Automatic sleep apnea detection and sleep classification using the ECG and the Sp02 signals - Lara Simons (FCT-UNL, 2009)
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MicroECG: an integrated platform for the cardiac arrythmia detection and characterization - Bruno Nascimento (FCT-UNL, 2009)
O desenvolvimento de um pacote de software para lidar facilmente com electrocardiogramas de alta resolução tornou-se importante para pesquisa na área de electrocardiografia. O desenvolvimento de novas técnicas para detecção de potenciais tardios e outros problemas associados a arritmias cardíacas têm sido objecto de estudo ao longo dos anos. No entanto, ainda existe a lacuna de um pacote de software que facilmente permita implementar algumas destas inovadoras técnicas de uma forma integrada, possibilitando avaliar técnicas clássicas como o protocolo de Simson para a detecção de sinais não estacionários (potenciais tardios). Algumas destas inovadoras técnicas envolvem a detecção tempo-frequência usando escalogramas ou a análise espectral usando metodologias wavelet-packet, sendo implementadas no software desenvolvido com flexibilidade e versatilidade suficientes para que futuramente sirva de plataforma de pesquisa para o refinamento destas mesmas técnicas no que toca ao processamento de sinais de electrocardiogramas de alta resolução. O software aqui desenvolvido foi também desenhado de forma a suportar dois tipos de ficheiros diferentes provenientes de outros tantos sistemas de aquisição. Os sistemas suportados são o ActiveTwo da Biosemi e o USBamp da g.tec.
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Detection and quantification system of biological matter constituted by one or more optical sensors and one or more light sources, associated process and related applications - Martins, Rodrigo Ferrao De Paiva; Baptista, Pedro Miguel Ribeiro Viana; Fortunato, Elvira Maria Correia (2009)
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ZA200901612; H01L C I,G01N

{Global restructuring and its effects on occupations: towards a new division of labor?} - Krings, Bettina (Universidade Nova de Lisboa, IET/CICS.NOVA-Interdisciplinary Centre on Social Sciences, Faculty of Science and Technology, 2009)
The following contribution considers whether global restructuring creates new forms of the division of labor. On the basis of empirical data from a comparative project in 14 European countries, the author supports the hypothesis that in addition to the ongoing process of the internationalization of work, there are ‘hidden’ effects at the local level. From the perspective of three occupational clusters, dynamics can be observed which have differing impacts on the occupational groups. Thus, there is a simultaneous process of restructuring and redefining skills, labor processes and the working organization which forms the daily reality of working men and women.
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Keywords: division of labor processes; globalization; occupations; quality of work and life

Cancer nanotechnology - Prospects for cancer diagnostics and therapy - Baptista, Pedro Viana (Current Cancer Therapy Reviews, 2009)
Nanotechnology is a multidisciplinary field that brings together diverse fields of research and development such as engineering, biology, physics and chemistry. Formal definitions of nanotechnology refer to man-made devices, components and structures in the 1-100 nm range in at least one dimension. Advances in nanoscience are having a significant impact on many scientific fields, boosting the development of a variety of important technologies. Nanotechnology offers an unprecedented opportunity to interact with cancer cells in real time at the molecular and cellular scale. Because of their small size, nanoscale devices can readily interact with biomolecules on both the surface of cells and inside of cells. The concerted development of nanoscale devices, structures and components have provided essential breakthroughs in monitoring and fighting cancer at the earliest stages of the cancer process. Nanotechnology offers a wealth of tools that may provide researchers with new and innovative ways to diagnose and treat cancer - new imaging agents; systems for real-time assessments of therapeutic and surgical efficacy; multifunctional, targeted devices capable of bypassing biological barriers to deliver multiple therapeutic agents directly to cancer cells and tissues that play a critical role in cancer growth and metastasis; agents that can monitor predictive molecular changes allowing for preventive action against precancerous cells becoming malignant; minimizing costs for multiplex analysis. Nanotechnology, if properly integrated with conventional cancer research, may provide extraordinary prospects towards better diagnosis and effective therapy.
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Keywords: Cancer; Cancer imaging; Cancer molecular diagnostics; cancer therapy; nanoparticles; Nanosensors; nanotechnology

General FRET-based coding for application in multiplexing methods - Letícia Giestas; Vesselin Petrov; Baptista, Pedro V; Lima, João Carlos (Photochemical & Photobiological Sciences, 2009)
FRET can be used as a strategy to assign different simultaneous events in the same sample but {"}cross-talk{"} problems are a limitation. Here we present a contribution for the better understanding of the {"}cross-talk{"} in FRET experiments that include several pairs in the same sample. Using oligonucleotide probes labeled with fluorescent dyes which can be selectively excited at a specific wavelength, and using target oligonucleotides tagged with a fluorescent dye with specific characteristics that allow only it to emit light upon selective excitation of a specific probe by energy transfer (FRET), we aim to identify the exact probe-target hybridized pair. When using three donors to probe the presence of complementary targets, only 20% of possible donor/acceptor combinations give straightforward signals readily identifiable with the sample composition, while in the remaining cases severe cross-excitation prevents the direct identification of the sample composition. To correctly resolve the samples identity, we developed a theoretical model that enables the unequivocal attribution of a sample composition to a given set of fluorescence signals, in the presence of three donors.
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Camelid nanobodies raised against an integral membrane enzyme, nitric oxide reductase - Conrath, Katja; Pereira, Alice S.; Martins, Carlos E.; Timoteo, Cristina G.; Tavares, Pedro; Spinelli, Silvia; Kinne, Joerg; Flaudrops, Christophe; Cambillau, Christian; Muyldermans, Serge; Moura, Isabel; Moura, Jose J. G.; et al. (PROTEIN SCIENCE, 2009)
Nitric Oxide Reductase (NOR) is an integral membrane protein performing the reduction of NO to N(2)O. NOR is composed of two subunits: the large one (NorB) is a bundle of 12 transmembrane helices (TMH). It contains a b type heme and a binuclear iron site, which is believed to be the catalytic site, comprising a heme b and a non-hemic iron. The small subunit (NorC) harbors a cytochrome c and is attached to the membrane through a unique TMH. With the aim to perform structural and functional studies of NOR, we have immunized dromedaries with NOR and produced several antibody fragments of the heavy chain (VHHs, also known as nanobodies (TM)). These fragments have been used to develop a faster NOR purification procedure, to proceed to crystallization assays and to analyze the electron transfer of electron donors. BIAcore experiments have revealed that up to three VHHs can bind concomitantly to NOR with affinities in the nanomolar range. This is the first example of the use of VHHs with an integral membrane protein. Our results indicate that VHHs are able to recognize with high affinity distinct epitopes on this class of proteins, and can be used as versatile and valuable tool for purification, functional study and crystallization of integral membrane proteins.
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Studies on the molecular recognition between bioactive peptides and angiotensin-converting enzyme - Pina, A. S.; Roque, A. C. A. (Journal of Molecular Recognition, 2009)
High blood pressure or hypertension is a condition affecting many individuals and represents a controllable risk factor for cardiovascular diseases such as coronary heart disease and stroke. A non-pharmacological approach to manage these includes the application of food components with antihypertensive activity. Milk protein-derived peptides have been exploited as natural hypotensive agents, namely the peptides Val-Pro-Pro {(VPP)} and Ile-Pro-Pro {(IPP)}, already commercialized in functional foods as a potential alternative to synthetic drugs. These bioactive peptides inhibit in vitro and in vivo the Angiotensin I-converting enzyme {(ACE)}, a protein with an important role in blood pressure regulation. In this work, we attempted to elucidate the possible mode of interaction between the peptides and ACE, including mechanisms of binding to the cofactor Zn2+, and further contrast this with the known mode of inhibition exerted by synthetic drugs {(Captopril}, Enalaprilat and Lisinopril). The bioactive peptide Ala-Leu-Pro-Met-His-Ile-Arg {(ALPMHIR)}, also known to inhibit the enzyme ACE but with a lower efficiency than VPP and IPP, was utilized in the docking studies for comparison. It was observed that the best docking poses obtained for VPP and IPP were located at the ACE catalytic site with very high resemblance to the drugs mode of interaction, including the coordination with Zn2+. As for ALPMHIR, the best docking poses were located in the narrow ACE channel outside the catalytic site, representing higher affinity energies and fewer resemblances with the interaction established by drugs.
{PMID:} 18816584
Keywords: Catalytic Domain; Crystallography; Humans; Hydrogen Bonding; Milk Proteins; Oligopeptides; Protein Binding; Protein Conformation; Zinc; Angiotensin-Converting Enzyme Inhibitors; Peptidyl-Dipeptidase A; X-Ray

ADISI-An efficient tool for the automatic design of integrated spiral inductors - P Pereira; Fino, H.; Coito, F.; Ventim-Neves, M. (16th IEEE International Conference, 2009)
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Top-down vs. Bottom-up modeling to support climate policy - Comparative analysis for the Portuguese economy. - Patrícia Fortes; Simões, Sofia; Júlia Seixas; Denyse van Regemorter (10th IAEE European Conference. Energy, Policies and Technologies for Sustainable Economies., 2009)

An Alternative Mechanism for Diels-Alder Reactions of Evans Auxiliary Derivatives - Bakalova, Snezhana M.; Duarte, Filipe J. S.; Georgieva, Miglena K.; Cabrita, Eurico J.; Santos, A. Gil (Chemistry-a European Journal, 2009)
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Computational tools for modelling FSW and an improved tool for NDT - Santos, T.; Vilaça, P.; Quintino, L.; dos Santos, J. (Welding in the World, 2009)
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Cited By :2Export Date: 27 February 2015CODEN: WDWRA

Density Functional Study of Proline-Catalyzed Intramolecular Baylis-Hillman Reactions - Duarte, Filipe J. S.; Cabrita, Eurico J.; Frenking, Gernot; Santos, A. Gil (Chemistry-a European Journal, 2009)
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Developments in NDT for detecting imperfections in friction stir welds in aluminium alloys - Santos, T.; Vilaça, P.; Quintino, L. (Rivista Italiana della Saldatura, 2009)
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Export Date: 27 February 2015CODEN: RISAA

The effect of Mn, Fe and Cu ions on potash-glass corrosion - Vilarigues, M.; R.C. da Silva (2009)
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Keywords: Absorption; Corrosion; FTIR measurements; optical microscopy; Optical spectroscopy; Rutherford backscattering; Silicates

Electrochromism of Crystal Violet Lactone in the presence of Fe(III)/Fe(II) redox pair - Pinheiro, C; A. J. Parola; Pina, F; Laia, C. A. T. (Electrochimica Acta, 2009)
Selective interaction between Crystal Violet Lactone and Fe(3+)/Fe(2+) in methanol leads to a reversible ionochromic colour change. This interaction can be controlled electrochemically, in order to achieve reversible colour changes with high contrast between colourless and dark blue solutions. The presented system is proposed as an alternative electrochromic solution. (C) 2009 Elsevier Ltd. All rights reserved.
ISI Document Delivery No.: 477KRTimes Cited: 3 Cited Reference Count: 14 Pinheiro, Carlos Parola, A. J. Pina, F. Laia, Cesar A. T. Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Parola, A. Jorge/0000-0002-1333-9076; Fundacao para a Ciencia e Tecnologia [PTDC/QUI/67786/2006]; FCT-MCTES and YDreams for the PhD [SFRH/BDE/15563/2005] Financial support from Fundacao para a Ciencia e Tecnologia (project PTDC/QUI/67786/2006) is acknowledged. CP acknowledges FCT-MCTES and YDreams for the PhD grant SFRH/BDE/15563/2005. Pergamon-elsevier science ltd Oxford

Flash photolysis and stopped flow studies of the 2'-methoxyflavylium network in aq. acidic and alkaline solution - Petrov, V.; Gomes, R; A. J. Parola; Pina, F (Dyes and Pigments, 2009)
The rate and equilibrium constants of the network of chemical species involving the dye 2'-methoxyflavylium tetrafluoroborate were characterized using stopped flow and flash photolysis in both acidic and alkaline aqueous solution. The trans-chalcone is the thermodynamic stable species in acidic solutions 2 < pH < 7; irradiation of trans-chalcone at low pH leads to the corresponding coloured flavylium cation. The system reverts to its initial state in a few hours, in the dark. The kinetics of the various steps in the system were determined; flash photolysis revealed that the rate determining process of flavylium formation is the ring closure to give the corresponding hemiketal. In alkaline medium the ionized transchalcone is the stable form and clear evidence for the existence of a hemiketal species was obtained. A write-read-erase cycle can be performed with this compound. (c) 2008 Elsevier Ltd. All rights reserved.
ISI Document Delivery No.: 358UUTimes Cited: 9 Cited Reference Count: 20 Petrov, Vesselin Gomes, Raquel Parola, A. Jorge Pina, Fernando Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Parola, A. Jorge/0000-0002-1333-9076; Elsevier sci ltd Oxford

Host-Guest Interactions between Molecular Clips and Multistate Systems Based on Flavylium Salts - Gomes, R; A. J. Parola; Bastkowski, F.; Polkowska, J.; Klarner, F. G. (Journal of the American Chemical Society, 2009)
Flavylium salts contain the basic structure and show a pH-dependent sequence of reactions identical to natural anthocyanins, which are responsible for most of the red and blue colors of flowers and fruits. In this work we investigated the effect of the water-soluble molecular clips C1 and C2 substituted by hydrogen phosphate or sulfate groups on the stability and reactions of the flavylium salts 1-4 by the use of UV-vis absorption, fluorescence, and NMR spectroscopy as well as of the time-resolved pH jump and flash photolysis methods. Clip C1 forms highly stable host-guest complexes with the flavylium salts 1 and 2 and the quinoidal base 3A in methanol. The binding constants were determined by fluorometric titration to be log K = 4.1, 4.7, and 5.6, respectively. Large complexation-induced (1)H NMR shifts of guest signals, Delta delta(max), indicate that in the case of the flavylium salts 1 and 2 the pyrylium ring and in the case of the quinoidal base 3A the o-hydroxyquinone ring are preferentially bound inside the clip cavity. Due to the poor solubility of these host-guest complexes in water, the association constants could be only determined in highly diluted aqueous solution by UV-vis titration experiments for the complex formation of clip C1 with the flavylium salt 3AH(+) at pH = 2 and the quinoidal base 3A at pH = 5.3 to be log K = 4.9 for both complexes. Similar results were obtained for the formation of the complexes of the sulfate-substituted clip C2 with flavylium salt 4AH(+) and its quinoidal base 4A which are slightly better soluble in water (log K = 4.3 and 4.0, respectively). According to the kinetic analysis (performed by using the methods mentioned above) the thermally induced trans-cis chalcone isomerization (4Ct -> 4Cc) and the H(2)O addition to flavylium cation 4AH(+) followed by H(+) elimination leading to hemiketal 4B are both retarded in the presence of clip C2, whereas the photochemically induced trans-cis isomerization (4Ct -> 4Cc) is not affected by clip C2. The results presented here are explained with dominating hydrophobic interactions between the molecular clips and the flavylium guest molecules. The other potential interactions (ion-ion, cation-pi, pi-pi, and CH-pi), which certainly determine the structures of these host-guest complexes to a large extent, seem to be of minor importance for their stability.
ISI Document Delivery No.: 465ZYTimes Cited: 12 Cited Reference Count: 46 Gomes, Raquel Parola, A. Jorge Bastkowski, Frank Polkowska, Jolanta Klaerner, Frank-Gerrit Parola, A. Jorge/F-4048-2010; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Parola, A. Jorge/0000-0002-1333-9076; FCT-MCTES; Deutsche Forschungsgemeinschaft; [SFRH/BD/27282/2006] The Portuguese FCT-MCTES is acknowledged for financial support through REQUIMTE and Ph.D. Grant SFRH/BD/27282/2006 (R.G.). J. C. Lima, J. M. G. Martinho, and A. Fedorov are acknowledged for the time-resolved fluorescence measurements. V. Petrov is acknowledged for the experimental setup for flash photolysis measurements. F. Pina is kindly acknowledged for fruitful discussions. The Essen group thanks the "Deutsche Forschungsgemeinschaft" (DFG) for financial support. Amer chemical soc Washington

Ligand-based nuclear magnetic resonance screening techniques. - Viegas, Aldino; Macedo, Anjos L.; Cabrita, Eurico J. (Methods in molecular biology (Clifton, N.J.), 2009)
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Mechanical and metallurgical characterization of friction stir welding joints of AA6061-T6 with AA6082-T6 - Moreira, P.M.G.P.; Santos, T.; Tavares, S.M.O.; Richter-Trummer, V.; Vilaça, P.; de Castro, P.M.S.T. (Materials and Design, 2009)
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Cited By :55Export Date: 27 February 2015

Microstructural modification and ductility enhancement of surfaces modified by FSP in aluminium alloys - Nascimento, F.; Santos, T.; Vilaça, P.; Miranda, R.M.; Quintino, L. (Materials Science and Engineering A, 2009)
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Cited By :26Export Date: 27 February 2015

Multiresponsive chromogenic systems operated by light and electrical inputs - Pinheiro, C; A. J. Parola; Laia, C. A. T.; Camara, A.; Pina, F (New Journal of Chemistry, 2009)
In the framework of supramolecular chemistry, a three component system constituted by Fe(3+)/Fe(2+), crystal violet lactone (CVL) and a spiropyran (SPI) leads to three coloured states, cyan, magenta and yellow, as well as a transparent state, each independently addressable by light and electrical input. The system profits from fine speciation Fe(3+)/Fe(2+) complexes with CVL and SPI. Redox stimulus operates the metal, leading to different coloured complexes (ionochromism), while light stimulus operates the SPI component (photochromism).
ISI Document Delivery No.: 501LBTimes Cited: 2 Cited Reference Count: 22 Pinheiro, Carlos Parola, A. Jorge Laia, Cesar A. T. Camara, Antonio Pina, Fernando Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010 Parola, A. Jorge/0000-0002-1333-9076 Mctes [ptdc/qui/67786/2006, sfrh/bde/15563/2005] MCTES is acknowledged for financial support through project PTDC/QUI/67786/2006 and for a PhD grant PhD SFRH/BDE/15563/2005 (C. P.). Royal soc chemistry Cambridge

A new NDT system for micro imperfections detection: Application to FSW and FSpW - Santos, T.; Vilaça, P.; dos Santos, J.; Quintino, L. (Welding in the World, 2009)
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Cited By :4Export Date: 27 February 2015CODEN: WDWRA

New non-destructive test technique on metal inspection - Rosado, L.; Santos, T.; Piedade, M.; Ramos, P.M.; Vilaça, P. (19th IMEKO World Congress 2009, 2009)
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Conference code: 94618Cited By :1Export Date: 27 February 2015

Photochemistry of 7-Hydroxy-2-(4-hydroxystyryl)-1-benzopyrylium and Related Compounds - Diniz, A. M.; Gomes, R; A. J. Parola; Laia, C. A. T.; Pina, F (Journal of Physical Chemistry B, 2009)
2-Styryl-1-benzopyrylium derivatives exhibit deeper hues and absorption spectra that are substantially red-shifted when compared with their 2-phenyl-1-benzopyrylium analogues. They follow the same pH and light-dependent network of chemical reactions previously described for 2-phenyl-1-benzopyrylium compounds. In this work, the photochromic properties of 7-hydroxy-2-(4-hydroxystyryl)-1-benzopyrylium chloride are reported. This compound was fully characterized by UV-vis absorption, fluorescence emission, pH jumps, and flash photolysis, and its properties were compared with the analogue 7,4'-dihydroxyflavylium (7-hydroxy-2-(4-hydroxyphenyl)-1- benzopyrylium). The trans-chalcones of both compounds lacking the hydroxyl in position 2 were synthesized and used as model compounds since they exhibit cis-trans isomerization but cannot be involved in the other processes resulting from the ring closure. The transient absorption of two triplets attributed to the chalcones Ct/Ct(-), and a tautomer was detected by nanosecond flash photolysis, independent of the existence of the 2-hydroxyl substituent. The experimental results are compatible with the main formation of cis-chalcone from the singlet state. In the case of the styryl derivatives, the fraction of triplet formed from excitation of Ct is much higher, and the fraction of isomerization is much smaller. For this reason, the photochemistry of 7-hydroxy-2-(4-hydroxystyryl)-1-benzopyrylium in water is much less efficient than that of its parent 7,4'-dihydroxyflavylium; however, in the presence of CTAB micelles, intense red colors can be obtained upon irradiation, confirming the usefulness of this family of compounds as photochromic systems.
ISI Document Delivery No.: 395KETimes Cited: 13 Cited Reference Count: 41 Diniz, Ana M. Gomes, Raquel Parola, A. Jorge Laia, Cesar A. T. Pina, Fernando Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; Diniz, Ana /C-6705-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Parola, A. Jorge/0000-0002-1333-9076; Portuguese FCT-MCTES [SFRH/BD/27282/2006] The Portuguese FCT-MCTES is acknowledged for financial trough REQUIMTE and for a Ph.D. Grant SFRH/BD/27282/2006 (RG). Amer chemical soc Washington

