<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palma, Susana Isabel</style></author><author><style face="normal" font="default" size="100%">Rodrigues, Carlos André</style></author><author><style face="normal" font="default" size="100%">Alexandra Carvalho</style></author><author><style face="normal" font="default" size="100%">M Puerto Morales</style></author><author><style face="normal" font="default" size="100%">Freitas, Filomena</style></author><author><style face="normal" font="default" size="100%">Alexandra R. Fernandes</style></author><author><style face="normal" font="default" size="100%">Cabral, Joaquim Sampaio</style></author><author><style face="normal" font="default" size="100%">Roque, Ana C A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A value-added exopolysaccharide as a coating agent for MRI nanoprobes</style></title><secondary-title><style face="normal" font="default" size="100%">Nanoscale</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://pubs.rsc.org/en/content/articlelanding/2014/nr/c5nr01979f#</style></url></web-urls></urls><pages><style face="normal" font="default" size="100%"> 14272-14283</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Fucopol, a fucose-containing exopolysaccharide (EPS) produced by the bacterium Enterobacter A47 DSM 23139 using glycerol as a carbon source, was employed as a new coating material for iron oxide magnetic nanoparticles (MNP). The coated particles were assessed as nanoprobes for cell labeling by Magnetic Resonance Imaging (MRI). The MNP were synthesized by a thermal decomposition method and transferred to aqueous medium by ligand-exchange reaction with meso-2,3-dimercaptosuccinic acid (DMSA). Covalent binding of EPS to DMSA-stabilized nanoparticles (MNP-DMSA) resulted in a hybrid magnetic-biopolymeric nanosystem (MNP-DMSA-EPS) with a hydrodynamic size of 170 nm, negative surface charge at physiological conditions and transverse to longitudinal relaxivities ratio, r2/r1, of 148. In vitro studies with two human cell lines (colorectal carcinoma - HCT116 - and neural stem/progenitor cells - ReNcell VM) showed that EPS promotes internalization of nanoparticles in both cell lines. In vitro MRI cell phantoms also showed superior performance of MNP-DMSA-EPS in ReNcell VM, for which iron dose-dependent MRI signal drop was obtained at relatively low iron concentrations (12 - 20 µg Fe/ml) and short incubation time. Furthermore, ReNcell VM multipotency was not affected by culture in the presence of MNP-DMSA or MNP-DMSA-EPS for 14 days. Our study suggests that Fucopol-coated MNP represent useful cell labeling nanoprobes for MRI.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">7</style></issue></record></records></xml>