@article {10330,
	title = {Antimicrobial electrospun silver-, copper- and zinc-doped polyvinylpyrrolidone nanofibers},
	journal = {Journal of Hazardous Materials},
	volume = {299},
	year = {2015},
	pages = {298-305},
	abstract = {<p style="text-align:justify; font-size:12px; font-family:Helvetica">The use of electrospun polyvinylpyrrolidone (PVP) nanofibers containing silver, copper, and zinc nanoparticles was studied to prepare antimicrobial mats using silver and copper nitrates and zinc acetate as precursors. Silver became reduced during electrospinning and formed nanoparticles of several tens of nanometers. Silver nanoparticles and the insoluble forms of copper and zinc were dispersed using low molecular weight PVP as capping agent. High molecular weight PVP formed uniform fibers with a narrow distribution of diameters around 500 nm. The fibers were converted into an insoluble network using ultraviolet irradiation crosslinking. The efficiency of metal-loaded mats against the bacteria Escherichia coli and Staphylococcus aureus was tested for different metal loadings by measuring the inhibition of colony forming units and the staining with fluorescent probes for metabolic viability and compromised membranes. The assays included the culture in contact with mats and the direct staining of surface attached microorganisms. The results indicated a strong inhibition for silver-loaded fibers and the absence of significant amounts of viable but non-culturable microorganisms. Copper and zinc-loaded mats also decreased the metabolic activity and cell viability, although in a lesser extent. Metal-loaded fibers allowed the slow release of the soluble forms of the three metals.</p>
},
	url = {http://dx.doi.org/10.1016/j.jhazmat.2015.06.028},
	author = {Jennifer Quir{\'o}s and Borges, Jo{\~a}o Paulo and Karina Boltes and Ismael Rodea-Palomares and Roberto Rosal}
}
