Estudo do efeito da adição de iões metálicos na corrosão de vidros - Vilarigues, Márcia (2008)
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Fractional Controller Design Trough Multi-Objective Optimization - Coito, F.; Ortigueira, M. (8th Portuguese Conference on Automatic Control ? CONTROLO?2008, 2008)
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The Fractional Quantum Derivative and the Fractional Linear Scale Invariant Systems - Ortigueira, M. (3rd IFAC Workshop on Fractional Differentiation and its Applications, 2008)
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The Fractional Quantum Derivative and the Generalised Euler-Cauchy Equation - Ortigueira, M. (2nd Conference on Nonlinear Science and Complexity, NSC08, 2008)
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Genome-scale classification of metabolic reactions and assignment of EC numbers with self-organizing maps - D. A. R. S. Latino; Q.-Y. Zhang; J. Aires-de-Sousa (Bioinformatics, 2008)

Helical Twisting of Electrospun Liquid Crystalline Cellulose Micro-and Nanofibers - Canejo, João Paulo; Borges, João Paulo; Godinho, Maria Helena; Brogueira, Pedro; Teixeira, Paulo IC; Terentjev, Eugene M (Advanced Materials, 2008)
Helically twisted fibers can be produced by electrospinning liquid-crystalline cellulose solutions. Fiber topographies are studied by atomic force microscopy, scanning electron microscopy (see figure) and polarized optical microscopy. The fibers have a nearly universal pitch-to-diameter ratio and comprise both right- and left-handed helices.

Identifying a Transfer Function From a Frequency Response - Ortigueira, M.D.; Valerio, D; da Costa, J.S. (Volume 5: 6th International Conference on Multibody Systems, Nonlinear Dynamics, and Control, Parts A, B, and C. :1405–1414, 2008)
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Influence of Crystallinity in Molecular Motions of Poly(L-lactic acid) Investigated by Dielectric Relaxation Spectroscopy - A. R. Brás; P. Malik; M. Dionísio; J.F. Mano (Macromolecules, 2008)

The Initial Conditions of Riemann-Liouville and Caputo Derivatives - Ortigueira, M.; Coito, F. (6th EUROMECH Conference ENOC 2008, 2008)
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Initial Conditions: What Are We Talking About? - Ortigueira, M.; Coito, F. (3rd IFAC Workshop on Fractional Differentiation and its Applications, 2008)
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Isotopic labeling of c-type multiheme cytochromes overexpressed in E. coli - Fernandes, Ana P.; Isabel Couto; Leonor Morgado; Londer, Yuri Y.; Salgueiro, C.A. (Protein Expression and Purification, 2008)
Progresses made in bacterial genome sequencing show a remarkable profusion of multiheme c-type cytochromes in many bacteria, highlighting the importance of these proteins in different cellular events. However, the characterization of multiheme cytochromes has been significantly retarded by the numerous experimental challenges encountered by researchers who attempt to overexpress these proteins, especially if isotopic labeling is required. Here we describe a methodology for isotopic labeling of multiheme cytochromes c overexpressed in Escherichia coli, using the triheme cytochrome PpcA from Geobacter sulfurreducens as a model protein. By combining different strategies previously described and using E. coli cells containing the gene coding for PpcA and the cytochrome c maturation gene cluster, an experimental labeling methodology was developed that is based on two major aspects: (i) use of a two-step culture growth procedure, where cell growth in rich media was followed by transfer to minimal media containing 15N-labeled ammonium chloride, and (ii) incorporation of the heme precursor delta-aminolevulinic acid in minimal culture media. The yields of labeled protein obtained were comparable to those obtained for expression of PpcA in rich media. Proper protein folding and labeling were confirmed by UV–visible and NMR spectroscopy. To our knowledge, this is the first report of a recombinant multiheme cytochrome labeling and it represents a major breakthrough for functional and structural studies of multiheme cytochromes.

Luminescent Glasses in Art - Teresa Almeida; Ruivo, Andreia; António Pires de Matos; Rosa Maria de Oliveira; Aquino Antunes (Journal of Cultural Heritage, 2008)

Molecular Motions in Amorphous Ibuprofen As Studied by Broadband Dielectric Spectroscopy - A. R. Brás; J. P. Noronha; A.M.M. Antunes; M. M. Cardoso; A. Schoenhals; F. Affouard; M. Dionísio; N. T. Correia (The Journal of Physical Chemistry B, 2008)

Novel Unsaturated Sucrose Ethers and Their Application as Monomers - C.C. Crucho; K. T. Petrova; R.C. Pinto; M. T. Barros (Molecules, 2008)

Origin of frontal lobe spikes in the early onset benign occipital lobe epilepsy (Panayiotopoulos syndrome) - Alberto Leal; José C Ferreira; Ana I Dias; Eulália Calado (Clinical Neurophysiology, 2008)

Parecer sobre a Instalação de Tratamento Físico-Químico e Armazenamento Temporário de Resíduos Industriais da Quimitécnica Ambiente, - Amado, Miguel (PROGESTO, 2008)

Peptidoglycan Recognition Protein-SD provides versatility of receptor formation in Drosophila immunity - L. Wang; R. J. C. Gilbert; M. L. Atilano; S. R. Filipe; N. J. Gay; P. Ligoxygakis (Proc. Natl. Acad. Sci. USA, 2008)

Redes Neuronais em Termodinâmica Estatística e na Classificação Automática de Reacções Químicas - D. A. R. S. Latino (FCT/UNL, 2008)

A remote laboratory environment for blended learning - Coito, F.; Palma, L. B. (Proceedings of the 1st international conference on PErvasive Technologies Related to Assistive Environments. :69.: ACM, 2008)
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The Solution Structure of a Tetraheme Cytochrome from Shewanella frigidimarina Reveals a Novel Family Structural Motif - Paixão, Vitor B.; Salgueiro, C.A.; Brennan, Lorraine; Graeme A. Reid; Stephen K. Chapman; Turner, David L. (Biochemistry, 2008)
The bacteria belonging to the genus Shewanella are facultative anaerobes that utilize a variety of terminal electron acceptors which includes soluble and insoluble metal oxides. The tetraheme c-type cytochrome isolated during anaerobic growth of Shewanella frigidimarina NCIMB400 (Sfc) contains 86 residues and is involved in the Fe(III) reduction pathways. Although the functional properties of Sfc redox centers are quite well described, no structures are available for this protein. In this work, we report the solution structure of the reduced form of Sfc. The overall fold is completely different from those of the tetraheme cytochromes c3 and instead has similarities with the tetraheme cytochrome recently isolated from Shewanella oneidensis (Soc). Comparison of the tetraheme cytochromes from Shewanella shows a considerable diversity in their primary structure and heme reduction potentials, yet they have highly conserved heme geometry, as is the case for the family of tetraheme cytochromes isolated from Desulfovibrio spp.

Stained glass from Monastery of Batalha: non-destructive characterization of glass and paintings - Fernandes, P.; Vilarigues, M.; L.C. Alves; R.C. da Silva (Journal of Cultural Heritage, 2008)

Staphylococcus aureus PBP4 is essential for beta-lactams resistance in community-acquired methicillin resistant strains - G. Memmi; S. R. Filipe; M. G. Pinho; Z. Fu; A. Cheung (Antimicrob Agents Chemother, 2008)

Structural insights into the modulation of the redox properties of two Geobacter sulfurreducens homologous triheme cytochromes - Leonor Morgado; Bruix, Marta; Valerie Orshonsky; Londer, Yuri Y.; Norma E.C. Duke; Xiaojing Yang; Pokkuluri, P.R.; Schiffer, Marianne; Salgueiro, C.A. (Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2008)
The redox properties of a periplasmic triheme cytochrome, PpcB from Geobacter sulfurreducens, were studied by NMR and visible spectroscopy. The structure of PpcB was determined by X-ray diffraction. PpcB is homologous to PpcA (77% sequence identity), which mediates cytoplasmic electron transfer to extracellular acceptors and is crucial in the bioenergetic metabolism of Geobacter spp. The heme core structure of PpcB in solution, probed by 2D-NMR, was compared to that of PpcA. The results showed that the heme core structures of PpcB and PpcA in solution are similar, in contrast to their crystal structures where the heme cores of the two proteins differ from each other. NMR redox titrations were carried out for both proteins and the order of oxidation of the heme groups was determined. The microscopic properties of PpcB and PpcA redox centers showed important differences: (i) the order in which hemes become oxidized is III–I–IV for PpcB, as opposed to I–IV–III for PpcA; (ii) the redox-Bohr effect is also different in the two proteins. The different redox features observed between PpcB and PpcA suggest that each protein uniquely modulates the properties of their co-factors to assure effectiveness in their respective metabolic pathways. The origins of the observed differences are discussed.

Structures and Solution Properties of Two Novel Periplasmic Sensor Domains with c-Type Heme from Chemotaxis Proteins of Geobacter sulfurreducens: Implications for Signal Transduction - Pokkuluri, P.R.; Pessanha, M.; Londer, Y.Y.; Wood, S.J.; Duke, N.E.C.; Wilton , R.; Catarino, T.; Salgueiro, C.A.; Schiffer, M. (Journal of Molecular Biology, 2008)
Periplasmic sensor domains from two methyl-accepting chemotaxis proteins from Geobacter sulfurreducens (encoded by genes GSU0935 and GSU0582) were expressed in Escherichia coli. The sensor domains were isolated, purified, characterized in solution, and their crystal structures were determined. In the crystal, both sensor domains form swapped dimers and show a PAS-type fold. The swapped segment consists of two helices of about 45 residues at the N terminus with the hemes located between the two monomers. In the case of the GSU0582 sensor, the dimer contains a crystallographic 2-fold symmetry and the heme is coordinated by an axial His and a water molecule. In the case of the GSU0935 sensor, the crystals contain a non-crystallographic dimer, and surprisingly, the coordination of the heme in each monomer is different; monomer A heme has His-Met ligation and monomer B heme has His-water ligation as found in the GSU0582 sensor. The structures of these sensor domains are the first structures of PAS domains containing covalently bound heme. Optical absorption, electron paramagnetic resonance and NMR spectroscopy have revealed that the heme groups of both sensor domains are high-spin and low-spin in the oxidized and reduced forms, respectively, and that the spin-state interconversion involves a heme axial ligand replacement. Both sensor domains bind NO in their ferric and ferrous forms but bind CO only in the reduced form. The binding of both NO and CO occurs via an axial ligand exchange process, and is fully reversible. The reduction potentials of the sensor domains differ by 95 mV (− 156 mV and − 251 mV for sensors GSU0582 and GSU0935, respectively). The swapped dimerization of these sensor domains and redox-linked ligand switch might be related to the mechanism of signal transduction by these chemotaxis proteins.

Tissue-specific splicing factor gene expression signatures - Ana Rita Grosso; Gomes, Anita Q; Barbosa-Morais, Nuno L.; Caldeira, Sandra; Thorne, Natalie P.; Grech, Godfrey; von Lindern, Marieke; Carmo-fonseca, Maria (Nucl. Acids Res., 2008)
The alternative splicing code that controls and coordinates the transcriptome in complex multicellular organisms remains poorly understood. It has long been argued that regulation of alternative splicing relies on combinatorial interactions between multiple proteins, and that tissue-specific splicing decisions most likely result from differences in the concentration and/or activity of these proteins. However, large-scale data to systematically address this issue have just recently started to become available. Here we show that splicing factor gene expression signatures can be identified that reflect cell type and tissue-specific patterns of alternative splicing. We used a computational approach to analyze microarray-based gene expression profiles of splicing factors from mouse, chimpanzee and human tissues. Our results show that brain and testis, the two tissues with highest levels of alternative splicing events, have the largest number of splicing factor genes that are most highly differentially expressed. We further identified SR protein kinases and small nuclear ribonucleoprotein particle (snRNP) proteins among the splicing factor genes that are most highly differentially expressed in a particular tissue. These results indicate the power of generating signature-based predictions as an initial computational approach into a global view of tissue-specific alternative splicing regulation.
Keywords: Alternative Splicing; analysis/metabolism; Animals; Cell Differentiatio; Cell Differentiation; Cell Differentiation: genetics; Cell Line; Computational Biology; Gene; Gene Expression Profiling; genetics; genetics/metabolism; Humans; Messenger; Messenger: analysis; Messenger: metabolism; Mice; Oligonucleotide Array Sequence Analysis; Pan troglodytes; Pan troglodytes: genetics; RNA; RNA-Binding Proteins; RNA-Binding Proteins: genetics; RNA-Binding Proteins: metabolism; Tissue Distribution

{Portugal e a economia do conhecimento: A despesa empresarial em Investigação e Desenvolvimento [Portugal and knowledge economy: the business R&D expenditures]} - Nuno Boavida (Universidade Nova de Lisboa, IET/CICS.NOVA-Interdisciplinary Centre on Social Sciences, Faculty of Science and Technology, 2008)
The objective of the paper is to contribute to the understanding of the knowledge economy in Portugal, presenting data and revealing specific characteristics of the Portuguese business R&D, using a framework of available tools such as international comparisons, technology intensity and sectors of the economy. This study concludes that Portuguese business R&D expenditures reveals strong elements of economic terciarization, and an important presence of foreign direct investment in knowledge intensive industries. Furthermore, the study revealed less significant determinants such as the existence of skill labour in pharmaceutical industries and the role of the state in some sectors (wealth and rail transportation).
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Keywords: business R&D; knowledge intensive industries; technology intensity

Anion detection by fluorescent Zn(II) complexes of functionalized polyamine ligands - Rodriguez, Laura; Lima, Joao C.; A. Jorge Parola; Pina, Fernando; Meitz, Robert; Aucejo, Ricardo; Garcia-Espana, Enrique; Llinares, Jose M.; Soriano, Conxa; Alarcon, Javier (Inorganic Chemistry, 2008)
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Biaxial nematic order and phase behavior studies in an organosiloxane tetrapode using complementary deuterium NMR experiments - C. Cruz; J. L. Figueirinhas; Filip, D.; Feio, G.; Ribeiro, A. C.; Frere, Y.; Meyer, T.; Mehl, G. H. (Phys Rev E Stat Nonlin Soft Matter Phys, 2008)
The biaxial nematic phase was recently observed in different thermotropic liquid crystals, namely bent-core compounds, side-chain polymers, bent-core dimers, and organosiloxane tetrapodes. In this work, a series of experiments with a nematic organosiloxane tetrapode where nuclear magnetic resonance (NMR) spectra are collected while the sample is continuously rotating around an axis perpendicular to the magnetic field, are discussed in conjunction with the analysis of a deuterium NMR experiment on the same system reported earlier. The sample used is a mixture of a deuterated probe with the tetrapode. The mixture exhibits a nematic range between -40 degrees C and 37 degrees C. The results of the two independent, but complementary deuterium NMR experiments confirm the existence of a biaxial nematic phase for temperatures below 0 degrees C with high values of the asymmetry parameter at low temperatures. The presence of slow movements of the tetrapode mesogenic units in the low-temperature regime could also be detected through the analysis of the NMR spectra. Simulations indicate that these movements are mainly slow molecular reorientations of the mesogenic units associated with the presence of collective modes in the nematic phases of this compound. In the case of tetrapodes, recent investigations attribute the origin of biaxiality to the hindering of reorientations of the laterally attached mesogenic units which constitute the tetrapode. This study relates the molecular movements with the nematic biaxial ordering of the system.
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Biologically relevant O,S-donor compounds. Synthesis, molybdenum complexation and xanthine oxidase inhibition - Chaves, Silvia; Gil, Marco; Canario, Sonia; Jelic, Ratomir; Romao, Maria Joao; Trincao, Jose; Herdtweck, Eberhardt; Sousa, Joana; Diniz, Carmen; Fresco, Paula; Santos, M. Amelia (Dalton Transactions, 2008)
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Times Cited: 9 Chaves, Silvia/H-9865-2012; Santos, M. Amelia /H-9409-2012; Romao, Maria/A-4115-2013; Fresco, Paula/D-4845-2013; Diniz, Carmen/D-6293-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Santos, M. Amelia /0000-0002-4069-9368; Romao, Maria/0000-0002-3004-0543; Fresco, Paula/0000-0001-6705-7798; Diniz, Carmen/0000-0003-4668-9360; 9

Capture of Dioxins by ionic liquids - Kulkarni, Prashant S.; Branco, Luis C.; Crespo, Joao G.; Afonso, Carlos A. M. (Environmental Science & Technology, 2008)
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Times Cited: 14 Branco, Luis/C-6426-2013; Crespo, Joao/D-8719-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 15

Critical fluctuations and random-anisotropy glass transition in nematic elastomers - Feio, G.; J. L. Figueirinhas; Tajbakhsh, A. R.; Terentjev, E. M. (Physical Review B, 2008)
We carry out a detailed deuterium nuclear magnetic resonance (NMR) study of local nematic ordering in polydomain nematic elastomers. This system has a close analogy to the random-anisotropy spin glass. We find that, in spite of the quadrupolar nematic symmetry in three dimensions requiring a first-order transition, the order parameter in the quenched "nematic glass" emerges via a continuous phase transition. In addition to this remarkable effect, by a careful analysis of the NMR line shape, we deduce that the local director fluctuations grow in a critical manner around the transition point. This could become an essential experimental evidence for the quenched disorder changing the order of discontinuous transition.
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Keywords: crystal elastomers impurities stability network order

