@article {2538,
	title = {Primary sequence, oxidation-reduction potentials and tertiary-structure prediction of Desulfovibrio desulfuricans ATCC 27774 flavodoxin},
	journal = {Eur J Biochem},
	volume = {220},
	number = {3},
	year = {1994},
	note = {<p>0014-2956 (Print)0014-2956 (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {Mar 15},
	pages = {987-95},
	abstract = {<p>Flavodoxin was isolated and purified from Desulfovibrio desulfuricans ATCC 27774, a sulfate-reducing organism that can also utilize nitrate as an alternative electron acceptor. Mid-point oxidation-reduction potentials of this flavodoxin were determined by ultraviolet/visible and EPR methods coupled to potentiometric measurements and their pH dependence studied in detail. The redox potential E2, for the couple oxidized/semiquinone forms at pH 6.7 and 25 degrees C is -40 mV, while the value for the semiquinone/hydroquinone forms (E1), at the same pH, -387 mV. E2 varies linearly with pH, while E1 is independent of pH at high values. However, at low pH (\&lt; 7.0), this value is less negative, compatible with a redox-linked protonation of the flavodoxin hydroquinone. A comparative study is presented for Desulfovibrio salexigens NCIB 8403 flavodoxin [Moura, I., Moura, J.J.G., Bruschi, M. \&amp; LeGall, J. (1980) Biochim. Biophys. Acta 591, 1-8]. The complete primary amino acid sequence was obtained by automated Edman degradation from peptides obtained by chemical and enzymic procedures. The amino acid sequence was confirmed by FAB/MS. Using the previously determined tridimensional structure of Desulfovibrio vulgaris flavodoxin as a model [similarity, 48.6\%; Watenpaugh, K.D., Sieker, L.C., Jensen, L.H., LeGall, J. \&amp; Dubourdieu M. (1972) Proc. Natl Acad. Sci. USA 69, 3185-3188], the tridimensional structure of D. desulfuricans ATCC 27774 flavodoxin was predicted using AMBER force-field calculations.</p>
},
	keywords = {Amino Acid Sequence, Base Sequence, Desulfovibrio/*chemistry, Electron Spin Resonance Spectroscopy, Flavodoxin/*chemistry, Hydrogen-Ion Concentration, Molecular Sequence Data, Oxidation-Reduction, Protein Structure, Tertiary, Sequence Homology, Amino Acid, Spectrophotometry, Ultraviolet, Spectrum Analysis},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=8143752 },
	author = {Caldeira, J. and Palma, P. N. and Regalla, M. and Lampreia, J. and Calvete, J. and Schafer, W. and Legall, J. and Moura, I. and Moura, J. J.}
}
