Fernandes and Hugo {\'A}guas and Rodrigo Martins and Elvira Fortunato and Pedro Viana Baptista}
}