Dealing with Complexity in Supervision Systems - Palma, L. B.; Gil, P.S.; Coito, F. V.; Duarte-Ramos, H. (2008)
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Deuterium NMR Investigation of the Influence of Molecular Structure on the Biaxial Ordering of Organosiloxane Tetrapodes Nematic Phase - Cardoso, M.; J. L. Figueirinhas; C. Cruz; Van-Quynh, A.; Ribeiro, A. C.; Feio, G.; Apreutesei, D.; Mehl, G. H. (Molecular Crystals and Liquid Crystals, 2008)
In order to contribute to the understanding of the origin of biaxial nematic ordering in tetrapodes, a deuterium NMR study was performed on mixtures of monomers from organosiloxane tetrapodes with a deuterated nematic probe. Contrary to the tetrapode system previously studied, which exhibits a biaxial nematic phase, the results for monomers are compatible, within the experimental error, with uniaxial nematic ordering in the whole nematic range. The data are in agreement with the conjecture that the nematic biaxial behaviour is related to hindering of the mesogenic units' rotational movements, arising from interdigitation and connection to the central silicon core.
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Keywords: biaxial nematic phase dendrimers deuterium nmr organosiloxane tetrapodes liquid-crystals

Effect of physicochemical conditions on the ultrafiltration of beta-lactoglobulin: Fluorescence probing of induced structural changes - Portugal, Carla A. M.; J. C. Lima; Crespo, Joao G. (Journal of Membrane Science, 2008)
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Electrocardiografia de Alta-Resolução: Evolução e Estado da Arte - Batista, A.G. (Sessões Temáticas em Cardiopneumologia, 2008)
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Electron transfer complex between nitrous oxide reductase and cytochrome c(552) from Pseudomonas nautica: Kinetic, nuclear magnetic resonance, and docking studies - Dell'Acqua, Simone; Pauleta, Sofia R.; Monzani, Enrico; Pereira, Alice S.; Casella, Luigi; Moura, Jose J. G.; Moura, Isabel (Biochemistry, 2008)
The multicopper enzyme nitrous oxide reductase (N2OR) catalyzes the final step of denitrification, the two-electron reduction of N2O to N-2. This enzyme is a functional homodimer containing two different multicopper sites: CuA and CuZ. CuA is a binuclear copper site that transfers electrons to the tetranuclear copper sulfide CuZ, the catalytic site. In this study, Pseudomonas nautica cytochrome C-552 was identified as the physiological electron donor. The kinetic data show differences when physiological and artificial electron donors are compared [cytochrome vs methylviologen (MV)]. In the presence of cytochrome c(552), the reaction rate is dependent on the ET reaction and independent of the N2O concentration. With MV, electron donation is faster than substrate reduction. From the study of cytochrome c(552) concentration dependence, we estimate the following kinetic parameters: K-mc512 = 50.2 +/- 9.0 mu M and V-maxc551 1.8 +/- 10.6 units/mg. The N2O concentration dependence indicates a K-mN2O of 14.0 +/- 2.9 mu M using MV as the electron donor. The pH effect on the kinetic parameters is different when MV or cytochrome c(552) is used as the electron donor (pK(a) = 6.6 or 8.3, respectively). The kinetic study also revealed the hydrophobic nature of the interaction, and direct electron transfer studies showed that CuA is the center that receives electrons from the physiological electron donor. The formation of the electron transfer complex was observed by H-1 NMR protein-protein titrations and was modeled with a molecular docking program (BiGGER). The proposed docked complexes corroborated the ET studies giving a large number of solutions in which cytochrome c(552) is placed near a hydrophobic patch located around the CuA center.
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The EMD and its use to identify system modes - Rato, R.T.; Ortigueira, M.; Arnaldo Batista (International Workshop on New Trends in Science and Technology (NTST2008), 2008)
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The EMD and its Use to Identify System Modes - Rato, R.T.; Ortigueira, M.D.; Batista, A.G. (Proceedings of the International Workshop on New Trends in Science and Technology, 2008)
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The Empirical Mode Decomposition: A Useful Tool for Signal Analysis - Rato, R.T.; Ortigueira, M.D.; Batista, A.G. (New Signal Processing Research, 2008)
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Exploration of quantitative structure-property relationships (QSPR) for the design of new guanidinium ionic liquids - Carrera, Goncalo V. S. M.; Branco, Luis C.; Aires-de-Sousa, Joao; Afonso, Carlos A. M. (Tetrahedron, 2008)
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Times Cited: 19 Branco, Luis/C-6426-2013; Carrrera, Goncalo/C-7536-2013; Aires-de-Sousa, Joao/C-7826-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, ORG/M-4578-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Aires-de-Sousa, Joao/0000-0002-5887-2966; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 19

A Fault Tolerant Approach for Networked Control Systems Using Predictive Models - Palma, L.; Coito, F.; Neves-Silva, R.; Gil, P. (2008)
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Flavylium chromophores as species markers for dragon's blood resins from Dracaena and Daemonorops trees - Sousa, Micaela M.; Melo, Maria J.; A. Jorge Parola; Seixas de Melo, J. Sergio; Catarino, Fernando; Pina, Fernando; Cook, Frances E. M.; Simmonds, Monique S. J.; Lopes, Joao A. (Journal of Chromatography A, 2008)
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Times Cited: 16 Melo, Maria Joao/C-8594-2011; Seixas de Melo, Sergio/A-5388-2008; Lopes, Joao/H-1733-2011; Pina, Fernando/C-8161-2011; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; Group, Photochem&SC/M-4665-2013; REQUIMTE, ANALYSIS/M-4644-2013; Parola, A. Jorge/F-4048-2010; REQUIMTE, LAQV/N-9835-2013 Melo, Maria Joao/0000-0001-7393-6801; Seixas de Melo, Sergio/0000-0001-9708-5079; Lopes, Joao/0000-0002-1657-344X; Pina, Fernando/0000-0001-8529-6848; Parola, A. Jorge/0000-0002-1333-9076; 18

Fractional Central Differences and Derivatives - Ortigueira, M.D. (Journal of Vibration and Control, 2008)
Fractional central differences and derivatives are studied in this article. These are generalisations to real orders of the ordinary positive (even and odd) integer order differences and derivatives, and also coincide with the well known Riesz potentials. The coherence of these definitions is studied by applying the definitions to functions with Fourier transformable functions. Some properties of these derivatives are presented and particular cases studied.
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Fractional Controller Design Trough Multi-Objective Optimization - Fernando Coito; Manuel Ortigueira (8th Portuguese Conference on Automatic Control ? CONTROLO?2008, 2008)
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Fractional Controller Design Trough Multi-Objective Optimization - Coito, F.; Ortigueira, M. (2008)
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A fractional quantum derivative - Ortigueira, M. (3rd IFAC Workshop on Fractional Differentiation and its Applications, 2008)
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The Fractional Quantum Derivative and the Generalised Euler-Cauchy Equation - Manuel Ortigueira (2nd Conference on Nonlinear Science and Complexity, NSC08, 2008)
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Identifying a Transfer Function From a Frequency Response - Manuel Duarte Ortigueira; Duarte Vale?rio; Jose? Sa? da Costa (Volume 5: 6th International Conference on Multibody Systems, Nonlinear Dynamics, and Control, Parts A, B, and C, 2008)
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The Initial Conditions of Riemann-Liouville and Caputo Derivatives - Manuel Ortigueira; Fernando Coito (6th EUROMECH Conference ENOC 2008, 2008)
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The initial conditions of Riemann-Liouville and Caputo derivatives - Ortigueira, M.; Coito, F. (2008)
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Initial Conditions: What Are We Talking About? - Ortigueira, M.; Coito, F. (3rd IFAC Workshop on Fractional Differentiation and its Applications, 2008)
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Interaction of polyamine macrocycles with Zn(II) and ATP in aqueous solution. Binary and ternary systems. A potentiometric, NMR and fluorescence emission study - Bazzicalupi, Carla; Bencini, Andrea; Bianchi, Antonio; Danesi, Andrea; Faggi, Enrico; Giorgi, Claudia; Lodeiro, Carlos; Oliveira, Elisabete; Pina, Fernando; Valtancoli, Barbara (Inorganica Chimica Acta, 2008)
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Times Cited: 9 Pina, Fernando/C-8161-2011; Lodeiro, Carlos/B-4793-2013; Oliveira, Elisabete /C-7852-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, C4O/M-5599-2013; REQUIMTE, UCIBIO/N-9846-2013; REQUIMTE, LAQV/N-9835-2013 Pina, Fernando/0000-0001-8529-6848; Lodeiro, Carlos/0000-0001-5582-5446; 9

Interaction of zinc tetrasulfonated phthalocyanine with cytochrome c in water and Triton-X 100 micelles - Laia, Cesar A. T.; Costa, Silvia M. B. (Journal of Physical Chemistry B, 2008)
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Times Cited: 2 Laia, Cesar/C-8651-2011; Costa, Silvia/H-7766-2012; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; Costa, Silvia/M-8085-2013; REQUIMTE, LAQV/N-9835-2013 Laia, Cesar/0000-0001-6410-6072; Costa, Silvia/0000-0002-6978-8903; 2

An introduction to the fractional continuous-time linear systems: the 21st century systems - Ortigueira, M.D. (IEEE Circuits and Systems Magazine, 2008)
A brief introduction to the fractional continuous-time linear systems is presented. It will be done without needing a deep study of the fractional derivatives. We will show that the computation of the impulse and step responses is very similar to the classic. The main difference lies in the substitution of the exponential by the Mittag-Leffler function. We will present also the main formulae defining the fractional derivatives.
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Investigation of layered double hydroxides intercalated by oxomolybdenum catecholate complexes - Monteiro, Bernardo; Gago, Sandra; Paz, Filipe A. Almeida; Bilsborrow, Robert; Goncalves, Isabel S.; Pillinger, Martyn (Inorganic Chemistry, 2008)
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Times Cited: 7 Gago, Sandra/H-5413-2012; Monteiro, Bernardo/C-6940-2013; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; REQUIMTE, PHOTO/M-4229-2013; Almeida Paz, Filipe/F-3430-2010; REQUIMTE, LAQV/N-9835-2013 Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Almeida Paz, Filipe/0000-0003-2051-5645; 7

Ion jelly: a tailor-made conducting material for smart electrochemical devices - Vidinha, Pedro; Lourenco, Nuno M. T.; Pinheiro, Carlos; Bras, Ana R.; Carvalho, Tania; Santos-Silva, Teresa; Mukhopadhyay, Abhik; Romao, Maria J.; Parola, Jorge; Dionisio, Madalena; Cabral, Joaquim M. S.; Afonso, Carlos A. M.; et al. (Chemical Communications, 2008)
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Times Cited: 20 Cabral, Joaquim /G-2052-2010; Barreiros, Susana/A-7892-2012; Lourenco, Nuno/K-5088-2012; Romao, Maria/A-4115-2013; Dionisio, Madalena/D-1946-2013; Mukhopadhyay, Abhik/D-2119-2013; vidinha, Pedro/H-3426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, CAT/M-4526-2013; REQUIMTE, PHOTO/M-4229-2013; Group, Photochem&SC/M-4665-2013; REQUIMTE, CENG/M-4555-2013; REQUIMTE, SMB/M-5694-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; Parola, A. Jorge/F-4048-2010; REQUIMTE, NSF/N-1636-2013; REQUIMTE, UCIBIO/N-9846-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Cabral, Joaquim /0000-0002-2405-5845; Barreiros, Susana/0000-0002-7268-5892; Lourenco, Nuno/0000-0001-9329-2724; Romao, Maria/0000-0002-3004-0543; Dionisio, Madalena/0000-0002-1487-0889; Mukhopadhyay, Abhik/0000-0002-7755-0401; vidinha, Pedro/0000-0002-3907-4969; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; Parola, A. Jorge/0000-0002-1333-9076; 20

Ionic liquids as an efficient bulk membrane for the selective transport of organic compounds - Branco, Luis C.; Crespo, Joao G.; Afonso, Carlos A. M. (Journal of Physical Organic Chemistry, 2008)
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Times Cited: 9 Branco, Luis/C-6426-2013; Crespo, Joao/D-8719-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; iMed.ULisboa, BioOrgChem /B-4209-2014; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; Annual European Conference on Complex Systems Oct 01-06, 2007 Dresden, GERMANY 9

Light activation of transcription: photocaging of nucleotides for control over RNA polymerization - Pinheiro, Andre Vidal; Pedro Baptista; Lima, Joao Carlos (Nucleic Acids Research, 2008)
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Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - an NMR, X-ray crystallography and computational chemistry approach - Viegas, Aldino; Bras, Natercia F.; Cerqueira, Nuno M. F. S. A.; Fernandes, Pedro Alexandrino; Prates, Jose A. M.; Fontes, Carlos M. G. A.; Bruix, Marta; Romao, Maria Joao; Carvalho, Ana Luisa; Ramos, Maria Joao; Macedo, Anjos L.; Cabrita, Eurico J. (Febs Journal, 2008)
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Times Cited: 13 Cabrita, Eurico/E-8053-2011; Bruix, Marta/H-4161-2011; Cerqueira, Nuno M. F. Sousa A./A-5182-2008; Carvalho, Ana Luisa/G-5638-2011; Romao, Maria/A-4115-2013; Fernandes, Pedro/D-6327-2013; Bras, Natercia/D-5493-2013; Macedo, Anjos/D-7216-2013; Ramos, Maria/D-6183-2013; Viegas, Aldino/A-1889-2011; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; Prates, Jose/K-9934-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, TCB/M-6190-2013; REQUIMTE, UCIBIO/N-9846-2013 Cabrita, Eurico/0000-0002-0720-2751; Cerqueira, Nuno M. F. Sousa A./0000-0003-0342-7424; Carvalho, Ana Luisa/0000-0002-3824-0240; Romao, Maria/0000-0002-3004-0543; Fernandes, Pedro/0000-0003-2748-4722; Bras, Natercia/0000-0002-3130-9807; Macedo, Anjos/0000-0002-2613-4838; Ramos, Maria/0000-0002-7554-8324; Viegas, Aldino/0000-0003-1733-136X; Prates, Jose/0000-0003-1032-5987; 13

Multiplexed spectral coding for simultaneous detection of DNA hybridization reactions based on FRET - Leticia Giestas; Guilherme N.M. Ferreira; Pedro V. Baptista; Lima, Joao Carlos (Sensors and Actuators B-Chemical, 2008)
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Neutral gold(I) metallosupramolecular compounds: Synthesis and characterization, photophysical properties, and density functional theory studies - Rodriguez, Laura; Lodeiro, Carlos; Lima, Joao Carlos; Crehuet, Ramon (Inorganic Chemistry, 2008)
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A new type of metal-binding site in cobalt- and zinc-containing adenylate kinases isolated from sulfate-reducers Desulfovibrio gigas and Desulfovibrio desulfuricans ATCC 27774 - Gavel, Olga Yu; Bursakov, Sergey A.; Di Rocco, Giulia; Trincao, Jose; Pickering, Ingrid J.; George, Graham N.; Calvete, Juan J.; Shnyrov, Valery L.; Brondino, Carlos D.; Pereira, Alice S.; Lampreia, Jorge; Tavares, Pedro; et al. (Journal Of Inorganic Biochemistry, 2008)
Adenylate kinase (AK) mediates the reversible transfer of phosphate groups between the adenylate nucleotides and contributes to the maintenance of their constant cellular level, necessary for energy metabolism and nucleic acid synthesis. The AK were purified from crude extracts of two sulfate-reducing bacteria (SRB), Desulfovibrio (D.) gigas NCIB 9332 and Desulfovibrio desulfuricans ATCC 27774, and biochemically and spectroscopically characterised in the native and fully cobalt- or zinc-substituted forms. These are the first reported adenylate kinases that bind either zinc or cobalt and are related to the subgroup of metal-containing AK found, in most cases, in Gram-positive bacteria. The electronic absorption spectrum is consistent with tetrahedral coordinated cobalt, predominantly via sulfur ligands, and is supported by EPR. The involvement of three cysteines in cobalt or zinc coordination was confirmed by chemical methods. Extended X-ray absorption fine structure (EXAFS) indicate that cobalt or zinc are bound by three cysteine residues and one histidine in the metal-binding site of the ``LID'' domain. The sequence (129)Cys-X(5)-His-X(15)-Cys-X(2)-Cys of the AK from D. gigas is involved in metal coordination and represents a new type of binding motif that differs from other known zinc-binding sites of AK. Cobalt and zinc play a structural role in stabilizing the LID domain. (C) 2008 Elsevier Inc. All rights reserved.
13th International Conference on Biological Inorganic Chemistry, Vienna, AUSTRIA, JUL 15-20, 2007

On the HHT, its problems, and some solutions - Rato, R.T.; Ortigueira, M.D.; Batista, A.G. (Mechanical Systems and Signal Processing, 2008)
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On the relation between the fractional Brownian motion and the fractional derivatives - Ortigueira, M.D.; Batista, A.G. (Physics Letters, 2008)
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Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum - Najmudin, Shabir; Gonzalez, Pablo J.; Trincao, Jose; Coelho, Catarina; Mukhopadhyay, Abhik; Cerqueira, Nuno M. F. S. A.; Romao, Carlos C.; Moura, Isabel; Moura, Jose J. G.; Brondino, Carlos D.; Romao, Maria J. (Journal of Biological Inorganic Chemistry, 2008)
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Times Cited: 37 Romao, Carlos /D-1162-2010; Cerqueira, Nuno M. F. Sousa A./A-5182-2008; Romao, Maria/A-4115-2013; Coelho, Catarina /D-2073-2013; Mukhopadhyay, Abhik/D-2119-2013; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Gonzalez, Pablo/D-8200-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, TCB/M-6190-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Carlos /0000-0001-5061-3743; Cerqueira, Nuno M. F. Sousa A./0000-0003-0342-7424; Romao, Maria/0000-0002-3004-0543; Coelho, Catarina /0000-0003-1852-5406; Mukhopadhyay, Abhik/0000-0002-7755-0401; Moura, Isabel/0000-0003-0971-4977; 37

Photochromic soft materials: Flavylium compounds incorporated into pluronic F-127 hydrogel matrixes - Pina, Fernando; Hatton, T. Alan (Langmuir, 2008)
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Times Cited: 16 Pina, Fernando/C-8161-2011; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Pina, Fernando/0000-0001-8529-6848; 16