The synthesis and reaction network of 2-styryl-1-benzopyrylium salts: An unexploited class of potential colorants - Gomes, R; Diniz, A. M.; Jesus, A.; A. J. Parola; Pina, F (Dyes and Pigments, 2009)
The syntheses, thermodynamic and kinetic properties of a series of 2-styryl-1-benzopyrylium compounds are reported. This family of compounds was found to follow the same pH- and light-dependent network of chemical reactions previously described for flavylium (2-phenyl-1-benzopyrylim) compounds. However, 2-styryl-1-benzopyrylium compounds exhibit absorption spectra substantially red shifted when compared with flavylium analogues (up to 90 nm). In particular, a photochromic system switching from yellow to light blue based on derivatives of natural anthocyanins is for the first time documented. (C) 2008 Elsevier Ltd. All rights reserved.
ISI Document Delivery No.: 386VVTimes Cited: 11 Cited Reference Count: 28 Gomes, Raquel Diniz, Ana M. Jesus, Alexandre Parola, A. Jorge Pina, Fernando Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; Diniz, Ana /C-6705-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Parola, A. Jorge/0000-0002-1333-9076; FCT-MCTES (Portugal) through FEDER; FOCI [POCI/QUI/57735/2004]; [SFRH/BD/27282/2006] FCT-MCTES (Portugal) through FEDER and FOCI 2010 is acknowledged for financial support under project POCI/QUI/57735/2004 and PhD grant SFRH/BD/27282/2006 (RG). Elsevier sci ltd Oxford

Total lead and its stable isotopes in the digestive gland of Octopus vulgaris as a fingerprint - Raimundo, J.; Vale, C.; Caetano, M.; Cesario, R.; Moura, I. (Aquatic Biology, 2009)
We hypothesised that the isotopic signature of Pb in the digestive gland of the common octopus reflects the organisms' sources of Pb, and investigated whether isotopic Pb ratios are useful in characterising octopus populations. A total of 47 Octopus vulgaris individuals were captured between November 2005 and September 2006 in 2 areas of the Portuguese coast, near Matosinhos (Area A; NW coast) and Olhao (Area B; south coast), and digestive glands were analysed for total Pb and its stable isotopes. The same determinations were performed in 22 samples of surface sediments from the 2 areas. Pb concentrations in the digestive gland of specimens from Area B (2.8 to 13.0 mu g g(-1)) exceeded the values found in Area A (1.3 to 8.3 mu g g(-1)). A similar pattern was found for the isotopic Pb ratios: (206)Pb/(207)Pb was 1.173 to 1.185 for Area A and 1.165 to 1.172 for B; (206)Pb/(208)Pb was 0.476 to 0.487 for Area A and 0.318 to 0.483 for B. The different signatures of the digestive glands are in line with those observed in the surface sediments of the 2 coastal areas (e.g. (206)Pb/(207)Pb was 1.179 to 1.207 for Area A and 1.171 to 1.181 for B). However, the isotopic Pb signature of octopus was less radiogenic than that of sediments. Because octopus has a short life span (up to 24 mo) the signature reflects recent sources of Pb that have a less radiogenic signature. The Pb signature of surface sediments tends to integrate the record of the previous few years or decades, due to the frequent resuspension of the upper layer of coastal sediments. The mixing of sediments deposited during those periods results in higher isotopic Pb ratios (more radiogenic). The consistent differences between the 2 areas, in sediments and octopus, points towards the isotopic Pb signature as a possible useful tool to distinguish octopus populations.
Times Cited: 1

The Savings of Energy Saving: Quantifying interactions between energy supply and demand side policies for Portugal - Simões, Sofia; Cleto, João; Patrícia Fortes; Júlia Seixas (ECEEE - European Council for an Energy Efficient Economy 2009 Summer Study, 2009)

Antibody immobilization on magnetic particles - Roque, A. C. A.; Bispo, S; Pinheiro, A R N; Antunes, J M A; Gonçalves, D; Ferreira, H A (Journal of Molecular Recognition, 2009)
Magnetic particles {(MNPs)} offer attractive possibilities in biotechnology. MNPs can get close to a target biological entity, as their controllable sizes range from a few nanometres up to tens of nanometres, and their surface can be modified to add affinity and specificity towards desired molecules. Additionally, they can be manipulated by an external magnetic field gradient. In this work, the study of ferric oxide {(Fe3O4)} MNPs with different coating agents was conducted, particularly in terms of strategies for antibody attachment at the surfaces (covalent and physical adsorption) and the effects of blocking buffer composition and incubation times on the specific and non-specific interactions observed. The considered biological model system consisted of a coating antibody (goat {IgG)}, bovine serum albumin {(BSA)} as blocking agent, and a complementary antibody labelled with FITC (anti-goat {IgG).} The detection of antibody binding was followed by fluorescence microscopy and the intensity of the signals quantified. The ratio between the mean grey values of negative and positive controls, as well as the maximum intensity attainable in positive controls, were considered in the evaluation of the assays efficiency. The covalent immobilization of the coating antibody was more successful as opposed to protein adsorption. For covalent immobilization, silica-coated MNPs, a 5% (w/v) concentration of BSA in the blocking buffer and incubation times of 1 h produced the best results in terms of assay sensitivity. However, when conducting the assay for incubation periods of 10 min, the fluorescence signal was reduced by 44% but the assay specificity was maintained.
{PMID:} 18702173
Keywords: Animals; Antibodies; Biological Assay; Bovine; Buffers; Cattle; Ferric Compounds; Fluorescence; Goats; Immunoglobulins; Magnetics; Microscopy; Monoclonal; Serum Albumin; Anti-Idiotypic

Architecture for remote laboratories based on REST web services - Ngolo, M.; Palma, L. B.; Coito, F.; Gomes, L.; Costa, A. (E-Learning in Industrial Electronics, 2009. ICELIE’09, 2009)
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Assignment of EC Numbers to Enzymatic Reactions with MOLMAP Reaction Descriptors and Random Forests - D. A. R. S. Latino; J. Aires-de-Sousa (J. Chem. Inf. Model., 2009)

Bio-recognition and detection using liquid crystals - Hussain, A.; Pina, A. S.; Roque, A. C. A. (Biosensors and Bioelectronics, 2009)
Liquid crystals {(LCs)} are used extensively by the electronics industry as display devices. Advances in the understanding of the liquid crystalline phase and the chemistry therein lead to the development of LC exhibiting faster switching speed with greater twist angle. This in turn lead to the emergence of liquid crystal displays, rendering dial-and-needle based displays (such as those used in various meters) and cathode ray tubes obsolete. In this article, we review the history of LC and their emergence as an invaluable material for display devices and the more recent discovery of their use as sensing elements in biosensors. This new application of LC as tools in the development of fast and simple biosensors is envisaged to gain more importance in the foreseeable future.
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Keywords: Biosensor; Detection; Liquid crystal; Lyotropic; Thermotropic

Biocompatible and bioactive gum Arabic coated iron oxide magnetic nanoparticles - Roque, Ana C A; Bicho, A.; Batalha, Iris L; Cardoso, Ana S.; Hussain, Abid (Journal of Biotechnology, 2009)
The surface modification of iron oxide magnetic nanoparticles {(MNPs)} with gum Arabic {(GA)} via adsorption and covalent coupling was studied. The adsorption of GA was assessed during MNP chemical synthesis by the co-precipitation method {(MNP_GA)}, and after MNP synthesis on both bare magnetite and {MNP_GA.} The covalent immobilization of GA at the surface of aldehyde-activated {(MNP_GAAPTES)} or aminated MNPs {(MNP_GAEDC)} was achieved through free terminal amino and carboxylate groups from GA. The presence of GA at the surface of the MNPs was confirmed by FTIR and by the quantification of GA by the bicinchoninic acid test. Results indicated that the maximum of GA coating was obtained for the covalent coupling of GA through its free carboxylate groups {(MNP_GAEDC)}, yielding a maximum of 1.8 g of GA bound/g of dried particles. The hydrodynamic diameter of MNPs modified with GA after synthesis resulted in the lowest values, in opposition to the MNPs co-precipitated with GA which presented the tendency to form larger aggregates of up to 1 μm. The zeta potentials indicate the existence of negatively charged surfaces before and after GA coating. The potential of the GA coated MNPs for further biomolecule attachment was assessed through anchorage of a model antibody to aldehyde-functionalized {MNP_GA} and its subsequent detection with an FITC labeled anti-antibody.
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Keywords: Antibody; Biosensing; Bioseparation; gum arabic; Magnetic nanoparticles

A Cognitive Model for Frequency Signal Classification - Antunes, R.; Coito, F. V. (International Journal of Mathematical, Physical and Engineering Sciences, 2009)
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Comments on ?Modeling fractional stochastic systems as non-random fractional dynamics driven Brownian motions? - Ortigueira, M. (Applied Mathematical Modelling, 2009)
Some results presented in the paper ?Modeling fractional stochastic systems as non-random fractional dynamics driven Brownian motions? ?I. Podlubny, Fractional Differential Equations, Academic Press, San Diego, 1999? are discussed in this paper. The slightly modified Grünwald-Letnikov derivative proposed there is used to deduce some interesting results that are in contradiction with those proposed in the referred paper. Keywords: Fractional calculus; Grünwald-Letnikov derivative; Fractional Brownian motion

Development of a new chitosan hydrogel for wound dressing - Ribeiro, Maximiano P; Espiga, Ana; Silva, Daniela; Baptista, Patricia; Henriques, Joaquim; Ferreira, Catarina; Silva, Jorge Carvalho; Borges, João Paulo; Pires, Eduardo; Chaves, Paula; Correia, Ilídio J (Wound repair and regeneration, 2009)
Wound healing is a complex process involving an integrated response by many different cell types and growth factors in order to achieve rapid restoration of skin architecture and function. The present study evaluated the applicability of a chitosan hydrogel (CH) as a wound dressing. Scanning electron microscopy analysis was used to characterize CH morphology. Fibroblast cells isolated from rat skin were used to assess the cytotoxicity of the hydrogel. CH was able to promote cell adhesion and proliferation. Cell viability studies showed that the hydrogel and its degradation by-products are noncytotoxic. The evaluation of the applicability of CH in the treatment of dermal burns in Wistar rats was performed by induction of full-thickness transcutaneous dermal wounds. Wound healing was monitored through macroscopic and histological analysis. From macroscopic analysis, the wound beds of the animals treated with CH were considerably smaller than those of the controls. Histological analysis revealed lack of a reactive or a granulomatous inflammatory reaction in skin lesions with CH and the absence of pathological abnormalities in the organs obtained by necropsy, which supported the local and systemic histocompatibility of the biomaterial. The present results suggest that this biomaterial may aid the re-establishment of skin architecture.

Electro-optical light scattering shutter using electrospun cellulose-based nano-and microfibers - Almeida, Pedro L; Kundu, Sudarshan; Borges, João Paulo; Godinho, Maria Helena; Figueirinhas, Joao L. (Applied Physics Letters, 2009)
Electrospun cellulose-based nano and microfibers and a nematic liquid crystal are used to assemble an electro-optical (EO) light-scattering device that shows enhanced characteristics when compared to similar devices. Based on the controlled scattering of light in the composite system, the device can achieve light transmission coefficients tunable from 1% up to around 89%. Simulation of the EO behavior indicates that the roughness of the polymer-liquid crystal interface is crucial for the optical performance of the device.

The Empirical Mode Decomposition an Useful Tool for Signal Analysis - Rato, R.; Ortigueira, M.D.; Batista, A.G. (New Signal Processing Research., 2009)
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FILTERS: Fractional vs Integer order - Matos, C.; Batista, A.G.; Ortigueira, M.D. (Symposium on Fractional Signals and Systems, Lisbon?09, 2009)
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Fractional Derivatives and Linear Systems - Ortigueira, M.D. (Mobile Computing Research and Applications, 2009)
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Functional brain mapping of ictal activity in gelastic epilepsy associated with hypothalamic hamartoma: A case report - Alberto Leal; José Monteiro; Mário Forjaz Secca; Constança Jordão (Epilepsia, 2009)

Generalized GL Fractional Derivative and its Laplace and Fourier Transform - Ortigueira, M.D.; Trujillo, J.J. (ASME 2009 International Design Engineering Technical Conferences & Computers and Information in Engineering Conference IDETC?CIE 2009, 2009)
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Highly active microbial phosphoantigen induces rapid yet sustained MEK/Erk- and PI-3K/Akt-mediated signal transduction in anti-tumor human gammadelta T-cells - Correia, Daniel V.; D'Orey, Francisco; Cardoso, Bruno A; Lança, Telma; Grosso, Ana R; DeBarros, Ana; Martins, Leila R; Barata, João T.; Silva-santos, Bruno (PloS one, 2009)
BACKGROUND: The unique responsiveness of Vgamma9Vdelta2 T-cells, the major gammadelta subset of human peripheral blood, to non-peptidic prenyl pyrophosphate antigens constitutes the basis of current gammadelta T-cell-based cancer immunotherapy strategies. However, the molecular mechanisms responsible for phosphoantigen-mediated activation of human gammadelta T-cells remain unclear. In particular, previous reports have described a very slow kinetics of activation of T-cell receptor (TCR)-associated signal transduction pathways by isopentenyl pyrophosphate and bromohydrin pyrophosphate, seemingly incompatible with direct binding of these antigens to the Vgamma9Vdelta2 TCR. Here we have studied the most potent natural phosphoantigen yet identified, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP), produced by Eubacteria and Protozoa, and examined its gammadelta T-cell activation and anti-tumor properties. METHODOLOGY/PRINCIPAL FINDINGS: We have performed a comparative study between HMB-PP and the anti-CD3epsilon monoclonal antibody OKT3, used as a reference inducer of bona fide TCR signaling, and followed multiple cellular and molecular gammadelta T-cell activation events. We show that HMB-PP activates MEK/Erk and PI-3K/Akt pathways as rapidly as OKT3, and induces an almost identical transcriptional profile in Vgamma9(+) T-cells. Moreover, MEK/Erk and PI-3K/Akt activities are indispensable for the cellular effects of HMB-PP, including gammadelta T-cell activation, proliferation and anti-tumor cytotoxicity, which are also abolished upon antibody blockade of the Vgamma9(+) TCR Surprisingly, HMB-PP treatment does not induce down-modulation of surface TCR levels, and thereby sustains gammadelta T-cell activation upon re-stimulation. This ultimately translates in potent human gammadelta T-cell anti-tumor function both in vitro and in vivo upon transplantation of human leukemia cells into lymphopenic mice, CONCLUSIONS/SIGNIFICANCE: The development of efficient cancer immunotherapy strategies critically depends on our capacity to maximize anti-tumor effector T-cell responses. By characterizing the intracellular mechanisms of HMB-PP-mediated activation of the highly cytotoxic Vgamma9(+) T-cell subset, our data strongly support the usage of this microbial antigen in novel cancer clinical trials.

An historical overview of drug discovery - Pina, A. S.; Hussain, A.; Roque, A. C. A. (Ligand-Macromolecule Interactions in Drug Discovery, 2009)
Drug Discovery in modern times straddles three main periods. The first notable period can be traced to the nineteenth century where the basis of drug discovery relied on the serendipity of the medicinal chemists. The second period commenced around the early twentieth century when new drug structures were found, which contributed for a new era of antibiotics discovery. Based on these known structures, and with the development of powerful new techniques such as molecular modelling, combinatorial chemistry, and automated high-throughput screening, rapid advances occurred in drug discovery towards the end of the century. The period also was revolutionized by the emergence of recombinant DNA technology, where it became possible to develop potential drugs target candidates. With all the expansion of new technologies and the onset of the "Omics" revolution in the twenty-first century, the third period has kick-started with an increase in biopharmaceutical drugs approved by FDA/EMEA for therapeutic use.

Host cell transcriptional profiling during malaria liver stage infection reveals a coordinated and sequential set of biological events - Albuquerque, Sónia S; Carret, Céline; Ana Rita Grosso; Tarun, Alice S; Peng, Xinxia; Kappe, Stefan HI I; Miguel Prudêncio; Maria M. Mota (BMC Genomics, 2009)
BACKGROUND: Plasmodium sporozoites migrate to the liver where they traverse several hepatocytes before invading the one inside which they will develop and multiply into thousands of merozoites. Although this constitutes an essential step of malaria infection, the requirements of Plasmodium parasites in liver cells and how they use the host cell for their own survival and development are poorly understood. RESULTS: To gain new insights into the molecular host-parasite interactions that take place during malaria liver infection, we have used high-throughput microarray technology to determine the transcriptional profile of P. berghei-infected hepatoma cells. The data analysis shows differential expression patterns for 1064 host genes starting at 6 h and up to 24 h post infection, with the largest proportion correlating specifically with the early stages of the infection process. A considerable proportion of those genes were also found to be modulated in liver cells collected from P. yoelii-infected mice 24 and 40 h after infection, strengthening the data obtained with the in vitro model and highlighting genes and pathways involved in the host response to rodent Plasmodium parasites. CONCLUSION: Our data reveal that host cell infection by Plasmodium sporozoites leads to a coordinated and sequential set of biological events, ranging from the initial stage of stress response up to the engagement of host metabolic processes and the maintenance of cell viability throughout infection.

How to mimic the shapes of plant tendrils on the nano and microscale: spirals and helices of electrospun liquid crystalline cellulose derivatives - Godinho, Maria Helena; Canejo, João Paulo; Pinto, Luis FV; Borges, João Paulo; Teixeira, Paulo IC (Soft Matter, 2009)
We show that suspended nano and microfibres electrospun from liquid crystalline cellulosic solutions will curl into spirals if they are supported at just one end, or, if they are supported at both ends, will twist into a helix of one handedness over half of its length and of the opposite handedness over the other half, the two halves being connected by a short straight section. This latter phenomenon, known as perversion, is a consequence of the intrinsic curvature of the fibres and of a topological conservation law. Furthermore, agreement between theory and experiment can only be achieved if account is taken of the intrinsic torsion of the fibres. Precisely the same behaviour is known to be exhibited by the tendrils of climbing plants such as Passiflora edulis, albeit on a lengthscale of millimetres, i.e., three to four orders of magnitude larger than in our fibres. This suggests that the same basic, coarse-grained physical model is applicable across a range of lengthscales.

Ligand-Macromolecule Interactions in Drug Discovery - Roque, A. C. A. (Methods in Molecular Biology, Humana Press Inc., 2009)

Molecular Dynamics of Ethylene Glycol Dimethacrylate Glass Former: Influence of Different Crystallization Pathways - M. T. Viciosa; N. T. Correia; M. Salmerón Sánchez; J. L. Gómez Ribelles; M. Dionísio (Journal of Phyiscal Chemsitry B, 2009)

Molybdenum and tungsten enzymes: a crystallographic and mechanistic overview - Romão, Maria João (Dalton Transactions, 2009)

On the Linear Scale Fractional Systems: An Application of the Fractional Quantum Derivative - Ortigueira, M.D. (On the Linear Scale Fractional Systems: An Application of the Fractional Quantum Derivative, 2009)
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The Operation of Autonomous Mobile Robot Assistants in the Environment of Care Facilities Adopting a User-Centered Development Design - Compagna, Diego; Derpmann, Stefan; Mauz, Kathrin (Enterprise and Work Innovation Studies, 2009)
The successful development of autonomous mobile robot assistants depends significantly on the well-balanced reconcilements of the technically possible and the socially desirable. Based on empirical research 2 substantiated conclusions can be established for the suitability of "scenario-based design" (Rosson/Carroll 2003) for the successful development of mobile robot assistants and automated guided vehicles to be applied for service functions in stationary care facilities for seniors.
The participation of the Fraunhofer Institute for Manufacturing Engineering and Automation IPA (Stuttgart, Germany) and the companies User Interface Design GmbH (Ludwigsburg, Germany) plus MLR System GmbH (Ludwigsburg, Germany) enabled the research and findings presented in this paper; we would like to namely mention Birgit Graf and Theo Jacobs (Fraunhofer IPA) furthermore Peter Klein and Christiane Hartmann (User Interface Design GmbH).

Outer membrane cytochrome c, OmcF, from Geobacter sulfurreducens: High structural similarity to an algal cytochrome c6 - Pokkuluri, P.R.; Londer, Y.Y.; Wood, S.J.; Duke, N.E.C.; Morgado, L.; Salgueiro, C.A.; Schiffer, M. (Proteins: Structure, Function, and Bioinformatics, 2009)
No abstract included.

The prominent conformational plasticity of lactoperoxidase: A chemical and pH stability analysis - Barbara Boscolo; Sónia S. Leal; Salgueiro, C.A.; Elena M. Ghibaudi; Gomes, C.M. (Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2009)
Lactoperoxidase (LPO) is a structurally complex and stable mammalian redox enzyme. Here we aim at evaluating the influence of ionic interactions and how these intertwine with the structural dynamics, stability and activity of LPO. In this respect, we have compared LPO guanidinium hydrochloride (GdmCl) and urea denaturation pathways and performed a detailed investigation on the effects of pH on the LPO conformational dynamics and stability. Our experimental findings using far-UV CD, Trp fluorescence emission and ESR spectroscopies clearly indicate that LPO charged-denaturation with GdmCl induced a sharp two-step process versus a three-step unfolding mechanism induced by urea. This differential effect between GdmCl and urea suggests that ionic interactions must play a rather prominent role in the stabilization of LPO. With both denaturants, the protein core was shown to retain activity up to near the respective Cm values. Moreover, a pH titration of LPO evidenced no significant conformational alterations or perturbation of heme activity within the 4 to 11 pH interval. In contrast, alterations of ionic interactions by poising LPO at pH 3, 2 and 12 resulted in a loss of secondary structure, loosening of tertiary contacts and loss of activity, which appear to be associated with the perturbation of the hydrophobic core, as evidenced by ANS binding, as well as disruption of the heme pocket demonstrated by optical and EPR spectroscopies. Overall, LPO is characterised by a high degree of peripheral structural plasticity without perturbation of the core heme moiety. The possible physiological meaning of such features is discussed.

