<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Roque, A. C. A.</style></author><author><style face="normal" font="default" size="100%">Lowe, C. R.</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">M. Zachariou</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Affinity chromatography: History, Perspectives, Limitations and Prospects</style></title><secondary-title><style face="normal" font="default" size="100%">Affinity Chromatography: Methods and Protocols</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-59745-582-4_1</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%"> Humana Press Inc.</style></publisher><pub-location><style face="normal" font="default" size="100%">U.S.A.</style></pub-location><pages><style face="normal" font="default" size="100%">1-23 </style></pages><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Biomolecule separation and purification has until very recently steadfastly remained one of the more empirical aspects of modern biotechnology. Affinity chromatography, one of several types of adsorption chromatography, is particularly suited for the efficient isolation of biomolecules. This technique relies on the adsorbent bed material that has biological affinity for the substance to be isolated. This review is intended to place affinity chromatography in historical perspective and describe the current status, limitations and future prospects for the technique in modern biotechnology.&lt;/p&gt;
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