@article {7074,
	title = {Hybrid monoliths for magnetically-driven protein separations},
	journal = {Adv. Funct. Mater.},
	volume = {24},
	year = {2014},
	pages = {4528{\textendash}4541},
	abstract = {<p>Monoliths represent powerful platforms for isolation of large molecules with high added value. This work presents a hybrid approach for antibody (Ab) capture and release. Using mostly natural polymers and clean processes, it is possible to create macroporous monoliths with well-defined porous networks, tuneable mechanical properties, and easy functionalization with a biomimetic ligand specific for Ab. Magnetic nanoparticles (MNPs) are embedded on the monolith network to confer a controlled magnetic response that facilitates and accelerates Ab recovery in the elution step. The hybrid monolithic systems prepared with agarose or chitosan/poly(vinyl alcohol) (PVA) blends exhibit promising binding capacities of Abs directly from cell-culture extracts (120 {\textpm} 10 mg Ab g-1 support) and controlled Ab magnetically-assisted elution yielding 95 {\textpm} 2\% recovery. Moreover, a selective capture of mAbs directly from cell culture extracts is achieved yielding a final mAb preparation with 96\% of purity.</p>
},
	url = {http://onlinelibrary.wiley.com/doi/10.1002/adfm.201400022/abstract},
	author = {Barroso, Telma and Casimiro, Teresa and Ferraria, A. and Mattioli, F. and Aguiar-Ricardo, Ana and Roque, ACA.}
}