Real-Time Monitoring of Molecular Dynamics of Ethylene Glycol Dimethacrylate Glass Former - M. T. Viciosa; N. Correia; M. Salmerón Sánchez; J. L. Gómez Ribelles; Carvalho, A. L.; Romão, M.J.; M. Dionísio (journal of Physical Chemistry B, 2009)

Synthesis about a collaborative project on “Technology Assessment of Autonomous Systems” - Moniz, António B. (Enterprise and Work Innovation Studies, 2009)
The project started in 2009 with the support of DAAD in Germany and CRUP in Portugal under the “Collaborative German-Portuguese University Actions” programme. One central goal is the further development of a theory of technology assessment applied to robotics and autonomous systems in general that reflects in its methodology the changing conditions of knowledge production in modern societies and the emergence of new robotic technologies and of associated disruptive changes. Relevant topics here are handling broadened future horizons and new clusters of science and technology (medicine, engineering, interfaces, industrial automation, micro-devices, security and safety), as well as new governance structures in policy decision making concerning research and development (R&D).

Synthesis about a collaborative project on “Technology Assessment of Autonomous Systems” - Moniz, António B. (Enterprise and Work Innovation Studies, 2009)
The project started in 2009 with the support of DAAD in Germany and CRUP in Portugal under the “Collaborative German-Portuguese University Actions” programme. One central goal is the further development of a theory of technology assessment applied to robotics and autonomous systems in general that reflects in its methodology the changing conditions of knowledge production in modern societies and the emergence of new robotic technologies and of associated disruptive changes. Relevant topics here are handling broadened future horizons and new clusters of science and technology (medicine, engineering, interfaces, industrial automation, micro-devices, security and safety), as well as new governance structures in policy decision making concerning research and development (R&D).

A systematic study of solution and processing parameters on nanofiber morphology using a new electrospinning apparatus - Célia Henriques; Vidinha, Ricardo; Botequim, David; Borges, João Paulo; Silva, Jorge Carvalho (Journal of nanoscience and nanotechnology, 2009)
We assembled a new electrospinning apparatus and used poly(ethylene oxide) as a model polymer to perform a systematic study on the influence of solution and processing parameters on the morphology of electrospun nanofibers. Solution parameters studied were polymer concentration and molecular mass. The solvent used, 60 wt% water,40 wt% ethanol, was the same throughout the study. Processing parameters analyzed were: solution feed rate, needle tip-collector distance and electrostatic potential difference between the needle and collector. Solution viscosity increased both with polymer concentration and molecular mass. Polymer concentration plays a decisive role on the outcome of the electrospinning process: a low concentration led to the formation of beaded fibers; an intermediate concentration yielded good quality fibers; a high concentration resulted in a bimodal size distribution and at even higher concentration a distributed deposition. Fiber diameter increased with polymer molecular mass and higher molecular masses are associated with a higher frequency of splaying events. Fiber diameter increased linearly with solution feed rate. While an increase in needle-collector distance represents a weaker electric field, a greater distance to be covered by the fibers and a longer flight time, presumably favoring the formation of thinner fibers, as solvent evaporation leads to a local increase of concentration and viscosity, viscoelastic forces opposing stretching caused an increase of fiber diameter with needle-collector distance. A higher voltage applied at the needle is associated with a higher charging of the polymer and a higher electrical current through it ultimately leading to incomplete solvent evaporation and merged fibers being produced. Controlling the charging of the polymer independently of the electric field strength was achieved by applying a voltage to the collector while distance and potential difference were kept constant. The increased electrostatic repulsion associated with an increase of the high voltage applied to the needle led to the disappearance of merged fibers.

The tetraheme cytochrome from Shewanella oneidensis MR-1 shows thermodynamic bias for functional specificity of the hemes - Fonseca, B.M.; Saraiva, I.H.; Paquete, C.M.; Soares, C.M.; Pacheco, I.; Salgueiro, C.A.; Louro, R.O. (Journal of Biological Inorganic Chemistry, 2009)
Bacteria of the genus Shewanella contain an abundant small tetraheme cytochrome in their periplasm when growing anaerobically. Data collected for the protein isolated from S. oneidensis MR-1 and S. frigidimarina indicate differences in the order of oxidation of the hemes. A detailed thermodynamic characterization of the cytochrome from S. oneidensis MR-1 in the physiological pH range was performed, with data collected in the pH range 5.5-9.0 from NMR experiments using partially oxidized samples and from redox titrations followed by visible spectroscopy. These data allow the parsing of the redox and redox-protonation interactions that occur during the titration of hemes. The results show that electrostatic effects dominate the heme-heme interactions, in agreement with modest redox-linked structural modifications, and protonation has a considerable influence on the redox properties of the hemes in the physiological pH range. Theoretical calculations using the oxidized and reduced structures of this protein reveal that the bulk redox-Bohr effect arises from the aggregate fractional titration of several of the heme propionates. This detailed characterization of the thermodynamic properties of the cytochrome shows that only a few of the multiple microscopic redox states that the protein can access are significantly populated at physiological pH. On this basis a functional pathway for the redox activity of the small tetraheme cytochrome from S. oneidensis MR-1 is proposed, where reduction and protonation are thermodynamically coupled in the physiological range. The differences between the small tetraheme cytochromes from the two organisms are discussed in the context of their biological role.

Thermodynamic characterization of the redox centres in a representative domain of a novel c-type multihaem cytochrome - Leonor Morgado; Fernandes, Ana P.; Londer, Yuri Y.; Pokkuluri, P.R.; Schiffer, Marianne; Salgueiro, C.A. (Biochemical Journal, 2009)
Multihaem cytochromes that could form protein “nanowires” were identified in the Geobacter sulfurreducens genome, and represent a new type of multihaem cytochrome. The sequences of these proteins, two with 12 haems (GSU1996, GSU0592) and one with 27 haems (GSU2210), suggest that they are formed with domains homologous to the trihaem cytochrome c7. Although all three haems have bis-His co-ordination in cytochromes c7, in each domain of the above polymers, the haem equivalent to haem IV has His-Met co-ordination. We previously determined the structure and measured the macroscopic redox potential of one representative domain (domain C) of a dodecahaem cytochrome (GSU1996). In the present study, the microscopic redox properties of the individual haem groups of domain C were determined using NMR and UV–visible spectroscopies. The reduction potentials of the haems for the fully reduced and protonated protein are different from each other (haem I, −106 mV; haem III, −136 mV; and haem IV, −125 mV) and are strongly modulated by redox interactions. This result is rather surprising since the His-Met co-ordinated haem IV does not have the highest potential as was expected. The polypeptide environment of each haem group and the strong haem pairwise redox interactions must play a dominant role in controlling the individual haem potentials. The strong redox interactions between the haems extend the range of their operating potentials at physiological pH (haem I, −71 mV, haem III, −146 mV and haem IV, −110 mV). Such a modulation in haem potentials is likely to have a functional significance in the metabolism of G. sulfurreducens.

Tuning of functional heme reduction potentials in Shewanella fumarate reductases - Miguel Pessanha; Emma L. Rothery; Caroline S. Miles; Graeme A. Reid; Stephen K. Chapman; Ricardo O. Louro; Turner, David L.; Salgueiro, C.A.; Xavier, António V. (Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2009)
The fumarate reductases from S. frigidimarina NCIMB400 and S. oneidensis MR-1 are soluble and monomeric enzymes located in the periplasm of these bacteria. These proteins display two redox active domains, one containing four c-type hemes and another containing FAD at the catalytic site. This arrangement of single-electron redox co-factors leading to multiple-electron active sites is widespread in respiratory enzymes. To investigate the properties that allow a chain of single-electron co-factors to sustain the activity of a multi-electron catalytic site, redox titrations followed by NMR and visible spectroscopies were applied to determine the microscopic thermodynamic parameters of the hemes. The results show that the redox behaviour of these fumarate reductases is similar and dominated by a strong interaction between hemes II and III. This interaction facilitates a sequential transfer of two electrons from the heme domain to FAD via heme IV.

ublic Participation in Sustainable Urban Planning - Amado, Miguel; Santos, Catarina; Evelyne Moura; Julia Pinto (Electronic Journal WASET, 2009)

Using discrete-variable optimization for CMOS spiral inductor design - P Pereira; Fino, M.H.; Coito, F. V. (Microelectronics (ICM), 2009 International Conference on. :324–327.: IEEE, 2009)
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Ziegler-Natta catalysed polymerisation for the preparation of potentially biodegradable copolymers with pendant sucrose moieties - M. T. Barros; K. T. Petrova (Eur. Polym J., 2009)

ADISI-An efficient tool for the automatic design of integrated spiral inductors - P Pereira; Fino, H.; Coito, F.; Ventim-Neves, M. (Electronics, Circuits, and Systems, 2009. ICECS 2009. 16th IEEE International Conference on, 2009)
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Amino acid-functionalized cyclopentadienyl molybdenum tricarbonyl complex and its use in catalytic olefin epoxidation - Abrantes, Marta; Paz, Filipe A. Almeida; Valente, Anabela A.; Pereira, Claudia C. L.; Gago, Sandra; Rodrigues, Alirio E.; Klinowski, Jacek; Pillinger, Martyn; Goncalves, Isabel S. (Journal of Organometallic Chemistry, 2009)
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Times Cited: 23 Gago, Sandra/H-5413-2012; Abrantes, Marta/C-5140-2009; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Valente, Anabela/H-5922-2013; REQUIMTE, PHOTO/M-4229-2013; Almeida Paz, Filipe/F-3430-2010; REQUIMTE, LAQV/N-9835-2013 Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Almeida Paz, Filipe/0000-0003-2051-5645; 23

Architecture for remote laboratories based on REST web services - Ngolo, M.; Palma, L. B.; Coito, F.; Gomes, L.; Costa, A. (E-Learning in Industrial Electronics, 2009. ICELIE’09. 3rd IEEE International Conference on, 2009)
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Catalytic Epoxidation and Sulfoxidation Activity of a Dioxomolybdenum(VI) Complex Bearing a Chiral Tetradentate Oxazoline Ligand - Neves, Patricia; Gago, Sandra; Pereira, Claudia C. L.; Figueiredo, Sonia; Lemos, Americo; Lopes, Andre D.; Goncalves, Isabel S.; Pillinger, Martyn; Silva, Carlos M.; Valente, Anabela A. (Catalysis Letters, 2009)
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Times Cited: 26 Neves, Patricia/F-6098-2011; Lopes, Andre/E-2136-2012; Figueiredo, Sonia/E-4355-2012; Gago, Sandra/H-5413-2012; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Valente, Anabela/H-5922-2013; REQUIMTE, PHOTO/M-4229-2013; Lemos, Americo/B-4892-2008; CIQA, CIQA/N-6549-2013; REQUIMTE, LAQV/N-9835-2013 Lopes, Andre/0000-0002-9702-1216; Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Lemos, Americo/0000-0001-9588-4555; 26

Challenges for Europe under value chain restructuring: Contributions to policy debates - Meil, Pamela; Stratigaki, Maria; Linardos, Petros; Tengblad, Per; Docherty, Peter; Bannink, Duco; Moniz, Antonio; Margarida Paulos; Krings, Bettina; Nierling, Linda (2009)
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Keywords: 2000). Thus systems differ in terms of their exclusivity; Cebriàn & Lallement; closure or segmentation. Corporatist systems in which some workers and sectors are well-regulated tend to protect core workers. Here group identity and divisions between labour would be the standard and discourse of the policy context. Liberal systems are; norms and discourse in which policy is made (O´Reilly; so that the discourse surrounds individual ability and attractiveness on the labour market. –; The policy challenges presented here have been derived from the empirical results obtained from the WORKS quantitative and qualitative data. Thus they have been informed by the analysis of survey results and particularly the workplace level analyses on re; we first set out to situate our particular theme in a larger conceptual landscape and address the following issues: - What current policy debates surround the issue under investigation? - What institutions or structures currently exist that are mainly res; which actors should address the challenges? Policy formulation is refracted through the different social structural frameworks in different countries. Institutional and historical contexts shape these structures. The contexts also shape expectations; which laid the foundation for identifying policy challenges. In the complex and diverse area of policy presented here

Characterization of weld (Reseda luteola L.) and spurge flax (Daphne gnidium L.) by high-performance liquid chromatography-diode array detection-mass spectrometry in Arraiolos historical textiles - Marques, Rita; Sousa, Micaela M.; Oliveira, Maria C.; Melo, Maria J. (Journal of Chromatography A, 2009)
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Times Cited: 19 Melo, Maria Joao/C-8594-2011; Oliveira, Maria Conceicao/H-8263-2012; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014 Melo, Maria Joao/0000-0001-7393-6801; Oliveira, Maria Conceicao/0000-0002-3068-4920; 19

Cobalt-, zinc- and iron-bound forms of adenylate kinase (AK) from the sulfate-reducing bacterium Desulfovibrio gigas: purification, crystallization and preliminary X-ray diffraction analysis - Kladova, A. V.; Gavel, O. Yu; Mukhopaadhyay, A.; Boer, D. R.; Teixeira, S.; Shnyrov, V. L.; Moura, I.; Moura, J. J. G.; Romao, M. J.; Trincao, J.; Bursakov, S. A. (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2009)
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Times Cited: 1 Romao, Maria/A-4115-2013; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Moura, Isabel/0000-0003-0971-4977; 1

A Cognitive Model for Frequency Signal Classification - Antunes, R.; Coito, F. V. (International Journal of Mathematical, Physical and Engineering Sciences, 2009)
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Collective Modes and Biaxial Ordering Observed by Deuterium NMR in the Nematic Phases of an Organosiloxane Tetrapode - J. L. Figueirinhas; C. Cruz; Feio, G.; Mehl, G. H. (Molecular Crystals and Liquid Crystals, 2009)
Calculations of deuterium NMR spectra were performed using a model of slow motions based on the collective modes present in liquid crystalline systems and evaluated within the Landau de Gennes free energy expansion on the order parameter tensor. Simulations obtained with this model are applied to the case of deuterium NMR spectra collected in static and rotating samples of organosiloxane tetrapodes exhibiting uniaxial and biaxial nematic phases. The analysis of the slow motions influence on deuterium NMR spectra shows that molecular motions within a time-scale of the order of magnitude of NMR observation times are particularly effective on modulating the NMR line-shape in the case of the liquid crystalline system investigated.
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Keywords: biaxiality collective modes dendrimers deuterium nmr organosiloxane tetrapodes liquid-crystals

Comments on ?Modeling fractional stochastic systems as non-random fractional dynamics driven Brownian motions? - Ortigueira, M. (Applied Mathematical Modelling, 2009)
Some results presented in the paper ?Modeling fractional stochastic systems as non-random fractional dynamics driven Brownian motions? ?I. Podlubny, Fractional Differential Equations, Academic Press, San Diego, 1999? are discussed in this paper. The slightly modified Grünwald-Letnikov derivative proposed there is used to deduce some interesting results that are in contradiction with those proposed in the referred paper.
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Comments on ?Modeling fractional stochastic systems as non-random fractional dynamics driven Brownian motions? - Ortigueira, M.D. (Applied Mathematical Modelling, 2009)
Some results presented in the paper ?Modeling fractional stochastic systems as non-random fractional dynamics driven Brownian motions? ?I. Podlubny, Fractional Differential Equations, Academic Press, San Diego, 1999? are discussed in this paper. The slightly modified Grünwald-Letnikov derivative proposed there is used to deduce some interesting results that are in contradiction with those proposed in the referred paper. Keywords: Fractional calculus; Grünwald-Letnikov derivative; Fractional Brownian motion
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Comparison of liquid-phase olefin epoxidation catalysed by dichlorobis-(dimethylformamide)dioxomolybdenum(VI) in homogeneous phase and grafted onto MCM-41 - Monteiro, Bernardo; Balula, Salete S.; Gago, Sandra; Grosso, Carla; Figueiredo, Sonia; Lopes, Andre D.; Valente, Anabela A.; Pillinger, Martyn; Lourenco, Joao P.; Goncalves, Isabel S. (Journal of Molecular Catalysis a-Chemical, 2009)
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Times Cited: 22 Lourenco, Joao/A-7758-2008; Balula, Salete/B-7508-2012; Lopes, Andre/E-2136-2012; Figueiredo, Sonia/E-4355-2012; Gago, Sandra/H-5413-2012; Monteiro, Bernardo/C-6940-2013; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Valente, Anabela/H-5922-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, MAT/M-4540-2013; REQUIMTE, NSF/N-1636-2013; CIQA, CIQA/N-6549-2013; REQUIMTE, LAQV/N-9835-2013 Lourenco, Joao/0000-0002-0501-6672; Balula, Salete/0000-0002-8984-0473; Lopes, Andre/0000-0002-9702-1216; Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; 23

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas - Najmudin, Shabir; Bonifacio, Cecilia; Duarte, Americo G.; Pualeta, Sofia R.; Moura, Isabel; Moura, Jose J. G.; Romao, Maria J. (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2009)
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Times Cited: 2 Romao, Maria/A-4115-2013; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Moura, Isabel/0000-0003-0971-4977; 7 2

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas. (vol F65, pg 730, 2009) - Najmudin, Shabir; Bonifacio, Cecilia; Duarte, Americo G.; Pauleta, Sofia R.; Moura, Isabel; Moura, Jose J. G.; Romao, Maria J. (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2009)
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Times Cited: 0 Romao, Maria/A-4115-2013; Pauleta, Sofia/F-3619-2011; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Pauleta, Sofia/0000-0002-2149-9416; Moura, Isabel/0000-0003-0971-4977; 8 0

Deuterium NMR study of mobility and fluctuations in nematic and isotropic elastomers - Feio, G.; J. L. Figueirinhas; Tajbakhsh, A. R.; Terentjev, E. M. (J Chem Phys, 2009)
Orientational ordering in polydomain nematic and isotropic elastomers with identical polysiloxane backbone and different deuterium-labeled side groups is studied by D-NMR. In the nematic elastomer the orientational order parameter grows in a critical fashion on crossing the I-N transition implying a continuous phase transition driven by critical fluctuations of local director. The orientational (nematic) ordering occurs on the background of the polymer dynamics exhibited by the backbone, which is similar in the nematic and the analogous isotropic elastomers. The temperature dependence of NMR linewidths is compatible with a Vogel-Fulcher glassy dynamics.
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Effect of an Ionic Liquid on the Catalytic Performance of Thiocyanatodioxomolybdenum(VI) Complexes for the Oxidation of Cyclooctene and Benzyl Alcohol - Monteiro, Bernardo; Gago, Sandra; Neves, Patricia; Valente, Anabela A.; Goncalves, Isabel S.; Pereira, Claudia C. L.; Silva, Carlos M.; Pillinger, Martyn (Catalysis Letters, 2009)
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Times Cited: 17 Neves, Patricia/F-6098-2011; Gago, Sandra/H-5413-2012; Monteiro, Bernardo/C-6940-2013; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Valente, Anabela/H-5922-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; 17

The Effect of the Sixth Sulfur Ligand in the Catalytic Mechanism of Periplasmic Nitrate Reductase - Cerqueira, N. M. F. S. A.; Gonzalez, P. J.; Brondino, C. D.; Romao, M. J.; Romao, C. C.; Moura, I.; Moura, J. J. G. (Journal of Computational Chemistry, 2009)
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Times Cited: 14 Romao, Carlos /D-1162-2010; Cerqueira, Nuno M. F. Sousa A./A-5182-2008; Romao, Maria/A-4115-2013; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Gonzalez, Pablo/D-8200-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, TCB/M-6190-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Carlos /0000-0001-5061-3743; Cerqueira, Nuno M. F. Sousa A./0000-0003-0342-7424; Romao, Maria/0000-0002-3004-0543; Moura, Isabel/0000-0003-0971-4977; 14

