@article {Fernandes2016,
	title = {Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope},
	journal = {Journal of Chromatography A},
	volume = {1438},
	year = {2016},
	note = {<p>n/a</p>
},
	pages = {160{\textendash}170},
	publisher = {Elsevier B.V.},
	abstract = {<p>Retroviral vectors gained popularity toward other viral vectors as they integrate their genome into hosts{\textquoteright} genome, a characteristic required for the modification of stem cells. However, the production of viable particles for gene therapy is hampered by the low ratio of infectious to non-infectious viral particles after purification, low titers and limited number of competent viral receptors. We have developed de novo two fully synthetic triazine-based ligands that can selectively bind retroviral particles pseudotyped with amphotropic murine leukemia virus envelope (AMPHO4070A). A 78-membered library of triazine-based ligands was designed in silico and was virtually screened against the modeled structure of the AMPHO4070A protein. Ligands displaying the highest energy of binding were synthesized on cross-linked agarose and experimentally tested. Adsorbents containing ligands A5A10 and A10A11 showed selectivity toward viral particles containing the target protein (VLP-AMPHO), binding 19 {\textpm} 5 $μ$g/g support and 47 {\textpm} 13 $μ$g/g support, respectively. The elution conditions for both ligands were mild and with high recovery yields (80-100{\%}), in comparison with common purification practices. These results were based on a lab-scale experimental setting with VLP integrity being confirmed through TEM. In particular, the elution buffer containing 12 mM imidazole allowed the recovery of intact amphotropic viral particles.</p>
},
	keywords = {Purification, Triazine scaffold, Viral particles, virtual screening},
	issn = {18733778},
	doi = {10.1016/j.chroma.2016.02.026},
	url = {http://dx.doi.org/10.1016/j.chroma.2016.02.026},
	author = {Cl{\'a}udia S M Fernandes and Rute Castro and Ana Sofia Coroadinha and Roque, Ana C A}
}
