@article {7567,
	title = {An extracellular polymer at the interface of magnetic bioseparations},
	journal = {Journal of the Royal Society Interface},
	volume = {11},
	year = {2014},
	pages = {20140743},
	abstract = {<p>FucoPol, a fucose-containing extracellular polysaccharide (EPS) produced by bacterium Enterobacter A47 using glycerol as the carbon source, was employed as a coating material for magnetic particles (MPs), which were subsequently functionalized with an artificial ligand for the capture of antibodies. The performance of the modified MPs (MP{\textendash}EPS-22/8) for antibody purification was investigated using direct magnetic separation alone or combined with an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and dextran. In direct magnetic capturing, and using pure protein solutions of human immunoglobulin G (hIgG) and bovine serum albumin (BSA), MP{\textendash}EPS-22/8 bound 120 mg hIgG g-1 MPs, whereas with BSA only 10 {\textpm} 2 mg BSA g-1 MPs was achieved. The hybrid process combining both the ATPS and magnetic capturing leads to a good performance for partitioning of hIgG in the desired phase as well as recovery by the magnetic separator. The MPs were able to bind 145 mg of hIgG g-1 of particles which is quite high when compared with direct magnetic separation. The theoretical maximum capacity was calculated to be 410 {\textpm} 15 mg hIgG adsorbed g-1 MPs with a binding affinity constant of 4.3 {\texttimes} 104 M-1. In multiple extraction steps, the MPs bound 92\% of loaded hIgG with a final purity level of 98.5\%. The MPs could easily be regenerated, recycled and re-used for five cycles with only minor loss of capacity. FucoPol coating allowed both electrostatic and hydrophobic interactions with the antibody contributing to enhance the specificity for the targeted products.</p>
},
	url = {http://rsif.royalsocietypublishing.org/content/11/100/20140743.abstract},
	author = {Dhadge, VL and Morgado, PI and Freitas, F and Reis, M. A. and A. M. Azevedo and Aires-Barros, R. and Roque, A. C. A.}
}