Effect of Water/Carboxymethylcellulose Gel on the Excimer Formation of Polyamine Ligands Functionalized with Naphthalene - Rodriguez, Laura; Delgado-Pinar, Estefania; Sornosa-Ten, Alejandra; Alarcon, Javier; Garcia-Espana, Enrique; Cano, Manoli; Lima, Joao C.; Pina, Fernando (Journal of Physical Chemistry B, 2009)
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The Empirical Mode Decomposition an Useful Tool for Signal Analysis - Raul Rato; Ortigueira, M.D.; Batista, A.G. (New Signal Processing Research, 2009)
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FILTERS: Fractional vs Integer order - Matos, C.; Batista, A.G.; Ortigueira, M.D. (Symposium on Fractional Signals and Systems, Lisbon?09, 2009)
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Fractional Derivatives and Linear Systems - Ortigueira, M.D. (Mobile Computing Research and Applications, 2009)
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General FRET-based coding for application in multiplexing methods - Leticia Giestas; Vesselin Petrov; Pedro V. Baptista; Lima, Joao Carlos (Photochemical & Photobiological Sciences, 2009)
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Generalized GL Fractional Derivative and its Laplace and Fourier Transform - Ortigueira, M.D.; Trujillo, J.J. (ASME 2009 International Design Engineering Technical Conferences & Computers and Information in Engineering Conference IDETC?CIE 2009, 2009)
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Heterometallic complexes involving iron(II) and rhenium(VII) centers connected by mu-oxido bridges - Goncalves, Isabel S.; Lopes, Andre D.; Amarante, Tatiana R.; Almeida Paz, Filipe A.; Silva, Nuno J. O.; Pillinger, Martyn; Gago, Sandra; Palacio, Fernando; Kuehn, Fritz E.; Romao, Carlos C. (Dalton Transactions, 2009)
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Times Cited: 1 Kuhn, Fritz/A-3113-2010; Amarante, Tatiana/C-7816-2011; Silva, Nuno/D-2896-2012; Lopes, Andre/E-2136-2012; Romao, Carlos /D-1162-2010; Gago, Sandra/H-5413-2012; Palacio, Fernando/A-1270-2013; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; REQUIMTE, PHOTO/M-4229-2013; CIQA, CIQA/N-6549-2013; Almeida Paz, Filipe/F-3430-2010; REQUIMTE, LAQV/N-9835-2013 Silva, Nuno/0000-0003-2260-1192; Lopes, Andre/0000-0002-9702-1216; Romao, Carlos /0000-0001-5061-3743; Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Almeida Paz, Filipe/0000-0003-2051-5645; 1

Intrinsically photochromic ionic liquids - Branco, Luis C.; Pina, Fernando (Chemical Communications, 2009)
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Times Cited: 15 Pina, Fernando/C-8161-2011; Branco, Luis/C-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Pina, Fernando/0000-0001-8529-6848; Branco, Luis/0000-0003-2520-1151; 15

Kinetic, Structural, and EPR Studies Reveal That Aldehyde Oxidoreductase from Desulfovibrio gigas Does Not Need a Sulfido Ligand for Catalysis and Give Evidence for a Direct Mo-C Interaction in a Biological System - Santos-Silva, Teresa; Ferroni, Felix; Thapper, Anders; Marangon, Jacopo; Gonzalez, Pablo J.; Rizzi, Alberto C.; Moura, Isabel; Moura, Jose J. G.; Romao, Maria J.; Brondino, Carlos D. (Journal of the American Chemical Society, 2009)
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Times Cited: 12 Thapper, Anders/A-6728-2010; Romao, Maria/A-4115-2013; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Gonzalez, Pablo/D-8200-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Moura, Isabel/0000-0003-0971-4977; 12

Liquid-phase oxidation catalysed by copper(II) immobilised in a pillared layered double hydroxide - Monteiro, Bernardo; Gago, Sandra; Balula, Salete S.; Valente, Anabela A.; Goncalves, Isabel S.; Pillinger, Martyn (Journal of Molecular Catalysis a-Chemical, 2009)
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Times Cited: 12 Balula, Salete/B-7508-2012; Gago, Sandra/H-5413-2012; Monteiro, Bernardo/C-6940-2013; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Valente, Anabela/H-5922-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, MAT/M-4540-2013; REQUIMTE, NSF/N-1636-2013; REQUIMTE, LAQV/N-9835-2013 Balula, Salete/0000-0002-8984-0473; Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; 13

Luminescent and chromogenic molecular probes based on polyamines and related compounds - Lodeiro, Carlos; Pina, Fernando (Coordination Chemistry Reviews, 2009)
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Times Cited: 72 Pina, Fernando/C-8161-2011; Lodeiro, Carlos/B-4793-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, C4O/M-5599-2013; REQUIMTE, UCIBIO/N-9846-2013; REQUIMTE, LAQV/N-9835-2013 Pina, Fernando/0000-0001-8529-6848; Lodeiro, Carlos/0000-0001-5582-5446; 73

Microwave-Assisted Synthesis and Crystal Structure of Oxo(diperoxo)(4,4 '-di-tert-butyl-2,2 '-bipyridine)-molybdenum(VI) - Amarante, Tatiana R.; Almeida Paz, Filipe A.; Gago, Sandra; Goncalves, Isabel S.; Pillinger, Martyn; Rodrigues, Alirio E.; Abrantes, Marta (Molecules, 2009)
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Times Cited: 12 Amarante, Tatiana/C-7816-2011; Gago, Sandra/H-5413-2012; Abrantes, Marta/C-5140-2009; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; REQUIMTE, PHOTO/M-4229-2013; Almeida Paz, Filipe/F-3430-2010; REQUIMTE, LAQV/N-9835-2013 Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Almeida Paz, Filipe/0000-0003-2051-5645; 12

Molybdenum and tungsten enzymes: a crystallographic and mechanistic overview - Romao, Maria Joao (Dalton Transactions, 2009)
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Times Cited: 49 Romao, Maria/A-4115-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; 49

Molybdenum Induces the Expression of a Protein Containing a New Heterometallic Mo-Fe Cluster in Desulfovibrio alaskensis - Rivas, Maria G.; Carepo, Marta S. P.; Mota, Cristiano S.; Korbas, Malgorzata; Durand, Marie-Claire; Lopes, Ana T.; Brondino, Carlos D.; Pereira, Alice S.; George, Graham N.; Dolla, Alain; Moura, Jose J. G.; Moura, Isabel (Biochemistry, 2009)
The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 +/- 13 kDa) consisting of 16-18 monomers of 15321.1 +/- 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 280, 320, and 570 nm with extinction coefficients of 18700, 12800, and 5000 M(-1) cm(-1), respectively. Metal content, EXAFS data and DFT calculations support the presence of a Mo-2S-[2Fe-2S]-2S-Mo cluster never reported before. Analysis of the available genomes from Desulfovibrio species shows that the MorP encoding gene is located downstream of a sensor and a regulator gene. This type of gene arrangement, called two component system, is used by the cell to regulate diverse physiological processes in response to changes in environmemtal conditions. Increase of both gene expression and protein production was observed when cells were cultured in the presence of 45 mu M molybdenum. Involvement of this system in Mo tolerance of sulfate reducing bacteria is proposed.
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More Sustainable Approaches for the Synthesis of N-Based Heterocycles - Candeias, Nuno R.; Branco, Luis C.; Gois, Pedro M. P.; Afonso, Carlos A. M.; Trindade, Alexandre F. (Chemical Reviews, 2009)
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Times Cited: 83 Branco, Luis/C-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; gois, pedro/A-9251-2014; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; gois, pedro/0000-0002-7698-630X; 83

On the Linear Scale Fractional Systems: An Application of the Fractional Quantum Derivative - Ortigueira, M.D. (On the Linear Scale Fractional Systems: An Application of the Fractional Quantum Derivative, 2009)
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On the Mechanism of Photochromism of 4 '-N,N-Dimethylamino-7-hydroxyflavylium in Pluronic F127 - Gomes, Raquel; Laia, Cesar A. T.; Pina, Fernando (Journal of Physical Chemistry B, 2009)
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Times Cited: 4 Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Laia, Cesar/0000-0001-6410-6072; Pina, Fernando/0000-0001-8529-6848; 4

Optical spectroscopy and photochemistry of porphyrins and phthalocyanines - Costa, Silvia M. B.; Andrade, Suzana M.; Togashi, Denisio M.; Paulo, Pedro M. R.; Laia, Cesar A. T.; Viseu, M. Isabel; da Silva, Amelia M. Goncalves (Journal of Porphyrins and Phthalocyanines, 2009)
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Times Cited: 1 Laia, Cesar/C-8651-2011; Andrade, Suzana/H-6060-2012; Costa, Silvia/H-7766-2012; Paulo, Pedro/H-8157-2012; Goncalves da Silva, Amelia/A-5616-2013; REQUIMTE, PHOTO/M-4229-2013; Costa, Silvia/M-8085-2013; REQUIMTE, LAQV/N-9835-2013 Laia, Cesar/0000-0001-6410-6072; Andrade, Suzana/0000-0002-4464-7020; Paulo, Pedro/0000-0002-8001-4810; Goncalves da Silva, Amelia/0000-0002-8481-580X; Costa, Silvia/0000-0002-6978-8903; 1

Photochromism of 7,4 '-Dihydroxyflavylium in an AOT Reversed Micelle System - Vesselin Petrov; Laia, Cesar A. T.; Pina, Fernando (Langmuir, 2009)
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Times Cited: 7 Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Laia, Cesar/0000-0001-6410-6072; Pina, Fernando/0000-0001-8529-6848; 7

Preliminary structural characterization of human SOUL, a haem-binding protein - Freire, Filipe; Romao, Maria Joao; Macedo, Anjos L.; Aveiro, Susana S.; Goodfellow, Brian J.; Carvalho, Ana Luisa (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2009)
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Times Cited: 2 Freire, Filipe/F-6505-2010; Carvalho, Ana Luisa/G-5638-2011; Romao, Maria/A-4115-2013; Macedo, Anjos/D-7216-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Freire, Filipe/0000-0002-5310-0668; Carvalho, Ana Luisa/0000-0002-3824-0240; Romao, Maria/0000-0002-3004-0543; Macedo, Anjos/0000-0002-2613-4838; 2

Real-Time Monitoring of Molecular Dynamics of Ethylene Glycol Dimethacrylate Glass Former - M. T. Viciosa; N. T. Correia; Salmeron Sanchez, M.; Carvalho, A. L.; Romao, M. J.; Gomez Ribelles, J. L.; Dionisio, M. (Journal of Physical Chemistry B, 2009)
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Times Cited: 11 Salmeron-Sanchez, Manuel/E-4680-2010; Carvalho, Ana Luisa/G-5638-2011; Romao, Maria/A-4115-2013; Dionisio, Madalena/D-1946-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, CAT/M-4526-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, NSF/N-1636-2013; REQUIMTE, UCIBIO/N-9846-2013; REQUIMTE, LAQV/N-9835-2013 Salmeron-Sanchez, Manuel/0000-0002-8112-2100; Carvalho, Ana Luisa/0000-0002-3824-0240; Romao, Maria/0000-0002-3004-0543; Dionisio, Madalena/0000-0002-1487-0889; 11

Ring-Functionalized Molybdenocene Complexes - Honzicek, Jan; Mukhopadhyay, Abhik; Santos-Silva, Teresa; Romao, Maria J.; Romao, Carlos C. (Organometallics, 2009)
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Times Cited: 10 Romao, Carlos /D-1162-2010; Romao, Maria/A-4115-2013; Mukhopadhyay, Abhik/D-2119-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Honzicek, Jan/I-6873-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Carlos /0000-0001-5061-3743; Romao, Maria/0000-0002-3004-0543; Mukhopadhyay, Abhik/0000-0002-7755-0401; 10

Solvent effects on the absorption and emission of [Re(R(2)bpy)(CO)(3)X] complexes and their sensitivity to CO2 in solution - Rodriguez, Laura; Ferrer, Montserrat; Rossell, Oriol; Duarte, Filipe J. S.; Santos, A. Gil; Lima, Joao C. (Journal of Photochemistry and Photobiology a-Chemistry, 2009)
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Specific Supramolecular Interactions between Zn2+-Salophen Complexes and Biologically Relevant Anions - Cano, Manoli; Rodriguez, Laura; Lima, Joao Carlos; Pina, Fernando; Dalla Cort, Antonella; Pasquini, Chiara; Schiaffino, Luca (Inorganic Chemistry, 2009)
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Specific Supramolecular Interactions between Zn2+-Salophen Complexes and Biologically Relevant Anions - Cano, Manoli; Rodriguez, Laura; Lima, Joao Carlos; Pina, Fernando; Dalla Cort, Antonella; Pasquini, Chiara; Schiaffino, Luca (Inorganic Chemistry, 2009)
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Times Cited: 30 Pina, Fernando/C-8161-2011; Rodriguez, Laura/A-4169-2011; DALLA CORT, ANTONELLA/F-3587-2012; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; Lima, Joao/F-3658-2010; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Pina, Fernando/0000-0001-8529-6848; Rodriguez, Laura/0000-0003-1289-1587; Lima, Joao/0000-0003-0528-1967; 30

Structural and dynamic characterization of hSOUL, a heme-binding protein - Freire, F.; Macedo, A. L.; Aveiro, S. S.; Romao, M. J.; Carvalho, A. L.; Goodfellow, B. J. (Febs Journal, 2009)
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Times Cited: 0 Freire, Filipe/F-6505-2010; Romao, Maria/A-4115-2013; Macedo, Anjos/D-7216-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; Carvalho, Ana Luisa/G-5638-2011; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Freire, Filipe/0000-0002-5310-0668; Romao, Maria/0000-0002-3004-0543; Macedo, Anjos/0000-0002-2613-4838; Carvalho, Ana Luisa/0000-0002-3824-0240; 34th Congress of the Federation-of-European-Biochemical-Societies Jul 04-09, 2009 Prague, CZECH REPUBLIC Federat European Biochem Soc 0

Studies on dissolution of carbohydrates in ionic liquids and extraction from aqueous phase - Rosatella, Andreia A.; Branco, Luis C.; Afonso, Carlos A. M. (Green Chemistry, 2009)
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Times Cited: 42 Branco, Luis/C-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; Rosatella, Andreia/M-5674-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Rosatella, Andreia/0000-0002-2691-3110; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 43

A study on red lead degradation in a medieval manuscript Lorvao Apocalypse (1189) - Miguel, Catarina; Claro, Ana; Goncalves, Antonio Pereira; Muralha, Vania S. F.; Melo, Maria Joao (Journal of Raman Spectroscopy, 2009)
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Times Cited: 10 Melo, Maria Joao/C-8594-2011; Muralha, Vania/C-7018-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Goncalves, Antonio/J-5954-2013; Goncalves, Antonio/B-2849-2008; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Melo, Maria Joao/0000-0001-7393-6801; Muralha, Vania/0000-0001-5488-9069; Goncalves, Antonio/0000-0003-2640-3038; 10

Synthesis and Catalytic Properties in Olefin Epoxidation of Octahedral Dichloridodioxidomolybdenum(VI) Complexes Bearing N,N-Dialkylamide Ligands: Crystal Structure of Mo2O4(mu(2)-O)Cl-2(dmf)(4) - Gago, Sandra; Neves, Patricia; Monteiro, Bernardo; Pessego, Marcia; Lopes, Andre D.; Valente, Anabela A.; Paz, Filipe A. Almeida; Pillinger, Martyn; Moreira, Jose; Silva, Carlos M.; Goncalves, Isabel S. (European Journal of Inorganic Chemistry, 2009)
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Times Cited: 22 Neves, Patricia/F-6098-2011; Pessego, Marcia/E-1790-2012; Lopes, Andre/E-2136-2012; Gago, Sandra/H-5413-2012; Moreira, Jose/F-3807-2010; Monteiro, Bernardo/C-6940-2013; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Valente, Anabela/H-5922-2013; REQUIMTE, PHOTO/M-4229-2013; CIQA, CIQA/N-6549-2013; Almeida Paz, Filipe/F-3430-2010; REQUIMTE, LAQV/N-9835-2013 Pessego, Marcia/0000-0001-7292-0568; Lopes, Andre/0000-0002-9702-1216; Gago, Sandra/0000-0003-0221-8508; Moreira, Jose/0000-0001-8481-3436; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Almeida Paz, Filipe/0000-0003-2051-5645; 22

Time?frequency analysis methods to quantify the time-varying microstructure of sleep EEG spindles: Possibility for dementia biomarkers? - Ktonas, PY; Golemati, S.; Xanthopoulos, P.; Sakkalis, V.; Ortigueira, M.D.; Tsekou, H.; Zervakis, M.; Paparrigopoulos, T.; Bonakis, A.; Economou, NT (Journal of Neuroscience Methods, 2009)
The time-varying microstructure of sleep EEG spindles may have clinical significance in dementia studies and can be quantified with a number of techniques. In this paper, real and simulated sleep spindles were regarded as AM?FM signals modeled by six parameters that define the instantaneous envelope (IE) and instantaneous frequency (IF) waveforms for a sleep spindle. These parameters were estimated using four different methods, namely the Hilbert transform (HT), complex demodulation (CD), matching pursuit (MP) and wavelet transform (WT). The average error in estimating these parameters was lowest for HT, higher but still less than 10% for CD and MP, and highest (greater than 10%) for WT. The signal distortion induced by the use of a given method was greatest in the case of HT and MP. These two techniques would necessitate the removal of about 0.4 s from the spindle data, which is an important limitation for the case of spindles with duration less than 1 s. Although the CD method may lead to a higher error than HT and MP, it requires a removal of only about 0.23 s of data. An application of this sleep spindle parameterization via the CD method is proposed, in search of efficient EEG-based biomarkers in dementia. Preliminary results indicate that the proposed parameterization may be promising, since it can quantify specific differences in IE and IF characteristics between sleep spindles from dementia subjects and those from aged controls.
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Toxicological evaluation on human colon carcinoma cell line (CaCo-2) of ionic liquids based on imidazolium, guanidinium, ammonium, phosphonium, pyridinium and pyrrolidinium cations - Frade, Raquel F. M.; Rosatella, Andreia A.; Marques, Carolina S.; Branco, Luis C.; Kulkarni, Prashant S.; Mateus, Nuno M. M.; Afonso, Carlos A. M.; Duarte, Catarina M. M. (Green Chemistry, 2009)
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Times Cited: 25 Branco, Luis/C-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Frade, Raquel/J-4555-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; Rosatella, Andreia/M-5674-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Frade, Raquel/0000-0002-1200-2447; Rosatella, Andreia/0000-0002-2691-3110; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 25

Using discrete-variable optimization for CMOS spiral inductor design - P Pereira; Fino, M.H.; Coito, F. V. (Microelectronics (ICM), 2009 International Conference on, 2009)
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{ Paper field effect transistor } - E. Fortunato; Correia, Nuno; Barquinha, Pedro; Costa, Cláudia; Pereira, Lu\'ıs; Gonçalves, Gonçalo; Rodrigo Martins (2009)
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Keywords: cellulose fibers; fet; oxide; oxide field-effect transistor; rf magnetron sputtering; thin films

{Challenges for Europe under value chain restructuring: Contributions to policy debates} - Meil, Pamela; Stratigaki, Maria; Linardos, Petros; Tengblad, Per; Docherty, Peter; Bannink, Duco; Moniz, Antonio; Margarida Paulos; Krings, Bettina; Nierling, Linda (2009)
65844
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Keywords: 2000). Thus systems differ in terms of their exclusivity; Cebriàn & Lallement; closure or segmentation. Corporatist systems in which some workers and sectors are well-regulated tend to protect core workers. Here group identity and divisions between labour would be the standard and discourse of the policy context. Liberal systems are; norms and discourse in which policy is made (O´Reilly; so that the discourse surrounds individual ability and attractiveness on the labour market.; The policy challenges presented here have been derived from the empirical results obtained from the WORKS quantitative and qualitative data. Thus they have been informed by the analysis of survey results and particularly the workplace level analyses on re; we first set out to situate our particular theme in a larger conceptual landscape and address the following issues: - What current policy debates surround the issue under investigation? - What institutions or structures currently exist that are mainly res; which actors should address the challenges? Policy formulation is refracted through the different social structural frameworks in different countries. Institutional and historical contexts shape these structures. The contexts also shape expectations; which laid the foundation for identifying policy challenges. In the complex and diverse area of policy presented here

{Selective floating gate non-volatile paper memory transistor} - Rodrigo Martins; P. Barquinha; L. Pereira; N. Correia; Gonçalves, G.; Ferreira, I.; E. Fortunato (physica status solidi (RRL) - Rapid Research Letters, 2009)
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A new look at the fractional Brownian motion definition - Ortigueira, M.D.; Batista, A.G. (Sixth International Conference on Multibody Systems, Nonlinear Dynamics and Control (MSNDC), ASME IDETC07, 2008)
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A new look at the fractional Brownian motion definition - Ortigueira, M.D.; Batista, A.G. (Sixth International Conference on Multibody Systems, Nonlinear Dynamics and Control (MSNDC), ASME IDETC07, 2008)
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Modelling metallothionein induction in the liver of Sparus aurata exposed to metal-contaminated sediments - Costa, P. M.; Repolho, T.; Caeiro, S.; Diniz, M. E.; Moura, I.; Costa, M. H. (Ecotoxicology and Environmental Safety, 2008)
Metallothionein (MT) in the liver of gilthead seabreams (Sparus aurata L., 1758) exposed to Sado estuary (Portugal) sediments was quantified to assess the MT induction potential as a biomarker of sediment-based contamination by copper (Cu), cadmium (U), lead (Pb) and arsenic (As). Sediments were collected from two control sites and four sites with different levels of contamination. Sediment Cu, Cd, Pb, As, total organic matter (TOM) and fine fraction (FF) levels were determined. Generalized linear models (GLM) allowed integration of sediment parameters with liver Cu, Cd, Pb, As and MT concentrations. Although sediment metal levels were lower than expected, we relate NIT with liver Cd and also with interactions between liver and sediment Cu and between liver Cu and TOM. We suggest integrating biomarkers and environmental parameters using statistical models such as GLM as a more sensitive and reliable technique for sediment risk assessment than traditional isolated biomarker approaches. (C) 2007 Elsevier Inc. All rights reserved.
Times Cited: 10