Preparation and photophysical characterisation of Zn-Allayered double hydroxides intercalated by anionic pyrene derivatives - Gago, Sandra; Costa, Telma; de Melo, J. Seixas; Goncalves, Isabel S.; Pillinger, Martyn (Journal of Materials Chemistry, 2008)
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Times Cited: 37 Seixas de Melo, Sergio/A-5388-2008; Gago, Sandra/H-5413-2012; Pillinger, Martyn/H-2639-2013; Goncalves, Isabel/H-3742-2013; Costa, Telma/C-9889-2010; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Seixas de Melo, Sergio/0000-0001-9708-5079; Gago, Sandra/0000-0003-0221-8508; Pillinger, Martyn/0000-0002-6243-7692; Goncalves, Isabel/0000-0002-2836-7715; Costa, Telma/0000-0002-5342-3635; 37

Probing the microenvironment of sol-gel entrapped cutinase: The role of added zeolite NaY - Vidinha, Pedro; Augusto, Vera; Nunes, Joao; Lima, Joao Carlos; Cabral, Joaquim M. S.; Barreiros, Susana (Journal of Biotechnology, 2008)
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Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-beta-D-xylanase 10B in complex with xylohexaose - Najmudin, Shabir; Pinheiro, Benedita A.; Romao, Maria J.; Prates, Jose A. M.; Fontes, Carlos M. G. A. (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2008)
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Times Cited: 2 Romao, Maria/A-4115-2013; Pinheiro, Benedita/D-2055-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; Prates, Jose/K-9934-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Pinheiro, Benedita/0000-0002-3045-077X; Prates, Jose/0000-0003-1032-5987; 8 2

Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 - Gavel, Olga Yu; Kladova, Anna V.; Bursakov, Sergey A.; Dias, Joao M.; Texeira, Susana; Shnyrov, Valery L.; Moura, Jose J. G.; Moura, Isabel; Romao, Maria J.; Trincao, Jose (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2008)
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Times Cited: 0 Romao, Maria/A-4115-2013; Moura, Isabel/D-6339-2013; Moura, Jose/D-6426-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Moura, Isabel/0000-0003-0971-4977; 7 0

A remote laboratory environment for blended learning - Coito, F.; Palma, L. B. (Proceedings of the 1st international conference on PErvasive Technologies Related to Assistive Environments, 2008)
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Sharpless asymmetric dihydroxylation of olefins in water-surfactant media with recycling of the catalytic system by membrane nanofiltration - Branco, Luis C.; Ferreira, Frederico Castelo; Santos, Jose L.; Crespo, Joao G.; Afonso, Carlos A. M. (Advanced Synthesis & Catalysis, 2008)
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Times Cited: 8 Branco, Luis/C-6426-2013; Crespo, Joao/D-8719-2013; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 8

Study of the effect of the phosphane bridging chain nature on the structural and photophysical properties of a series of gold(I) ethynylpyridine complexes - Ferrer, Montserrat; Gutierrez, Albert; Rodriguez, Laura; Rossell, Oriol; Lima, Joao C.; Font-Bardia, Merce; Solans, Xavier (European Journal of Inorganic Chemistry, 2008)
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Synthesis, characterization, and photophysical properties of 2-hydroxybenzaldehyde [(1E)-1-pyridin-2-ylethylidene]hydrazone and its rhenium(I) complexes - Barbazan, Paula; Carballo, Rosa; Covelo, Berta; Lodeiro, Carlos; Lima, Joao Carlos; Vazquez-Lopez, Ezequiel M. (European Journal of Inorganic Chemistry, 2008)
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Synthesis, spectroscopic, structural and complexation studies of a new tetra-naphthylmethylene pendant-armed macrocyclic ligand - Nunez, Cristina; Oliveira, Elisabete; Leticia Giestas; Valencia, Laura; Macias, Alejandro; Lima, Joao Carlos; Bastida, Rufina; Lodeiro, Carlos (Inorganica Chimica Acta, 2008)
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The use of microspectrofluorimetry for the characterization of lake pigments - Claro, Ana; Melo, Maria J.; Schafer, Stephan; de Melo, J. Sergio Seixas; Pina, Fernando; van den Berg, Klaas Jan; Burnstock, Aviva (Talanta, 2008)
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Times Cited: 21 Melo, Maria Joao/C-8594-2011; Pina, Fernando/C-8161-2011; Seixas de Melo, Sergio/A-5388-2008; Caparica, cqfb_staff/H-2611-2013; REQUIMTE, AL/H-9106-2013; Chaves, Pedro/K-1288-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, LAQV/N-9835-2013 Melo, Maria Joao/0000-0001-7393-6801; Pina, Fernando/0000-0001-8529-6848; Seixas de Melo, Sergio/0000-0001-9708-5079; 21

The Use of Remote Laboratory Activities within the Learning Process - Coito, F.; Gomes, L.; Costa, A.; Palma, L. (2008)
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{High-Performance Flexible Hybrid Field-Effect Transistors Based on Cellulose Fiber Paper} - Elvira Fortunato; Correia, Nuno; Barquinha, Pedro; Pereira, LuÍs; Goncalves, GonÇalo; Rodrigo Martins (IEEE Electron Device Letters, 2008)
In this letter, we report for the first time the use of a sheet of cellulose-fiber-based paper as the dielectric layer used in oxide-based semiconductor thin-film field-effect transis- tors (FETs). In this new approach, we are using the cellulose– fiber-based paper in an “interstrate” structure since the device is built on both sides of the cellulose sheet. Such hybrid FETs present excellent operating characteristics such as high channel saturation mobility (> 30 cm2/Vs), drain–source current on/off modulation ratio of approximately 104, near-zero threshold voltage, enhance- ment n-type operation, and subthreshold gate voltage swing of 0.8 V/decade. The cellulose-fiber-based paper FETs’ character- istics have been measured in air ambient conditions and present good stability, after two months of being processed. The obtained results outpace those of amorphous Si thin-film transistors (TFTs) and rival with the same oxide-based TFTs produced on either glass or crystalline silicon substrates. The compatibility of these devices with large-scale/large-area deposition techniques and low– cost substrates as well as their very low operating bias delin- eates this as a promising approach to attain high-performance disposable electronics like paper displays, smart labels, smart packaging, RFID, and point-of-care systems for self-analysis in bioapplications, among others.
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{Write-erase and read paper memory transistor} - Rodrigo Martins; Barquinha, Pedro; Pereira, Luís; Correia, Nuno; Gonçalves, Gonçalo; Ferreira, Isabel; Elvira Fortunato (Applied Physics Letters, 2008)
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Riesz potentials as centred derivatives - Ortigueira, M.D. (2nd Symposium on Fractional Derivatives and Their Applications, 2007)
Generalised fractional centred differences and derivatives are studied in this chapter. These generalise to real orders the existing ones valid for even and odd positive integer orders. For each one, suitable integral formulations are presented. The limit computation inside the integrals leads to generalisations of the Cauchy derivative. Their computations using a special path lead to the well known Riesz potentials. A study for coherence is done by applying the definitions to functions with Fourier transform. The existence of inverse Riesz potentials is also studied.
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Riesz potentials as centred derivatives - Ortigueira, M.D. (2nd Symposium on Fractional Derivatives and Their Applications. (J Sabatier, OP Agrawal, Machado, J.A.T., Eds.).:93–112.: Springer, 2007)
Generalised fractional centred differences and derivatives are studied in this chapter. These generalise to real orders the existing ones valid for even and odd positive integer orders. For each one, suitable integral formulations are presented. The limit computation inside the integrals leads to generalisations of the Cauchy derivative. Their computations using a special path lead to the well known Riesz potentials. A study for coherence is done by applying the definitions to functions with Fourier transform. The existence of inverse Riesz potentials is also studied.

Ultrasonic assisted protein enzymatic digestion for fast protein identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Sonoreactor versus ultrasonic probe - Rial-Otero, R.; Carreira, R. J.; Cordeiro, F. M.; Moro, A. J.; Santos, H. M.; Vale, G.; Moura, I.; Capelo, J. L. (Journal of Chromatography A, 2007)
Two different ultrasonic energy sources, the sonoreactor and the ultrasonic probe, are compared for enzymatic digestion of proteins for protein identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDl-TOF-MS) using the peptide mass fingerprint (PMF) procedure. Variables such as (i) trypsin/protein ratio; (ii) sonication time; (iii) ultrasound amplitude; and (iv) protein concentration are studied and compared. As a general rule, the trypsin/protein ratio and the minimum protein concentration successfully digested are similar with both ultrasonic energy sources. Results showed that the time needed to digest proteins was shorter with the ultrasonic probe, 60 s versus 120 s, for the same amplitude of sonication, 50%. However, lower standard deviations and cleaner MALDI-TOF-MS spectra were obtained with the sonoreactor. In addition, the sonoreactor device provided higher sample throughput (6 samples for the sonoreactor versus 1 sample for the ultrasonic probe) and easier sample handling for lower sample volumes (25 mu l). Finally, a comparison of both methodologies for the specific identification of the adenylylsulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the procedure. (c) 2007 Elsevier B.V. All rights reserved.
Times Cited: 21

Assessing Human And Technological Dimensions In Virtual Team’S Operational Competences - Sampaio, José; António Moniz (University Library of Munich, Germany, 2007)
Cognitive task automation may lead to over trust, complacency and loss of the necessary work environment situation awareness. This is a major constraint in complex work organizations teamwork, ending up into an operational gap, between system developments and its understanding and usability, by operators. This document presents a summary of the main results of author’s research on operational decision processes and occupational competences, applied to the air traffic control operational reality. Introducing a human/technological complementary approach to virtual team’s conceptualisation, the results show there is a dimension to be followed in human/machine integration, which stands beyond interface design, and calls for a deeper human comprehension of technological agent’s structure and functionalities, which will, ultimately, require the development of an operational cognitive framework, where work processes and technological behaviour are integrated in professional competences, as he two faces of the same coin.
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Keywords: automation; Decision process; occupational compete; situation awareness; teamwork; work organization

Highly sensitive nitrite biosensor based on the electrical wiring of nitrite reductase by ZnCr-AQS LDH - Chen, H.; Mousty, C.; Cosnier, S.; Silveira, C.; Moura, J. J. G.; Almeida, M. G. (Electrochemistry Communications, 2007)
A biosensor for amperometric determination of nitrite was developed using cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans immobilized and electrically connected on a glassy carbon electrode by entrapment into redox active [ZnCr-AQS] layered double hydroxide containing anthraquinone-2-sulfonate (AQS). The transduction step corresponded to the electro-enzymatic reduction of nitrite by immobilized AQS molecules at -0.6 V. The biosensor showed a fast response to nitrite (5 s) with a linear range between 0.015 and 2.35 mu M, a sensitivity of 1.8 A M-1 cm(-2) and a detection limit of 4 nM. The apparent Michaelis-Menten constant (K-M(app)) M was 7.5 mu M. (c) 2007 Elsevier B.V. All rights reserved.
Times Cited: 21

Improving sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass Spectrometry - Santos, H. M.; Rial-Otero, R.; Fernandes, L.; Vale, G.; Rivas, M. G.; Moura, I.; Capelo, J. L. (Journal of Proteome Research, 2007)
Three ultrasonic energy sources were studied to speed up the sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein reduction, alkylation, and enzymatic digestion steps were done in 15 min. Nine proteins, including zinc resistance-associated protein precursor from Desulfovibrio desulfuricans strain G20 and split-soret cytochrome c from D. desulfuricans ATCC27774 were successfully identified with the new protocol.
Times Cited: 10

{Assessing Human And Technological Dimensions In Virtual Team’S Operational Competences} - Sampaio, José; António Moniz (University Library of Munich, Germany, 2007)
Cognitive task automation may lead to over trust, complacency and loss of the necessary work environment situation awareness. This is a major constraint in complex work organizations teamwork, ending up into an operational gap, between system developments and its understanding and usability, by operators. This document presents a summary of the main results of author’s research on operational decision processes and occupational competences, applied to the air traffic control operational reality. Introducing a human/technological complementary approach to virtual team’s conceptualisation, the results show there is a dimension to be followed in human/machine integration, which stands beyond interface design, and calls for a deeper human comprehension of technological agent’s structure and functionalities, which will, ultimately, require the development of an operational cognitive framework, where work processes and technological behaviour are integrated in professional competences, as he two faces of the same coin.
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Keywords: automation; Decision process; occupational compete; situation awareness; teamwork; work organization

Residue-specific NH exchange rates studied by NMR diffusion experiments (vol 187, pg 97, 2007) - Brand, Torsten; Cabrita, Eurico J.; Morris, Gareth A.; Guenther, Robert; Hofmann, Hans-Joerg; Berger, Stefan (Journal of Magnetic Resonance, 2007)
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Editorial Note - António Moniz (Enterprise and Work Innovation Studies, 2007)
No abstract is available for this item.
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Into a new phase of the research on restructuring of work in the knowledge society: the Third WORKS General Assembly in Sofia (Bulgaria) - Moniz, António B.; Margarida R. Paulos (Enterprise and Work Innovation Studies, 2007)
The WORKS Project started two years ago (2005), involving the efforts of research institutes of 13 European countries with the main purpose of improving the understanding of the major changes in work in the knowledge-based society, taking account both of global forces and the regional diversity within Europe. This research meeting in Sofia (Bulgaria) aimed to present synthetically the massive amount of data collected in the case studies (occupational and organisational) and with the quantitative research during last year.
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Keywords: knowledge-based society; Work

Editorial Note - António Moniz (Enterprise and Work Innovation Studies, 2007)
No abstract is available for this item.
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{Into a new phase of the research on restructuring of work in the knowledge society: the Third WORKS General Assembly in Sofia (Bulgaria)} - Moniz, António B.; Margarida R. Paulos (Enterprise and Work Innovation Studies, 2007)
The WORKS Project started two years ago (2005), involving the efforts of research institutes of 13 European countries with the main purpose of improving the understanding of the major changes in work in the knowledge-based society, taking account both of global forces and the regional diversity within Europe. This research meeting in Sofia (Bulgaria) aimed to present synthetically the massive amount of data collected in the case studies (occupational and organisational) and with the quantitative research during last year.
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Keywords: knowledge-based society; Work

Conformations generated during turnover of the Azotobacter vinelandii nitrogenase MoFe protein and their relationship to physiological function - Fisher, Karl; Lowe, David J.; Tavares, Pedro; Pereira, Alice S.; Huynh, Boi Hanh; Edmondson, Dale; Newton, William E. (Journal Of Inorganic Biochemistry, 2007)
Various S = 3/2 EPR signals elicited from wild-type and variant Azotobacter vinelandii nitrogenase MoFe proteins appear to reflect different conformations assumed by the FeMo-cofactor with different protonation states. To determine whether these presumed changes in protonation and conformation reflect catalytic capacity, the responses (particularly to changes in electron flux) of the alpha H195Q, alpha H195N, and alpha Q191 K variant MoFe proteins (where His at position 195 in the alpha subunit is replaced by Gln/Asn or Gln at position alpha-191 by Lys), which have strikingly different substrate-reduction properties, were studied by stopped-flow or rapid-freeze techniques. Rapid-freeze EPR at low electron flux (at 3-fold molar excess of wild-type Fe protein) elicited two transient FeMo-cofactor-based EPR signals within 1 s of initiating turnover under N-2 with the alpha H195Q and alpha H195N variants, but not with the alpha Q191K variant. No EPR signals attributable to P cluster oxidation were observed for any of the variants under these conditions. Furthermore, during turnover at low electron flux with the wild-type, alpha H195Q or alpha H195N MoFe protein, the longer-time 430-nm absorbance increase, which likely reflects P cluster oxidation, was also not observed (by stopped-flow spectrophotometry); it did, however, occur for all three MoFe proteins under higher electron flux. No 430-nm absorbance increase occurred with the alpha Q191K variant, not even at higher electron flux. This putative lack of involvement of the P cluster in electron transfer at low electron flux was confirmed by rapid-freeze Fe-57 Mossbauer spectroscopy, which clearly showed FeMo-factor reduction without P cluster oxidation. Because the wild-type, alpha H195Q and alpha H195N MoFe proteins can bind N-2, but alpha Q195K cannot, these results suggest that P cluster oxidation occurs only under high electron flux as required for N-2 reduction. (C) 2007 Elsevier Inc. All rights reserved.
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EPR characterization of the molybdenum(V) forms of formate dehydrogenase from Desulfovibrio desulfuricans ATCC 27774 upon formate reduction - Rivas, M. G.; Gonzalez, P. J.; Brondino, C. D.; Moura, J. J.; Moura, I. (J Inorg Biochem, 2007)
The EPR characterization of the molybdenum(V) forms obtained on formate reduction of both as-prepared and inhibited formate dehydrogenase from Desulfovibrio desulfuricans ATCC 27774, an enzyme that catalyzes the oxidation of formate to CO(2), is reported. The Mo(V) EPR signal of the as-prepared formate-reduced enzyme is rhombic (g(max)=2.012, g(mid)=1.996, g(min)=1.985) and shows hyperfine coupling with two nuclear species with I=1/2. One of them gives an anisotropic splitting and is not solvent exchangeable (A(max)=11.7, A(mid)=A(min)=non-detectable, A-values in cm(-1)x10(-4)). The second species is exchangeable with solvent and produces a splitting at the three principal g-values (A(max)=7.7, A(mid)=10.0, A(min)=9.3). The hyperfine couplings of the non-solvent and solvent exchangeable nuclei are assigned to the hydrogen atoms of the beta-methylene carbon of a selenocysteine and to a Mo ligand whose nature, sulfydryl or hydroxyl, is still in debate. The Mo(V) species obtained in the presence of inhibitors (azide or cyanide) yields a nearly axial EPR signal showing only one detectable splitting given by nuclear species with I=1/2 (g(max)=2.092, g(mid)=2.000, g(min)=1.989, A(max)=non-detectable, A(mid)=A(min)=7.0), which is originated from the alpha-proton donated by the formate to a proximal ligand of the molybdenum. The possible structures of both paramagnetic molybdenum species (observed upon formate reduction in presence and absence of inhibitors) are discussed in comparison with the available structural information of this enzyme and the structural and EPR properties of the closely related formate dehydrogenase-H from Escherichia coli.
0162-0134 (Print)0162-0134 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Desulfovibrio desulfuricans/*enzymology; Electron Spin Resonance Spectroscopy/*methods; Formate Dehydrogenases/*chemistry/metabolism; Formates/*chemistry; Hydrogenase/*chemistry/metabolism; Kinetics; Models, Molecular; Molybdenum/*chemistry; Multienzyme Complexes/*chemistry/metabolism; Oxidation-Reduction

