@article {2454,
	title = {Enzymatic spectrophotometric determination of nitrites in beer},
	journal = {Analytical Letters},
	volume = {32},
	number = {11},
	year = {1999},
	note = {<p>Times Cited: 6</p>
},
	month = {1999},
	pages = {2217-2227},
	abstract = {<p>A colorimetric assay for nitrite determination in beer based on c-type multiheme enzyme Nitrite reductase (NiR) isolated from Desulfovibrio desulfuricans ATCC 27774, was developed. Using the enzyme in solution, nitrite assay was linear in the 10(-8) - 10(-2) M range with a detection limit of 10(-8) M. and a recovery ranging from 90 to 107\%. The imprecision ranged from 4 to 10\% on the entire calibration curve. With NIR immobilised onto a nylon coil, a flow reactor was developed which showed a narrower linear range (10(-5) - 10(-2) M) and a higher detection limit (10(-5) M) than with the enzyme in solution, but made it possible to reuse the enzyme up to 100 times (50\% residual activity). Sample preparation was simple and fast: only degassing and beer dilution by buffer was needed. This enzymatic assay was in good agreement with the results obtained using commercial nitrite determination kits.</p>
},
	isbn = {0003-2719},
	url = {http://dx.doi.org/10.1080/00032719908542965},
	author = {Girotti, S. and Ferri, E. N. and Fini, F. and Ruffini, F. and Budini, R. and Moura, I. and Almeida, G. and Costa, C. and Moura, J. J. G. and Carrea, G.}
}
