<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Dhadge, VL</style></author><author><style face="normal" font="default" size="100%">Morgado, PI</style></author><author><style face="normal" font="default" size="100%">Freitas, F</style></author><author><style face="normal" font="default" size="100%">Reis, M. A.</style></author><author><style face="normal" font="default" size="100%">A. M. Azevedo</style></author><author><style face="normal" font="default" size="100%">Aires-Barros, R.</style></author><author><style face="normal" font="default" size="100%">Roque, A. C. A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">An extracellular polymer at the interface of magnetic bioseparations</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of the Royal Society Interface</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://rsif.royalsocietypublishing.org/content/11/100/20140743.abstract</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">20140743</style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;FucoPol, a fucose-containing extracellular polysaccharide (EPS) produced by bacterium Enterobacter A47 using glycerol as the carbon source, was employed as a coating material for magnetic particles (MPs), which were subsequently functionalized with an artificial ligand for the capture of antibodies. The performance of the modified MPs (MP–EPS-22/8) for antibody purification was investigated using direct magnetic separation alone or combined with an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and dextran. In direct magnetic capturing, and using pure protein solutions of human immunoglobulin G (hIgG) and bovine serum albumin (BSA), MP–EPS-22/8 bound 120 mg hIgG g−1 MPs, whereas with BSA only 10 ± 2 mg BSA g−1 MPs was achieved. The hybrid process combining both the ATPS and magnetic capturing leads to a good performance for partitioning of hIgG in the desired phase as well as recovery by the magnetic separator. The MPs were able to bind 145 mg of hIgG g−1 of particles which is quite high when compared with direct magnetic separation. The theoretical maximum capacity was calculated to be 410 ± 15 mg hIgG adsorbed g−1 MPs with a binding affinity constant of 4.3 × 104 M−1. In multiple extraction steps, the MPs bound 92% of loaded hIgG with a final purity level of 98.5%. The MPs could easily be regenerated, recycled and re-used for five cycles with only minor loss of capacity. FucoPol coating allowed both electrostatic and hydrophobic interactions with the antibody contributing to enhance the specificity for the targeted products.&lt;/p&gt;
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