@article {2342,
	title = {Biosensing nitrite using the system nitrite redutase/Nafion/methyl viologen--a voltammetric study},
	journal = {Biosens Bioelectron},
	volume = {22},
	number = {11},
	year = {2007},
	note = {<p>0956-5663 (Print)0956-5663 (Linking)Evaluation StudiesJournal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {May 15},
	pages = {2485-92},
	abstract = {<p>This work describes the construction and voltammetric characterization of a nitrite biosensor based on a cytochrome c-type nitrite reductase (ccNiR) and the Nafion ionomeric matrix loaded with methyl viologen as redox mediator. Despite the potential electrostatic repulsions between the anionic substrate and the Nafion sulfonate groups, the resulting bioelectrode exhibited electrocatalytic activity toward nitrite. This phenomenon must be due to the nonuniformity of the enzyme/Nafion membrane, which allows the direct interaction between the substrate and numerous enzyme molecules. Nevertheless, the anionic nature of Nafion exerted a certain diffusion barrier to nitrite, as revealed by the unusually elevated limits of the linear dynamic range and k(m)(app). The irregularity of the composite membrane also contributed to slow down the rate of charge transfer throughout the Nafion polymer. The level of viologens incorporated within the Nafion membrane had a strong influence in the analytical parameters: as much mediator was present, lower was the sensitivity and wider was the linear range. For an optimized ratio enzyme/mediator the sensitivity was 445+/-8 mA M(-1)cm(-2), within the linear range 75-800 microM; the lowest detected nitrite concentration was 60 microM. The operational stability of the biosensor and the influence of some possible interferences were evaluated.</p>
},
	keywords = {Biosensing Techniques/*methods, Electrochemistry/*methods, Enzymes, Immobilized/chemistry, Fluorocarbon Polymers/*chemistry, Microchemistry/*methods, Nitrite Reductases/*chemistry, Nitrites/*analysis/*chemistry, Paraquat/*chemistry, Reproducibility of Results, Sensitivity and Specificity},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=17101271 },
	author = {Almeida, M. G. and Silveira, C. M. and Moura, J. J.}
}
