<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Barroso, Telma</style></author><author><style face="normal" font="default" size="100%">Temtem, Márcio</style></author><author><style face="normal" font="default" size="100%">Hussain, Abid</style></author><author><style face="normal" font="default" size="100%">Aguiar-Ricardo, Ana</style></author><author><style face="normal" font="default" size="100%">Roque, Ana Cecília A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Preparation and characterization of a cellulose affinity membrane for human immunoglobulin G (IgG) purification</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Membrane Science</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Affinity membrane</style></keyword><keyword><style  face="normal" font="default" size="100%">Biomimetic ligand</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunoglobulins purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Ionic liquid</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">feb</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S0376738809008035</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">1-2</style></number><volume><style face="normal" font="default" size="100%">348</style></volume><pages><style face="normal" font="default" size="100%">224–230</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;This paper reports the design, preparation and characterization of cellulose affinity membranes for antibody purification using a new methodology. Cellulose membranes were prepared from polymer-ionic liquid solutions, namely 1-butyl-3-methylimidazolium chloride {([BMIM][Cl])}, by the water induced phase inversion process. After functionalization with a synthetic ligand 2-(3-aminophenol)-6-(4-amino-1-naphthol)-4-chloro-s-triazine (ligand 22/8), these were evaluated as affinity supports for human immunoglobulin G {(IgG).} Membranes were characterized in terms of morphology {(SEM)}, porosity (mercury porosimetry), hydrophilicity (contact angle measurement), transport properties (permeability) and mechanical performance {(DMA).} Membranes prepared with varying cellulose contents (5 and 10&amp;amp;\#xa0;wt.% cellulose in ionic liquid solutions) lead to films with different properties. The 10&amp;amp;\#xa0;wt.% cellulose membrane presented enhanced morphological and mechanical properties, however, the morphology of this membrane was significantly altered after ligand coupling. Adsorption isotherms for human {IgG} onto 10&amp;amp;\#xa0;wt.% matrix activated with ligand 22/8 were obtained. Preliminary results showed that the bovine serum albumin {(BSA)}, a model impurity, did not adsorb onto the membrane while up to 6&amp;amp;\#xa0;mg {IgG/g} was bound and 2&amp;amp;\#xa0;mg {IgG/g} recovered.&lt;/p&gt;
</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;n/a&lt;/p&gt;
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