@article {barroso_preparation_2010,
	title = {Preparation and characterization of a cellulose affinity membrane for human immunoglobulin G (IgG) purification},
	journal = {Journal of Membrane Science},
	volume = {348},
	number = {1-2},
	year = {2010},
	note = {<p>n/a</p>
},
	month = {feb},
	pages = {224{\textendash}230},
	abstract = {<p>This paper reports the design, preparation and characterization of cellulose affinity membranes for antibody purification using a new methodology. Cellulose membranes were prepared from polymer-ionic liquid solutions, namely 1-butyl-3-methylimidazolium chloride {([BMIM][Cl])}, by the water induced phase inversion process. After functionalization with a synthetic ligand 2-(3-aminophenol)-6-(4-amino-1-naphthol)-4-chloro-s-triazine (ligand 22/8), these were evaluated as affinity supports for human immunoglobulin G {(IgG).} Membranes were characterized in terms of morphology {(SEM)}, porosity (mercury porosimetry), hydrophilicity (contact angle measurement), transport properties (permeability) and mechanical performance {(DMA).} Membranes prepared with varying cellulose contents (5 and 10\&amp;\#xa0;wt.\% cellulose in ionic liquid solutions) lead to films with different properties. The 10\&amp;\#xa0;wt.\% cellulose membrane presented enhanced morphological and mechanical properties, however, the morphology of this membrane was significantly altered after ligand coupling. Adsorption isotherms for human {IgG} onto 10\&amp;\#xa0;wt.\% matrix activated with ligand 22/8 were obtained. Preliminary results showed that the bovine serum albumin {(BSA)}, a model impurity, did not adsorb onto the membrane while up to 6\&amp;\#xa0;mg {IgG/g} was bound and 2\&amp;\#xa0;mg {IgG/g} recovered.</p>
},
	keywords = {Affinity membrane, Biomimetic ligand, Cellulose, Immunoglobulins purification, Ionic liquid},
	issn = {0376-7388},
	doi = {10.1016/j.memsci.2009.11.004},
	url = {http://www.sciencedirect.com/science/article/pii/S0376738809008035},
	author = {Barroso, Telma and Temtem, M{\'a}rcio and Hussain, Abid and Aguiar-Ricardo, Ana and Roque, Ana Cec{\'\i}lia A}
}
