<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pina, Ana Sofia</style></author><author><style face="normal" font="default" size="100%">Dias, Ana Margarida G.C.</style></author><author><style face="normal" font="default" size="100%">Ustok, Fatma Isik</style></author><author><style face="normal" font="default" size="100%">Khoury, Graziella El</style></author><author><style face="normal" font="default" size="100%">Cláudia S M Fernandes</style></author><author><style face="normal" font="default" size="100%">Ricardo J F Branco</style></author><author><style face="normal" font="default" size="100%">Lowe, Christopher R.</style></author><author><style face="normal" font="default" size="100%">Roque, Ana C A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mild and cost-effective green fluorescent protein purification employing small synthetic ligands</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Chromatography A</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chromatography</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S0021967315013424</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">1418</style></volume><pages><style face="normal" font="default" size="100%">83 - 93</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Abstract The green fluorescent protein (GFP) is a useful indicator in a broad range of applications including cell biology, gene expression and biosensing. However, its full potential is hampered by the lack of a selective, mild and low-cost purification scheme. In order to address this demand, a novel adsorbent was developed as a generic platform for the purification of \{GFP\} or \{GFP\} fusion proteins, giving \{GFP\} a dual function as reporter and purification tag. After screening a solid-phase combinatorial library of small synthetic ligands based on the Ugi-reaction, the lead ligand (A4C7) selectively recovered \{GFP\} with 94% yield and 94% purity under mild conditions and directly from Escherichia coli extracts. Adsorbents containing the ligand \{A4C7\} maintained the selectivity to recover other proteins fused to GFP. The performance of \{A4C7\} adsorbents was compared with two commercially available methods (immunoprecipitation and hydrophobic interaction chromatography), confirming the new adsorbent as a low-cost viable alternative for \{GFP\} purification.&lt;/p&gt;
</style></abstract><notes><style face="normal" font="default" size="100%">&lt;p&gt;n/a&lt;/p&gt;
</style></notes></record></records></xml>