Characterization of adrenaline and adrenaline-GSH adduct transport in freshly isolated rat cardiomyocytes - Costa, Vera M.; Ferreira, Luisa M.; Branco, Paula S.; Carvalho, Felix; Bastos, Maria L.; Carvalho, Rui A.; Carvalho, Marcia; Remiao, Fernando (TOXICOLOGY LETTERS, 2008)
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45th Congress of the European-Societies-of-Toxicology, Rhodes, GREECE, OCT 05-08, 2008

Electron transfer complex between nitrous oxide reductase and cytochrome c552 from Pseudomonas nautica: kinetic, nuclear magnetic resonance, and docking studies - Dell'Acqua, S.; Pauleta, S. R.; Monzani, E.; Pereira, A. S.; Casella, L.; Moura, J. J.; Moura, I. (Biochemistry, 2008)
The multicopper enzyme nitrous oxide reductase (N 2OR) catalyzes the final step of denitrification, the two-electron reduction of N 2O to N 2. This enzyme is a functional homodimer containing two different multicopper sites: CuA and CuZ. CuA is a binuclear copper site that transfers electrons to the tetranuclear copper sulfide CuZ, the catalytic site. In this study, Pseudomonas nautica cytochrome c 552 was identified as the physiological electron donor. The kinetic data show differences when physiological and artificial electron donors are compared [cytochrome vs methylviologen (MV)]. In the presence of cytochrome c 552, the reaction rate is dependent on the ET reaction and independent of the N 2O concentration. With MV, electron donation is faster than substrate reduction. From the study of cytochrome c 552 concentration dependence, we estimate the following kinetic parameters: K m c 552 = 50.2 +/- 9.0 muM and V max c 552 = 1.8 +/- 0.6 units/mg. The N 2O concentration dependence indicates a K mN 2 O of 14.0 +/- 2.9 muM using MV as the electron donor. The pH effect on the kinetic parameters is different when MV or cytochrome c 552 is used as the electron donor (p K a = 6.6 or 8.3, respectively). The kinetic study also revealed the hydrophobic nature of the interaction, and direct electron transfer studies showed that CuA is the center that receives electrons from the physiological electron donor. The formation of the electron transfer complex was observed by (1)H NMR protein-protein titrations and was modeled with a molecular docking program (BiGGER). The proposed docked complexes corroborated the ET studies giving a large number of solutions in which cytochrome c 552 is placed near a hydrophobic patch located around the CuA center.
1520-4995 (Electronic)0006-2960 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Cytochrome c Group/*chemistry; Electron Transport; Kinetics; Nuclear Magnetic Resonance, Biomolecular; Oxidoreductases/*chemistry; Pseudomonas/*enzymology

The 1st ISA forum of sociology on the “Sociological Research and Public Debate” - Margarida R. Paulos; Moniz, António B. (Enterprise and Work Innovation Studies, 2008)
1st ISA Forum report
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Keywords: International; sociology

Assessing scenarios on the future of work - António Brandão Moniz (Enterprise and Work Innovation Studies, 2008)
In this paper will be discussed different types of scenarios and the aims for using scenarios. Normaly they are being used by organisations due to the need to anticipate processes, to support policy-making and to understand the complexities of relations. Such organisations can be private companies, R&D organisations and networks of organisations, or even by some public administration institutions. Some cases will be discussed as the methods for ongoing scenario-building process (Shell Internacional). Scenarios should anticipate possible relations among social actors as in the Triple Helix Model, and is possible to develop strategic intelligence in the innovation process that would enable the construction of scenarios. Such processes can be assessed. The focus will be made in relation to the steps chosen for the WORKS scenarios. In this case is there a model of work changes that can be used for foresight? Differences according to sectors were found, as well on other dimensions. Problems of assessment are analysed with specific application to the scenario construction methods.
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Keywords: assessment; foresight; scenarios

Detecção e análise do Potencial Evocado P300 - Diogo Barreiros (FCT-UNL, 2008)
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Editorial Note - António Moniz; José Miquel Cabeças (Enterprise and Work Innovation Studies, 2008)
No abstract is available for this item.
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Fragmentation? The future of work in Europe in a global economy: the WORKS final International Conference debate - Margarida R. Paulos; Moniz, António B. (Enterprise and Work Innovation Studies, 2008)
WORKS final conference report
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Keywords: Future; Work

{Assessing scenarios on the future of work} - António Brandão Moniz (Enterprise and Work Innovation Studies, 2008)
In this paper will be discussed different types of scenarios and the aims for using scenarios. Normaly they are being used by organisations due to the need to anticipate processes, to support policy-making and to understand the complexities of relations. Such organisations can be private companies, R&D organisations and networks of organisations, or even by some public administration institutions. Some cases will be discussed as the methods for ongoing scenario-building process (Shell Internacional). Scenarios should anticipate possible relations among social actors as in the Triple Helix Model, and is possible to develop strategic intelligence in the innovation process that would enable the construction of scenarios. Such processes can be assessed. The focus will be made in relation to the steps chosen for the WORKS scenarios. In this case is there a model of work changes that can be used for foresight? Differences according to sectors were found, as well on other dimensions. Problems of assessment are analysed with specific application to the scenario construction methods.
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Keywords: assessment; foresight; scenarios

Editorial Note - António Moniz; José Miquel Cabeças (Enterprise and Work Innovation Studies, 2008)
No abstract is available for this item.
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{Fragmentation? The future of work in Europe in a global economy: the WORKS final International Conference debate} - Margarida R. Paulos; Moniz, António B. (Enterprise and Work Innovation Studies, 2008)
WORKS final conference report
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Keywords: Future; Work

{Synopsis of global scenario and forecasting surveys scenarios in risk habitat megacity (RHM)} - Woll, Tobias (Enterprise and Work Innovation Studies, 2008)
The main objective of the paper is to provide a synopsis of global scenario and forecasting surveys. First, the paper will give an overview on existing global scenario and forecasting surveys and their specific scenario philosophies and storylines. Second, the major driving forces that shape and characterise the different scenarios will be identified. The scenario analysis has been provided for the research project Risk Habitat Megacity (HRM) that aims at developing strategies for sustainable development in megacities and urban agglomerations. The analysis of international scenario surveys is an essential component within RHM. The scenario analysis will be the basis and source for the development of own RHM-framework scenarios and for defining specific driving forces of change.
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Keywords: megacities; risk habitat; scenarios

{The 1st ISA forum of sociology on the “Sociological Research and Public Debate”} - Margarida R. Paulos; Moniz, António B. (Enterprise and Work Innovation Studies, 2008)
1st ISA Forum report
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Keywords: International; sociology

Biochemical and spectroscopic characterization of the membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617 - Correia, C.; Besson, S.; Brondino, C. D.; Gonzalez, P. J.; Fauque, G.; Lampreia, J.; Moura, I.; Moura, J. J. (J Biol Inorg Chem, 2008)
Membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617 can be solubilized in either of two ways that will ultimately determine the presence or absence of the small (Iota) subunit. The enzyme complex (NarGHI) is composed of three subunits with molecular masses of 130, 65, and 20 kDa. This enzyme contains approximately 14 Fe, 0.8 Mo, and 1.3 molybdopterin guanine dinucleotides per enzyme molecule. Curiously, one heme b and 0.4 heme c per enzyme molecule have been detected. These hemes were potentiometrically characterized by optical spectroscopy at pH 7.6 and two noninteracting species were identified with respective midpoint potentials at Em=+197 mV (heme c) and -4.5 mV (heme b). Variable-temperature (4-120 K) X-band electron paramagnetic resonance (EPR) studies performed on both as-isolated and dithionite-reduced nitrate reductase showed, respectively, an EPR signal characteristic of a [3Fe-4S]+ cluster and overlapping signals associated with at least three types of [4Fe-4S]+ centers. EPR of the as-isolated enzyme shows two distinct pH-dependent Mo(V) signals with hyperfine coupling to a solvent-exchangeable proton. These signals, called "low-pH" and "high-pH," changed to a pH-independent Mo(V) signal upon nitrate or nitrite addition. Nitrate addition to dithionite-reduced samples at pH 6 and 7.6 yields some of the EPR signals described above and a new rhombic signal that has no hyperfine structure. The relationship between the distinct EPR-active Mo(V) species and their plausible structures is discussed on the basis of the structural information available to date for closely related membrane-bound nitrate reductases.
0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Alteromonadaceae/*enzymology; Cell Membrane/*metabolism; Electron Spin Resonance Spectroscopy; Electrophoresis; Guanine Nucleotides/analysis/chemistry/metabolism; Heme/chemistry/metabolism; Hydrogen-Ion Concentration; Iron/chemistry/metabolism; Molecular Weight; Molybdenum/chemistry/metabolism; Nitrate Reductase/analysis/*chemistry/*metabolism; Oxidation-Reduction; Potentiometry; Pterins/analysis/chemistry/metabolism; Spectrophotometry, Ultraviolet; Temperature

Colorimetric method and kit for the detection of specific nucleic acid sequences using metal nanoparticles functionalized with modified oligonucleotides - Tavares, Jose Ricardo Ramos Franco; Baptista, Pedro Miguel Ribeiro Viana; Doria, Goncalo Maria Reimao Pinto De Franca; Flores, Alcino Orfeu De Leao (2008)
The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.
WO2008135929; C12Q 1/ 68 A I

Direct electrochemical study of the multiple redox centers of hydrogenase from Desulfovibrio gigas - Cordas, C. M.; Moura, I.; Moura, J. J. (Bioelectrochemistry, 2008)
Direct electrochemical response was first time observed for the redox centers of Desulfovibrio gigas [NiFe]-Hase, in non-turnover conditions, by cyclic voltammetry, in solution at glassy carbon electrode. The activation of the enzyme was achieved by reduction with H(2) and by electrochemical control and electrocatalytic activity was observed. The inactivation of the [NiFe]-Hase was also attained through potential control. All electrochemical data was obtained in the absence of enzyme inhibitors. The results are discussed in the context of the proposed mechanism currently accepted for activation/inactivation of [NiFe]-Hases.
1878-562X (Electronic)1567-5394 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: *Electrochemistry; Bacterial Proteins; Desulfovibrio gigas/*enzymology; Electrodes; Enzyme Activation; Hydrogen/chemistry; Hydrogenase/*chemistry/metabolism; Iron; Nickel; Oxidation-Reduction

Enzymatic activity mastered by altering metal coordination spheres - Moura, I.; Pauleta, S. R.; Moura, J. J. (J Biol Inorg Chem, 2008)
Metalloenzymes control enzymatic activity by changing the characteristics of the metal centers where catalysis takes place. The conversion between inactive and active states can be tuned by altering the coordination number of the metal site, and in some cases by an associated conformational change. These processes will be illustrated using heme proteins (cytochrome c nitrite reductase, cytochrome c peroxidase and cytochrome cd1 nitrite reductase), non-heme proteins (superoxide reductase and [NiFe]-hydrogenase), and copper proteins (nitrite and nitrous oxide reductases) as examples. These examples catalyze electron transfer reactions that include atom transfer, abstraction and insertion.
0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov'tReview
Keywords: Catalytic Domain; Enzymes/*chemistry/*metabolism; Heme/chemistry/metabolism; Hemeproteins/chemistry/metabolism; Metalloproteins/*chemistry/*metabolism; Metals/*chemistry/*metabolism

Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucleicos através de nanopartículas metálicas funcionalizadas com oligonucleótidos modificados - Tavares, José Ricardo Ramos Franco; Baptista, Pedro Miguel Ribeiro Viana; Dória, Goncalo Maria Reimao Pinto De Franca; Flores, Alcino Orfeu de Leão (2008)
O presente invento relaciona-se com um método colorimétrico de detecção de sequências específicas de ácidos nucleicos, incluindo mutações ou polimorfismos de nucleótido único em sequências de ácidos nucleicos, através da agregação de nanopartículas funcionalizadas com oligonucleótidos modificados induzida por um aumento da força iónica do meio. Outro aspecto do presente invento relaciona-se com o desenvolvimento de um estojo que ao aplicar a metodologia objecto da presente invenção, permite a rápida e fácil detecção de sequências específicas de ácidos nucleicos, incluindo mutações ou polimorfismos de nucleótido único em sequências de ácidos nucleicos.
PT103730; G01N 33/ 58 A I, C12M1/00, C12Q1/68,G01N33/52,G01N33/53

A new type of metal-binding site in cobalt- and zinc-containing adenylate kinases isolated from sulfate-reducers Desulfovibrio gigas and Desulfovibrio desulfuricans ATCC 27774 - Gavel, O. Y.; Bursakov, S. A.; Di Rocco, G.; Trincao, J.; Pickering, I. J.; George, G. N.; Calvete, J. J.; Shnyrov, V. L.; Brondino, C. D.; Pereira, A. S.; Lampreia, J.; Tavares, P; et al. (J Inorg Biochem, 2008)
Adenylate kinase (AK) mediates the reversible transfer of phosphate groups between the adenylate nucleotides and contributes to the maintenance of their constant cellular level, necessary for energy metabolism and nucleic acid synthesis. The AK were purified from crude extracts of two sulfate-reducing bacteria (SRB), Desulfovibrio (D.) gigas NCIB 9332 and Desulfovibrio desulfuricans ATCC 27774, and biochemically and spectroscopically characterised in the native and fully cobalt- or zinc-substituted forms. These are the first reported adenylate kinases that bind either zinc or cobalt and are related to the subgroup of metal-containing AK found, in most cases, in Gram-positive bacteria. The electronic absorption spectrum is consistent with tetrahedral coordinated cobalt, predominantly via sulfur ligands, and is supported by EPR. The involvement of three cysteines in cobalt or zinc coordination was confirmed by chemical methods. Extended X-ray absorption fine structure (EXAFS) indicate that cobalt or zinc are bound by three cysteine residues and one histidine in the metal-binding site of the "LID" domain. The sequence 129Cys-X5-His-X15-Cys-X2-Cys of the AK from D. gigas is involved in metal coordination and represents a new type of binding motif that differs from other known zinc-binding sites of AK. Cobalt and zinc play a structural role in stabilizing the LID domain.
0162-0134 (Print)0162-0134 (Linking)Journal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.
Keywords: Absorptiometry, Photon; Adenylate Kinase/*chemistry/isolation & purification/metabolism; Amino Acid Sequence; Apoenzymes/metabolism; Binding Sites; Cobalt/*chemistry; Desulfovibrio desulfuricans/*enzymology; Desulfovibrio gigas/*enzymology; Electron Spin Resonance Spectroscopy; Molecular Sequence Data; Molecular Weight; Osmolar Concentration; Protein Structure, Tertiary; Pyrimidines; Sequence Alignment; Spectrophotometry, Ultraviolet; Zinc/*chemistry

Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - an NMR, X-ray crystallography and computational chemistry approach - Viegas, Aldino; Bras, Natercia F.; Cerqueira, Nuno M. F. S. A.; Fernandes, Pedro Alexandrino; Prates, Jose A. M.; Fontes, Carlos M. G. A.; Bruix, Marta; Romao, Maria Joao; Carvalho, Ana Luisa; Ramos, Maria Joao; Macedo, Anjos L.; Cabrita, Eurico J. (Febs Journal, 2008)
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Futures of automobile industry and challenges on sustainable development and mobility - António Moniz; Paulos, Margarida Ramires (University Library of Munich, Germany, 2008)
Portugal had only very few foresight exercises on the automobile sector, and the most recent one was a survey held in a project on work organisation systems in the automobile industry, its recent historical paths and the special strategies of location of companies (the WorTiS project). This involved several teams with different disciplinary backgrounds and from two Portuguese universities. The provisional main results of the first round of a Delphi survey held in Portugal on the automotive sector were already published, but a further analysis was not yet done. This foresight survey was done under the WorTiS project, developed in 2004 by IET – Research Centre on Enterprise and Work Innovation (at FCT-UNL), and financed by the Portuguese Ministry of Science and Technology. Some of this experience on foresight analysis is also been transferred to other projects, namely the WORKS project on work organisation restructuring in the knowledge society that received the support from EC and still is running. The majority of experts considered having an average of less knowledge in almost all the scenario topics presented. This means that information on the automotive industry is not spread enough among academics or experts in related fields (regional scientists, innovation economists, engineers, sociologists). Some have a good knowledge but in very specialised fields. Others have expertise on foresight, or macroeconomics, or management sciences, but feel insecure on issues related with futures of automobile sector. Nevertheless, we considered specially the topics where the experts considered themselves to have some knowledge. There were no “irrelevant” topics considered as such by the expert panel. There are also no topics that are not considered a need for co-operation. The lack of technological infrastructures was not considered as a hindered factor for the accomplishment of any scenario. The experts’ panel considered no other international competence besides US, Jap
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Keywords: automotive industry; Delphi survey; economical co-operation; scenario; technology

Futures of automobile industry and challenges on sustainable development and mobility - António Brandão Moniz; Margarida Paulos (HAL, 2008)
Portugal had only very few foresight exercises on the automobile sector, and the most recent one was a survey held in a project on work organisation systems in the automobile industry, its recent historical paths and the special strategies of location of companies (the WorTiS project). This involved several teams with different disciplinary backgrounds and from two Portuguese universities. The provisional main results of the first round of a Delphi survey held in Portugal on the automotive sector were already published, but a further analysis was not yet done. This foresight survey was done under the WorTiS project, developed in 2004 by IET – Research Centre on Enterprise and Work Innovation (at FCT-UNL), and financed by the Portuguese Ministry of Science and Technology. Some of this experience on foresight analysis is also been transferred to other projects, namely the WORKS project on work organisation restructuring in the knowledge society that received the support from EC and still is running. The majority of experts considered having an average of less knowledge in almost all the scenario topics presented. This means that information on the automotive industry is not spread enough among academics or experts in related fields (regional scientists, innovation economists, engineers, sociologists). Some have a good knowledge but in very specialised fields. Others have expertise on foresight, or macroeconomics, or management sciences, but feel insecure on issues related with futures of automobile sector. Nevertheless, we considered specially the topics where the experts considered themselves to have some knowledge. There were no “irrelevant” topics considered as such by the expert panel. There are also no topics that are not considered a need for co-operation. The lack of technological infrastructures was not considered as a hindered factor for the accomplishment of any scenario. The experts’ panel considered no other international competence besides US, Japan
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Keywords: automotive industry; Delphi survey; economical co-operation; scenario; technology

Identification of unamplified genomic DNA sequences using gold nanoparticle probes and a novel thin film photodetector - Martins, Rodrigo Ferrão de Paiva; Pedro Baptista; Silva, Leonardo Bione; Leandro Raniero; Gonçalo Dória; Ricardo Franco; E. Fortunato (Journal of Non-Crystalline Solids, 2008)
This paper describes a novel colorimetric method for detection of nucleic acid targets in a homogeneous format with improved sensitivity by means of a system based on the combination of a tunable monochromatic light source and an amorphous/nanocrystalline silicon photodetector that detects color and light intensity changes undergone by samples/assays containing tailored gold nanoparticles probes. This new low cost, portable, fast and simple optoelectronic platform, with the possibility to be re-used, permits detection of at least 400 fentomole of specific DNA sequences without target or signal amplification and was applied to the rapid detection of human pathogens in large variety of clinical samples such as Mycobacterium tuberculosis.
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Keywords: Biosensors; nanoparticles; sensors; Silicon

Influence of the protein staining in the fast ultrasonic sample treatment for protein identification through peptide mass fingerprint and matrix-assisted laser desorption ionization time of flight mass spectrometry - Galesio, M.; Vieira, D. V.; Rial-Otero, R.; Lodeiro, C.; Moura, I.; Capelo, J. L. (Journal of Proteome Research, 2008)
The influence of the protein staining used to visualize protein bands, after in-gel protein separation, for the correct identification of proteins by peptide mass fingerprint (PMF) after application of the ultrasonic in-gel protein protocol was studied. Coomassie brilliant blue and silver nitrate, both visible stains, and the fluorescent dyes Sypro Red and Sypro Orange were evaluated. Results obtained after comparison with the overnight in-gel protocol showed that good results, in terms of protein sequence coverage and number of peptides matched, can be obtained with anyone of the four stains studied. Two minutes of enzymatic digestion time was enough for proteins stained with coomassie blue, while 4 min was necessary when silver or Sypro stainings were employed in order to reach equivalent results to those obtained for the overnigh in-gel protein protocol. For the silver nitrate stain, the concentration of silver present in the staining solution must be 0.09% (w/v) to minimize background in the MALDI mass spectra.
Times Cited: 8

