@article {2635,
	title = {Ferredoxin from Methanosarcina barkeri: evidence for the presence of a three-iron center},
	journal = {Eur J Biochem},
	volume = {126},
	number = {1},
	year = {1982},
	note = {<p>0014-2956 (Print)0014-2956 (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {Aug},
	pages = {95-8},
	abstract = {<p>Methanosarcina barkeri ferredoxin was purified and characterized by electron paramagnetic resonance (EPR) and Mossbauer spectroscopy. The purification procedure included chromatographic steps on DEAE-cellulose and gel filtration. The isolated protein is unstable under aerobic conditions. The ferredoxin exhibits charge transfer bands at 283 nm and 405 nm with an absorption ratio A405/A283 = 0.73. Its molecular weight has been estimated to be 20000-22000 by gel filtration chromatography. The native ferredoxin exhibits an intense EPR signal at g = 2.02 and only a very weak g = 1.94 signal develops upon reduction with dithionite. The Mossbauer spectra of the reduced protein are characteristic of a [3Fe-3S] center. The combined EPR and Mossbauer studies show that M. barkeri ferredoxin contains only [3Fe-3S] clusters, similar to Azotobacter vinelandii Fd[Emptage, M.H., Kent, T.A., Huynh, B.H., Rawlings, J., Orme-Johnson, W.H. \&amp; Munck, M. (1980) J. Biol. Chem. 255, 1793-1796], Desulfovibrio gigas FdII [Huynh, B.H., Moura, J.J.G., Moura, I., Kent, T.A., LeGall, J., Xavier, A.V. \&amp; Munck, E. (1980) J. Biol. Chem. 255, 3242-3244] and mitochondrial beef heart aconitase [Kent, T.A., Dreyer, J.-L., Kennedy, M.C., Huynh, B.H., Emptage, M.H., Beinert, H. \&amp; Munck, E. (1982) Proc. Natl Acad. Sci. USA, 79, 1096-1100].</p>
},
	keywords = {Chemical Phenomena, Chemistry, Electron Spin Resonance Spectroscopy, Euryarchaeota/*analysis, Ferredoxins/*isolation \& purification, Iron/*isolation \& purification, Spectrum Analysis/methods},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=6290216 },
	author = {Moura, I. and Moura, J. J. and Huynh, B. H. and Santos, H. and Legall, J. and Xavier, A. V.}
}