Main features of the labour policy in Portugal - António Moniz; Woll, Tobias (University Library of Munich, Germany, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment; gender; knowledge society; labour market; Policy; work organisation

{Main features of the labour policy in Portugal} - António Moniz; Woll, Tobias (University Library of Munich, Germany, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment; gender; knowledge society; labour market; Policy; work organisation

Biosensing nitrite using the system nitrite redutase/Nafion/methyl viologen--a voltammetric study - Almeida, M. G.; Silveira, C. M.; Moura, J. J. (Biosens Bioelectron, 2007)
This work describes the construction and voltammetric characterization of a nitrite biosensor based on a cytochrome c-type nitrite reductase (ccNiR) and the Nafion ionomeric matrix loaded with methyl viologen as redox mediator. Despite the potential electrostatic repulsions between the anionic substrate and the Nafion sulfonate groups, the resulting bioelectrode exhibited electrocatalytic activity toward nitrite. This phenomenon must be due to the nonuniformity of the enzyme/Nafion membrane, which allows the direct interaction between the substrate and numerous enzyme molecules. Nevertheless, the anionic nature of Nafion exerted a certain diffusion barrier to nitrite, as revealed by the unusually elevated limits of the linear dynamic range and k(m)(app). The irregularity of the composite membrane also contributed to slow down the rate of charge transfer throughout the Nafion polymer. The level of viologens incorporated within the Nafion membrane had a strong influence in the analytical parameters: as much mediator was present, lower was the sensitivity and wider was the linear range. For an optimized ratio enzyme/mediator the sensitivity was 445+/-8 mA M(-1)cm(-2), within the linear range 75-800 microM; the lowest detected nitrite concentration was 60 microM. The operational stability of the biosensor and the influence of some possible interferences were evaluated.
0956-5663 (Print)0956-5663 (Linking)Evaluation StudiesJournal ArticleResearch Support, Non-U.S. Gov't
Keywords: Biosensing Techniques/*methods; Electrochemistry/*methods; Enzymes, Immobilized/chemistry; Fluorocarbon Polymers/*chemistry; Microchemistry/*methods; Nitrite Reductases/*chemistry; Nitrites/*analysis/*chemistry; Paraquat/*chemistry; Reproducibility of Results; Sensitivity and Specificity

Gas chromatography mass spectrometry determination of acaricides from honey after a new fast ultrasonic-based solid phase micro-extraction sample treatment - Rial-Otero, R.; Gaspar, E. M.; Moura, I.; Capelo, J. L. (Talanta, 2007)
A method is reported for the determination of acaricides (amitraz, bromopropylate, coumaphos and fluvalinate) from honey by gas chromatography mass spectrometry after a new fast solid phase micro-extraction, SPME, procedure. Six different fibers were assessed for micro-extraction purpose studying the following variables: (i) SPME coating, (ii) extraction temperature, (iii) extraction time, (iv) desorption conditions and (v) agitation conditions. The new ultrasonic bath technology providing different sonication frequencies (35 and 130 kHz) and different working modes (Sweep, Standard and Degas) was studied and optimized for speeding up the acaricide micro-extraction. The best extraction results were achieved with the polyacrylate fiber. The extraction process was done in 30 min using the ultrasonic bath at 130 kHz in the Standard mode. Quality parameters of the proposed method show a good precision (<11%) and detection and quantitation limits lower than 6 and 15 ng/g, respectively, except for fluvalinate. Eleven Portuguese commercial honey samples were analyzed with the developed method in order to assess the performance of the method with real samples and to determine whether the concentration of acaricides in honey exceed their maximum residue levels (MRLs). Acaricide residues detected were lower than those established by the legislation. (c) 2006 Elsevier B.V. All rights reserved.
Times Cited: 15

Application of lactate amperometric sol-gel biosensor to sequential injection determination of L-lactate - Gomes, S. P.; Odlozilikova, M.; Almeida, M. G.; Araujo, A. N.; Couto, C. M.; Montenegro, M. C. (J Pharm Biomed Anal, 2007)
This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5x10(-5) to 5x10(-3)M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h(-1). The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.
Gomes, Sofia PiedadeOdlozilikova, MartinaAlmeida, M GabrielaAraujo, Alberto NCouto, Cristina M C MMontenegro, M Conceicao B S MResearch Support, Non-U.S. Gov'tEnglandJournal of pharmaceutical and biomedical analysisJ Pharm Biomed Anal. 2007 Mar 12;43(4):1376-81. Epub 2007 Jan 4.
Keywords: *Phase Transition; Biosensing Techniques/instrumentation/*methods; Electrochemistry; Enzymes, Immobilized/*analysis/chemistry; Gels; Kinetics; Lactic Acid/*analysis/chemistry; Pharmaceutical Preparations/analysis/chemistry

Correlating EPR and X-ray structural analysis of arsenite-inhibited forms of aldehyde oxidoreductase - Thapper, A.; Boer, D. R.; Brondino, C. D.; Moura, J. J.; Romao, M. J. (J Biol Inorg Chem, 2007)
Two arsenite-inhibited forms of each of the aldehyde oxidoreductases from Desulfovibrio gigas and Desulfovibrio desulfuricans have been studied by X-ray crystallography and electron paramagnetic resonance (EPR) spectroscopy. The molybdenum site of these enzymes shows a distorted square-pyramidal geometry in which two ligands, a hydroxyl/water molecule (the catalytic labile site) and a sulfido ligand, have been shown to be essential for catalysis. Arsenite addition to active as-prepared enzyme or to a reduced desulfo form yields two different species called A and B, respectively, which show different Mo(V) EPR signals. Both EPR signals show strong hyperfine and quadrupolar couplings with an arsenic nucleus, which suggests that arsenic interacts with molybdenum through an equatorial ligand. X-ray data of single crystals prepared from EPR-active samples show in both inhibited forms that the arsenic atom interacts with the molybdenum ion through an oxygen atom at the catalytic labile site and that the sulfido ligand is no longer present. EPR and X-ray data indicate that the main difference between both species is an equatorial ligand to molybdenum which was determined to be an oxo ligand in species A and a hydroxyl/water ligand in species B. The conclusion that the sulfido ligand is not essential to determine the EPR properties in both Mo-As complexes is achieved through EPR measurements on a substantial number of randomly oriented chemically reduced crystals immediately followed by X-ray studies on one of those crystals. EPR saturation studies show that the electron transfer pathway, which is essential for catalysis, is not modified upon inhibition.
0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Aldehyde Oxidoreductases/*antagonists & inhibitors/*chemistry; Arsenites/chemistry/*pharmacology; Bacterial Proteins/*antagonists & inhibitors/*chemistry; Crystallography, X-Ray; Desulfovibrio desulfuricans/*enzymology; Desulfovibrio gigas/*enzymology; Electron Spin Resonance Spectroscopy; Metals, Heavy; Molybdenum/chemistry; Protein Conformation

Futuros da indústria automóvel: Qual a sua importância para a definição do produto, modelos de produção e estratégias de mobilidade? [Futures of automobile industry: How important is it for - António Moniz (University Library of Munich, Germany, 2007)
Some programs and research networks are dealing with topics associated to the automtive sector and they observe one of the most significative sectores of modern economies. Because of that is so interesting to forecast some possible changes in an horizon of 10 to 20 years. But this exercse must not be centred only on technical aspects of automobile construction, or on the design, or even on the mobility systems. How one can face these problems, and how it can be so decisivelly important, is what we will try to answer in this paper that resumes some of the debates on the foresight exercises in Germany (Futur) and on the automotive sector in Portugal (WorTiS).
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Keywords: assembly line; automotive industry; design; forecasting; foresight; mobility; Portugal

Globalization processes of value chains in clothing industry in Portugal: implication in the working structures - António Brandão Moniz; Ana V. Silva; Woll, Tobias; José J. Sampaio (HAL, 2007)
Some of the phenomena where the “globalization” concept is applied include the internationalization of markets, globalization of culture, pol{\'ıtical hegemony of world by some states, or groups of states, the increasing power of supranational institutions, and the development of a global division of labour. A starting point to understand the global division of work is the study of how companies are re-structuring, once they are the key-actors in the decision on which work should be found and where. The “value chains” describe each step in the productive process of a final product or service. Separated units of value chains can be in the same company (in-house) or in different companies (outsourced). Similarly they can be in a same local, or in other location. Normalization of business processes, combined with digitalization of information and the development of telecommunication networks made possible the tele-mediated work. This paper presents results from the European WORKS project, where are studied Portuguese cases of firms that integrate globalized value chain, and are analized the implications on work organization models and the (new) professional structures.
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Keywords: clothing industry; Europe; globalisation; Portugal; textile industry; value chains

Gold nanoparticle based systems in genetics - Gaspar, Jorge Francisco; Baptista, Pedro Viana; José Rueff (Current Pharmacogenomics, 2007)
Advances in nanoscience are having a significant impact on many scientific fields, boosting the development of a variety of important technologies. The impact of these new technologies is particularly large in biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecular detection. The physicochemical malleability and high surface areas of nanoparticle surfaces make them ideal candidates for developing biomarker platforms. Given the variety of strategies afforded through nanoparticle technologies, a significant goal is to tailor nanoparticle surfaces to selectively bind a subset of biomarkers, either for direct detection and characterization or to sequester the target molecules for later study using other available techniques. To date, applications of nanoparticles have largely focused on DNA- or protein-functionalized gold nanoparticles used as the target-specific probes. These unique biophysical properties displayed by gold nanoparticles have huge advantages over conventional detection methods (e.g., molecular fluorophores, microarray technologies). These gold-nanoparticle based systems can then be used for the detection of specific sequences of DNA (pathogen detection, characterization of mutation and/or SNPs) or RNA (without previous retro-transcription and amplification.
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Keywords: Cancer; Genotyping; Gold nanoparticles; Mutation detection; Nanosensors; nanotechnology; Pharmacogenetics

Qualificação e Competência Profissional Num Sistema Complexo De Trabalho (Qualification and professional competence in a complex working system) - Sampaio, José; António Brandão Moniz (HAL, 2007)
Nesta comunicação em que se discutem, numa perspectiva de complementaridade, os conceitos de qualificação e de competência profissional, dá-se conta da metodologia adoptada para a identificação e validação de um conjunto de competências profissionais num sistema complexo de trabalho. O estudo incidiu sobre os Serviços de Controlo de Tráfego Aéreo da Região de Informação de Voo de Lisboa e permitiu concluir pela existência de uma dimensão integrativa das componentes trabalho e tecnologia, enquanto elementos estruturantes dos mapas de competências profissionais, indispensáveis à integração sistémica dos diferentes agentes (humanos e tecnológicos) no processo produtivo.
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Keywords: air traffic control; complex working systems; Professional competences

{Futuros da indústria automóvel: Qual a sua importância para a definição do produto, modelos de produção e estratégias de mobilidade? [Futures of automobile industry: How important is it for the def} - António Moniz (University Library of Munich, Germany, 2007)
Some programs and research networks are dealing with topics associated to the automtive sector and they observe one of the most significative sectores of modern economies. Because of that is so interesting to forecast some possible changes in an horizon of 10 to 20 years. But this exercse must not be centred only on technical aspects of automobile construction, or on the design, or even on the mobility systems. How one can face these problems, and how it can be so decisivelly important, is what we will try to answer in this paper that resumes some of the debates on the foresight exercises in Germany (Futur) and on the automotive sector in Portugal (WorTiS).
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Keywords: assembly line; automotive industry; design; forecasting; foresight; mobility; Portugal

{Globalization processes of value chains in clothing industry in Portugal: implication in the working structures} - António Brandão Moniz; Ana V. Silva; Woll, Tobias; José J. Sampaio (HAL, 2007)
Some of the phenomena where the “globalization” concept is applied include the internationalization of markets, globalization of culture, polítical hegemony of world by some states, or groups of states, the increasing power of supranational institutions, and the development of a global division of labour. A starting point to understand the global division of work is the study of how companies are re-structuring, once they are the key-actors in the decision on which work should be found and where. The “value chains” describe each step in the productive process of a final product or service. Separated units of value chains can be in the same company (in-house) or in different companies (outsourced). Similarly they can be in a same local, or in other location. Normalization of business processes, combined with digitalization of information and the development of telecommunication networks made possible the tele-mediated work. This paper presents results from the European WORKS project, where are studied Portuguese cases of firms that integrate globalized value chain, and are analized the implications on work organization models and the (new) professional structures.
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Keywords: clothing industry; Europe; globalisation; Portugal; textile industry; value chains

{Qualificação e Competência Profissional Num Sistema Complexo De Trabalho (Qualification and professional competence in a complex working system)} - Sampaio, José; António Brandão Moniz (HAL, 2007)
Nesta comunicação em que se discutem, numa perspectiva de complementaridade, os conceitos de qualificação e de competência profissional, dá-se conta da metodologia adoptada para a identificação e validação de um conjunto de competências profissionais num sistema complexo de trabalho. O estudo incidiu sobre os Serviços de Controlo de Tráfego Aéreo da Região de Informação de Voo de Lisboa e permitiu concluir pela existência de uma dimensão integrativa das componentes trabalho e tecnologia, enquanto elementos estruturantes dos mapas de competências profissionais, indispensáveis à integração sistémica dos diferentes agentes (humanos e tecnológicos) no processo produtivo.
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Keywords: air traffic control; complex working systems; Professional competences

Neurotoxicity mechanisms of thioether ecstasy metabolites - Capela, JP; Macedo, C.; Branco, P. S.; Ferreira, LM; A. M. Lobo; Fernandes, E; Remiao, F; Bastos, ML; Dirnagl, U.; Meisel, A; F. Carvalho (NEUROSCIENCE, 2007)
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New findings for in-gel digestion accelerated by high-intensity focused ultrasound for protein identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry - Carreira, R. J.; Cordeiro, F. M.; Moro, A. J.; Rivas, M. G.; Rial-Otero, R.; Gaspar, E. M.; Moura, I.; Capelo, J. L. (Journal of Chromatography A, 2007)
New findings in sample treatment based on high-intensity focused ultrasound (HIFU) for protein digestion after polyacrylamide gel electrophoresis separation are presented. The following variables were studied: (i) sample volume; (ii) sonotrode diameter; (iii) previous protein denaturation; (iv) cooling; (v) enzyme concentration; and (vi) protein concentration. Results showed that positive protein identification could be done after protein separation by gel electrophoresis through peptide mass fingerprint (PMF) in a volume as low as 25 mu L. The time needed was less than 2 min and no cooling was necessary. The importance of the sonotrode diameter was negligible. On the other hand, protein denaturation before sonication was a trade-off for the success of procedure here described. The protein coverage was raised from 5 to 30%, and the number of peptides matching the proteins was also increased in a percentage ranging 10-100% when the classical overnight treatment is compared with the proposed HIFU procedure. The minimum amount of protein that can be identified using the HIFU sample treatment by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was 0.06 mu g. The lower concentration of trypsin successfully used to obtain an adequate protein digestion was 3.6 mu g/mL. (c) 2006 Elsevier B.V. All rights reserved.
Times Cited: 15

Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purification, crystallization and preliminary X-ray analysis - Coelho, C.; Gonzalez, P. J.; Trincao, J.; Carvalho, A. L.; Najmudin, S.; Hettman, T.; Dieckman, S.; Moura, J. J.; Moura, I.; Romao, M. J. (Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007)
The periplasmic nitrate reductase from Cupriavidus necator (also known as Ralstonia eutropha) is a heterodimer that is able to reduce nitrate to nitrite. It comprises a 91 kDa catalytic subunit (NapA) and a 17 kDa subunit (NapB) that is involved in electron transfer. The larger subunit contains a molybdenum active site with a bis-molybdopterin guanine dinucleotide cofactor as well as one [4Fe-4S] cluster, while the small subunit is a di-haem c-type cytochrome. Crystals of the oxidized form of this enzyme were obtained using polyethylene glycol 3350 as precipitant. A single crystal grown at the High Throughput Crystallization Laboratory of the EMBL in Grenoble diffracted to beyond 1.5 A at the ESRF (ID14-1), which is the highest resolution reported to date for a nitrate reductase. The unit-cell parameters are a = 142.2, b = 82.4, c = 96.8 A, beta = 100.7 degrees, space group C2, and one heterodimer is present per asymmetric unit.
1744-3091 (Electronic)1744-3091 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Bacterial Proteins/chemistry/*isolation & purification; Crystallization/methods; Crystallography, X-Ray; Cupriavidus necator/*isolation & purification; Dimerization; Nitrate Reductase/chemistry/*isolation & purification; Protein Subunits/chemistry/isolation & purification

Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer - de Sousa, P. M.; Pauleta, S. R.; Goncalves, M. L.; Pettigrew, G. W.; Moura, I.; Dos Santos, M. M.; Moura, J. J. (J Biol Inorg Chem, 2007)
This work reports the direct electrochemistry of Paracoccus pantotrophus pseudoazurin and the mediated catalysis of cytochrome c peroxidase from the same organism. The voltammetric behaviour was examined at a gold membrane electrode, and the studies were performed in the presence of calcium to enable the peroxidase activation. A formal reduction potential, E (0)', of 230 +/- 5 mV was determined for pseudoazurin at pH 7.0. Its voltammetric signal presented a pH dependence, defined by pK values of 6.5 and 10.5 in the oxidised state and 7.2 in the reduced state, and was constant up to 1 M NaCl. This small copper protein was shown to be competent as an electron donor to cytochrome c peroxidase and the kinetics of intermolecular electron transfer was analysed. A second-order rate constant of 1.4 +/- 0.2 x 10(5) M(-1) s(-1) was determined at 0 M NaCl. This parameter has a maximum at 0.3 M NaCl and is pH-independent between pH 5 and 9.
0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Azurin/*metabolism; Catalysis; Cytochrome-c Peroxidase/*metabolism; Electrochemistry; Electrodes; Electrolytes; Electron Transport/*physiology; Hydrogen Peroxide/chemistry; Hydrogen-Ion Concentration; Indicators and Reagents; Kinetics; Paracoccus pantotrophus/*enzymology

Preparation of ingredients containing an ACE-inhibitory peptide by tryptic hydrolysis of whey protein concentrates - Ferreira, I M P L V O; Pinho, O; Mota, M. V.; Tavares, P; Pereira, A.; Goncalves, M. P.; Torres, D.; Rocha, C.; Teixeira, J. A. (INTERNATIONAL DAIRY JOURNAL, 2007)
This study describes the characterisation of whey protein hydrolysates obtained from tryptic hydrolysis to assess their application as ingredients with angiotensin-converting-enzyme (ACE) inhibitory action. The levels of a-lactalbumin (alpha-la) and P-lactoglobulin (beta-lg) remaining after hydrolysis were quantified. Peptides were separated by RP-HPLC, and Ala-Leu-Pro-Met-His-Ile-Arg (ALPMHIR), the most potent beta-lg-derived ACE-inhibitory peptide was monitored. A correlation curve was established for the production of this peptide as a function of hydrolysis time. Heat-induced gelation of hydrolysates was studied by small-deformation rheology. The gelation times and the strength of the final gels were highly dependent on the degree of hydrolysis. Smaller peptides liberated by hydrolysis contributed to the inability of whey protein hydrolysates to gel. (c) 2006 Elsevier Ltd. All rights reserved.
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{Wandel der Arbeit: Die Krise der Arbeitsgesellschaft [Change on Work: the crisis of the labour economy]} - Krings, Bettina (University Library of Munich, Germany, 2007)
In 1982, at the 21st German Congress of Sociology the discussion was around a new topic: the crisis of the labour economy. Since then the conditions changed and the informatisation and technology development of work environments took place. Here are presented some of the new trends in terms of analisis of changes in the work environment. Der im Jahre 1982 durchgeführte 21. Deutschen Soziologentag in Bamberg mit dem Titel „Krise der Arbeitsgesellschaft?“ wurde sicherlich bewusst mit einem Fragezeichen versehen. Wenn im Rahmen der „Verhandlungen“ in Bamberg noch die Möglichkeit ausgeschlossen wurde, wissensbasierte Tätigkeitsfelder zu „normieren“, so wurden über die beiden organisatorischen Instrumente der Selbstorganisation und der Flexibilisierung höchst effektive Rahmenbedingungen geschaffen, um die Nutzung der Arbeitskraft zu kontrollieren und zu steigern. Aus unterschiedlichen Blickwinkeln und Arbeitskontexten beschäftigen sich alle Beiträge des vorliegenden Schwerpunktes mit dem Wandel der Arbeit.
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Keywords: controversies; labour economy; technology

Palladium(II) mediated aziridination of olefins with bromamine-T as the nitrogen source: scope and mechanism - Antunes, Alexandra M. M.; Bonifacio, Vasco D. B.; Nascimento, Susana C. C.; Lobo, Ana M; Branco, Paula S.; Prabhakar, Sundaresan (TETRAHEDRON, 2007)
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Residue-specific NH exchange rates studied by NMR diffusion experiments - Brand, Torsten; Cabrita, Eurico J.; Morris, Gareth A.; Guenther, Robert; Hofmann, Hans-Jorg; Berger, Stefan (Journal of Magnetic Resonance, 2007)
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Crystal structure of the 16 heme cytochrome from Desulfovibrio gigas: A glycosylated protein in a sulphate-reducing bacterium - Santos-Silva, Teresa; Dias, Joao Miguel; Dolla, Alain; Durand, Marie-Claire; Goncalves, Luisa L.; Lampreia, Jorge; Moura, Isabel; Romao, Maria Joao (Journal of Molecular Biology, 2007)
Sulphate-reducing bacteria have a wide variety of periplasmic cytochromes involved in electron transfer from the periplasm to the cytoplasm. HmcA is a high molecular mass cytochrome of 550 amino acid residues that harbours 16 c-type heme groups. We report the crystal structure of HmcA isolated from the periplasm of Desulfovibrio gigas. Crystals were grown. using polyethylene glycol 8K and zinc acetate, and diffracted beyond 2.1 angstrom resolution. A multiple-wavelength anomalous dispersion experiment at the iron absorption edge enabled us to obtain good-quality phases for structure solution and model building. DgHmcA has a V-shape architecture, already observed in HmcA isolated from Desulfovibrio vulgaris Hildenborough. The presence of an oligosaccharide molecule covalently bound to an Asn residue was observed in the electron density maps of DgHmcA and confirmed by mass spectrometry. Three modified monosaccharides appear at the highly hydrophobic vertex, possibly acting as an anchor of the protein to the cytoplasmic membrane. (c) 2007 Elsevier Ltd. All rights reserved.
Times Cited: 7

NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster - Pauleta, S. R.; Duarte, A. G.; Carepo, M. S.; Pereira, A. S.; Tavares, P; Moura, I.; Moura, J. J. (Biomol NMR Assign, 2007)
We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo-Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.
1874-270X (Electronic)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Apoproteins/chemistry/genetics; Bacterial Proteins/*chemistry/genetics; Binding Sites; Carbon Isotopes; Copper/chemistry; Desulfovibrio gigas/*chemistry/genetics; Molecular Structure; Molybdenum/chemistry; Nuclear Magnetic Resonance, Biomolecular; Recombinant Proteins/chemistry/genetics

NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster - Pauleta, Sofia R.; Duarte, Americo G.; Carepo, Marta S.; Pereira, Alice S.; Tavares, Pedro; Moura, Isabel; Moura, Jose J. G. (Biomolecular Nmr Assignments, 2007)
We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo-Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using C-13-detection experiments.
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Superoxide reductases - Pereira, Alice S.; Tavares, Pedro; Folgosa, Filipe; Almeida, Rui M.; Moura, Isabel; Moura, Jose J. G. (European Journal of Inorganic Chemistry, 2007)
Reactive oxygen species (ROS), when in excess, are among the most deleterious species an organism can deal with. The physiological effects of ROS include amino acid chain cleavage, DNA degradation and lipid oxidation, among others. They can be formed in the cytoplasm in a variety of ways, including autooxidation reactions (FMN- and FAD-containing enzymes) and Fenton reactions as a result of the cytoplasmatic pool of iron ions. The superoxide anion (021, despite its short half-life in solution, is particularly pernicious as it can form other reactive ROS (such as the strong oxidant peroxynitrite) or oxidize and/or reduce cellular components. For strict anaerobic or microaerophilic bacteria it is of particular importance to be able to dispose of ROS in a controlled manner, especially if these organisms are temporarily exposed to air. This review aims to describe the structural characteristics of superoxide reductases (SORs) and mechanistic aspects of biological superoxide anion reduction. SORs can be considered the main class of enzymes behind the oxygen detoxification pathway of anaerobic and microaerophilic bacteria. The geometry of the active site (three classes have been described), the possible electron donors in vivo and the current hypothesis for the catalytic mechanism will be discussed. Some phylogenetic considerations are presented, regarding the primary structure of SORs currently available in genome databases. ((c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007).
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A needle in a haystack: the active site of the membrane-bound complex cytochrome c nitrite reductase - Almeida, M. G.; Silveira, C. M.; Guigliarelli, B.; Bertrand, P.; Moura, J. J.; Moura, I.; Leger, C. (FEBS Lett, 2007)
Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the six-electron reduction of nitrite to ammonium. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes. The number of closely-spaced hemes in this enzyme and the magnetic interactions between them make it very difficult to study the active site by using traditional spectroscopic approaches such as EPR or UV-Vis. Here, we use both catalytic and non-catalytic protein film voltammetry to simply and unambiguously determine the reduction potential of the catalytic heme over a wide range of pH and we demonstrate that proton transfer is coupled to electron transfer at the active site.
0014-5793 (Print)0014-5793 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: Bacterial Proteins/*chemistry/isolation & purification; Binding Sites; Catalysis; Cytochromes a1/*chemistry/isolation & purification; Cytochromes c1/*chemistry/isolation & purification; Desulfovibrio desulfuricans/*enzymology; Heme/*chemistry; Nitrate Reductases/*chemistry/isolation & purification; Potentiometry; Protons

Amorphous/nanocrystalline silicon biosensor for the specific identification of unamplified nucleic acid sequences using gold nanoparticle probes - Martins, Rodrigo Ferrão de Paiva; Pedro Baptista; Leandro Raniero; Gonçalo Doria; Silva, L. B.; Ricardo Franco; Fortunato, Elvira Maria Correia (Applied Physics Letters, 2007)
Amorphous/nanocrystalline silicon pi'ii'n devices fabricated on micromachined glass substrates are integrated with oligonucleotide-derivatized gold nanoparticles for a colorimetric detection method. The method enables the specific detection and quantification of unamplified nucleic acid sequences (DNA and RNA) without the need to functionalize the glass surface, allowing for resolution of single nucleotide differences between DNA and RNA sequences-single nucleotide polymorphism and mutation detection. The detector's substrate is glass and the sample is directly applied on the back side of the biosensor, ensuring a direct optical coupling of the assays with a concomitant maximum photon capture and the possibility to reuse the sensor. (c) 2007 American Institute of Physics.
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Keywords: Bioassay; Biosensors; Colorimetry; DNA sequences; Gold; mutagenesis; Nanostructured materials; Optical properties; Photons

Imaging gold nanoparticles for DNA sequence recognition in biomedical applications - Baptista, Pedro Miguel Ribeiro Viana; Ricardo Franco (Ieee Transactions On Nanobioscience, 2007)
The hybridization of single-stranded oligonucleotide-derivatized gold nanoparticles (An nanoprobes) with double stranded complementary DNA was directly observed by atomic force microscopy (AFM). This specific interaction is the basis for an An nanoprobe-based homogeneous assay for specific DNA sequence detection, based on salt-induced particle aggregation that is prevented when a complementary target is present. For long DNA targets (linearized plasmid DNA) complicated hybridized target DNA-Au-nanoprobes structures were formed, that were interpreted as the basis for stability of the An nanoprobes against salt-induced aggregation. For shorter DNA targets (PCR amplified fragments) hybridization with the An nanoprobes occurred, in the majority of cases, in the expected location of the DNA target fragment containing the specific sequence. The formation of the observed DNA hybridized structures provides evidence at the molecular level for specific hybridization to the target sequence as the method of binding of the An nanoprobes.
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Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection - Baptista, Pedro Miguel Ribeiro Viana; Ricardo Franco (Iet Nanobiotechnology, 2007)
Advances in nanosciences are having a significant impact in many areas of research. The impact of new nanotechnologies has been particularly large in biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecules detection. To date, applications of nanoparticles have largely focused on DNA-functionalised gold nanoparticles used as the target-specific probes. These gold nanoparticle-based systems can be used for the detection of specific sequences of DNA (pathogen detection, characterisation of mutation and/or single nucleotide polymorphisms) or RNA (without prior retro-transcription and amplification). Here a rapid and inexpensive nanoparticle-based method for single-base mismatch detection (single nucleotide polymorphism/mutation) in DNA samples is reported. Gold nanoparticles derivatised with thiol modified oligonucleotides complementary to DNA targets - Au-nanoprobes - are used to distinguish fully complementary from mismatched sequences, with a single-base mismatch. The authors have successfully applied this strategy to detect common mutations within the beta-globin gene.
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Novel optoelectronic platform using an amorphous/nanocrystalline silicon biosensor for the specific identification of unamplified nucleic acid sequences based on gold nanoparticle probes - Silva, Leonardo Bione; Pedro Baptista; Leandro Raniero; Gonçalo Dória; Ricardo Franco; Martins, Rodrigo Ferrão de Paiva; Fortunato, Elvira Maria Correia (Solid-State Sensors, Actuators and Microsystems Conference, 2007, 2007)
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TRANSDUCERS 2007. International ; Conference date: 01-01-2007

Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration - Soares, S. S.; Martins, H.; Duarte, R. O.; Moura, J. J.; Coucelo, J.; Gutierrez-Merino, C.; Aureliano, M. (J Inorg Biochem, 2007)
The contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1mM total vanadium) in Sparus aurata. Although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. After 1, 6 and 12h upon decavanadate administration the increase of vanadium in blood plasma, red blood cells and in cardiac mitochondria and cytosol is not affected in comparison to the administration of a metavanadate solution containing labile oxovanadates. Cardiac tissue lipid peroxidation increases up to 20%, 1, 6 and 12h after metavanadate administration, whilst for decavanadate no effects were observed except 1h after treatment (+20%). Metavanadate administration clearly differs from decavanadate by enhancing, 12h after exposure, mitochondrial superoxide dismutase (SOD) activity (+115%) and not affecting catalase (CAT) activity whereas decavanadate increases SOD activity by 20% and decreases (-55%) mitochondrial CAT activity. At early times of exposure, 1 and 6h, the only effect observed upon decavanadate administration was the increase by 20% of SOD activity. In conclusion, decavanadate has a different response pattern of lipid peroxidation and oxidative stress markers, in spite of the same vanadium distribution in cardiac cells observed after decavanadate and metavanadate administration. It is suggested that once formed decameric vanadate species has a different reactivity than vanadate, thus, pointing out that the differential contribution of vanadium oligomers should be taken into account to rationalize in vivo vanadate toxicity.
0162-0134 (Print)0162-0134 (Linking)Journal ArticleResearch Support, Non-U.S. Gov't
Keywords: *Biological Markers; *Lipid Peroxidation; *Oxidative Stress; Animals; Catalase/metabolism; Magnetic Resonance Spectroscopy; Sea Bream; Subcellular Fractions/metabolism; Superoxide Dismutase/metabolism; Vanadates/administration & dosage/*pharmacokinetics

Molecular interactions and CO2-philicity in supercritical CO2. A high-pressure NMR and molecular modeling study of a perfluorinated polymer in scCO(2) - Temtem, Marcio; Casimiro, Teresa; Santos, A. Gil; Macedo, Anjos L.; Cabrita, Eurico J.; Aguiar-Ricardo, Ana (Journal of Physical Chemistry B, 2007)
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Chromatographic-based methods for pesticide determination in honey: An overview - Rial-Otero, R.; Gaspar, E. M.; Moura, I.; Capelo, J. L. (Talanta, 2007)
Nowadays the control of pesticides in honey is an issue of primary health importance as consequence of the increasing content of these chemicals in the aforementioned matrix. This poisoning has led to the worldwide increasing loss of bees since 1995. From Europe to Canada, scientist, beekeepers and chemical companies disagree about the reasons that have led to colony losses higher than 50% in some areas. This problem has become a public health issue due to the high honey worldwide consumption. The presence of pesticides in honey has been directly related to bees' mortality by some researchers through pesticide presence in (1) pollen, (2) honeycomb walls, (3) own bees and (4) honey. In this work we describe the actual state-of-the-art for pesticides determination in honey along with a review in this subject focused on sample treatments and instrumentation. Finally, future trends are also commented. (c) 2006 Elsevier B.V. All rights reserved.
Times Cited: 36

Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins - Rial-Otero, R.; Carreira, R. J.; Cordeiro, F. M.; Moro, A. J.; Fernandes, L.; Moura, I.; Capelo, J. L. (Journal of Proteome Research, 2007)
Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylylsulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the methodology. The new sample treatment is of easy implementation, saves time and money, and can be adapted to online procedures and robotic platforms.
Times Cited: 20

Synthesis and cyclic voltammetry studies of 3,4-methylenedioxymethamphetamine (MDMA) human metabolites - Macedo, Carla; Branco, Paula Serio; Ferreira, Luisa Maria; Lobo, Ana Maria; Capela, Joao Paulo; Fernandes, Eduarda; de Lourdes Bastos, Maria; Carvalho, Felix (JOURNAL OF HEALTH SCIENCE, 2007)
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Synthesis of gamma-amino acid analogues from natural alpha-amino acids by a radical pathway - Corvo, Mc; Pereira, M. M. A. (AMINO ACIDS, 2007)
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A new lupene triterpenetriol and anticholinesterase activity of Salvia sclareoides - Rauter, Amelia P.; Branco, Isabel; Lopes, Rui G.; Justino, Jorge; Silva, Filipa V. M.; Noronha, Joao P.; Cabrita, Eurico J.; Brouard, Ignacio; Bermejo, Jaime (Fitoterapia, 2007)
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Main features of the labour policy in Portugal - Moniz, António B.; Woll, Tobias (Universidade Nova de Lisboa, IET-Research on Enterprise and Work Innovation, Faculty of Science and Technology, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment policy; gender; knowledge society; labour market; work organisation

Main features of the labour policy in Portugal - António Brandão Moniz; Woll, Tobias (HAL, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment policy; gender; knowledge society; labour market; work organisation

