@article {bicho_anti-cd8_2010,
	title = {Anti-CD8 conjugated nanoparticles to target mammalian cells expressing CD8},
	journal = {International Journal of Pharmaceutics},
	volume = {399},
	number = {1-2},
	year = {2010},
	note = {<p>{PMID:} 20696228</p>
},
	pages = {80{\textendash}86},
	abstract = {<p>This work aimed at the development of targeted drug delivery systems using nanoparticles fused with antibodies. The antibody anti-human {CD8} was coupled onto {PLGA} nanoparticles, and the ability of these particles to specifically target cells expressing {CD8} was studied. The obtained particles were found to be of spherical shape exhibiting a size between 350 and 600 nm. In vitro experiments with different cellular cultures {(TE671}, {CHO} and {HEK293)} using unmodified nanoparticles containing rhodamine have shown that particles were present on their surface within 48 h of incubation. In vitro tests using {anti-CD8} conjugated nanoparticles in {CHO} cell cultures indicated that all transfected cells which express {CD8} show these particles on their surface within 1h of incubation. These results demonstrated that, in a shorter time, the produced particles can target cells expressing {CD8} on their surface which offers the ability to reduce drug side effects. The antibody-coupled nanoparticles represent a promising approach to improve the efficacy of active targeting for lymphoblastic leukaemia therapy.</p>
},
	keywords = {Animals, Antibodies, Antigens, Cell Culture Techniques, Cell Line, Cricetinae, Cricetulus, Drug Carriers, Electron, Fluorescence, Fourier Transform Infrared, Humans, Lactic Acid, Microscopy, Monoclonal, nanoparticles, Polyglycolic Acid, Scanning, Spectroscopy, Surface Properties, Transfection, Tumor, {CD8}, {CHO} Cells, {Phase-Contrast}},
	issn = {1873-3476},
	doi = {10.1016/j.ijpharm.2010.08.005},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/20696228},
	author = {Bicho, A. and Pe{\c c}a, In{\^e}s N and Roque, A. C. A. and Cardoso, M Margarida}
}