{Futures of automobile industry and challenges on sustainable development and mobility} - Moniz, António B.; Paulos, Margarida Ramires (University Library of Munich, Germany, 2008)
Portugal had only very few foresight exercises on the automobile sector, and the most recent one was a survey held in a project on work organisation systems in the automobile industry, its recent historical paths and the special strategies of location of companies (the WorTiS project). This involved several teams with different disciplinary backgrounds and from two Portuguese universities. The provisional main results of the first round of a Delphi survey held in Portugal on the automotive sector were already published, but a further analysis was not yet done. This foresight survey was done under the WorTiS project, developed in 2004 by IET – Research Centre on Enterprise and Work Innovation (at FCT-UNL), and financed by the Portuguese Ministry of Science and Technology. Some of this experience on foresight analysis is also been transferred to other projects, namely the WORKS project on work organisation restructuring in the knowledge society that received the support from EC and still is running. The majority of experts considered having an average of less knowledge in almost all the scenario topics presented. This means that information on the automotive industry is not spread enough among academics or experts in related fields (regional scientists, innovation economists, engineers, sociologists). Some have a good knowledge but in very specialised fields. Others have expertise on foresight, or macroeconomics, or management sciences, but feel insecure on issues related with futures of automobile sector. Nevertheless, we considered specially the topics where the experts considered themselves to have some knowledge. There were no “irrelevant” topics considered as such by the expert panel. There are also no topics that are not considered a need for co-operation. The lack of technological infrastructures was not considered as a hindered factor for the accomplishment of any scenario. The experts’ panel considered no other international competence besides US, Japan or Ge
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Keywords: automotive industry; Delphi survey; economical co-operation; scenario; technology

{Futures of automobile industry and challenges on sustainable development and mobility} - António Brandão Moniz; Margarida Paulos (HAL, 2008)
Portugal had only very few foresight exercises on the automobile sector, and the most recent one was a survey held in a project on work organisation systems in the automobile industry, its recent historical paths and the special strategies of location of companies (the WorTiS project). This involved several teams with different disciplinary backgrounds and from two Portuguese universities. The provisional main results of the first round of a Delphi survey held in Portugal on the automotive sector were already published, but a further analysis was not yet done. This foresight survey was done under the WorTiS project, developed in 2004 by IET – Research Centre on Enterprise and Work Innovation (at FCT-UNL), and financed by the Portuguese Ministry of Science and Technology. Some of this experience on foresight analysis is also been transferred to other projects, namely the WORKS project on work organisation restructuring in the knowledge society that received the support from EC and still is running. The majority of experts considered having an average of less knowledge in almost all the scenario topics presented. This means that information on the automotive industry is not spread enough among academics or experts in related fields (regional scientists, innovation economists, engineers, sociologists). Some have a good knowledge but in very specialised fields. Others have expertise on foresight, or macroeconomics, or management sciences, but feel insecure on issues related with futures of automobile sector. Nevertheless, we considered specially the topics where the experts considered themselves to have some knowledge. There were no “irrelevant” topics considered as such by the expert panel. There are also no topics that are not considered a need for co-operation. The lack of technological infrastructures was not considered as a hindered factor for the accomplishment of any scenario. The experts' panel considered no other international competence besides US, Japan or Ge
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Keywords: automotive industry; Delphi survey; economical co-operation; scenario; technology

Detection and quantification system of biological matter constituted by one or more optical sensors and one or more light sources, associated process and related applications - Martins, Rodrigo Ferrao De Paiva; Baptista, Pedro Miguel Ribeiro Viana; Fortunato, Elvira Maria Correia (2008)
The present invention relates to a system and process for detection and/or qualitative and quantitative identification of the biological material, such as specific sequences of nucleic acids or proteins as antibodies, present in biological samples. The system is composed by one or more light sources (1) combined with one or more integrated optical photo sensors, or not, and various electronic components (4), necessary for obtaining/ processing of the signal emitted by the metal nanoprobes functionalized with a solution of biological composite, as well as also a micro-controller and a microprocessor, fixed or portable. This photosensor structure is able to detect and to quantify the colour variations produced by metal nanoprobes, being this preferentially gold, functionalized by oligonucleotides complementary to specific DNA/RNA sequences, proteins, as for instance antibodies and/or antigens related with certain disease, or other sample or solution of biological composite, that are to be investigated. The detection and quantification process is based on the response of a photosensor, singular or integrated, based on thin film technology of amorphous, nanocrystalline or microcrystalline silicon and their alloys, as well as the new active ceramic semiconductors, amorphous and not amorphous.
WO2008029374; C12Q1/68,G01N21/25,G01N33/543

Sistema de detecção e quantificação de matéria biológica constituído por um ou mais sensores ópticos e uma ou mais fontes luminosas, processo associado e respectivas utilizações - Martins, Rodrigo Ferrao De Paiva; Pedro, Miguel Ribeiro Viana Baptista; Fortunato, Elvira Maria Correia (2008)
O presente invento relaciona-se com um novo sistema e processo para detecção e/ou identificação qualitativa e quantitativa de matéria biológica, tais como sequências específicas de ácidos nucleicos ou proteínas, como anticorpos, presentes em amostras biológicas. O sistema é constituído por uma ou mais fontes luminosas combinadas, com um ou mais fotossensores ópticos integrados, ou não, e componentes electrónicos vários, necessários para obtenção/condicionamento do sinal emitido por nanossondas de metal funcionalizadas com a solução de composto biológico, bem como ainda um micro-controlador e um microprocessador, portável ou fixo. Este fotossensor é capaz de detectar e quantificar as diferenças colorimétricas produzidas por nanossondas de metal, sendo este preferencialmente o ouro, funcionalizadas por oligonucleotídeos complementares às sequências específicas de ADN/ARN, proteínas, como por exemplo anticorpos e/ou antigénios relacionados com determinada doença, ou outra amostra ou solução de composto biológico, que se pretende pesquisar. O processo de detecção e quantificação baseia-se na resposta de um fotossensor, singular ou integrado, baseado na tecnologia de filmes finos de sílicio amorfo, nanocristalino ou mícrocristalino, e suas ligas, e também nos novos cerâmicos semicondutores activos, amorfos e não morfos. O referido sistema e processo de detecção e/ou identificação de matéria biológica tem aplicação na biotecnologia, incluindo a biomedicina.
PT103561; G01N 33/ 52 A I

Análise de Fusos do Sono: decomposição e desmodulação de sinal - Diogo N. Leitão (FCT-UNL, 2008)
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Benefits of membrane electrodes in the electrochemistry of metalloproteins: mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by horse cytochrome c: a case study - Paes de Sousa, P. M.; Pauleta, S. R.; Rodrigues, D.; Simoes Goncalves, M. L.; Pettigrew, G. W.; Moura, I.; Moura, J. J.; Correia dos Santos, M. M. (J Biol Inorg Chem, 2008)
A comparative study of direct and mediated electrochemistry of metalloproteins in bulk and membrane-entrapped solutions is presented. This work reports the first electrochemical study of the electron transfer between a bacterial cytochrome c peroxidase and horse heart cytochrome c. The mediated catalysis of the peroxidase was analysed both using the membrane electrode configuration and with all proteins in solution. An apparent Michaelis constant of 66 +/- 4 and 42 +/- 5 microM was determined at pH 7.0 and 0 M NaCl for membrane and bulk solutions, respectively. The data revealed that maximum activity occurs at 50 mM NaCl, pH 7.0, with intermolecular rate constants of (4.4 +/- 0.5) x 10(6) and (1.0 +/- 0.5) x 10(6) M(-1) s(-1) for membrane-entrapped and bulk solutions, respectively. The influence of parameters such as pH or ionic strength on the mediated catalytic activity was analysed using this approach, drawing attention to the fact that careful analysis of the results is needed to ensure that no artefacts are introduced by the use of the membrane configuration and/or promoters, and therefore the dependence truly reflects the influence of these parameters on the (mediated) catalysis. From the pH dependence, a pK of 7.5 was estimated for the mediated enzymatic catalysis.
0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Animals; Catalysis; Cytochrome-c Peroxidase/*chemistry; Cytochromes c/*chemistry; Electrochemistry; Electrodes; Electron Transport; Horses; Hydrogen Peroxide/chemistry; Hydrogen-Ion Concentration; Membranes, Artificial; Metalloproteins/*chemistry; Myocardium/enzymology; Paracoccus pantotrophus/*enzymology; Potentiometry

Characterization of optoelectronic platform using an amorphous/nanocrystalline silicon biosensor for the specific identification of nucleic acid sequences based on gold nanoparticle probes - Silva, L. B.; Pedro Baptista; Leandro Raniero; Gonçalo Doria; Martins, Rodrigo Ferrão de Paiva; Fortunato, Elvira Maria Correia (Sensors and Actuators B: Chemical, 2008)
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The globalisation in the clothing sector and its implications for work organisation: a view from the Portuguese case - Moniz, António B.; Margarida R. Paulos (Universidade Nova de Lisboa, IET-Research on Enterprise and Work Innovation, Faculty of Science and Technology, 2008)
The clothing sector in Portugal is still seen, in many aspects as a traditional sector with some average characteristics, such as: low level of qualifications, less flexible labour legislation and stronger unionisation, very low salaries and low capability of investment in innovation and new technology. Is, nevertheless, a very important sector in terms of labour market, with increased weight in the exporting structure. Globalisation and delocalisation are having a strong impact in the organisation of work and in occupational careers in the sector. With the pressure of global competitiveness in what concerns time and prices, very few companies are able to keep a position in the market without changes in organisation of work and workers. And those that can perform good responses to such challenges are achieving a better economical stability. The companies have found different ways to face this reality according to size, capital and position. We could find two main paths: one where companies outsource a part or the entire production to another territory (for example, several manufacturing tasks), close and/or dismissal the workers. Other path, where companies up skilled their capacities investing, for example, in design, workers training, conception and introduction of new or original products. This paper will present some results from the European project WORKS – Work organisation and restructuring in the knowledge society (6th Framework Programme), focusing the Portuguese case studies in several clothing companies in what concern implications of global context for the companies in general and for the workers in particular, in a comparative analysis with some other European countries.
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Keywords: clothing industry; knowledge society; restructuring; Work

Gold nanoparticles for the development of clinical diagnosis methods - Pedro Baptista; Eulália Pereira; Peter Eaton; Gonçalo Doria; Adelaide Miranda; Inês Gomes; Pedro Quaresma; Ricardo Franco (Analytical and Bioanalytical Chemistry, 2008)
The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticle-based assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles show easily tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.
Sem pdf
Keywords: Bioanalytical methods; Biological samples; Gold nanoparticles; nanoparticles; nanotechnology; Nucleic acids (DNA/RNA)

Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum - Najmudin, S.; Gonzalez, P. J.; Trincao, J.; Coelho, C.; Mukhopadhyay, A.; Cerqueira, N. M.; Romao, C. C.; Moura, I.; Moura, J. J.; Brondino, C. D.; Romao, M. J. (J Biol Inorg Chem, 2008)
Nitrate reductase from Desulfovibrio desulfuricans ATCC 27774 (DdNapA) is a monomeric protein of 80 kDa harboring a bis(molybdopterin guanine dinucleotide) active site and a [4Fe-4S] cluster. Previous electron paramagnetic resonance (EPR) studies in both catalytic and inhibiting conditions showed that the molybdenum center has high coordination flexibility when reacted with reducing agents, substrates or inhibitors. As-prepared DdNapA samples, as well as those reacted with substrates and inhibitors, were crystallized and the corresponding structures were solved at resolutions ranging from 1.99 to 2.45 A. The good quality of the diffraction data allowed us to perform a detailed structural study of the active site and, on that basis, the sixth molybdenum ligand, originally proposed to be an OH/OH(2) ligand, was assigned as a sulfur atom after refinement and analysis of the B factors of all the structures. This unexpected result was confirmed by a single-wavelength anomalous diffraction experiment below the iron edge (lambda = 1.77 A) of the as-purified enzyme. Furthermore, for six of the seven datasets, the S-S distance between the sulfur ligand and the Sgamma atom of the molybdenum ligand Cys(A140) was substantially shorter than the van der Waals contact distance and varies between 2.2 and 2.85 A, indicating a partial disulfide bond. Preliminary EPR studies under catalytic conditions showed an EPR signal designated as a turnover signal (g values 1.999, 1.990, 1.982) showing hyperfine structure originating from a nucleus of unknown nature. Spectropotentiometric studies show that reduced methyl viologen, the electron donor used in the catalytic reaction, does not interact directly with the redox cofactors. The turnover signal can be obtained only in the presence of the reaction substrates. With use of the optimized conditions determined by spectropotentiometric titration, the turnover signal was developed with (15)N-labeled nitrate and in D(2)O-exchanged DdNapA samples. These studies indicate that this signal is not associated with a Mo(V)-nitrate adduct and that the hyperfine structure originates from two equivalent solvent-exchangeable protons. The new coordination sphere of molybdenum proposed on the basis of our studies led us to revise the currently accepted reaction mechanism for periplasmic nitrate reductases. Proposals for a new mechanism are discussed taking into account a molybdenum and ligand-based redox chemistry, rather than the currently accepted redox chemistry based solely on the molybdenum atom.
0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Binding Sites; Catalysis; Crystallography, X-Ray; Data Interpretation, Statistical; Desulfovibrio desulfuricans/*enzymology; Disulfides/chemistry; Electron Spin Resonance Spectroscopy; Ligands; Models, Molecular; Molybdenum/*chemistry; Nitrate Reductase/*metabolism; Protein Conformation; Sulfur/*chemistry

{The globalisation in the clothing sector and its implications for work organisation: a view from the Portuguese case} - Moniz, António B.; Margarida R. Paulos (Universidade Nova de Lisboa, IET/CICS.NOVA-Interdisciplinary Centre on Social Sciences, Faculty of Science and Technology, 2008)
The clothing sector in Portugal is still seen, in many aspects as a traditional sector with some average characteristics, such as: low level of qualifications, less flexible labour legislation and stronger unionisation, very low salaries and low capability of investment in innovation and new technology. Is, nevertheless, a very important sector in terms of labour market, with increased weight in the exporting structure. Globalisation and delocalisation are having a strong impact in the organisation of work and in occupational careers in the sector. With the pressure of global competitiveness in what concerns time and prices, very few companies are able to keep a position in the market without changes in organisation of work and workers. And those that can perform good responses to such challenges are achieving a better economical stability. The companies have found different ways to face this reality according to size, capital and position. We could find two main paths: one where companies outsource a part or the entire production to another territory (for example, several manufacturing tasks), close and/or dismissal the workers. Other path, where companies up skilled their capacities investing, for example, in design, workers training, conception and introduction of new or original products. This paper will present some results from the European project WORKS – Work organisation and restructuring in the knowledge society (6th Framework Programme), focusing the Portuguese case studies in several clothing companies in what concern implications of global context for the companies in general and for the workers in particular, in a comparative analysis with some other European countries.
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Keywords: clothing industry; knowledge society; restructuring; Work

Antineoplastic 31-norcycloartanones from Solanum cernuum Vell. - Grando, Rogerio; Antonio, Marcia A.; Araujo, Carlos E. P.; Soares, Catarina; Medeiros, Maria A.; de Carvalho, Joao E.; Lourenco, Ana M.; Lopes, Luciane C. (ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2008)
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Sarcoplasmic reticulum calcium ATPase is inhibited by organic vanadium coordination compounds: pyridine-2,6-dicarboxylatodioxovanadium(V), BMOV, and an amavadine analogue - Aureliano, M.; Henao, F.; Tiago, T.; Duarte, R. O.; Moura, J. J.; Baruah, B.; Crans, D. C. (Inorg Chem, 2008)
The general affinity of the sarcoplasmic reticulum (SR) Ca (2+)-ATPase was examined for three different classes of vanadium coordination complexes including a vanadium(V) compound, pyridine-2,6-dicarboxylatodioxovanadium(V) (PDC-V(V)), and two vanadium(IV) compounds, bis(maltolato)oxovanadium(IV) (BMOV), and an analogue of amavadine, bis( N-hydroxylamidoiminodiacetato)vanadium(IV) (HAIDA-V(IV)). The ability of vanadate to act either as a phosphate analogue or as a transition-state analogue with enzymes' catalysis phosphoryl group transfer suggests that vanadium coordination compounds may reveal mechanistic preferences in these classes of enzymes. Two of these compounds investigated, PDC-V(V) and BMOV, were hydrolytically and oxidatively reactive at neutral pH, and one, HAIDA-V(IV), does not hydrolyze, oxidize, or otherwise decompose to a measurable extent during the enzyme assay. The SR Ca (2+)-ATPase was inhibited by all three of these complexes. The relative order of inhibition was PDC-V(V) > BMOV > vanadate > HAIDA-V(IV), and the IC 50 values were 25, 40, 80, and 325 microM, respectively. Because the observed inhibition is more potent for PDC-V(V) and BMOV than that of oxovanadates, the inhibition cannot be explained by oxovanadate formation during enzyme assays. Furthermore, the hydrolytically and redox stable amavadine analogue HAIDA-V(IV) inhibited the Ca (2+)-ATPase less than oxovanadates. To gauge the importance of the lipid environment, studies of oxidized BMOV in microemulsions were performed and showed that this system remained in the aqueous pool even though PDC-V(V) is able to penetrate lipid interfaces. These findings suggest that the hydrolytic properties of these complexes may be important in the inhibition of the calcium pump. Our results show that two simple coordination complexes with known insulin enhancing effects can invoke a response in calcium homeostasis and the regulation of muscle contraction through the SR Ca (2+)-ATPase.
1520-510X (Electronic)0020-1669 (Linking)Journal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't
Keywords: Animals; Enzyme Inhibitors/chemistry/pharmacology; inhibitors; Inhibitory Concentration 50; Magnetic Resonance Spectroscopy; Molecular Structure; Organometallic Compounds/*chemistry/pharmacology; Rabbits; Sarcoplasmic Reticulum Calcium-Transporting ATPases/*antagonists &; Structure-Activity Relationship; Vanadates/chemistry/pharmacology; Vanadium/*chemistry

Mechanistic study of intramolecular aldol reactions of dialdehydes - Duarte, Filipe J. S.; Cabrita, Eurico J.; Frenking, Gernot; Santos, Gil (European Journal of Organic Chemistry, 2008)
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Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 - Gavel, O. Y.; Kladova, A. V.; Bursakov, S. A.; Dias, J. M.; Texeira, S.; Shnyrov, V. L.; Moura, J. J.; Moura, I.; Romao, M. J.; Trincao, J. (Acta Crystallogr Sect F Struct Biol Cryst Commun, 2008)
Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP:sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 A resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes.
1744-3091 (Electronic)1744-3091 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: *X-Ray Diffraction; Bacterial Proteins/*chemistry/isolation & purification; Cobalt/chemistry; Crystallization; Desulfovibrio desulfuricans/*enzymology; Enzyme Activation/physiology; Enzyme Stability; Sulfate Adenylyltransferase/*chemistry/isolation & purification; Sulfates/*chemistry; Zinc/chemistry

The globalisation in the clothing sector and its implications for work organisation: a view from the Portuguese case - António Moniz; Paulos, Margarida Ramires (University Library of Munich, Germany, 2008)
The clothing sector in Portugal is still seen, in many aspects as a traditional sector with some average characteristics, such as: low level of qualifications, less flexible labour legislation and stronger unionisation, very low salaries and low capability of investment in innovation and new technology. Is, nevertheless, a very important sector in terms of labour market, with increased weight in the exporting structure. Globalisation and delocalisation are having a strong impact in the organisation of work and in occupational careers in the sector. With the pressure of global competitiveness in what concerns time and prices, very few companies are able to keep a position in the market without changes in organisation of work and workers. And those that can perform good responses to such challenges are achieving a better economical stability. The companies have found different ways to face this reality according to size, capital and position. We could find two main paths: one where companies outsource a part or the entire production to another territory (for example, several manufacturing tasks), close and/or dismissal the workers. Other path, where companies up skilled their capacities investing, for example, in design, workers training, conception and introduction of new or original products. This paper will present some results from the European project WORKS – Work organisation and restructuring in the knowledge society (6th Framework Programme), focusing the Portuguese case studies in several clothing companies in what concern implications of global context for the companies in general and for the workers in particular, in a comparative analysis with some other European countries.
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Keywords: clothing industry; knowledge society; restructuring; Work

{The globalisation in the clothing sector and its implications for work organisation: a view from the Portuguese case} - António Moniz; Paulos, Margarida Ramires (University Library of Munich, Germany, 2008)
The clothing sector in Portugal is still seen, in many aspects as a traditional sector with some average characteristics, such as: low level of qualifications, less flexible labour legislation and stronger unionisation, very low salaries and low capability of investment in innovation and new technology. Is, nevertheless, a very important sector in terms of labour market, with increased weight in the exporting structure. Globalisation and delocalisation are having a strong impact in the organisation of work and in occupational careers in the sector. With the pressure of global competitiveness in what concerns time and prices, very few companies are able to keep a position in the market without changes in organisation of work and workers. And those that can perform good responses to such challenges are achieving a better economical stability. The companies have found different ways to face this reality according to size, capital and position. We could find two main paths: one where companies outsource a part or the entire production to another territory (for example, several manufacturing tasks), close and/or dismissal the workers. Other path, where companies up skilled their capacities investing, for example, in design, workers training, conception and introduction of new or original products. This paper will present some results from the European project WORKS – Work organisation and restructuring in the knowledge society (6th Framework Programme), focusing the Portuguese case studies in several clothing companies in what concern implications of global context for the companies in general and for the workers in particular, in a comparative analysis with some other European countries.
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Keywords: clothing industry; knowledge society; restructuring; Work

