@article {pina_comparison_2010,
	title = {Comparison of Fluorescence Labelling Techniques for the Selection of Affinity Ligands from Solid-Phase Combinatorial Libraries},
	journal = {Separation Science and Technology},
	volume = {45},
	number = {15},
	year = {2010},
	note = {<p>n/a</p>
},
	pages = {2187{\textendash}2193},
	abstract = {<p>This study reports the comparison of fluorimetric techniques (fluorescence microscopy and spectrofluorimetry on a 96-well format) for the on-bead screening of combinatorial libraries of affinity ligands for chromatographic separations. Two solid-phase libraries of synthetic ligands based on distinct scaffolds were synthesized by combinatorial chemistry. The libraries comprising ligands representing different hydrophobic/hydrophilic properties and sizes were tested for binding to randomly selected biomolecules (labelled with a fluorophore). Fluorescence microscopy was revealed to be a reliable and reproducible technique for the detection of lead ligands which strongly bound the target biomolecule. Results obtained by fluorescence intensity measurements in a 96-well format were less consistent, mainly due to challenges related with the accurate dispensing of the solid support.</p>
},
	keywords = {Affinity Ligands, Combinatorial Chemistry, Hts},
	doi = {10.1080/01496395.2010.507447},
	author = {Pina, A. S. and Lowe, C. R. and Roque, A. C. A.}
}
