A bird’s-eye view of denitrification in relation to the nitrogen cycle,
Moura, I., Maia L. B., Pauleta S. R., and Moura J. J. G.
, Metalloenzymes in Denitrification: Applications and Environmental Impacts, RSC Metallobiology Series No. 9 (ISBN: 978-1-78262-376-2)., Cambridge, p.1-10, (2017)
Electron transfer and molecular recognition in denitrification and nitrate dissimilatory pathways,
Almeida, R. M., Dell'Acqua S., Moura I., Pauleta S. R., and Moura J. J. G.
, Metalloenzymes in Denitrification: Applications and Environmental Impacts, RSC Metallobiology Series No. 9 (ISBN: 978-1-78262-376-2)., p.252-286, (2017)
EPR spectroscopy on mononuclear molybdenum-containing enzymes,
Maia, L. B., Moura I., and Moura J. J. G.
, Future Directions in Metalloprotein and Metalloenzyme Research, Biological Magnetic Resonance, Vol. 33 (ISBN: 978-3-319-59100-1), Cham, p.55-101, (2017)
AbstractThe biological relevance of molybdenum was demonstrated in the early 1950s-1960s, by Bray, Beinert, Lowe, Massey, Palmer, Ehrenberg, Pettersson, Vänngård, Hanson and others, with ground-breaking studies performed, precisely, by electron paramagnetic resonance (EPR) spectroscopy. Those earlier studies, aimed to investigate the mammalian xanthine oxidase and avian sulfite oxidase enzymes, demonstrated the surprising biological reduction of molybdenum to the paramagnetic Mo5+. Since then, EPR spectroscopy, alongside with other spectroscopic methods and X-ray crystallography, has contributed to our present detailed knowledge about the active site structures, catalytic mechanisms and structure/activity relationships of the molybdenum-containing enzymes.
This Chapter will provide a perspective on the contribution that EPR spectroscopy has made to some selected systems. After a brief overview on molybdoenzymes, the Chapter will be focused on the EPR studies of mammalian xanthine oxidase, with a brief account on the prokaryotic aldehyde oxidoreductase, nicotinate dehydrogenase and carbon monoxide dehydrogenase, vertebrate sulfite oxidase, and prokaryotic formate dehydrogenases and nitrate reductases.
Insights into nitrous oxide reductase,
Pauleta, S. R., Carreira C., and Moura I.
, Metalloenzymes in Denitrification: Applications and Environmental Impacts, RSC Metallobiology Series No. 9 (ISBN: 978-1-78262-376-2)., p.141-169, (2017)
Insights into the molybdenum/copper heterometallic cluster assembly in the orange protein: probing intermolecular interactions with an artificial metal-binding ATCUN tag,
Maiti, B. K., Almeida R. M., Maia L. B., Moura I., and Moura J. J. G.
, Inorg Chem, Volume 56, p.8900-8911, (2017)
Insights into the recognition and electron transfer steps in nitric oxide reductase from Marinobacter hydrocarbonoclasticus,
Ramos, S., Almeida R. M., Cordas C. M., Moura J. J. G., Pauleta S. R., and Moura I.
, J Inorg Biochem, Volume 177, p.402-411, (2017)