@article {2489,
	title = {Characterization of a 7Fe ferredoxin isolated from the marine denitrifier Pseudomonas nautica strain 617: spectroscopic and electrochemical studies},
	journal = {Biochem Biophys Res Commun},
	volume = {229},
	number = {2},
	year = {1996},
	note = {<p>0006-291X (Print)0006-291X (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {Dec 13},
	pages = {524-30},
	abstract = {<p>A 7Fe ferredoxin, isolated from the marine denitrifier Pseudomonas nautica strain 617, was characterized. The NH2-terminal sequence analysis, performed until residue number 56, shows a high similarity with the 7Fe ferredoxins isolated from Azotobacter vinelandii, Pseudomonas putida, and Pseudomonas stutzeri. EPR and NMR spectroscopies identify the presence of both [3Fe-4S] and [4Fe-4S] clusters, with cysteinyl coordination. The electrochemical studies on [Fe-S] clusters show that a fast diffusion-dominated electron transfer, promoted by Mg(II), takes place between the ferredoxin and the glassy carbon electrode. Square wave voltammetry studies gave access to the electrosynthesis of a 4Fe center formed within the [3Fe-4S] core. The [3Fe-4S] cluster exhibited two reduction potentials at -175 and -680 +/- 10 mV and the [4Fe-4S] cluster was characterized by an unusually low reduction potential of -715 +/- 10 mV, at pH 7.6</p>
},
	keywords = {Amino Acid Sequence, Electrochemistry, Electron Spin Resonance Spectroscopy, Ferredoxins/*chemistry/isolation \& purification, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Pseudomonas/*chemistry, Sequence Homology, Amino Acid},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=8954931 },
	author = {Macedo, A. L. and Besson, S. and Moreno, C. and Fauque, G. and Moura, J. J. and Moura, I.}
}
