@article {roque_biocompatible_2009,
	title = {Biocompatible and bioactive gum Arabic coated iron oxide magnetic nanoparticles},
	journal = {Journal of Biotechnology},
	volume = {144},
	number = {4},
	year = {2009},
	note = {<p>n/a</p>
},
	pages = {313{\textendash}320},
	abstract = {<p>The surface modification of iron oxide magnetic nanoparticles {(MNPs)} with gum Arabic {(GA)} via adsorption and covalent coupling was studied. The adsorption of {GA} was assessed during {MNP} chemical synthesis by the co-precipitation method {(MNP\_GA)}, and after {MNP} synthesis on both bare magnetite and {MNP\_GA.} The covalent immobilization of {GA} at the surface of aldehyde-activated {(MNP\_GAAPTES)} or aminated {MNPs} {(MNP\_GAEDC)} was achieved through free terminal amino and carboxylate groups from {GA.} The presence of {GA} at the surface of the {MNPs} was confirmed by {FTIR} and by the quantification of {GA} by the bicinchoninic acid test. Results indicated that the maximum of {GA} coating was obtained for the covalent coupling of {GA} through its free carboxylate groups {(MNP\_GAEDC)}, yielding a maximum of 1.8\&amp;\#xa0;g of {GA} bound/g of dried particles. The hydrodynamic diameter of {MNPs} modified with {GA} after synthesis resulted in the lowest values, in opposition to the {MNPs} co-precipitated with {GA} which presented the tendency to form larger aggregates of up to 1\&amp;\#xa0;μm. The zeta potentials indicate the existence of negatively charged surfaces before and after {GA} coating. The potential of the {GA} coated {MNPs} for further biomolecule attachment was assessed through anchorage of a model antibody to aldehyde-functionalized {MNP\_GA} and its subsequent detection with an {FITC} labeled anti-antibody.</p>
},
	keywords = {Antibody, Biosensing, Bioseparation, gum arabic, Magnetic nanoparticles},
	issn = {0168-1656},
	doi = {10.1016/j.jbiotec.2009.08.020},
	url = {http://www.sciencedirect.com/science/article/pii/S0168165609003897},
	author = {Roque, Ana C A and Bicho, A. and Batalha, Iris L and Cardoso, Ana S. and Hussain, Abid}
}
