@article {Pina201583,
	title = {Mild and cost-effective green fluorescent protein purification employing small synthetic ligands},
	journal = {Journal of Chromatography A},
	volume = {1418},
	year = {2015},
	note = {<p>n/a</p>
},
	pages = {83 - 93},
	abstract = {<p>Abstract The green fluorescent protein (GFP) is a useful indicator in a broad range of applications including cell biology, gene expression and biosensing. However, its full potential is hampered by the lack of a selective, mild and low-cost purification scheme. In order to address this demand, a novel adsorbent was developed as a generic platform for the purification of \{GFP\} or \{GFP\} fusion proteins, giving \{GFP\} a dual function as reporter and purification tag. After screening a solid-phase combinatorial library of small synthetic ligands based on the Ugi-reaction, the lead ligand (A4C7) selectively recovered \{GFP\} with 94\% yield and 94\% purity under mild conditions and directly from Escherichia coli extracts. Adsorbents containing the ligand \{A4C7\} maintained the selectivity to recover other proteins fused to GFP. The performance of \{A4C7\} adsorbents was compared with two commercially available methods (immunoprecipitation and hydrophobic interaction chromatography), confirming the new adsorbent as a low-cost viable alternative for \{GFP\} purification.</p>
},
	keywords = {Chromatography},
	issn = {0021-9673},
	doi = {http://dx.doi.org/10.1016/j.chroma.2015.09.036},
	url = {http://www.sciencedirect.com/science/article/pii/S0021967315013424},
	author = {Pina, Ana Sofia and Dias, Ana Margarida G.C. and Ustok, Fatma Isik and Khoury, Graziella El and Cl{\'a}udia S M Fernandes and Ricardo J F Branco and Lowe, Christopher R. and Roque, Ana C A}
}