Reduction of ascorbate free radical by the plasma membrane of synaptic terminals from rat brain - Samhan-Arias, A. K.; Duarte, R. O.; Martin-Romero, F. J.; Moura, J. J.; Gutierrez-Merino, C. (Arch Biochem Biophys, 2008)
Synaptic plasma membranes (SPMV) decrease the steady state ascorbate free radical (AFR) concentration of 1mM ascorbate in phosphate/EDTA buffer (pH 7), due to AFR recycling by redox coupling between ascorbate and the ubiquinone content of these membranes. In the presence of NADH, but not NADPH, SPMV catalyse a rapid recycling of AFR which further lower the AFR concentration below 0.05 microM. These results correlate with the nearly 10-fold higher NADH oxidase over NADPH oxidase activity of SPMV. SPMV has NADH-dependent coenzyme Q reductase activity. In the presence of ascorbate the stimulation of the NADH oxidase activity of SPMV by coenzyme Q(1) and cytochrome c can be accounted for by the increase of the AFR concentration generated by the redox pairs ascorbate/coenzyme Q(1) and ascorbate/cytochrome c. The NADH:AFR reductase activity makes a major contribution to the NADH oxidase activity of SPMV and decreases the steady-state AFR concentration well below the micromolar concentration range.
1096-0384 (Electronic)0003-9861 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: *Free Radicals; Animals; Ascorbic Acid/*metabolism; Biochemistry/*methods; Brain/*metabolism; Cell Membrane/*metabolism; Cytochromes c/metabolism; Edetic Acid/chemistry; NADPH Oxidase/metabolism; Oxidation-Reduction; Rats; Synapses/*metabolism; Ubiquinone/chemistry

Sub-cellular partitioning of Zn, Cu, Cd and Pb in the digestive gland of native Octopus vulgaris exposed to different metal concentrations (Portugal) - Raimundo, J.; Vale, C.; Duarte, R.; Moura, I. (Science of the Total Environment, 2008)
Cd and Pb and their sub-cellular distributions were determined in Cu Concentrations of Zn,, composite samples of digestive glands of the common octopus, Octopus vulgaris caught from two areas of the Portuguese coast characterised by contrasting metal contamination. Minor contents of Zn (1%), Cu (2%), Cd (6%) and Pb (7%) were found in the insoluble fraction, consisting of nuclei, mitochondria, lysosomes and microsome operationally separated from the whole digestive gland through a sequential centrifugation. A tendency for linear relationships between metal concentrations in nuclei, mitochondria, lysosomes and whole digestive gland was observed. These relationships suggest that despite low metal content organelles responded to the increasing accumulated metals, which means that detoxifying mechanism in cytosol was incomplete. Poorer correlations between microsome and whole digestive gland did not point to metal toxicity in the analysed compartments. However, the high accumulated Cd indicated that O. vulgaris is an important vehicle of this element to its predators in the coastal environment. (c) 2007 Elsevier B.V. All rights reserved.
Times Cited: 6

An improved clean sonoreactor-based method for protein identification by mass spectrometry-based techniques - Santos, H. M.; Mota, Cristiano; Lodeiro, C.; Moura, Isabel; Isaac, Issa; Capelo, J. L. (Talanta, 2008)
A new clean fast (8 min) method for in-solution protein digestion Without detergent or urea for protein identification by peptide mass fingerprint and mass spectrometry-based techniques is Proposed. The new method avoids the use of time consuming desalting procedures entailing the following four steps done under the effect of an ultrasonic field provided by a sonoreactor: denaturation (1 min) in a mixed Solution of water:acetonitrile 1/1 (v/v): protein reduction (1 min); protein alkylation (1 min); and protein digestion (5 min). Five Proteins with masses comprised between 14.4 kDa and 97 kDa and the protein splitsoret cytochrome c from D. desulfuricans ATCC27774, Were Successfully identified with this procedure. No differences were found in the sequence coverage or in the number of peptides matched when the new clean method was compared to another one using urea. Twofold better signal-to-noise ratios were obtained in the MALDI spectra from protein samples prepared with the new method when comparing it with a method using urea. The new digestion method avoids the need to remove salt content and increases throughput (six samples at once) while reducing sample loss and contamination from sample handling. (C) 2008 Elsevier B.V. All rights reserved.
Times Cited: 8

On the HHT, its problems, and some solutions - Rato, R.T.; Manuel Ortigueira; Arnaldo Batista (Mechanical Systems and Signal Processing, 2008)
The empirical mode decomposition (EMD) is reviewed and some questions related to its effective performance are discussed. Its interpretation in terms of AM?FM modulation is done. Solutions for its drawbacks are proposed. Numerical simulations are carried out to empirically evaluate the proposed modified EMD.
Special Issue: Mechatronics

Reaction of aromatic nitroso compounds with chemical models of `thiamine active aldehyde' - Ferreira, Luisa M.; Marques, M. Manuel B.; Gloria, Paulo M. C.; Chaves, Humberto T.; Franco, Joao-Pedro P.; Mourato, Isabel; Antunes, Jose-Rafael T.; Rzepa, Henry S.; Lobo, Ana M; Prabhakar, Sundaresan (TETRAHEDRON, 2008)
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Light activation of transcription: photocaging of nucleotides for control over RNA polymerization - Pinheiro, André Vidal; Pedro Baptistap; Lima, João Carlos (Nucleic Acids Research, 2008)
We describe the use of ATP caged with [7-(diethylamino)coumarin-4-yl]methyl (DEACM) for light-controlled in vitro transcription reactions. Polymerization is blocked when DEACM is bonded to the gamma phosphate group of the ATP molecule. Controlled light irradiation releases ATP and transcription is initiated. In order to provide full control over the process, conditions involved in substrate release, nucleotide availability after release and the effect of the released coumarin in RNA polymerization were assessed in further detail. Together, our data provide the first direct evidence of control over enzymatic polymerization of nucleic acids through light. This approach may provide researchers with a unique tool for the study of biological processes at a molecular level.
The authors would like to acknowledge Dr A. Jorge Parola for fruitful suggestions related to photochemistry procedures, Dr João Noronha for Mass Spectra analysis, Dr Isabel Sá Nogueira and Dr José Inácio for assistance with a-32P-UTP experiments. This work was supported by Fundac¸ ão para a Ciência e Tecnologia (REQUIMTE, CIGMH, PTDC/BIO/66514/2006, PTDC/SAU-BEB/ 66511/2006, FRH/BD/24276/2005 to A.V.P.); Fundac¸ ão Calouste Gulbenkian (Ref. 76436). Funding to pay the Open Access publication charges for this article was provided by Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa.
Keywords: Light; Photolabile protecting; Photolysis

Multiplexed spectral coding for simultaneous detection of DNA hybridization reactions based on FRET - Letícia Giestas; Ferreira, Guilherme N. M.; Baptista, Pedro V; Lima, João Carlos (Sensors and Actuators B: Chemical, 2008)
Fluorescence resonance energy transfer (FRET) is widely used in spectral codification of information at the molecular level, and can be used to generate several layers of information on a DNA chip. We used two oligonucleotides (probes) labeled with different donor (harvesting) molecules in hybridization experiments with complementary oligonucleotides labeled with four different acceptors (targets). By looking at the fluorescence response of the sample after {"}specific{"} excitation of each donor molecule (by {"}specific{"} we mean a wavelength where one of the donors is predominantly excited), we inspected the possibility t o identify the complementary oligonucleotide hybridized to the probe, in mixtures containing two donor probe/acceptor target pairs. In most samples (13 out of the 16 possible), it is trivial to identify the complementary target that is hybridized to the excited donor probe in the mixtures. The major limitations of the chosen system arise when very different concentrations of donor probe/acceptor target pairs are present in the same sample. (C) 2008 Elsevier B.V. All rights reserved.
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The transformation of work? A quantitative evaluation of changes in work in Portugal - Moniz, António B. (Universidade Nova de Lisboa, IET-Research on Enterprise and Work Innovation, Faculty of Science and Technology, 2008)
This report is made for the Work Package 15 of WORKS project and tries to develop more information on the Portuguese situation in the work structures changes in the recent years. It starts with an analysis of socio- economical indicators (Macro economical indicators, Employment indicators, Consumption, Technology at the workplace, Productivity), and then approaches the situation in terms of work flexibility in its dimensions of time use and New forms of work organisation. It traces employment in business functions with a sectoral and occupational approach, and analyses the occupational change in South Europe with particular relevance to Portugal (skill utilisation and job satisfaction, occupational and industrial mobility, quantitative evaluation of the shape of employment in Europe. Finaly are analysed the globalisation indicators.
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Keywords: employment; institutions; investment; labour markets; technology modernisation; work organisation

{The transformation of work? A quantitative evaluation of changes in work in Portugal} - Moniz, António B. (Universidade Nova de Lisboa, IET/CICS.NOVA-Interdisciplinary Centre on Social Sciences, Faculty of Science and Technology, 2008)
This report is made for the Work Package 15 of WORKS project and tries to develop more information on the Portuguese situation in the work structures changes in the recent years. It starts with an analysis of socio- economical indicators (Macro economical indicators, Employment indicators, Consumption, Technology at the workplace, Productivity), and then approaches the situation in terms of work flexibility in its dimensions of time use and New forms of work organisation. It traces employment in business functions with a sectoral and occupational approach, and analyses the occupational change in South Europe with particular relevance to Portugal (skill utilisation and job satisfaction, occupational and industrial mobility, quantitative evaluation of the shape of employment in Europe. Finaly are analysed the globalisation indicators.
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Keywords: employment; institutions; investment; labour markets; technology modernisation; work organisation

Identifying a Transfer Function From a Frequency Response - Duarte Valério; Manuel Duarte Ortigueira; José Sá da Costa (Journal of Computational and Nonlinear Dynamics, 2008)
In this paper, the classic Levy identification method is reviewed and reformulated using a complex representation. This new formulation addresses the well known bias of the classic method at low frequencies. The formulation is generic, coping with both integer order and fractional order transfer functions. A new algorithm based on a stacked matrix and its pseudoinverse is proposed to accommodate the data over a wide range of frequencies. Several simulation results are presented, together with a real system identification. This system is the Archimedes Wave Swing, a prototype of a device to convert the energy of sea waves into electricity.
Special Issue Discontinuous and Fractional Dynamical Systems

Futures of automobile industry and challenges on sustainable development and mobility - Moniz, António B.; Margarida R. Paulos (Universidade Nova de Lisboa, IET-Research on Enterprise and Work Innovation, Faculty of Science and Technology, 2008)
Portugal had only very few foresight exercises on the automobile sector, and the most recent one was a survey held in a project on work organisation systems in the automobile industry, its recent historical paths and the special strategies of location of companies (the WorTiS project). This involved several teams with different disciplinary backgrounds and from two Portuguese universities. The provisional main results of the first round of a Delphi survey held in Portugal on the automotive sector were already published, but a further analysis was not yet done. This foresight survey was done under the WorTiS project, developed in 2004 by IET – Research Centre on Enterprise and Work Innovation (at FCT-UNL), and financed by the Portuguese Ministry of Science and Technology. Some of this experience on foresight analysis is also been transferred to other projects, namely the WORKS project on work organisation restructuring in the knowledge society that received the support from EC and still is running. The majority of experts considered having an average of less knowledge in almost all the scenario topics presented. This means that information on the automotive industry is not spread enough among academics or experts in related fields (regional scientists, innovation economists, engineers, sociologists). Some have a good knowledge but in very specialised fields. Others have expertise on foresight, or macroeconomics, or management sciences, but feel insecure on issues related with futures of automobile sector. Nevertheless, we considered specially the topics where the experts considered themselves to have some knowledge. There were no “irrelevant” topics considered as such by the expert panel. There are also no topics that are not considered a need for co-operation. The lack of technological infrastructures was not considered as a hindered factor for the accomplishment of any scenario. The experts’ panel considered no other international competence besides US, Jap
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Keywords: automotive industry; Delphi survey; economical co-operation; scenario; technology

{Futures of automobile industry and challenges on sustainable development and mobility} - Moniz, António B.; Margarida R. Paulos (Universidade Nova de Lisboa, IET/CICS.NOVA-Interdisciplinary Centre on Social Sciences, Faculty of Science and Technology, 2008)
Portugal had only very few foresight exercises on the automobile sector, and the most recent one was a survey held in a project on work organisation systems in the automobile industry, its recent historical paths and the special strategies of location of companies (the WorTiS project). This involved several teams with different disciplinary backgrounds and from two Portuguese universities. The provisional main results of the first round of a Delphi survey held in Portugal on the automotive sector were already published, but a further analysis was not yet done. This foresight survey was done under the WorTiS project, developed in 2004 by IET – Research Centre on Enterprise and Work Innovation (at FCT-UNL), and financed by the Portuguese Ministry of Science and Technology. Some of this experience on foresight analysis is also been transferred to other projects, namely the WORKS project on work organisation restructuring in the knowledge society that received the support from EC and still is running. The majority of experts considered having an average of less knowledge in almost all the scenario topics presented. This means that information on the automotive industry is not spread enough among academics or experts in related fields (regional scientists, innovation economists, engineers, sociologists). Some have a good knowledge but in very specialised fields. Others have expertise on foresight, or macroeconomics, or management sciences, but feel insecure on issues related with futures of automobile sector. Nevertheless, we considered specially the topics where the experts considered themselves to have some knowledge. There were no “irrelevant” topics considered as such by the expert panel. There are also no topics that are not considered a need for co-operation. The lack of technological infrastructures was not considered as a hindered factor for the accomplishment of any scenario. The experts’ panel considered no other international competence besides US, Japan or Ge
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Keywords: automotive industry; Delphi survey; economical co-operation; scenario; technology

Long-term Energy Scenarios Under Uncertainty - Patrícia Fortes; Simões, Sofia; Cleto, João; Júlia Seixas (5th International Conference on the European Electricity Market, 2008)

Renewable Energy Sources Availability under Climate Change Scenarios – impacts on the Portuguese Energy System - Cleto, João; Simões, Sofia; Fortes, Patricia; Júlia Seixas (5th International Conference on the European Electricity Market, 2008)

2 '-hydroxyflavylium: introducing flavanones into the flavylium network of chemical reactions - Petrov, V.; Gomes, R; A. J. Parola; Jesus, A.; Laia, C. A. T.; Pina, F (Tetrahedron, 2008)
Chalcones possessing a hydroxyl group in position 2 cyclize to form flavylium salts in acidic media, this reaction being reversible under neutral-basic conditions. On the other hand, chalcones possessing a hydroxyl group in position 2' cyclize to form flavanones in basic media. By synthesizing 2'-hydroxyflavylium tetrafluoroborate, it was possible to obtain trans-2,2'-dihydroxychalcone that in solution can evolve to 2'-hydroxyflavanone or back to 2'-hydroxyflavylium depending on the pH. The several equilibria established in aqueous solution were fully characterized. The importance of including flavanones into the flavylitun network of chemical reactions is briefly exploited. (C) 2007 Elsevier Ltd. All rights reserved.
ISI Document Delivery No.: 257SHTimes Cited: 5 Cited Reference Count: 31 Petrov, Vesselin Gomes, Raquel Parola, A. Jorge Jesus, Alexandre Laia, Cesar A. T. Pina, Fernando Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Parola, A. Jorge/0000-0002-1333-9076; Pergamon-elsevier science ltd Oxford

Advances in NDT techniques for friction stir welding joints of AA2024 - Santos, T.; Vilaça, P.; Reis, L.; Quintino, L.; De Freitas, M. (TMS 2008 Annual Meeting Supplemental: Materials Processing and Properties, 2008)
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Conference code: 73623Cited By :2Export Date: 27 February 2015CODEN: 85MVA

Application of diffusion-ordered spectroscopy for the analysis of cancer related biological samples - Ivanova, G. I.; E. J. Cabrita; O’Connor, R.; Eustace, A. J.; Brougham, D. F. (Bulgarian Chemical Communications, 2008)
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Data fusion in non destructive testing using fuzzy logic to evaluate friction stir welding - Dos Santos, T.G.; Silva, B.S.; Dos Santos Vilaça, P.; Quintino, L.; Sousa, J.M.C. (Welding International, 2008)
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Cited By :4Export Date: 27 February 2015

Developments in NDT for detecting imperfections in friction stir welds in aluminium alloys - Santos, T.; Vilaça, P.; Quintino, L. (Welding in the World, 2008)
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Cited By :8Export Date: 27 February 2015CODEN: WDWRA

Electrocolorimetry of electrochromic materials on flexible ITO electrodes - Pinheiro, C; A. J. Parola; Pina, F; Fonseca, J.; Freire, C. (Solar Energy Materials and Solar Cells, 2008)
Electrochromic materials are characterized by their colour changes upon applied voltage. Colour can mean many things: a certain kind of light, its effect on the human eye, or the result of this effect in the mind of the viewer. Since the electrochromic materials are developed towards real life applications it is relevant to characterize them with the usual commercial colour standards. A colorimetric study of electrogenerated Prussian blue and electrogenerated polymers based on salen-type complexes of Cu(II), Ni(II) and Pd(H) deposited over transparent flexible electrodes of polyethylene terephthalate coated with indium tin oxide (PET/ITO electrodes) was carried out using the CIELAB coordinates. A cuvette with a designed adapter to allow potentiostatic control was placed on an integrating sphere installed in the sample compartment of a spectrophotometer to run the colorimetric measurements. The colour evolution in situ was measured through the transmittance of the films by potentiostatic control. Chronocoutometry/chronoabsorptometry was used to evaluate maximum coloration efficiencies for the coloration step: 184 (Pd), 161 (Cu) and 83 cm(2)/C (Ni) and for bleaching: 199 (Pd), 212 (Cu) and 173 cm(2)/C (Ni) of the Pd, Cu and Ni polymer films, respectively. The Prussian Blue/Prussian White states over the PET/ITO films were relatively reversible while the reversibility and stability of the polymers based on the metals salen-type complexes depends on the metal, Pd being the most stable. (c) 2008 Elsevier B.V. All rights reserved.
ISI Document Delivery No.: 322DZTimes Cited: 8 Cited Reference Count: 20 Pinheiro, Carlos Parola, A. J. Pina, F. Fonseca, J. Freire, C. 7th International Meeting on Electrochromism Sep 03-07, 2006 Istanbul, TURKEY Pina, Fernando/C-8161-2011; Freire, Cristina/F-4775-2010; Parola, A. Jorge/F-4048-2010 Parola, A. Jorge/0000-0002-1333-9076 Elsevier science bv Amsterdam

Flavylium chromophores as species markers for dragon's blood resins from Dracaena and Daemonorops trees - Sousa, M. M.; Melo, MJ; A. J. Parola; de Melo, JSS; Catarino, F.; Pina, F; Cook, F. E. M.; Simmonds, M. S. J.; Lopes, J. A. (Journal of Chromatography A, 2008)
A simple and rapid liquid chromatographic method with diode-array UV-vis spectrophotometric detection has been developed for the authentication of dragon's blood resins from Dracaena and Daemonorops trees. Using this method it was discovered that the flavylium chromophores, which contribute to the red colour of these resins, differ among the species and could be used as markers to differentiate among species. A study of parameters, such as time of extraction, proportion of MeOH and pH, was undertaken to optimise the extraction of the flavyliums. This method was then used to make extracts from samples of dragon's blood resin obtained from material of known provenance. From the samples analysed 7,6-dihydroxy-5-methoxyflavylium (dracorhodin), 7,4'-dihydroxy-5-methoxyflavylium (dracoflavylium) and 7,4'-dihydroxyflavylium were selected as species markers for Daemonorops spp., Dracaena draco and Dracaena cinnabari, respectively. The chromatograms from these samples were used to build an HPLC-DAD database. The ability to discriminate among species of dragon's blood using the single marker compounds was compared with a principal components analysis of the chromatograms in the HPLC-DAD database. The results from the HPLC-DAD method based on the presence of these flavylium markers was unequivocal. The HPLC-DAD method was subsequently applied to 37 samples of dragon blood resins from the historical samples in the Economic Botany Collection, Royal Botanic Gardens, Kew. The method identified anomalies in how samples in this collection had been labelled. It is clear that the method can be used to evaluate the provenance of samples used in different areas of cultural heritage. It also could be used to monitor the trade of endangered species of dragon's blood and the species being used in complex formulations of traditional Chinese medicine. (C) 2008 Elsevier B.V. All rights reserved.
ISI Document Delivery No.: 372ZTTimes Cited: 14 Cited Reference Count: 36 Sousa, Micaela M. Melo, Maria J. Parola, A. Jorge Seixas de Melo, J. Sergio Catarino, Fernando Pina, Fernando Cook, Frances E. M. Simmonds, Monique S. J. Lopes, Joao A. Melo, Maria Joao/C-8594-2011; Seixas de Melo, Sergio/A-5388-2008; Lopes, Joao/H-1733-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Seixas de Melo, Sergio/0000-0001-9708-5079; Lopes, Joao/0000-0002-1657-344X; Parola, A. Jorge/0000-0002-1333-9076; Poci [poci/qui/55672/2004, ptdc/eat/65445/2006]; fct; feder We are grateful to POCI (POCI/QUI/55672/2004 and PTDC/EAT/65445/2006), FCT and FEDER for further funding. We would like to thank to botanical garden of Lisbon (Portugal), botanical garden of Ajuda (Portugal), to Roberto Jardim, director of botanical garden of Madeira (Portugal), to Natural Park of Madeira (Portugal) for the Dracaena draco, samples and J. Pavlis for the D. cinnabar! samples (Mendel University of Agriculture and Forestry, Czech Republic). We also would like to thank to Dr. Anita Quye (National Museums of Scotland, UK) for dragon's blood samples and Ms. H. Chantre (University of Coimbra, Portugal) for the Cape Verde species. Finally we would like to thank Prof. Joaquirn Marcalo (Instituto Tecnologico Nuclear, Portugal) for help in the MS measurements. Elsevier science bv Amsterdam

