@article {2660, title = {Isolation and characterization of a rubredoxin and an (8Fe-8S) ferredoxin from Desulfuromonas acetoxidans}, journal = {Biochim Biophys Acta}, volume = {502}, number = {1}, year = {1978}, note = {

0006-3002 (Print)0006-3002 (Linking)Comparative StudyJournal Article

}, month = {Apr 11}, pages = {38-44}, abstract = {

A two cluster (4Fe-4S) ferredoxin and a rubredoxin have been isolated from the sulfur-reducing bacterium Desulfuromonas acetoxidans. Their amino acid compositions are reported and compared to those of other iron-sulfur proteins. The ferredoxin contains 8 cysteine residues, 8 atoms of iron and 8 atoms of labile sulfur per molecule; its minimum molecular weight is 6163. The protein exhibits an abosrbance ratio of A385/A283 = 0.74. Storage results in a bleaching of the chromophore; the denatured ferredoxin is reconstitutable with iron and sulfide. The instability temperature is 52 degrees C. The rubredoxin does not differ markedly from rubredoxins from other anaerobic bacteria.

}, keywords = {Amino Acids/analysis, Bacteria/*analysis, Chemical Phenomena, Chemistry, Drug Stability, Ferredoxins/*isolation \& purification, Iron-Sulfur Proteins, Iron/analysis, Methods, Rubredoxins/*isolation \& purification, Species Specificity, Sulfur/analysis}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=638141 }, author = {Probst, I. and Moura, J. J. and Moura, I. and Bruschi, M. and Legall, J.} }