{How restructuring is changing occupations? Case study evidence from knowledge-intensive, manufacturing and service occupations} - Valenduc, Gérard; Vendramin, Patricia; Krings, Bettina; Nierling, Linda (ZBW - German National Library of Economics, 2007)
This report is the final deliverable (D11.1) of the workpackage on occupational case studies of the WORKS project (WP11 – Qualitative research – Case studies on changes in work - Impacts on the individual and the household). In this workpackage, 30 occupational case studies were achieved in 14 countries, between June 2006 and May 2007; in total 246 in-depth individual interviews were carried out, according to common interview guidelines elaborated in May 2006, at the end of the workpackage on qualitative methods (WP6). These occupational case studies are closely related to the organisational case studies that were carried out in a selected number of business functions, during the same time span. In the WORKS project, business functions are at the core of qualitative empirical research, as they provide a relevant framework for analysis of value chain restructuring and changes in work. In order to study changes in work at the individual level, this report focuses on individual workers within occupational groups linked to key business functions. This link is justified in Chapter 2. Six occupational groups are considered in the report: designers in the clothing industry; researchers in information and communication technology; IT professionals in software services; production workers in food or clothing; logistics workers in food or clothing; front office employees in customer relationships in public services. In each occupational group, three to seven case studies were conducted an reported in different countries, covering a variety of socio-economic and institutional contexts. Each case study relies on seven to nine in-depth individual interviews, including a biographical dimension. In the first part of this report (Chapter 2), the research design and methodology are explained and justified. The key research questions are developed, as well as the concrete methodological choices and the practical organisation of the case studies. The second part of the report (Chapter
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Keywords: business function; clothing ind; occupations; qualitative research; restructuring; value chain; Work

{Main features of the labour policy in Portugal} - Moniz, António B.; Woll, Tobias (Universidade Nova de Lisboa, IET/CICS.NOVA-Interdisciplinary Centre on Social Sciences, Faculty of Science and Technology, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment policy; gender; knowledge society; labour market; work organisation

{Main features of the labour policy in Portugal} - António Brandão Moniz; Woll, Tobias (HAL, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment policy; gender; knowledge society; labour market; work organisation

{Main features of the labour policy in Portugal} - António Brandão Moniz; Woll, Tobias (HAL, 2007)
In this working paper is presented information on the Portuguese labour market developed with the support of the European project WORKS-“Work organisation and restructuring in the knowledge society”. Is still a on the process article and thus commentaries are welcome. The structure is based on the following topics: a) The employment policy (Time regimes - time use, flexibility, part-time work, work-life balance -, and the work contracts regimes – wages, contract types, diversity); b) Education and training (skilling outcomes, rules on retraining and further training, employability schemes, transferability of skills); c) Equal opportunities (relevance of equal opportunity regulation for restructuring outcomes, the role of gender and age regulation); d) Restructuring effects (policy on transfer of personnel, policy on redundancies, and participation or voice in restructuring).
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Keywords: Education; employment policy; gender; knowledge society; labour market; work organisation

NADH oxidase activity of rat and human liver xanthine oxidoreductase: potential role in superoxide production - Maia, L.; Duarte, R. O.; Ponces-Freire, A.; Moura, J. J.; Mira, L. (J Biol Inorg Chem, 2007)
To characterise the NADH oxidase activity of both xanthine dehydrogenase (XD) and xanthine oxidase (XO) forms of rat liver xanthine oxidoreductase (XOR) and to evaluate the potential role of this mammalian enzyme as an O2*- source, kinetics and electron paramagnetic resonance (EPR) spectroscopic studies were performed. A steady-state kinetics study of XD showed that it catalyses NADH oxidation, leading to the formation of one O2*- molecule and half a H(2)O(2) molecule per NADH molecule, at rates 3 times those observed for XO (29.2 +/- 1.6 and 9.38 +/- 0.31 min(-1), respectively). EPR spectra of NADH-reduced XD and XO were qualitatively similar, but they were quantitatively quite different. While NADH efficiently reduced XD, only a great excess of NADH reduced XO. In agreement with reductive titration data, the XD specificity constant for NADH (8.73 +/- 1.36 microM(-1) min(-1)) was found to be higher than that of the XO specificity constant (1.07 +/- 0.09 microM(-1) min(-1)). It was confirmed that, for the reducing substrate xanthine, rat liver XD is also a better O2*- source than XO. These data show that the dehydrogenase form of liver XOR is, thus, intrinsically more efficient at generating O2*- than the oxidase form, independently of the reducing substrate. Most importantly, for comparative purposes, human liver XO activity towards NADH oxidation was also studied, and the kinetics parameters obtained were found to be very similar to those of the XO form of rat liver XOR, foreseeing potential applications of rat liver XOR as a model of the human liver enzyme.
0949-8257 (Print)0949-8257 (Linking)Journal Article
Keywords: Animals; Electron Spin Resonance Spectroscopy; Humans; Kinetics; Liver/enzymology; Multienzyme Complexes/*metabolism; NAD/metabolism; NADH, NADPH Oxidoreductases/*metabolism; Rats; Superoxides/*metabolism; Xanthine Oxidase/*metabolism

Oxidation process of adrenaline in freshly isolated rat cardiomyocytes: Formation of adrenochrome, quinoproteins, and GSH adduct - Costa, Vera Marisa; Silva, Renata; Ferreira, Luisa Maria; Branco, Paula Serio; Carvalho, Felix; Bastos, Maria Lourdes; Carvalho, Rui Albuquerque; Carvalho, Marcia; Remiao, Fernando (CHEMICAL RESEARCH IN TOXICOLOGY, 2007)
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Spectroscopic, computational, and kinetic studies of the mu4-sulfide-bridged tetranuclear CuZ cluster in N2O reductase: pH effect on the edge ligand and its contribution to reactivity - Ghosh, S.; Gorelsky, S. I.; George, S. D.; Chan, J. M.; Cabrito, I.; Dooley, D. M.; Moura, J. J.; Moura, I.; Solomon, E. I. (J Am Chem Soc, 2007)
A combination of spectroscopy and density functional theory (DFT) calculations has been used to evaluate the pH effect at the CuZ site in Pseudomonas nautica (Pn) nitrous oxide reductase (N2OR) and Achromobacter cycloclastes (Ac) N2OR and its relevance to catalysis. Absorption, magnetic circular dichroism, and electron paramagnetic resonance with sulfur K-edge X-ray absorption spectra of the enzymes at high and low pH show minor changes. However, resonance Raman (rR) spectroscopy of PnN2OR at high pH shows that the 415 cm-1 Cu-S vibration (observed at low pH) shifts to higher frequency, loses intensity, and obtains a 9 cm-1 18O shift, implying significant Cu-O character, demonstrating the presence of a OH- ligand at the CuICuIV edge. From DFT calculations, protonation of either the OH- to H2O or the mu4-S2- to mu4-SH- would produce large spectral changes which are not observed. Alternatively, DFT calculations including a lysine residue at an H-bonding distance from the CuICuIV edge ligand show that the position of the OH- ligand depends on the protonation state of the lysine. This would change the coupling of the Cu-(OH) stretch with the Cu-S stretch, as observed in the rR spectrum. Thus, the observed pH effect (pKa approximately 9.2) likely reflects protonation equilibrium of the lysine residue, which would both raise E degrees and provide a proton for lowering the barrier for the N-O cleavage and for reduction of the [Cu4S(im)7OH]2+ to the fully reduced 4CuI active form for turnover.
0002-7863 (Print)0002-7863 (Linking)Journal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.
Keywords: Computer Simulation; Copper/*chemistry; Cross-Linking Reagents/*chemistry; Crystallography, X-Ray; Electron Spin Resonance Spectroscopy; Hydrogen-Ion Concentration; Kinetics; Ligands; Models, Molecular; Molecular Conformation; Oxidoreductases/*chemistry/*metabolism; Sulfides/*chemistry; Vibration

Boron trifluoride catalyzed polymerisation of 2-substituted-2-oxazolines in supercritical carbon dioxide - de Macedo, Carlota Veiga; Mara Soares da Silva; Casimiro, Teresa; Cabrita, Eurico J.; Aguiar-Ricardo, Ana (Green Chemistry, 2007)
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The chemistry of 6-hydroxyflavylium: zwitterionic base and p-quinoidal chalcones. A multiswitchable system operated by proton, electron and photon inputs - Jimenez, A.; Pinheiro, C; A. J. Parola; Maestri, M; Pina, F (Photochemical & Photobiological Sciences, 2007)
The complex network of chemical reactions of the compound 6-hydroxyflavylium perchlorate was studied by different techniques including UV-vis absorption spectrophotometry, stopped-flow, electrochemistry, and photochemistry. The network is characterized by the formation of a very reactive zwitterionic base as well as by p-quinoidal chalcones. The trans-chalcone is metastable in very acidic solutions and exhibits reversible redox reactions, allowing to introduce for the first time in the flavylium network an electrochemical input.
ISI Document Delivery No.: 152RVTimes Cited: 6 Cited Reference Count: 43 Jimenez, Antonio Pinheiro, Carlos Parola, A. Jorge Maestri, Mauro Pina, Fernando Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013 Parola, A. Jorge/0000-0002-1333-9076; Royal soc chemistry Cambridge

Effect of SDS micelles on the reactivity of 4 '-methoxyflavylium ion: A stopped-flow and photochemical study - A. J. Parola; P Pereira; Pina, F; Maestri, M (Journal of Photochemistry and Photobiology a-Chemistry, 2007)
The network of chemical reactions of the compound 4'-methoxyflavylium was studied in the presence of SDS micelles, using stopped-flow, UV-vis absorption and flash photolysis techniques. The results were compared with analogous experiments carried out in water. The large stabilization of the flavylium cation onto the micelles was proven to be due to an increase in the rate of the dehydration reaction, rather than a decrease of the hydration reaction; the cis-trans isomerisation of the chalcone was not affected by the micelles. It is shown that SDS micelles can be considered as an external stimulus capable of changing the system from one state to another. (c) 2006 Elsevier B.V. All rights reserved.
ISI Document Delivery No.: 129NQTimes Cited: 5 Cited Reference Count: 67 Parola, A. Jorge Pereira, Pedro Pina, Fernando Maestri, Mauro Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013 Parola, A. Jorge/0000-0002-1333-9076; Elsevier science sa Lausanne

Efficient photochromism from the network of chemical reactions of 7,4 '-dihydroxyflavylium in CTAB micelles - Gomes, R; A. J. Parola; Laia, C. A. T.; Pina, F (Photochemical & Photobiological Sciences, 2007)
Differently from water,effcient photochromism with a strong colour contrast has been observed for the multistate compound 7,4'- dihydroxyflavylium in the presence of cetyl trimethylammonium bromide ( CTAB) micelles. Two states are responsible for the photochromism: trans-chalcone ( inside the micelle) in the dark, and flavylium cation, AH(+), ( in bulk water) upon irradiation. The kinetics of the system was characterized by. ash photolysis and pH jumps. Evidence that the photochemical process leading to AH(+) occurs in the micelle was achieved. The best colour contrast is obtained at pH = 1.5, from a solution practically colourless in the dark, to an intense yellow upon irradiation ( quantum yield 0.4). The system is completely reversible with a lifetime of 38 min at room temperature, and exhibits a reasonable stability. A kinetic model capable of fitting the data from thermal entrance of the compound into the micelle, its ejection to bulk water upon irradiation and quantum yields of the photochemical process is proposed.
ISI Document Delivery No.: 203PHTimes Cited: 21 Cited Reference Count: 22 Gomes, Raquel Parola, A. Jorge Laia, Cesar A. T. Pina, Fernando Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013 Parola, A. Jorge/0000-0002-1333-9076; Royal soc chemistry Cambridge

Identification of 7,4 '-dihydroxy-5-methoxyflavylium in "Dragon's blood": To be or not to be an anthocyanin - Melo, MJ; M. Sousa; A. J. Parola; de Melo, JSS; Catarino, F.; Marcalo, J.; Pina, F (Chemistry-a European Journal, 2007)
The compound 7,4'-dihydroxy-5-methoxyflavylium (dracoflavylium) was identified as the major red colorant in samples of the resin "dragon's blood", extracted from the tree Dracaena draco. The complex network of reversible chemical reactions that dracoflavylium undergoes in aqueous solution is fully described; for the first time, all the equilibrium constants that enable a complete characterisation of the system have been obtained (K'(a)=1.6 x 10(-4), K-a1 = 1.0 x 10(-4), K-a2 = 3.2 x 10(-8), K-Ct1 = 1.0 x 10(-7), K-Ct2 = 1.3 x 10(-10)). It is concluded that the red colour is due to a stable quinoid base, A, which is the major species at pH 4-7. It is further shown that this compound does not fit the commonly accepted definitions of anthocyanidin nor 3-deoxyanthocyanidin. Similarly to synthetic flavylium salts, the natural compound 7,4'-dihydroxy-5-methoxyflavylium gives rise to several species (multistate system) reversibly interconverted by external stimuli, such as pH.
ISI Document Delivery No.: 136KGTimes Cited: 18 Cited Reference Count: 46 Melo, Maria J. Sousa, Micaela Parola, A. Jorge Seixas de Melo, J. Srgio Catarino, Fernando Marcalo, Joaquim Pina, Fernando Melo, Maria Joao/C-8594-2011; Seixas de Melo, Sergio/A-5388-2008; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013; Marcalo, Joaquim/J-5476-2013 Seixas de Melo, Sergio/0000-0001-9708-5079; Parola, A. Jorge/0000-0002-1333-9076; Marcalo, Joaquim/0000-0001-7580-057X Wiley-v c h verlag gmbh Weinheim

Multistate reaction kinetics of 6-hydroxy-4 '-(dimethylamino)flavylium driven by pH. A stopped-flow study - Laia, C. A. T.; A. J. Parola; Folgosa, F.; Pina, F (Organic & Biomolecular Chemistry, 2007)
The synthetic flavylium salt 6-hydroxy-4'-(dimethylamino)flavylium hexa. uorophosphate displays a set of pH-driven chemical reactions in aqueous solutions, involving the formation of hemiketal species and chalcones with cis and trans configurations. Such reactions were studied by steady-state and transient UV-Vis spectroscopy and by stopped-flow techniques. A novel and more generalized kinetic scheme is developed, in order to take account of possible acid/base pairs that occur in the network of chemical reactions as the pH is changed. It is found that the formation of the hemiketal species by hydration of the. avylium is slow, and it is not possible to isolate each process that leads to the formation of the cis-chalcone ( hydration and tautomerization reactions). The cis/trans isomerization reaction of cis-chalcone is slow, and the system takes several hours to reach equilibrium after a pH jump at room temperature. In basic conditions, negatively charged trans-chalcones are dominant. Comparison with other. avylium compounds shows that the hydration process is affected mainly by the amino group, while the hydroxyl group influences the tautomerization and isomerization reactions.
ISI Document Delivery No.: 116TXTimes Cited: 9 Cited Reference Count: 33 Laia, Cesar A. T. Parola, A. Jorge Folgosa, Filipe Pina, Fernando Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Folgosa, Filipe/F-7640-2012; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013 Folgosa, Filipe/0000-0001-5879-151X; Parola, A. Jorge/0000-0002-1333-9076; Royal soc chemistry Cambridge

Photochromism of 7-(N, N-diethylamino) 4 '-hydroxyflavylium in a water-ionic liquid biphasic system - Pina, F; A. J. Parola; Melo, MJ; Laia, C. A. T.; C. A. M. Afonso (Chemical Communications, 2007)
Photochromism of trans-4-(N,N-diethylamino)-2,4'-dihydroxychalcone, with formation of the photoproduct 7-(N,N-diethylamino)-4'-hydroxyflavylium, is promoted in the ionic liquid phase of a water/[bmim][PF6] biphasic system.
ISI Document Delivery No.: 162RYTimes Cited: 11 Cited Reference Count: 14 Pina, Fernando Parola, A. Jorge Melo, Maria Joao Laia, Cesar A. T. Afonso, Carlos A. M. Melo, Maria Joao/C-8594-2011; Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013 Parola, A. Jorge/0000-0002-1333-9076; Royal soc chemistry Cambridge

Promoting photochromism on flavylium derived 2-hydroxychalcones in aqueous solutions by addition of CTAB micelles - Gomes, R; A. J. Parola; Laia, C. A. T.; Pina, F (Journal of Physical Chemistry B, 2007)
A strategy to obtain photochromism from the network of chemical reactions originated by flavyliurn compounds in solution is described. This strategy is particularly useful for flavyliurn salts bearing amino groups which give rise to a variety of beautiful colors but lack photochemistry in water. The trans-chalcone of 7-(N,Ndiethylamino)-4'-hydroxyflavylium interacts strongly with CTAB micelles defining a yellow dark state. Upon irradiation, the system switches to a pink-red state emerging from the flavylium cation that is formed inside the micelle and ejected to the bulk aqueous phase. The photochemical product reverts back to the transchalcone adduct with the micelle in the dark. The thermodynamics as well as the kinetics of the photochromic system were studied in detail. The best color contrast is obtained at pH = 4.25 with (D = 0.001 and a recovery lifetime of approximately 3 h. This photochromic system works with no need of changing the pH, which constitutes an important improvement over previously described systems dependent on pH jumps.
ISI Document Delivery No.: 221XKTimes Cited: 11 Cited Reference Count: 22 Gomes, Raquel Parola, A. Jorge Laia, Cesar A. T. Pina, Fernando Laia, Cesar/C-8651-2011; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013 Parola, A. Jorge/0000-0002-1333-9076; Amer chemical soc Washington

Revisiting Perkin's dye(s): the spectroscopy and photophysics of two new mauveine compounds (B2 and C) - de Melo, JS; Takato, S.; M. Sousa; Melo, MJ; A. J. Parola (Chemical Communications, 2007)
Two new components have been identified in an early sample prepared according to the original recipe of Perkin, and perhaps even by Perkin himself around 1860 - a new isomer of Perkin's mauveine B (designated as mauveine B2) together with a new mauveine compound (mauveine C) - and these compounds were synthesized again using starting materials chosen to reproduce Perkin's original synthesis and isolated by HPLC-DAD, identified by H-1 NMR, MS and their spectroscopic (UV/Vis and emission) and photophysical behaviour investigated.
ISI Document Delivery No.: 180WLTimes Cited: 16 Cited Reference Count: 20 de Melo, J. Seixas Takato, S. Sousa, M. Melo, M. J. Parola, A. J. Melo, Maria Joao/C-8594-2011; Parola, A. Jorge/F-4048-2010; Seixas de Melo, Sergio/A-5388-2008; REQUIMTE, AL/H-9106-2013 Parola, A. Jorge/0000-0002-1333-9076; Seixas de Melo, Sergio/0000-0001-9708-5079; Royal soc chemistry Cambridge