Glass weathering in eighteenth century mosaics: The São João Chapel in the São Roque Church in Lisbon - Salerno, C.S.; Moretti, C.; Medici, T.; Morna, T.; Verità, M. (Journal of Cultural Heritage, 2008)
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Cited By :3Export Date: 5 June 2018

Helical Twisting of Electrospun Liquid Crystalline Cellulose Micro- and Nanofibers - Canejo, João P; Borges, João P.; Godinho, M Helena; Brogueira, Pedro; Teixeira, Paulo IC; Terentjev, Eugene M (Advanced MaterialsAdvanced Materials, 2008)
Helically twisted fibers can be produced by electrospinning liquid-crystalline cellulose solutions. Fiber topographies are studied by atomic force microscopy, scanning electron microscopy (see figure) and polarized optical microscopy. The fibers have a nearly universal pitch-to-diameter ratio and comprise both right- and left-handed helices.
doi: 10.1002/adma.200801008
Keywords: Cellulose; chirality; Electrospinning; helices; liquid crystals; modelling; nanofibers

Ion jelly: a tailor-made conducting material for smart electrochemical devices - P. Vidinha; Lourenco, N. M. T.; Pinheiro, C; Bras, A. R.; T. Carvalho; Santos-Silva, T.; Mukhopadhyay, A.; Romao, M. J.; Parola, J.; Dionisio, M.; Cabral, J. M. S.; C. A. M. Afonso; et al. (Chemical Communications, 2008)
We present a new concept for the design of a polymeric conducting material that combines the chemical versatility of an organic salt ( ionic liquid) with the morphological versatility of a biopolymer ( gelatin); the resulting 'ion jelly' can be applied in electrochemical devices, such as batteries, fuel cells, electrochromic windows or photovoltaic cells.
ISI Document Delivery No.: 372GJTimes Cited: 16 Cited Reference Count: 27 Vidinha, Pedro Lourenco, Nuno M. T. Pinheiro, Carlos Bras, Ana R. Carvalho, Tania Santos-Silva, Teresa Mukhopadhyay, Abhik Romao, Maria J. Parola, Jorge Dionisio, Madalena Cabral, Joaquim M. S. Afonso, Carlos A. M. Barreiros, Susana Cabral, Joaquim /G-2052-2010; Barreiros, Susana/A-7892-2012; Lourenco, Nuno/K-5088-2012; Romao, Maria/A-4115-2013; Dionisio, Madalena/D-1946-2013; Mukhopadhyay, Abhik/D-2119-2013; Parola, A. Jorge/F-4048-2010; vidinha, Pedro/H-3426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Romao, Maria/0000-0002-3004-0543; Mukhopadhyay, Abhik/0000-0002-7755-0401; Parola, A. Jorge/0000-0002-1333-9076; vidinha, Pedro/0000-0002-3907-4969; Fundacao para a Ciencia e Tecnologia (FCT, Portugal) [PPCDT/BIO/57193/2004, POCI/QUI/57735/2004, SFRH/BD/18487/2004, SFRH/BD/2003] This work has been supported by Fundacao para a Ciencia e Tecnologia (FCT, Portugal) through research contracts PPCDT/BIO/57193/2004, POCI/QUI/57735/2004 and the grants SFRH/BD/18487/2004 (N. L.) and SFRH/BD/2003 ( P. V.), and by FEDER. We thank Dr Carla Rodrigues for DSC measurements. We thank Solchemar for the gift of ILs. Royal soc chemistry Cambridge

Mechanical characterization of friction stir welds of two dissimilar aluminium alloys of the 6xxx series - Moreira, P.M.G.P.; Santos, T.; Tavares, S.M.O.; Richter-Trummer, V.; Vilaça, P.; de Castro, P.M.S.T. (Advanced Materials Forum IV - Selected, peer reviewed papers from the 4th International Materials Symposium Materiais 2007 and 8th Encontro da Sociedade Portuguesa de Materiais - SPM, 2008)
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Conference code: 75401Cited By :4Export Date: 27 February 2015CODEN: MSFOE

Non destructive testing of Friction Stir Welding: Comparison of planar eddy current probes - Dos Santos, T.G.; Ramos, P.M.; Dos Santos Vilaça, P. (16th IMEKO TC4 International Symposium on Exploring New Frontiers of Instrumentation and Methods for Electrical and Electronic Measurements; 13th International Workshop on ADC Modelling and Testing - IMEKO TC4 - TC21 Joint Session, 2008)
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Conference code: 98878Export Date: 27 February 2015

A photochemical study on the blue dye indigo: from solution to ancient Andean textiles - Sousa, M. M.; Miguel, C.; Rodrigues, I.; A. J. Parola; Pina, F; de Melo, JSS; Melo, MJ (Photochemical & Photobiological Sciences, 2008)
The degradation of indigo and its water soluble derivative indigo carmine was investigated under light excitation in the presence and absence of molecular oxygen in solution (homogeneous) and gels (heterogeneous) media. Collagen and carboxymethylcellulose (CMC) aqueous gels were chosen to simulate a natural textile environment, wool and cotton, respectively. Isatin was found to be the major degradation product of indigo. In solution, the photodegradation quantum yields (Phi(R)) were in the order of 10(-4), with the exception of aqueous media (Phi(R) = 9 x 10(-6)), and dependent on the irradiation wavelength. In the case of indigo carmine the Phi(R) values were found to suffer a 2-fold increase upon going from water to gels. The results indicate the absence of degradation products involving singlet oxygen and suggest peroxides, or other oxygen based radicals, to have a key role in the degradation of indigo. Finally, the relevance of the simulation is discussed by comparing the main degradation products to those found in the blues of millenary Andean textiles.
ISI Document Delivery No.: 384GQTimes Cited: 10 Cited Reference Count: 37 Sousa, Micaela M. Miguel, Catarina Rodrigues, Isa Parola, A. Jorge Pina, Fernando Seixas de Melo, J. Sergio Melo, Maria J. Melo, Maria Joao/C-8594-2011; Seixas de Melo, Sergio/A-5388-2008; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Seixas de Melo, Sergio/0000-0001-9708-5079; Parola, A. Jorge/0000-0002-1333-9076; Portuguese Science Foundation; FEDER; Associate Laboratory for Green Chemistry [POCI/QUI/55672/2004] This work was supported by the Portuguese Science Foundation and FEDER, through the Associate Laboratory for Green Chemistry and the projects POCI/QUI/55672/2004 "The Molecules of Color in Art: a Photochemical Study". Meredith Montague and Richard Newman, at the Fine Arts Museum Boston, are kindly acknowledged for their collaboration in the Andean colors' project. The authors also thank Maria Manuel Marques, who kindly provided the purified DMF. Royal soc chemistry Cambridge

A Study in Mauve: Unveiling Perkin's Dye in Historic Samples - Sousa, M. M.; Melo, MJ; A. J. Parola; Morris, P. J. T.; Rzepa, H. S.; de Melo, JSS (Chemistry-a European Journal, 2008)
The analysis of different historic mauve samples-mauve salts and dyed textiles-was undertaken to establish the exact nature of the iconic dye produced by W.H. Perkin in the nineteenth century. Fourteen samples from important museum collections were analyzed, and it was determined that. in contrast to the general wisdom that mauveine consists of C, and C, structures. Perkin's mauveine is a complex mixture of at least thirteen methyl derivatives (C, to C(28)) with a 7-amino5-phenyl-3-(phenylamino)phenazin-5- ium core. A fingerprint was established in which mauveines A or B were dominant, and in which mauveines B2 and C(25) were found to be important tracers to probe the original synthesis. Counterion analysis showed that all the mauve salts should be dated after 1862. Perkin's original recipe could be identified in three textile samples, and in these cases, mauveines A and C25 were found to he the major chromophores. These are now shown to be the samples containing the "original mauve".
ISI Document Delivery No.: 360CZTimes Cited: 12 Cited Reference Count: 23 Sousa, Micaela M. Melo, Maria J. Parola, A. Jorge Morris, Peter J. T. Rzepa, Henry S. Seixas de Melo, J. Sergio Melo, Maria Joao/C-8594-2011; Seixas de Melo, Sergio/A-5388-2008; Rzepa, Henry/F-8979-2012; Parola, A. Jorge/F-4048-2010; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013 Seixas de Melo, Sergio/0000-0001-9708-5079; Parola, A. Jorge/0000-0002-1333-9076; Poci [poci/qui/55672/2004, ptdc/eat/65445/2006]; fct; feder We are grateful to POCI (POCI/QUI/55672/2004 and PTDC/EAT/65445/ 2006), FCT and FEDER for further funding, We also acknowledge Perth Museum (Scotland), Museum of Science and Industry in Manchester, Columbia University, New York City, and the Science Museum. London, for the samples, A. Travis for comments and to J. Marcalo (ITN) and M. da Conceicao (IST) Oliveira for help with MS measurements. Wiley-v c h verlag gmbh Weinheim

Use of fluorescence spectroscopy to study polymeric materials with porous structure based on imprinting by self-assembled fibrillar networks - Burguete, M. I.; Galindo, F; Gavara, R.; Izquierdo, M. A.; J. C. Lima; Luis, SV; A. J. Parola; Pina, F (Langmuir, 2008)
Different polymeric materials have been prepared from the oroartopels formed by a polymerizable methacrylic mixture (methyl i-nethacrylate/ethylene glycol dimethacrylate, 1: 1, w/w) and the macrocyclic pseudopeptide 1. The use of (2,4,6-trimethylbenzoyl)diphenylphosphine oxide as it very efficient radical initiator allows polymeric materials in which the structure of the fibrils formed by self-assembly of the organogelator 1 is truly preserved to be obtained. Removal of the pseudopeptidic molecule provides materials with a porous structure reflecting that of the original self-assembled fibrils. The use of fluorescent probes Such as rhodamine B and pyrene greatly facilitate the Study of the porous structures formed and, accordingly, that of the morphology of the original fibrils. Those studies reveal the presence of a permanent porosity and the organization of the Substructures as a porous network. This confirms the existence of a nucleation and growth mechanism for the generation of the fibrils, giving rise to the formation of spherulitic structures. Those spherulites are additionally linked by connections of variable size. A series of diffusion experiments allowed establishment of a direct dependence of the inner porosity of the materials oil the amount of self-organizing template used for their preparation.
ISI Document Delivery No.: 341NVTimes Cited: 20 Cited Reference Count: 89 Burguete, M. Isabel Galindo, Francisco Gavara, Raquel Izquierdo, M. Angeles Lima, Joao C. Luis, Santiago V. Parola, A. Jorge Pina, Fernando Pina, Fernando/C-8161-2011; Galindo, Francisco/H-8836-2012; Parola, A. Jorge/F-4048-2010; Gavara, Raquel/C-6669-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; Lima, Joao/F-3658-2010 Parola, A. Jorge/0000-0002-1333-9076; Lima, Joao/0000-0003-0528-1967 Spanish MEC [CTQ2006-15672-C05-02]; GVA [GV/2007/277, ARVIV/2007/079, ARVIV/2007/081]; Fundacio Caixa Castello-UJI [P1.1B2004-13]; MEC Financial support from the Spanish MEC (Grant CTQ2006-15672-C05-02), GVA (Projects GV/2007/277, ARVIV/2007/079, and ARVIV/2007/081), Fundacio Caixa Castello-UJI (Project P1.1B2004-13), and Fundacao para a Cienciae Tecnologia is acknowledged. F.G. and R.G. also thank the MEC for financial support (Ramon y Cajal program for F.G. and predoctoral FPI fellowship for R.G.). We also appreciate the helpful comments made by the reviewers of this work. Amer chemical soc Washington

Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis - Inácio, José Manuel; Correia, Isabel Lopes; Isabel de Sá-Nogueira (Microbiology, 2008)
Bacillus subtilis produces α-L-arabinofuranosidases (EC 3.2.1.55; AFs) capable of releasing arabinosyl oligomers and L-arabinose from plant cell walls. Here, we show by insertion-deletion mutational analysis that genes abfA and xsa(asd), herein renamed abf2, encode AFs responsible for the majority of the intracellular AF activity in B. subtilis. Both enzyme activities were shown to be cytosolic and functional studies indicated that arabino-oligomers are natural substrates for the AFs. The products of the two genes were overproduced in Escherichia coli, purified and characterized. The molecular mass of the purified AbfA and Abf2 was about 58 kDa and 57 kDa, respectively. However, native PAGE gradient gel analysis and cross-linking assays detected higher-order structures (>250 kDa), suggesting a multimeric organization of both enzymes. Kinetic experiments at 37°C, with p-nitrophenyl-α-L-arabinofuranoside as substrate, gave an apparent Km of 0.498 mM and 0.421 mM, and Vmax of 317 U mg−1 and 311 U mg−1 for AbfA and Abf2, respectively. The two enzymes displayed maximum activity at 50°C and 60°C, respectively, and both proteins were most active at pH 8.0. AbfA and Abf2 both belong to family 51 of the glycoside hydrolases but have different substrate specificity. AbfA acts preferentially on (1→5) linkages of linear α-1,5-L-arabinan and α-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan. In contrast, Abf2 is most active on (1→2) and (1→3) linkages of branched arabinan and arabinoxylan, suggesting a concerted contribution of these enzymes to optimal utilization of arabinose-containing polysaccharides by B. subtilis.
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Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-α-arabinanase from Bacillus subtilis - de Sanctis, Daniele; Bento, Isabel; Inácio, José Manuel; Custódio, Sónia; Isabel de Sá-Nogueira; Carrondo, Maria Arménia (Acta Crystallographica Section F, 2008)
Two Bacillus subtilis extracellular endo-1,5-α-L-arabinanases, AbnA and Abn2, belonging to glycoside hydrolase family 43 have been identified. The recently characterized Abn2 protein hydrolyzes arabinan and has low identity to other reported 1,5-α-L-arabinanases. Abn2 and its selenomethionine (SeMet) derivative have been purified and crystallized. Crystals appeared in two different space groups: P1, with unit-cell parameters a = 51.9, b = 57.6, c = 86.2 Å, α = 82.3, β = 87.9, ɣ = 63.6°, and P212121, with unit-cell parameters a = 57.9, b = 163.3, c = 202.0 Å. X-ray data have been collected for the native and the SeMet derivative to 1.9 and 2.7 Å resolution, respectively. An initial model of Abn2 is being built in the SeMet-phased map.
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Characterization of abn2 (yxiA), Encoding a Bacillus subtilis GH43 Arabinanase, Abn2, and Its Role in Arabino-Polysaccharide Degradation - Inácio, José Manuel; Isabel de Sá-Nogueira (Journal of Bacteriology, 2008)
The extracellular depolymerization of arabinopolysaccharides by microorganisms is accomplished by arabinanases, xylanases, and galactanases. Here, we characterize a novel endo-α-1,5-L-arabinanase (EC 3.2.1.99) from Bacillus subtilis, encoded by the yxiA gene (herein renamed abn2) that contributes to arabinan degradation. Functional studies by mutational analysis showed that Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in B. subtilis. Abn2 was overproduced in Escherichia coli, purified from the periplasmic fraction, and characterized with respect to substrate specificity and biochemical and physical properties. With linear-α-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent Km of 2.0 mg ml−1 and Vmax of 0.25 mmol min−1 mg−1 at pH 7.0 and 50°C. RNA studies revealed the monocistronic nature of abn2. Two potential transcriptional start sites were identified by primer extension analysis, and both a σA-dependent and a σH-dependent promoter were located. Transcriptional fusion studies revealed that the expression of abn2 is stimulated by arabinan and pectin and repressed by glucose; however, arabinose is not the natural inducer. Additionally, trans-acting factors and cis elements involved in transcription were investigated. Abn2 displayed a control mechanism at a level of gene expression different from that observed with AbnA. These distinct regulatory mechanisms exhibited by two members of extracellular glycoside hydrolase family 43 (GH43) suggest an adaptative strategy of B. subtilis for optimal degradation of arabinopolysaccharides.
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Adsorption of gum Arabic on bioceramic nanoparticles - Roque, Ana Cecília A; Wilson, O. C. (Materials Science & Engineering C.- Biomimetic and Supramolecular Systems, 2008)
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Analysis of the dynamics and origin of epileptic activity in patients with tuberous sclerosis evaluated for surgery of epilepsy - Alberto Leal; Ana I Dias; José P Vieira; Ana Moreira,; Luis Távora; Eulália Calado (Clinical Neurophysiology, 2008)

Analysis of the dynamics and origin of epileptic activity in patients with tuberous sclerosis evaluated for surgery of epilepsy - Leal, A; Dias, AI; Vieira, JP; Ana, Moreira; Távora, L; Calado, E (Clinical Neurophysiology, 2008)

Chloroquine mediated modulation of Anopheles gambiae gene expression - Abrantes, Patrícia; Dimopoulos, George; Ana Rita Grosso; do Rosário, Virgílio E; Silveira, Henrique (PloS one, 2008)
BACKGROUND: Plasmodium development in the mosquito is crucial for malaria transmission and depends on the parasite's interaction with a variety of cell types and specific mosquito factors that have both positive and negative effects on infection. Whereas the defensive response of the mosquito contributes to a decrease in parasite numbers during these stages, some components of the blood meal are known to favor infection, potentiating the risk of increased transmission. The presence of the antimalarial drug chloroquine in the mosquito's blood meal has been associated with an increase in Plasmodium infectivity for the mosquito, which is possibly caused by chloroquine interfering with the capacity of the mosquito to defend against the infection. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we report a detailed survey of the Anopheles gambiae genes that are differentially regulated by the presence of chloroquine in the blood meal, using an A. gambiae cDNA microarray. The effect of chloroquine on transcript abundance was evaluated separately for non-infected and Plasmodium berghei-infected mosquitoes. Chloroquine was found to affect the abundance of transcripts that encode proteins involved in a variety of processes, including immunity, apoptosis, cytoskeleton and the response to oxidative stress. This pattern of differential gene expression may explain the weakened mosquito defense response which accounts for the increased infectivity observed in chloroquine-treated mosquitoes. CONCLUSIONS/SIGNIFICANCE: The results of the present study suggest that chloroquine can interfere with several putative mosquito mechanisms of defense against Plasmodium at the level of gene expression and highlight the need for a better understanding of the impacts of antimalarial agents on parasite transmission.
Keywords: Animals; Anopheles gambiae; Anopheles gambiae: genetics; Antimalarials; Antimalarials: pharmacology; Apoptosis; Chloroquine; Chloroquine: pharmacology; Gene Expression; Gene Expression: drug effects; Genetic; Malaria; Malaria: microbiology; Oligonucleotide Array Sequence Analysis; Plasmodium berghei; Plasmodium berghei: pathogenicity; Transcription

Confinement and Surface Effects on the Molecular Dynamics of a Nematic Mixture Investigated by Dielectric Relaxation Spectroscop - A. R. Brás; M. Dionísio; A. Schoenhals (The Journal of Physical Chemistry B, 2008)

Cost of energy and environmental policy in Portuguese CO2 abatement—scenario analysis to 2020 - Simões, Sofia; Joao Cleto; Patrici Fortes; Júlia Seixas; Huppes, Gjalt (Energy Policy, 2008)
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Keywords: co 2 marginal abatement; cost; portuguese energy policies

The EMD and its use to identify system modes - Rato, R.T.; Ortigueira, M.; Batista, A. (International Workshop on New Trends in Science and Technology (NTST2008), 2008)
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The EMD and its Use to Identify System Modes - Rato, R.T.; Ortigueira, M.D.; Batista, A.G. (Proceedings of the International Workshop on New Trends in Science and Technology, 2008)
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The emerging role of splicing factors in cancer - Ana Rita Grosso; Martins, Sandra; Carmo-fonseca, Maria (EMBO Rep, 2008)
Recent progress in global sequence and microarray data analysis has revealed the increasing complexity of the human transcriptome. Alternative splicing generates a huge diversity of transcript variants and disruption of splicing regulatory networks is emerging as an important contributor to various diseases, including cancer. Current efforts to establish the dynamic repertoire of transcripts that are generated in health and disease are showing that many cancer-associated alternative-splicing events occur in the absence of mutations in the affected genes. A growing body of evidence reveals changes in splicing-factor expression that correlate with cancer development, progression and response to therapy. Here, we discuss how recent links between cancer and altered expression of proteins implicated in splicing regulation are bringing the splicing machinery to the fore as a potential target for anticancer treatment.
Keywords: 10; 1038; 1087; 1093; 189; 2008; 9; Cancer; doi; embo reports; embo reports advance online; embor; pre-mrna splicing; publication 10 october 2008; see glossary for abbreviations; spliceosome; splicing factor; therapeutic target; used in this article

The EmpiricalMode Decomposition: A Useful Tool for Signal Analysis. - Rato, R.T.; Ortigueira, M.D.; Batista, A.G. (New Signal Processing Research, 2008)
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