<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Zhang, Z.</style></author><author><style face="normal" font="default" size="100%">Xia, S.</style></author><author><style face="normal" font="default" size="100%">Leonard, D.</style></author><author><style face="normal" font="default" size="100%">Jaffrezic-Renault, N.</style></author><author><style face="normal" font="default" size="100%">Zhang, J.</style></author><author><style face="normal" font="default" size="100%">Bessueille, F.</style></author><author><style face="normal" font="default" size="100%">Goepfert, Y.</style></author><author><style face="normal" font="default" size="100%">Wang, X.</style></author><author><style face="normal" font="default" size="100%">Chen, L.</style></author><author><style face="normal" font="default" size="100%">Zhu, Z.</style></author><author><style face="normal" font="default" size="100%">Zhao, J.</style></author><author><style face="normal" font="default" size="100%">Almeida, M. G.</style></author><author><style face="normal" font="default" size="100%">Silveira, C. M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A novel nitrite biosensor based on conductometric electrode modified with cytochrome c nitrite reductase composite membrane</style></title><secondary-title><style face="normal" font="default" size="100%">Biosensors &amp; Bioelectronics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">Biosensing Techniques/*instrumentation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochromes a1/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytochromes c1/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Electric Conductivity</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrochemistry/*instrumentation</style></keyword><keyword><style  face="normal" font="default" size="100%">Enzymes, Immobilized/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Equipment Design</style></keyword><keyword><style  face="normal" font="default" size="100%">Equipment Failure Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrate Reductases/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrites/*analysis/chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Reproducibility of Results</style></keyword><keyword><style  face="normal" font="default" size="100%">Sensitivity and Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">Water Pollutants, Chemical/*analysis/chemistry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Feb 15</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=18804367</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">6</style></number><edition><style face="normal" font="default" size="100%">2008/09/23</style></edition><volume><style face="normal" font="default" size="100%">24</style></volume><pages><style face="normal" font="default" size="100%">1574-9</style></pages><isbn><style face="normal" font="default" size="100%">1873-4235 (Electronic)0956-5663 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A conductometric biosensor for nitrite detection was developed using cytochrome c nitrite reductase (ccNiR) extracted from Desulfovibrio desulfuricans ATCC 27774 cells immobilized on a planar interdigitated electrode by cross-linking with saturated glutaraldehyde (GA) vapour in the presence of bovine serum albumin, methyl viologen (MV), Nafion, and glycerol. The configuration parameters for this biosensor, including the enzyme concentration, ccNiR/BSA ratio, MV concentration, and Nafion concentration, were optimized. Various experimental parameters, such as sodium dithionite added, working buffer solution, and temperature, were investigated with regard to their effect on the conductance response of the biosensor to nitrite. Under the optimum conditions at room temperature (about 25 degrees C), the conductometric biosensor showed a fast response to nitrite (about 10s) with a linear range of 0.2-120 microM, a sensitivity of 0.194 microS/microM [NO(2)(-)], and a detection limit of 0.05 microM. The biosensor also showed satisfactory reproducibility (relative standard deviation of 6%, n=5). The apparent Michaelis-Menten constant (K(M,app)) was 338 microM. When stored in potassium phosphate buffer (100mM, pH 7.6) at 4 degrees C, the biosensor showed good stability over 1 month. No obvious interference from other ionic species familiar in natural waters was detected. The application experiments show that the biosensor is suitable for use in real water samples.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">18804367</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Zhang, ZhiqiangXia, SiqingLeonard, DidierJaffrezic-Renault, NicoleZhang, JiaoBessueille, FrancoisGoepfert, YvesWang, XuejiangChen, LingZhu, ZhiliangZhao, JianfuAlmeida, M GabrielaSilveira, Celia MEvaluation StudiesResearch Support, Non-U.S. Gov'tEnglandBiosensors &amp;amp; bioelectronicsBiosens Bioelectron. 2009 Feb 15;24(6):1574-9. Epub 2008 Aug 15.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, China.</style></auth-address></record></records></xml>