@article {2578,
	title = {Evidence for selenocysteine coordination to the active site nickel in the [NiFeSe]hydrogenases from Desulfovibrio baculatus},
	journal = {Proc Natl Acad Sci U S A},
	volume = {86},
	number = {1},
	year = {1989},
	note = {<p>0027-8424 (Print)0027-8424 (Linking)Journal ArticleResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.Research Support, U.S. Gov{\textquoteright}t, P.H.S.</p>
},
	month = {Jan},
	pages = {147-51},
	abstract = {<p>Ni and Se x-ray absorption spectroscopic studies of the [NiFeSe]hydrogenases from Desulfovibrio baculatus are described. The Ni site geometry is pseudo-octahedral with a coordinating ligand composition of 3-4 (N,O) at 2.06 A, 1-2 (S,Cl) at 2.17 A, and 1 Se at 2.44 A. The Se coordination environment consists of 1 C at 2.0 A and a heavy scatterer M (M = Ni or Fe) at approximately 2.4 A. These results are interpreted in terms of a selenocysteine residue coordinated to the Ni site. The possible role of the Ni-Se site in the catalytic activation of H2 is discussed.</p>
},
	keywords = {*Nickel, *Selenium/metabolism, Binding Sites, Cysteine/*analogs \& derivatives, Desulfovibrio/*enzymology, Hydrogenase/*metabolism, Iron/metabolism, Metalloendopeptidases/*metabolism, Oxidation-Reduction, Protein Conformation, Selenocysteine, Spectrum Analysis},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=2521386 },
	author = {Eidsness, M. K. and Scott, R. A. and Prickril, B. C. and Dervartanian, D. V. and Legall, J. and Moura, I. and Moura, J. J. and Peck, H. D., Jr.}
}