Simplifying sample handling for protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry - Cordeiro, Francisco M.; Carreira, Ricardo J.; Rial-Otero, Raquel; Rivas, M. Gabriela; Moura, Isabel; Capelo, Jose-Luis (Rapid Communications in Mass Spectrometry, 2007)
An ultrasonic bath, an ultrasonic probe and a sonoreactor were used to speed up the kinetics of the reactions involved in each step of the sample handling for in-gel protein identification by peptide mass fingerprint, PMF, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The following steps were successfully accelerated using ultrasonic energy: gel washing, protein reduction, and protein alkylation. As a result, a reduction comprising 80% to 90% of the total time involved in the classic approach was achieved. In addition the sample handling was also drastically simplified. The number of peptides identified and the protein sequence coverage obtained for the new procedure were comparable to those obtained with the traditional sample treatment for the following protein standards: glycogen phosphorylase b, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor and alpha-lactalbumin. Finally, as a proof of the procedure, specific proteins were identified from complex protein mixtures obtained from three different sulphate- reducing bacteria: Desulfovibrio, desulfuricans G20, Desulfuvibrio gigas NCIB 9332, and Desulfuvibrio desulfuricans ATCC 27774. Copyright (c) 2007 John Wiley & Sons, Ltd.
Times Cited: 10

Substitution and solvent effects in the chalcones isomerization barrier of flavylium photochromic systems - Roque, A.; J. C. Lima; A. J. Parola; Pina, F (Photochemical & Photobiological Sciences, 2007)
Useful application of photochromic compounds as optical memories implies the existence of a large kinetic barrier between the forms interconverted by light. In the case of flavylium salts, the ground state isomerization barrier between the photoisomerizable chalcone isomers is shown to correlate with the electron donating ability of the substituents, measured by their effects in the H-1 NMR chemical shifts of the aromatic protons. Substitution with electron donating groups in ring A lowers the barrier while substitution at ring B has the opposite effect. However, in water, the observed increase is higher than expected in the case of compound 4', 9-dihydroxychalcone when compared with the analogous 4'-dimethylamino-9-hydroxychalcone, containing a better electron donating group in the same position. Our interpretation is that the water network is providing an efficient pathway to form tautomers. In acetonitrile, unlike water, the expected order is indeed observed: E-a( 4', 9-dihydroxychalcone) = 60 kJ mol(-1) < E-a ( 4'-dimethylamino-9-hydroxychalcone) = 69 kJ mol(-1).
ISI Document Delivery No.: 152RVTimes Cited: 12 Cited Reference Count: 24 Roque, Ana Lima, Joao Carlos Parola, A. Jorge Pina, Fernando Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013; Lima, Joao/F-3658-2010 Parola, A. Jorge/0000-0002-1333-9076; Lima, Joao/0000-0003-0528-1967 Royal soc chemistry Cambridge

Synthesis and photophysical properties of dansyl-based polyamine ligands and their Zn(II) complexes - A. J. Parola; J. C. Lima; Pina, F; Pina, J; de Melo, JS; Soriano, C; Garcia-Espana, E; Aucejo, R; Alarcon, J. (Inorganica Chimica Acta, 2007)
The synthesis, potentiometric studies and photophysical properties of two new polyamine ligands (L1 and L2) possessing the dansyl chromophore were studied in aqueous 0.15 M NaCl. The compounds show the absorption and emissions bands characteristic of the dansylamide fluorophore and both present intramolecular excited state proton transfer at intermediate pH ranges. One of the ligands (L2) strongly coordinates Zn(II) leading to fluorescence quenching. A model compound (L3) of the dansyl moiety was also investigated. (c) 2006 Elsevier B.V. All rights reserved.
ISI Document Delivery No.: 138LRTimes Cited: 12 Cited Reference Count: 44 Parola, A. Jorge Lima, Joao Carlos Pina, Fernando Pina, Joao de Melo, Joao Seixas Soriano, Conxa Garcia-Espana, Enrique Aucejo, Ricardo Alarcon, Javier Seixas de Melo, Sergio/A-5388-2008; Pina, Fernando/C-8161-2011; Parola, A. Jorge/F-4048-2010; REQUIMTE, AL/H-9106-2013; Pina, Joao /H-9948-2013; Lima, Joao/F-3658-2010 Seixas de Melo, Sergio/0000-0001-9708-5079; Parola, A. Jorge/0000-0002-1333-9076; Lima, Joao/0000-0003-0528-1967 Elsevier science sa Lausanne

Marginal CO2 abatement costs for the Portuguese energy system - scenarios analysis for 2030 - Cleto, João; Simões, Sofia; Patrícia Fortes; Júlia Seixas (9th International Conference Energy for a Clean Environment, 2007)

Assessing Human and Technological Dimensions in Virtual Team's Operational Competences - Sampaio, José; Moniz, António B. (IET, 2007)
Cognitive task automation may lead to over trust, complacency and loss of the necessary work environment situation awareness. This is a major constraint in complex work organizations teamwork, ending up into an operational gap, between system developments and its understanding and usability, by operators. This document presents a summary of the main results of author’s research on operational decision processes and occupational competences, applied to the air traffic control operational reality. Introducing a human/technological complementary approach to virtual team’s conceptualisation, the results show there is a dimension to be followed in human/machine integration, which stands beyond interface design, and calls for a deeper human comprehension of technological agent’s structure and functionalities, which will, ultimately, require the development of an operational cognitive framework, where work processes and technological behaviour are integrated in professional competences, as he two faces of the same coin.

trans-Acting Factors and cis Elements Involved in Glucose Repression of Arabinan Degradation in Bacillus subtilis. - Inácio, José Manuel; Isabel de Sá-Nogueira (Journal of Bacteriology, 2007)
In Bacillus subtilis, the synthesis of enzymes involved in the degradation of arabinose-containing polysaccharides is subject to carbon catabolite repression (CCR). Here we show that CcpA is the major regulator of repression of the arabinases genes in the presence of glucose. CcpA acts via binding to one cre each in the promoter regions of the abnA and xsa genes and to two cres in the araABDLMNPQ-abfA operon. The contributions of the coeffectors HPr and Crh to CCR differ according to growth phase. HPr dependency occurs during both exponential growth and the transitional phase, while Crh dependency is detected mainly at the transitional phase. Our results suggest that Crh synthesis may increase at the end of exponential growth and consequently contribute to this effect, together with other factors.
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Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon - Franco, Irina Saraiva; Mota, Luís Jaime; Soares, Cláudio Manuel; Isabel de Sá-Nogueira (Nucleic Acids Research, 2007)
In the absence of arabinose, the AraR transcription factor represses the expression of genes involved in the utilization of arabinose, xylose and galactose in Bacillus subtilis. AraR exhibits a chimeric organization: the N-terminal DNA-binding region belongs to the GntR family and the C-terminal effector-binding domain is homologous to the GalR/LacI family. Here, the AraR–DNA-binding interactions were characterized in vivo and in vitro. The effect of residue substitutions in the AraR N-terminal domain and of base-pair exchanges into an AraR–DNA-binding operator site were examined by assaying for AraR-mediated regulatory activity in vivo and DNA-binding activity in vitro. The results showed that residues K4, R45 and Q61, located in or near the winged-helix DNA-binding motif, were the most critical amino acids required for AraR function. In addition, the analysis of the various mutations in an AraR palindromic operator sequence indicated that bases G9, A11 and T16 are crucial for AraR binding. Moreover, an AraR mutant M34T was isolated that partially suppressed the effect of mutations in the regulatory cis-elements. Together, these findings extend the knowledge on the nature of AraR nucleoprotein complexes and provide insight into the mechanism that underlies the mode of action of AraR and its orthologues.
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Affinity chromatography: History, Perspectives, Limitations and Prospects - Roque, A. C. A.; Lowe, C. R. (Affinity Chromatography: Methods and Protocols, 2007)
Biomolecule separation and purification has until very recently steadfastly remained one of the more empirical aspects of modern biotechnology. Affinity chromatography, one of several types of adsorption chromatography, is particularly suited for the efficient isolation of biomolecules. This technique relies on the adsorbent bed material that has biological affinity for the substance to be isolated. This review is intended to place affinity chromatography in historical perspective and describe the current status, limitations and future prospects for the technique in modern biotechnology.

Affinity-based methodologies and ligands for antibody purification: Advances and perspectives - Roque, Ana C A; Silva, Cláudia S.O.; Taipa, M. Ângela (Journal of Chromatography A, 2007)
Many successful, recent therapies for life-threatening diseases such as cancer and rheumatoid arthritis are based on the recognition between native or genetically engineered antibodies and cell-surface receptors. Although naturally produced by the immune system, the need for antibodies with unique specificities and designed for single application, has encouraged the search for novel antibody purification strategies. The availability of these products to the end-consumer is strictly related to manufacture costs, particularly those attributed to downstream processing. Over the last decades, academia and industry have developed different types of interactions and separation techniques for antibody purification, affinity-based strategies being the most common and efficient methodologies. The affinity ligands utilized range from biological to synthetic designed molecules with enhanced resistance and stability. Despite the successes achieved, the purification “paradigm” still moves interests and efforts in the continuous demand for improved separation performances. This review will focus on recent advances and perspectives in antibody purification by affinity interactions using different techniques, with particular emphasis on affinity chromatography.
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Keywords: Affinity purification; Antibodies; Bioengineered ligands; Biospecific ligands; Combinatorial Chemistry; Intelligent design; Pseudobiospecific ligands; Synthetic mimic affinity ligands

Analysis of the generators of epileptic activity in early-onset childhood benign occipital lobe epilepsy - Alberto Leal; Sofia Nunes; Ana I Dias; José P Vieira; Ana Moreira,; Eulália Calado (Clinical Neurophysiology, 2007)

Analysis of the generators of epileptic activity in early-onset childhood benign occipital lobe epilepsy - Leal, A; Nunes, S; Dias, AI; Vieira, JP; Ana, Moreira; Calado, E (Clinical Neurophysiology, 2007)

Brain Mapping of Epileptic Activity in a Case of Idiopathic Occipital Lobe Epilepsy (Panayiotopoulos Syndrome) - Alberto Leal; Sofia Nunes; António Martins; Mário Forjaz Secca; Constança Jordão (Epilepsia, 2007)

Brain Mapping of Epileptic Activity in a Case of Idiopathic Occipital Lobe Epilepsy (Panayiotopoulos Syndrome) - Leal, A; Nunes, S; Martins, A; Secca, MF; Jordão, C (Epilepsia, 2007)

The collaborative work concept and the information systems support: perspectives for and from manufacturing industry - Moniz, António B. (Technikfolgenabschätzung – Theorie und Praxis, 2007)
Most of the discussion and controversy on organisation of work concepts has been referenced to the manufacturing industry along the 20th century: it started with the concept of “scientific management” from Taylor, and continued with the new ideas on the importance of human factors as Mayo pointed out in the 1930s. Immediately after the 2nd World War Friedmann studied the human problems related to new manufacturing technologies and automation. And the late 1950 and 1960s were decades of strong debate on the socio-technics with the research at Tavistock Institute of London and the emergence of national programmes on new forms of work organisation. At the end of the last century the concept of collaborative work was developed together with the definition(s) of information systems and organisational design. However, the interest came from other production activities, like the services. This article analyses the approaches developed on these debates on the collaborative work and information system and its application to the manufacturing industry.

Development and Validation of an HPLC/UV Method for Quantification of Bioactive Peptides in Fermented Milks - Ferreira, Isabel M. P. L. V. O.; Eça, Rosário; Pinho, Olívia; Tavares, Pedro; Pereira, Alice; Roque, Ana Cecília (Journal of Liquid Chromatography & Related Technologies, 2007)
The simultaneous separation and quantification of two casein peptides {(IPP}, {VPP)} presenting potent inhibitory activity of angiotensin-converting-enzyme {(ACE)} and casein in fermented milks was developed. Gradient elution was carried out at a flow-rate of 1 {mL/min}, using a mixture of two solvents. Solvent A was 0.1% TFA in water and solvent B was acetonitrile-water-trifluoracetic acid 95:5:0.1. The effluent was monitored by UV detector at 214 nm. Calibration curves were constructed in the interval of 0.01-1.0 {mg/mL} for VPP, 0.005-1.0 {mg/mL} for IPP, and 0.05-3.0 {mg/mL} for casein. R2 invariably exceeded 0.999. The detection limits were 0.004 for VPP, 0.002 {mg/mL} for IPP, and 0.02 {mg/mL} for casein. Repeatability of the method was evaluated by six consecutive injections of two standard solutions containing VPP, IPP, and casein. The RSD values for concentration were all below 5.08%. Recovery studies were carried out to determine the accuracy of the method. Recoveries ranged between 88 and 98.2%. The methodology was applied, not only, for the monitorization of VPP, IPP, and casein in commercial fermented milks labeled as presenting antihypertensive properties, but also, in milk with different degrees of fermentation by L. Helveticus, and in other commercial functional fermented milks, such as, those presenting cholesterol lowering properties.
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Keywords: Casein; Fermented Milks; ACE-Inhibitory Peptides; Rp-Hplc

Fluorescence ratio imaging microscopy shows decreased access of vancomycin to cell wall synthetic sites in vancomycin-resistant Staphylococcus aureus - P. M. Pereira; S. R. Filipe; A. Tomasz; M. G. Pinho (Antimicrob Agents Chemother, 2007)

A new look at the fractional Brownian motion definition - Ortigueira, M.D.; Batista, A.G. (Conference on Multibody Systems, Nonlinear Dynamics and Control (MSNDC). : ASME IDETC07, 2007)
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A Non Integer Order Quantum Derivative - Ortigueira, M. (Symposium on Applied Fractional Calculus (SAFC07), 2007)
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Origin of glassy dynamics in a liquid crystal studied by broadband dielectric and specific heat spectroscopy - A. R. Brás; M. Dionísio; H. Huth; C. Shick; A. Schoenhals (Physical Review E, 2007)

Parecer sobre a oportunidade de investimento turístico-imobiliário na Ilha de São Vicente em Cabo Verde - Amado, Miguel; Others (GEOTPU, 2007)

Parecer sobre Habitats da Rede Natura 2000 na Área do Rego Travesso – Seixal - Amado, Miguel; et al (GEOTPU, 2007)

A participação da população no processo de ordenamento das áreas de paisagem protegida, - Amado, Miguel (olóquio Património Paisagístico: os caminhos da transversalidade, 2007)

Potential dementia biomarkers based on the time-varying microstructure of sleep EEG spindles - Ktonas, PY; Golemati, S.; Xanthopoulos, P.; Sakkalis, V.; Ortigueira, M.D.; Tsekou, H.; Zervakis, M.; Paparrigopoulos, T.; Soldatos, CR (2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. :2464–2467, 2007)
The time-varying microstructure of sleep EEG spindles may have clinical significance in dementia studies. In this work, the sleep spindle is modeled as an AM-FM signal and parameterized in terms of six parameters, three quantifying the instantaneous envelope (IE) and three quantifying the instantaneous frequency (IF) of the spindle model. The IE and IF waveforms of sleep spindles from patients with dementia and normal controls were estimated using the time-frequency technique of complex demodulation (CD). Sinusoidal curve-fitting using a matching pursuit (MP) approach was applied to the IE and IF waveforms for the estimation of the six model parameters. Specific differences were found in sleep spindle instantaneous frequency dynamics between spindles from dementia subjects and spindles from controls.

Potentially Biodegradable Polymers based on α- or β-Pinene and Sugar Derivatives or Styrene, Obtained under Normal Conditions and Microwave Irradiation - M. T. Barros; K. T. Petrova; A. M. Ramos (Eur. J. Org. Chem., 2007)

Prediction of 1H NMR coupling constants with associative neural networks trained for chemical shifts - Y. Binev; M.M. Marques; J. Aires-de-Sousa (J. Chem. Inf. Model., 2007)

Rationally designed ligands for use in Affinity Chromatography: An artificial Protein L - Roque, A. C. A.; Lowe, C. R. (Affinity Chromatography: Methods and Protocols, 2007)
Synthetic affinity ligands can circumvent the drawbacks of natural immunoglobulin (Ig)-binding proteins by imparting resistance to chemical and biochemical degradation and to in situ sterilization, as well as ease and low cost of production. Protein L (PpL), isolated from Peptostreptococcus magnus strains, interacts with the Fab (antigen-binding fragment) portion of Igs, specifically with kappa light chains, and represents an almost universal ligand for the purification of antibodies. The concepts of rational design and solid-phase combinatorial chemistry were used for the discovery of a synthetic PpL mimic affinity ligand. The procedure presented in this chapter represents a general approach with the potential to be applied to different systems and target proteins.

Redox-linked conformational changes of a multiheme cytochrome from Geobacter sulfurreducens - Leonor Morgado; Bruix, Marta; Londer, Yuri Y.; Pokkuluri, P.R.; Schiffer, Marianne; Salgueiro, C.A. (Biochemical and Biophysical Research Communications, 2007)
Multiheme c-type cytochromes from members of the Desulfovibrionacea and Geobactereacea families play crucial roles in the bioenergetics of these microorganisms. Thermodynamic studies using NMR and visible spectroscopic techniques on tetraheme cytochromes c3 isolated from Desulfovibrio spp. and more recently on a triheme cytochrome from Geobacter sulfurreducens showed that the properties of each redox centre are modulated by the neighbouring redox centres enabling these proteins to perform energy transduction and thus contributing to cellular energy conservation. Electron/proton transfer coupling relies on redox-linked conformational changes that were addressed for some multiheme cytochromes from the comparison of protein structure of fully reduced and fully oxidised forms. In this work, we identify for the first time in a multiheme cytochrome the simultaneous presence of two different conformations in solution. This was achieved by probing the different oxidation stages of a triheme cytochrome isolated from G. sulfurreducens using 2D-NMR techniques. The results presented here will be the foundations to evaluate the modulation of the redox centres properties by conformational changes that occur during the reoxidation of a multiheme protein.

Regulação da Transcrição em Procariontes. - Isabel de Sá-Nogueira (O mundo do RNA: novos desafios e perspectivas futuras, 2007)

Research and Development expenditure in the business sector as indicator of knowledge economy: the Portuguese experience - Nuno Boavida; António Brandão Moniz (IET Working Papers Series, 2007)

Revisiting the Initial Conditions Problem in Fractional Linear Systems - Ortigueira, M.; Coito, F. (Symposium on Applied Fractional Calculus (SAFC07), 2007)
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Riesz Potentials as Centred Derivatives - Ortigueira, M.D. (Advances in Fractional Calculus, 2007)
Generalised fractional centred differences and derivatives are studied in this Chapter. These generalise to real orders the existing ones valid for even and odd positive integer orders. For each one, suitable integral formulations are presented. The limit computation inside the integrals leads to generalisations of the Cauchy derivative. Their computations using a special path lead to the well known Riesz potentials. A study for coherence is done by applying the definitions to functions with Fourier transform. The existence of inverse Riesz potentials is also studied.

A Spectroscopic Study of the Temperature Induced Modifications on Ferredoxin Folding and Iron−Sulfur Moieties - Todorovic, Smilja; Sónia S. Leal; Salgueiro, C.A.; Zebger, Ingo; Hildebrandt, Peter; Murgida, Daniel H.; Gomes, C.M. (Biochemistry, 2007)
Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods. FTIR and visible CD were used for assessing changes of the secondary structure and coarse alterations of the [3Fe4S] and [4Fe4S] cluster moieties, respectively. Fine details of the disassembly of the metal centers were revealed by paramagnetic NMR and resonance Raman spectroscopy. Overall, thermally induced unfolding of AaFd is initiated with the loss of α-helical content at relatively low temperatures (Tapp (m) ~ 44 °C), followed by the disruption of both iron−sulfur clusters (Tapp (m) ~ 53−60 °C). The degradation of the metal centers triggers major structural changes on the protein matrix, including the loss of tertiary contacts (Tapp (m) ~ 58 °C) and a change, rather than a significant net loss, of secondary structure (Tapp (m) ~ 60 °C). This latter process triggers a secondary structure reorganization that is consistent with the formation of a molten globule state. The combined spectroscopic approach here reported illustrates how changes in the metalloprotein organization are intertwined with disassembly of the iron−sulfur centers, denoting the conformational interplay of the protein backbone with cofactors.

Вплив природи, полярності та сукупність електродів на процес формування покриттів карбонітридів електрохімічним методом в ацетонитрилі - Чеховський А.О., Томила Т.В., Рагуля А.В., Тимофєєва І.І., Іванчук А.А., Биков Д.А., Лобунець Т.Ф., Барабаш В.А. (Сучасні проблеми фізичного матеріалознавства, 2007)

Application of nanofiltration to re-use the sharpless asymmetric dihydroxylation catalytic system - Ferreira, Frederico Castelo; Branco, Luis C.; Kaushal, Kishor Vernia A.; Crespo, Joao G.; Afonso, Carlos A. M. (Tetrahedron-Asymmetry, 2007)
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Times Cited: 9 Branco, Luis/C-6426-2013; Crespo, Joao/D-8719-2013; REQUIMTE, AL/H-9106-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 9

The art of CO2 for art conservation: a green approach to antique textile cleaning - Sousa, Micaela; Melo, Maria Joao; Casimiro, Teresa; Aguiar-Ricardo, Ana (Green Chemistry, 2007)
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Times Cited: 11 Melo, Maria Joao/C-8594-2011; Aguiar-Ricardo, Ana/C-3286-2011; Casimiro, Teresa/D-2365-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; REQUIMTE, LAQV/N-9835-2013 Melo, Maria Joao/0000-0001-7393-6801; Aguiar-Ricardo, Ana/0000-0002-2193-1440; Casimiro, Teresa/0000-0001-9405-6221; 11

The Collaborative Work Concept and the Information Systems Support: Perspectives for and from Manufacturing Industry - António Moniz (University Library of Munich, Germany, 2007)
Most of the discussion and controversy on organisation of work concepts has been referenced to the manufacturing industry along the 20th century: it started with the concept of “scientific management” from Taylor, and continued with the new ideas on the importance of human factors as Mayo pointed out in the 1930s. Immediately after the 2nd World War Friedmann studied the human problems related to new manufacturing technologies and automation. And the late 1950 and 1960s were decades of strong debate on the socio-technics with the research at Tavistock Institute of London and the emergence of national programmes on new forms of work organisation. At the end of the last century the concept of collaborative work was developed together with the definition(s) of information systems and organisational design. However, the interest came from other production activities, like the services. This article analyses the approaches developed on these debates on the collaborative work and information system and its application to the manufacturing industry.
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Keywords: Collaborative Work; Information Systems; Manufacturing

A comparative study on absorption and selectivity of organic vapors by using ionic liquids based on imidazolium, quaternary ammonium, and guanidinium cations - Kulkarni, Prashant S.; Branco, Luis C.; Crespo, Joao G.; Afonso, Carlos A. M. (Chemistry-a European Journal, 2007)
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Times Cited: 20 Branco, Luis/C-6426-2013; Crespo, Joao/D-8719-2013; REQUIMTE, AL/H-9106-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 20

Comparison of physicochemical properties of new ionic liquids based on imidazolium, quaternary ammonium, and guanidinium cations - Kulkarni, Prashant S.; Branco, Luis C.; Crespo, Joao G.; Nunes, M. Cristiana; Raymundo, Anabela; Afonso, Carlos A. M. (Chemistry-a European Journal, 2007)
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Times Cited: 83 Branco, Luis/C-6426-2013; Crespo, Joao/D-8719-2013; REQUIMTE, AL/H-9106-2013; REQUIMTE, PHOTO/M-4229-2013; REQUIMTE, CENG/M-4555-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; Nunes, Cristiana/N-6951-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; Nunes, Cristiana/0000-0002-1281-8606; 83

Correlating EPR and X-ray structural analysis of arsenite-inhibited forms of aldehyde oxidoreductase - Thapper, Anders; Boer, D. R.; Brondino, Carlos D.; Moura, Jose J. G.; Romao, Maria J. (Journal of Biological Inorganic Chemistry, 2007)
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Times Cited: 6 Thapper, Anders/A-6728-2010; Romao, Maria/A-4115-2013; Moura, Jose/D-6426-2013; REQUIMTE, AL/H-9106-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; 6

Crystal structure of the 16 heme cytochrome from Desulfovibrio gigas: A glycosylated protein in a sulphate-reducing bacterium - Santos-Silva, Teresa; Dias, Joao Miguel; Dolla, Alain; Durand, Marie-Claire; Goncalves, Luisa L.; Lampreia, Jorge; Moura, Isabel; Romao, Maria Joao (Journal of Molecular Biology, 2007)
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Times Cited: 9 Romao, Maria/A-4115-2013; Lampreia, Jorge/A-9927-2012; REQUIMTE, AL/H-9106-2013; Santos-Silva, Teresa/D-2050-2013; REQUIMTE, SMB/M-5694-2013; Moura, Isabel/D-6339-2013; REQUIMTE, UCIBIO/N-9846-2013 Romao, Maria/0000-0002-3004-0543; Lampreia, Jorge/0000-0001-8846-0041; Moura, Isabel/0000-0003-0971-4977; 9

Development and validation of an HPLC/UV method for quantification of bioactive peptides in fermented milks - Ferreira, Isabel M. P. L. V. O.; Eca, Rosario; Pinho, Olívia; Tavares, Pedro; Pereira, Alice; Roque, Ana Cecilia (JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2007)
The simultaneous separation and quantification of two casein peptides (IPP, VPP) presenting potent inhibitory activity of angiotensin-converting-enzyme (ACE) and casein in fermented milks was developed. Gradient elution was carried out at a flow-rate of 1 mL/min, using a mixture of two solvents. Solvent A was 0.1% TFA in water and solvent B was acetonitrile-water-trifluoracetic acid 95:5:0.1. The effluent was monitored by UV detector at 214 nm. Calibration curves were constructed in the interval of 0.01-1.0 mg/mL for VPP, 0.005-1.0 mg/mL for IPP, and 0.05-3.0 mg/mL for casein. R 2 invariably exceeded 0.999. The detection limits were 0.004 for VPP, 0.002 mg/mL for IPP, and 0.02 mg/mL for casein. Repeatability of the method was evaluated by six consecutive injections of two standard solutions containing VPP, IPP, and casein. The RSD values for concentration were all below 5.08%. Recovery studies were carried out to determine the accuracy of the method. Recoveries ranged between 88 and 98.2%. The methodology was applied, not only, for the monitorization of VPP, IPP, and casein in commercial fermented milks labeled as presenting anti hypertensive properties, but also, in milk with different degrees of fermentation by L Helveticus, and in other commercial functional fermented milks, such as, those presenting cholesterol lowering properties.
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Dynamics of short as compared with long poly(acrylic acid) chains hydrophobically modified with pyrene, as followed by fluorescence techniques - de Melo, J. Seixas; Costa, Telma; Francisco, Alexandra; Macanita, Antonio L.; Gago, Sandra; Goncalves, Isabel S. (Physical Chemistry Chemical Physics, 2007)
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Times Cited: 23 Seixas de Melo, Sergio/A-5388-2008; Macanita, Antonio/H-9284-2012; Goncalves, Isabel/H-3742-2013; REQUIMTE, PHOTO/M-4229-2013; Gago, Sandra/H-5413-2012; REQUIMTE, LAQV/N-9835-2013 Seixas de Melo, Sergio/0000-0001-9708-5079; Macanita, Antonio/0000-0003-0976-6654; Goncalves, Isabel/0000-0002-2836-7715; Gago, Sandra/0000-0003-0221-8508; 23

Effect of ionic liquids on human colon carcinoma HT-29 and CaCo-2 cell lines - Frade, Raquel F. M.; Matias, Ana; Branco, Luis C.; Afonso, Carlos A. M.; Duarte, Catarina M. M. (Green Chemistry, 2007)
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Times Cited: 42 Branco, Luis/C-6426-2013; REQUIMTE, AL/H-9106-2013; Matias, Ana/J-2387-2013; Frade, Raquel/J-4555-2013; REQUIMTE, PHOTO/M-4229-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Branco, Luis/0000-0003-2520-1151; Matias, Ana/0000-0002-4888-9414; Frade, Raquel/0000-0002-1200-2447; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; 45

Efficient catalyst reuse by simple dissolution in non-conventional media - Afonso, Carlos A. M.; Branco, Luis C.; Candeias, Nuno R.; Gois, Pedro M. P.; Lourenco, Nuno M. T.; Mateus, Nuno M. M.; Rosa, Joao N. (Chemical Communications, 2007)
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Times Cited: 39 Lourenco, Nuno/K-5088-2012; Branco, Luis/C-6426-2013; REQUIMTE, AL/H-9106-2013; Rosa, Joao/I-6129-2013; REQUIMTE, PHOTO/M-4229-2013; Mateus Afonso, Carlos Alberto/M-7833-2013; REQUIMTE, LAQV/N-9835-2013; gois, pedro/A-9251-2014; PTMS, RNEM/C-1589-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; iMed.ULisboa, BioOrgChem /B-4209-2014 Lourenco, Nuno/0000-0001-9329-2724; Branco, Luis/0000-0003-2520-1151; Rosa, Joao/0000-0001-5165-8820; Mateus Afonso, Carlos Alberto/0000-0002-7284-5948; gois, pedro/0000-0002-7698-630X; 40

Evidence for a dual binding mode of dockerin modules to cohesins - Carvalho, Ana Luisa; Dias, Fernando M. V.; Nagy, Tibor; Prates, Jose A. M.; Proctor, Mark R.; Smith, Nicola; Bayer, Edward A.; Davies, Gideon J.; Ferreira, Luis M. A.; Romao, Maria J.; Fontes, Carlos M. G. A.; Gilbert, Harry J. (Proceedings of the National Academy of Sciences of the United States of America, 2007)
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Times Cited: 56 Davies, Gideon/A-9042-2011; Dias, Fernando/I-4861-2012; Carvalho, Ana Luisa/G-5638-2011; Romao, Maria/A-4115-2013; REQUIMTE, AL/H-9106-2013; Prates, Jose/K-9934-2013; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013 Davies, Gideon/0000-0002-7343-776X; Dias, Fernando/0000-0001-8109-2063; Carvalho, Ana Luisa/0000-0002-3824-0240; Romao, Maria/0000-0002-3004-0543; Prates, Jose/0000-0003-1032-5987; 57

Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purification, crystallization and preliminary X-ray analysis - Coelho, Catarina; Gonzalez, Pablo J.; Trincao, Jose; Carvalho, Ana L.; Najmudin, Shabir; Hettman, Thomas; Dieckman, Stephan; Moura, Jose J. G.; Moura, Isabel; Romao, Maria J. (Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 2007)
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Times Cited: 8 Carvalho, Ana Luisa/G-5638-2011; Romao, Maria/A-4115-2013; Coelho, Catarina /D-2073-2013; Moura, Jose/D-6426-2013; Gonzalez, Pablo/D-8200-2013; REQUIMTE, AL/H-9106-2013; REQUIMTE, SMB/M-5694-2013; Moura, Isabel/D-6339-2013; REQUIMTE, UCIBIO/N-9846-2013 Carvalho, Ana Luisa/0000-0002-3824-0240; Romao, Maria/0000-0002-3004-0543; Coelho, Catarina /0000-0003-1852-5406; Moura, Isabel/0000-0003-0971-4977; 6 8

The human carnitine acylcarnitine translocase (hCACT): Strategies for its heterologous expression, purification and crystallization - Fv, Ventura; Violante, S.; Gomes, C.; Carvalho, A. L.; Romao, M. J.; Gaspar, M. M.; Cruz, M. E. M.; Soveral, G.; Wanders, R. J.; Leandro, P.; de Almeida, Tavares V. (Journal of Inherited Metabolic Disease, 2007)
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Times Cited: 0 LNEG, Producao Cientifica/D-2212-2012; Romao, Maria/A-4115-2013; REQUIMTE, AL/H-9106-2013; Carvalho, Ana Luisa/G-5638-2011; REQUIMTE, SMB/M-5694-2013; REQUIMTE, UCIBIO/N-9846-2013; iMed.ULisboa, MetGen /B-5293-2014; iMed.ULisboa, iMed.ULisboa/C-6292-2014; Soveral, Graca/L-1550-2013; Leandro, Paula/A-8323-2014 Romao, Maria/0000-0002-3004-0543; Carvalho, Ana Luisa/0000-0002-3824-0240; Soveral, Graca/0000-0001-8487-110X; Leandro, Paula/0000-0002-2946-9342 1 0

Identification of 7,4 '-dihydroxy-5-methoxyflavylium in "Dragon's blood": To be or not to be an anthocyanin - Melo, Maria J.; Sousa, Micaela; A. Jorge Parola; Seixas de Melo, J. Srgio; Catarino, Fernando; Marcalo, Joaquim; Pina, Fernando (Chemistry-a European Journal, 2007)
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Times Cited: 20 Melo, Maria Joao/C-8594-2011; Seixas de Melo, Sergio/A-5388-2008; Pina, Fernando/C-8161-2011; REQUIMTE, AL/H-9106-2013; Marcalo, Joaquim/J-5476-2013; Group, Photochem&SC/M-4665-2013; REQUIMTE, PHOTO/M-4229-2013; Parola, A. Jorge/F-4048-2010; REQUIMTE, LAQV/N-9835-2013; PTMS, RNEM/C-1589-2014 Melo, Maria Joao/0000-0001-7393-6801; Seixas de Melo, Sergio/0000-0001-9708-5079; Pina, Fernando/0000-0001-8529-6848; Marcalo, Joaquim/0000-0001-7580-057X; Parola, A. Jorge/0000-0002-1333-9076; 21

Monitoring the structural alterations induced in P-lactoglobulin during ultrafiltration: learning from chemical and thermal denaturation phenomena - Portugal, Carla A. M.; Crespo, Joao G.; J. C. Lima (Journal of Membrane Science, 2007)
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A new look at the fractional Brownian motion definition - Ortigueira, M.D.; Batista, A.G. (Conference on Multibody Systems, Nonlinear Dynamics and Control (MSNDC), 2007)
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New quasi-orthogonal BCH-derived sequences for CDMA applications - Cesaltina N. E. Ricardo; Manuel D. Ortigueira; José António B. Gerald (European Transactions on Telecommunications, 2007)
Based on two methods recently proposed - the Ranging Criterion (RC) and the Generators Ranging Criterion (GRC) - new (quasi-orthogonal) even BCH-derived sequences are generated which are very attractive for synchronous or quasi-synchronous Code Division Multiple-Access (CDMA) systems. Numerical results show that the new family of BCH-derived sequences can contain a higher number of quasi-orthogonal sequences with lower correlation values and higher processing gains (PGs) than the spreading sequences typically used in the third generation of mobile communications system, UMTS or in the recent large area synchronised CDMA (LAS-CDMA) technology. It is shown that the even BCH-derived sequences are easily generated by a linear shift register generator, allowing the construction of systems with receiver structures of low complexity as compared with those of quasi-synchronous systems using low correlation zone sequences, as for instance the LAS-CDMA system.
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A Non Integer Order Quantum Derivative - Manuel Ortigueira (Symposium on Applied Fractional Calculus (SAFC07), 2007)
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